Identification des effets de mélanges de PE : fenêtre d'exposition stade de développement, dimorphisme sexuel des cibles biologiques

Dietary exposure in utero and during lactation to a mixture of genistein and an anti-androgen fungicide in a rat mammary carcinogenesis model

Endocrine disruptors may play substantial roles in the high incidence of breast cancer. We previously described how early exposure to the mixture of phytoestrogen genistein (G) and the anti-androgen vinclozolin (V) affects peripubertal mammary development. This study evaluates the carcinogenic potential of exposure to V alone or associated with G from conception until weaning in Wistar rats. Dams were exposed to V, G or GV during pregnancy/lactation. At PND50 offspring were treated with DMBA[7,12-dimethylbenz(a)anthracene]. V or GV maternal exposure decreased number of DMBA-induced mammary tumors in the offspring, without significant modifications in tumor incidence, multiplicity and latenc…

Characterization of rat glutathione transferases in olfactory epithelium and mucus

International audience; The olfactory epithelium is continuously exposed to exogenous chemicals, including odorants. During the past decade, the enzymes surrounding the olfactory receptors have been shown to make an important contribution to the process of olfaction. Mammalian xenobiotic metabolizing enzymes, such as cytochrome P450, esterases and glutathione transferases (GSTs), have been shown to participate in odorant clearance from the olfactory receptor environment, consequently contributing to the maintenance of sensitivity toward odorants. GSTs have previously been shown to be involved in numerous physiological processes, including detoxification, steroid hormone biosynthesis, and am…

Protein expression in submandibular glands of young rats is modified by a high-fat/high-sugar maternal diet

International audience; OBJECTIVE: Maternal diet has consequences on many organs of the offspring, but salivary glands have received little attention despite the importance of the saliva secretory function in oral health and control of food intake. The objective of this work was therefore to document in rats the impact of maternal high-fat/high-sugar diet (Western Diet) on submandibular glands of the progeny. DESIGN: Sprague-Dawley rat dams were fed either a Western diet or control diet during gestation and lactation and their pups were sacrificed 25 days after birth. The pups' submandibular gland protein content was characterized by means of 2D-electrophoresis followed by LC-MS/MS. Data we…

Isoflavonoid-based bone-sparing treatments exert a low activity on reproductive organs and on hepatic metabolism of estradiol in ovariectomized rats

International audience; The use of soy isoflavones is a potential alternative to hormone replacement therapy in post-menopausal bone-loss prevention. Nevertheless, phytoestrogens can target other organs and may disrupt cell proliferation, or could modify endogenous steroid hormone metabolism. These mechanisms could be linked to an increased risk of developing cancer. We therefore studied the possible side effects of such treatments in an experimental model of menopause. Forty adult female Wistar rats were ovariectomized and fed with a genistein-, daidzein- or equol-supplemented diet at bone-sparing levels (10 mg/kg BW/day) for 3 months. The estrogenic effects were assessed by histological a…

Dietary xenoestrogens differentially impair 3T3-L1 preadipocyte differentiation and persistently affect leptin synthesis

International audience; Recent observations have highlighted adipogenesis alterations under exposure to several xenoestrogens at critical stages, and pointed at their possible involvement in the pathogenesis of obesity. However, it remains unclear whether these effects are mediated by classical estrogen receptor (ER) binding and subsequent transcriptional modulation. The aim of this study was to determine the (anti-)adipogenic impact of apigenin, bisphenol A, genistein and 17β-estradiol at the onset of adipose cell maturation, and to correlate it to their estrogenic potential. In steroid-free conditions, 3T3-L1 preadipocytes were induced to differentiate in the presence of xenoestrogens for…

Perception périphérique des signaux sensoriels. 1- Gustation

National audience

Perturbateurs endocriniens et exposition précoce : quel risque pour la mère et l'enfant ?

Elaboration of a method to categorize substances of interest as regards to their potential endocrine disrupting activity: assessment and categorization of prioritized substances

EDCs are substances that alter function(s) of the endocrine system and consequently cause adverse health effects. The endocrine system consists of many cells and tissues that interact with each other and the rest of the body by means of hormones. This system is responsible for controlling a large number of processes in the body from gamete formation, to conception and early developmental processes such as organogenesis, and to most tissue and organ functions throughout life. EDCs interfere with endocrine function by many ways and, in doing so, lead to adverse effects on the health of humans and/or wildlife. Some of the observed health effects associated with EDCs include, butare not limited…

Environmental levels of bisphenol A, genistein and vinclozolin feminize digit length ratios in male rats: towards a new sensitive indicator of prenatal endocrine disruption and its impact in the progeny. – CIME project

Environmental levels of bisphenol A, genistein and vinclozolin feminize digit length ratios in male rats: towards a new sensitive indicator of prenatal endocrine disruption and its impact in the progeny. – CIME project. Colloque PNR-PE

Abnormal peripubertal development of the rat mammary gland following exposure in utero and during lactation to a mixture of genistein and the food contaminant vinclozolin

The impact of early exposure to endocrine disruptor mixtures on mammary gland development is poorly known. Here, we identify the effects of a conception to weaning exposure of rats to the phytoestrogen genistein (G) and/or the antiandrogen vinclozolin (V) at 1 mg/kg-d, alone or in association. Using several approaches, we found that G- and GV-exposed rats displayed significantly greater epithelial branching and proliferation, wider terminal end buds than controls at PND35, as well as ductal hyperplasia and periductal fibrosis. Focal branching defects were present in V-exposed rats. An increased ER and AR expression was observed in G- and CV- as compared to V-exposed rats at PND35. Surprisin…

Plant polyphenols, chemoreception, taste receptors and taste management

International audience; Purpose of review Polyphenols display beneficial health effects through chemopreventive actions on numerous chronic diseases including cancers, metabolic disorders, reproductive disorders and eating behaviour disorders. According to the principle of chemoreception, polyphenols bind cellular targets capable of accepting their stereochemistry, namely metabolizing enzymes and protein receptors, including taste receptors. The extraoral expression of taste receptors and their pharmacological interest in terms of novel drug therapies open up new perspectives on the potential use of these compounds and their interactions with other chemicals in cells. These new perspectives…

Steroid activities comparison of natural and food wrap compounds in human breast cancer cell lines

Abstract In this study, we tested and compared the endocrine disruption activities of compounds in materials used to package foods (bisphenol A, bisphenol F, and bisphenol A diglycidylether BADGE) with natural molecules (genistein, apigenin, kaempferol, and tangeretin) in the human breast cancer cell lines MCF-7 (ER + ) and MDA-MB453 (AR + ; GR + ). Octylphenol was also chosen as a xenoestrogen reference. Two compounds had no estrogenic activity: BADGE and tangeretin. Genistein was the most active compound in the E-Screen assay with MCF-7, followed by octylphenol, bisphenol F, bisphenol A and apigenin, with kaempferol the least potent. All estrogenic compounds competed with 17β-estradiol fo…

Modèles biologiques et mathématiques : de la toxicologie à l'écotoxicologie

CIME: An integrative and multidisciplinary PNR-PE project on Chronic Impact of ED Mixture Exposure at environmental low doses on Reproduction, Development and Behavior

CIME: An integrative and multidisciplinary PNR-PE project on Chronic Impact of ED Mixture Exposure at environmental low doses on Reproduction, Development and Behavior. Colloque PNR-PE

Les effets faibles doses : hypothèse d'action

Rapport d'expertise en tant qu'expert à l'ARET pour le Sénat; Les effets faibles doses : hypothèse d'action

Perturbateurs endocriniens : quels enjeux pour la santé ?

conférence de vulgarisation; absent

Differential effects of perinatal, prepubertal or conception to adulthood exposures to a low dose mixture of genistein and vinclozolin on the genital tract and fertility of male rats and their unexposed progeny

Differential effects of perinatal, prepubertal or conception to adulthood exposures to a low dose mixture of genistein and vinclozolin on the genital tract and fertility of male rats and their unexposed progeny. Colloque PNR-PE

Risques santé liés à l'exposition alimentaire aux PE : phtalates, bisphénol A

Differential Effects of Nonhydroxylated Flavonoids as Inducers of Cytochrome P450 1A and 2B Isozymes in Rat Liver

Flavanone, flavone, and tangeretin differentially affected the activities of cytochrome P540 1A and 2B isozymes in rat liver. Flavone and, to a lesser extent, tangeretin, increased activities of ethoxyresorufin O-deethylase, methoxyresorufin O-demethylase, and pentoxyresorufin O-dealkylase (PROD), whereas flavanone mainly enhanced PROD activity. Immunoblot analysis indicated that flavone and tangeretin increased cytochrome P450 1A1, 1A2, and 2B1,2 forms, whereas flavanone only enhanced the cytochrome P450 2B isozymes. Northern blot study showed that flavone and tangeretin increased the level of the cytochrome P450 1A2 mRNAs. The concentration of the other mRNAs were slightly or not affected…

Consequences of a high sugar/high fat maternal diet on protein expression in young rat salivary glands

Introduction: Early nutrition through maternal diet during pregnancy and breastfeeding was shown to have an impact on obesity in childhood. Experiments on animal models have also indicated alterations regarding taste preferences. In addition, salivary physiology has a documented role in taste preferences and food intake in rodents. The objective of this study was therefore to study the impact of maternal diet on salivary glands’ protein profiles of the offspring.Materials and methods: Female rats received during gestation and lactation either a control (C) or a high fat / high sugar diet (WD for “Western Diet”). 18 offspring rats (9C/9WD) were sacrificed at 25 days of age, i.e. shortly afte…

Consequences of a high sugar/high fat maternal diet on protein expression in young rat submandibular glands

Consequences of a high sugar/high fat maternal diet on protein expression in young rat submandibular glands. Congrès français de Spectrométrie de Masse et d'Analyse Protéomique (SMAP 2015)

Oral homeostasis disruption by medical plasticizer component bisphenol A in adult male rats.

Objectives/Hypothesis Bisphenol A (BPA) is a synthetic estrogen-like chemical mimetic widely used in the manufacture of polycarbonate plastics and epoxy resins found in numerous consumer products including food packaging, medical devices, and dental sealants. Because it is recovered in fluids and it can reach high levels in saliva, this study aimed to evaluate its safety on oral homeostasis by examining its effects on salivary glands, mouth epithelium, water consumption, and salt preference, each parameter being estrogen sensitive. Study Design Randomized controlled trial involving rats. Methods A dose-response study was conducted in adult Wistar rats randomized into five groups (n = 12). B…

[Thu-P1-055] Influence of fermented food consumption on taste and oral microbiota of rats

Oral microbiota varies throughout the individual’s life. These changes are due to several factors (environmental, physiological, lifestyle...) that shape its diversity and composition. However, little is known about the physiological impact of this persistence. Taste perception is one of the possible targeted functions. Indeed, our team has recently established relationships between oral microbiota and taste sensitivity in humans. The objective of this work was to see if fermented food bacteria were able to persist in the oral cavity and consequently influence the oral microbiota and the taste by using a rodent experimental design: two groups of rats (n=27/group) were fed for 3 weeks with e…

Bisphenol A, genistein and vinclozolin: three endocrine disruptors which differentially affect the mammary gland in rats : critical window of exposure

Bisphenol A, genistein and vinclozolin: three endocrine disruptors which differentially affect the mammary gland in rats : critical window of exposure. Colloque PNR-PE

Submandibular salivary glands as an endocrine disruptor target: impact of the period of gensitein/vinclozolin exposure in the Rat

Submandibular salivary glands as an endocrine disruptor target: impact of the period of gensitein/vinclozolin exposure in the Rat. Colloque PNR-PE

Bisphenol A, genistein and vinclozolin: three endocrine disruptors which differentially affect the mammary gland development in rats

Bisphenol A, genistein and vinclozolin: three endocrine disruptors which differentially affect the mammary gland development in rats. 7. Copenhaguen workshop on endocrine disruptors (human environment, lifestyle, and genes in endocrine disruptors)

Bisphénol A et phtalates : sources d’exposition et niveaux d’imprégnation chez les enfants

Revue invitée; absent

Low doses in vivo studies of dietary endocrine disruptor mixtures: Sexual dimorphism relevance of biological target for a trans-generational study in a rodent model

Low doses in vivo studies of dietary endocrine disruptor mixtures: Sexual dimorphism relevance of biological target for a trans-generational study in a rodent model. 1. international toxicology of mixtures conference

Post-weaning xenohormone intake affects adult rat submandibular gland in a sex-dependent manner

International audience; OBJECTIVES: We previously reported that maternal exposure to genistein, vinclozolin, ingested alone or in combination, affects submandibular salivary glands of rats' offspring's. Here, we investigated the responsiveness of submandibular gland when such xenohormone exposure occurs later in life. MATERIALS AND METHODS: Chemicals were given orally to male and female Wistar rats (1mg/kg body weight per day), from weaning to adulthood. Submandibular glands and plasma were collected at postnatal day 100 for histological and molecular analysis. RESULTS: Whereas no effect was observed in females, increases in granular convoluted tubules area coupled with a modification of sa…

La toxico-protéomique : prochaine étape dans l'évolution des biomarqueurs environnementaux ?

Rapport d'expertise de l'ARET pour le Sénat; La toxico-protéomique : prochaine étape dans l'évolution des biomarqueurs environnementaux ?

Perturbateurs endocriniens et métaboliques dans l'alimentation : exposition précoce et santé fertilité - développement - cancer. Troubles sensoriels et comportementaux

Metabolism of apigenin by rat liver phase I and phase II enzymes and by isolated perfused rat liver

The metabolism of apigenin, a low estrogenic flavonoid phytochemical, was investigated in rat using liver models both in vitro (subcellular fractions) and ex vivo (isolated perfused liver). In vitro, phase I metabolism led to the formation of three monohydroxylated derivatives: luteolin which was the major metabolite (K(m) = 22.5 +/- 1.5 microM; V(max) = 5.605 +/- 0.090 nmol/min/mg protein, means +/- S.E.M.), scutellarein, and iso-scutellarein. These oxidative pathways were mediated by cytochrome P450 monooxygenases (P450s). The use of P450 inhibitors and inducers showed that CYP1A1, CYP2B, and CYP2E1 are involved. In vitro studies of phase II metabolism indicated that apigenin underwent co…

Effects of oral Bisphenol A exposure on sweet preference and on submandibular salivary gland structure in male adult rat

La Revue de Gériatrie; Objectifs: Le Bisphénol A (BPA) est un œstrogéno-mimétique qui entre dans la fabrication de plastiques en polycarbonate et de résines époxy destinés entre autres à l'élaboration d'emballages alimentaires, de matériel médical et d'amalgames dentaires. Pouvant atteindre des taux élevés dans la salive, l'objectif de cette étude était de vérifier l'innocuité du BPA au niveau buccal en recherchant les effets sur les préférences gustatives et sur les glandes saliuaires, deux paramètres sensibles aux œstrogènes. Matériels et Méthodes: Une étude effet-dose a été réalisée sur des rats Wistar adultes répartis en 5 groupes (n=12). Le Bisphénol A a été administré pendant 6 semain…

Elaboration of a list of substances of interest as regards to a potential endocrine activity and prioritisation strategy for assessment

Endocrine Disruptor Chemicals (EDCs) are substances that alter function(s) of the endocrine system and consequently cause adverse health effects. The endocrine system consists of many cells and tissues that interact with each other and the rest of the body by means ofhormones. This system is responsible for controlling a large number of processes in the body from gamete formation, to conception and early developmental processes such as organogenesis, and to most tissue and organ functions throughout life. EDCs interfere withendocrine function by many ways and, in doing so, lead to adverse effects on the health of humans and/or wildlife. Some of the observed health effects associated with ED…

Régulation hormonale des glandes salivaires : conséquences d'une exposition en xéno-hormones alimentaires sur les mécanismes apoptiques et proliférateurs impliquées dans le développement et la plasticité des glandes sous maxibulaires

Rapport de fin de contrat de cotutelle de thèse; Régulation hormonale des glandes salivaires : conséquences d'une exposition en xéno-hormones alimentaires sur les mécanismes apoptiques et proliférateurs impliquées dans le développement et la plasticité des glandes sous maxibulaires

perturbateurs endocriniens : altérations comportementales et sensorielles

Effects of genistein, vinclozolin and BPA on the proliferation and differentiation of a human salivary cell line – CIME project

Effects of genistein, vinclozolin and BPA on the proliferation and differentiation of a human salivary cell line – CIME project. Colloque PNR-PE

Neurotoxicité comportementale et toxicité sensorielle

Regulatory and academic studies to derive reference values for human health: The case of bisphenol S

We would like to thank the HBM4EU team including Petra Apel (UBA), Matthieu Meslin and Christophe Rousselle (Anses) for their scientific contribution, as well as the ANSES Working Group on endocrine disruption, and its scientific Anses coordinators François Pouzaud and Sandrine Charles.; International audience; The close structural analogy of bisphenol (BP) S with BPA, a recognized endocrine-disrupting chemical and a substance of very high concern in the European Union, highlights the need to assess the extent of similarities between the two compounds and carefully scrutinize BPS potential toxicity for human health. This analysis aimed to investigate human health toxicity data regarding BPS…

Comportement alimentaire

National audience

Influence of fermented food consumption on oral microbiota and taste perception

Regulatory identification of BPA as an endocrine disruptor : Context and methodology

International audience; BPA is one of the most investigated substances for its endocrine disruptor (ED) properties and it is at the same time in the center of many ED-related controversies, the analysis on how BPA fits to the regulatory identification as an ED is a challenge in terms of methodology. It is also a great opportunity to test the regulatory framework with a uniquely data-rich substance and learn valuable lessons for future cases. From this extensive database, it was considered important to engage in a detailed analysis so as to provide specific and strong evidences of ED while reflecting accurately the complexity of the response as well the multiplicity of adverse effects. An ap…

Abnormal peripubertal development of the rat mammary gland following exposure in utero and during lactation to a mixture of genistein and the food contaminant vinclozolin

The impact of early exposure to endocrine disruptor mixtures on mammary gland development is poorly known. Here, we identify the effects of a conception to weaning exposure of rats to the phytoestrogen genistein (G) and/or the antiandrogen vinclozolin (V) at 1 mg/kg-d, alone or in association. Using several approaches, we found that G- and GV-exposed rats displayed significantly greater epithelial branching and proliferation, wider terminal end buds than controls at PND35, as well as ductal hyperplasia and periductal fibrosis. Focal branching defects were present in V-exposed rats. An increased ER and AR expression was observed in G- and CV- as compared to V-exposed rats at PND35. Surprisin…

Restitution des Travaux d’expertise ANSES du GT-PE relatifs au BPA : Saisines 2009-SA-0331 et 2010-SA-0197 (Résumé de la restitution publique du 8 avril 2013)

Régulation hormonales des perceptions gustatives

Organisateur : Loïc Briand (CSGA) ; sous l'égide de la société des Neurosciences; Nos choix alimentaires sont fortement influencés par nos préférences gustatives. Celles-sont très conditionnées par des facteurs sociaux éducatifs qui, par le biais des coutumes et traditions locales, orientent en partie les phénomènes d’apprentissage et de mémorisations des goûts, ou encore par des facteurs génétiques, le polymorphisme génétique des récepteurs du gout amer en est un exemple. Mais les préférences gustatives sont aussi étroitement liées à la qualité des perceptions, lesquelles découlent de processus physiologiques qui associent les bourgeons du goût et les sécrétions salivaires, ainsi que des s…

Effets d’une exposition orale en Bisphenol A sur la préférence au salé et la structure de la glande sous-mandibulaire chez le rat mâle adulte

Effets d’une exposition orale en Bisphenol A sur la préférence au salé et la structure de la glande sous-mandibulaire chez le rat mâle adulte. Colloque PNR-PE

Endocrine disruptors target collagen type 2 maturation in chondrocytes in vitro – CIME project

Endocrine disruptors target collagen type 2 maturation in chondrocytes in vitro – CIME project. Colloque PNR-PE

A multi-generational study on low-dose BPA exposure in Wistar rats: Effects on maternal behavior, flavor intake and development

Bisphenol A (BPA) is a common endocrine disruptor found as an environmental and food contaminant. It exerts both developmental and behavioral effects, mainly when exposure occurs in early life. The aim of this study was to determine the multi-generational effects of chronic, human-relevant low-dose exposure to BPA on development, maternal behavior and flavor preference in Wistar rats. BPA was orally administered at a daily dose of 5 mu g/kg body weight to FO pregnant dams from the first day of gestation (GD 1) until the last day of lactation (LD 21), and then to Fl offspring from weaning (PND 21) to adulthood (PND 100). F2 offspring were not exposed. Development and clinical signs of toxici…

Dental enamel: a new target of BPA

Dental enamel: a new target of BPA. Journée des jeunes chercheurs

Modification des préférences gustatives et de la plasticité structurale des glandes salivaires par une exposition chronique au bisphenol A chez le rat male adulte

Modification des préférences gustatives et de la plasticité structurale des glandes salivaires par une exposition chronique au bisphenol A chez le rat male adulte. colloque de la SFORL

Effects of BPA and Endocrine Disruptor Mixtures on development and taste preferences in the non-exposed F2 offspring Wistar rats. CIME project

Effects of BPA and Endocrine Disruptor Mixtures on development and taste preferences in the non-exposed F2 offspring Wistar rats. CIME project. Colloque PNR-PE

Estrogenic effects of food wrap packaging xenoestrogens and flavonoids in female Wistar rats : a comparative study

International audience; The objective of this study was to compare the estrogenicity of xenoestrogens found in food wrap packaging and phytoestrogen flavonoids. Uterotrophic and vaginal cornification assays were performed on immature and ovariectomized rats. Genistein, bisphenol F, and octylphenol were identified as estrogenic only in immature rats. Using vaginal cornification as a more specific estrogenic parameter, all tested compounds except tangeretin were active in immature rats. While apigenin and kaempferol appeared to have low estrogenic activity, they potentialized the uterotrophic effect of 17β-estradiol in immature rats. These data showed that (i) phytoestrogens like genistein ca…

Perinatal xenohormone exposure impacts sweet preference and submandibular development in male rats.

Objective To determine the effect of perinatal exposure to low doses of genistein and/or vinclozolin on submandibular salivary gland (SSG) development in juvenile and adult male rats and to establish a link with sweet preference. Material and Methods Female rats received orally (1 mg kg−1 body weight/day) genistein and vinclozolin, alone or in combination, from the first gestational day up to weaning. Sweet preference was assessed at weaning and in adulthood in male offspring; submandibular glands were then collected to study the morphogenesis and mRNA expression of steroid receptors, growth factors and taste related proteins. Results Exposure to genistein and/or vinclozolin resulted in a h…

Endocrine disruptors target chondrogenesis in vivo - CIME project

poster + communication orale; Endocrine disruptors target chondrogenesis in vivo - CIME project. Colloque PNR-PE

Effect of bisphenol A on ameloblasts and tooth enamel mineralization

Effect of bisphenol A on ameloblasts and tooth enamel mineralization. Journées de l’école doctorale

L'expression protéique dans les glandes salivaires est impactée par un régime maternel de type Western-Diet chez le rat

Methodologie, recherche et revue; L'expression protéique dans les glandes salivaires est impactée par un régime maternel de type Western-Diet chez le rat. Journées francophones de nutrition (JFN)

Les addictions

Mechanisms involved in the chemoprevention of flavonoids

Flavonoids, widespread in edible plants, have been studied extensively for their anticarcinogenic properties. However, only few studies have been done with these constituents being administered by the dietary route. In our research, the effects of feeding rats with flavone, flavanone, tangeretin, and quercetin were investigated on two steps of aflatoxin B1 (AFB1)-induced hepatocarcinogenesis (initiation and promotion). Nonpolar flavonoids such as flavone, flavanone and tangeretin administered through the initiation period, decreased the number of -gamma-glutamyl transpeptidase-preneoplastic foci. In the same conditions of administration, quercetin, a polyhydroxylated flavonoid, showed no pr…

Enamel defects reflect perinatal exposure to bisphenol A

Enamel defects reflect perinatal exposure to bisphenol A. Congrès de l’Association pour la recherche en Toxicologie (ARET)

Early endocrine disruptors exposure acts on 3T3-L1 differentiation and endocrine activity

International audience; Introduction: Data from last years suggested that early exposure to endocrine disruptors (EDs) can predispose newborns to endocrine dysfunction of adipocytes, obesity, and associated disorders. The implication of EDs at low doses on adipocyte development has been poorly investigated. For instance, vinclozolin (V) is a dicarboximide fungicide widely used in agriculture since the 90's, alone or in mixture with genistein (G), an isoflavonoid from Leguminosae. This study aims to identify the effect of vinclozolin alone or with genistein, on adipose tissue properties using cell culture.Methods: In steroid-free conditions, 3T3-L1 pre-adipocytes were induced to differentiat…

Evaluation des bénéfices et des risques nutritionnels des édulcorants intenses. Avis de l'Anses

Evaluation des bénéfices et des risques nutritionnels des édulcorants intenses. Avis de l'Anses

Flavonoids of honey and propolis : characterization and effects on hepatic drug-metabolizing enzymes and benzo(a)pyrene-DNA binding in rats

The influence of dietary sunflower honey, propolis, and a flavonoid extract of propolis was examined on drug-metabolizing enzyme activities in rat liver and on microsome-mediated binding of benzo[a]pyrene to DNA. Characterization of flavonoids present in sunflower honey and propolis was achieved in order to assess the relative effects of different components of honey and propolis. Honey and propolis contained the same major flavonoids, pinocembrin, chrysin, galangin, and pinobanksin. The concentration of flavonoids was higher in propolis. Sunflower honey produced no significant changes on phase I and phase II enzyme activities and no modification of in vitro binding of benzo[a]pyrene to DNA…

Effect of three endocrine disruptors, bisphenol a, vinclozolin and genistein, on kallikrein 4 expression. – CIME project

Effect of three endocrine disruptors, bisphenol a, vinclozolin and genistein, on kallikrein 4 expression. – CIME project. Colloque PNR-PE

L’exposition par des mélanges de perturbateurs endocriniens à faibles doses modifie la différenciation adipocytaire – CIME project

L’exposition par des mélanges de perturbateurs endocriniens à faibles doses modifie la différenciation adipocytaire – CIME project. Colloque PNR-PE