0000000000129009

AUTHOR

Hammam I. El Hajj

showing 5 related works from this author

Argan oil prevents down-regulation induced by endotoxin on liver fatty acid oxidation and gluconeogenesis and on peroxisome proliferator-activated re…

2015

In patients with sepsis, liver metabolism and its capacity to provide other organs with energetic substrates are impaired. This and many other pathophysiological changes seen in human patients are reproduced in mice injected with purified endotoxin (lipopolysaccharide, LPS). In the present study, down-regulation of genes involved in hepatic fatty acid oxidation (FAOx) and gluconeogenesis in mice exposed to LPS was challenged by nutritional intervention with Argan oil. Mice given a standard chow supplemented or not with either 6% (w/w) Argan oil (AO) or 6% (w/w) olive oil (OO) prior to exposure to LPS were explored for liver gene expressions assessed by mRNA transcript levels and/or enzyme a…

Peroxisome proliferator-activated receptor gammamedicine.medical_specialtyOO olive oilResearch paper[SDV]Life Sciences [q-bio]Peroxisome proliferator-activated receptorBiologyBiochemistryNuclear receptor 30lcsh:BiochemistryEstrogen-related receptorEstrogen-related receptor alphaInternal medicineACADS acyl CoA dehydrogenase short-chainACADL acyl CoA dehydrogenase long-chainmedicinePGC-1α peroxisome proliferator-activated receptor γ coactivator-1αlcsh:QD415-436ReceptorBeta oxidationHNF-4α hepatic nuclear factor-4αchemistry.chemical_classificationACADM acyl CoA dehydrogenase medium-chainPPARα peroxisome proliferator-activated receptor αERRα estrogen related receptor α[ SDV ] Life Sciences [q-bio]PEPCK phospoenolpyruvate carboxykinaseGluconeogenesisBeta-oxidationGlut4 glucose transporter 4[SDV] Life Sciences [q-bio]G6PH glucose-6-phosphataseEndocrinologyGlut2 glucose transporter 2chemistryNuclear receptorArgan oilAO Argan oilNuclear receptorACOX1 acyl-CoA oxidase 1CoactivatorLPS lipopolysaccharidePeroxisome proliferator-activated receptor alpha
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Peroxisomal and mitochondrial status of two murine oligodendrocytic cell lines (158N, 158JP): potential models for the study of peroxisomal disorders…

2009

International audience; In some neurodegenerative disorders (leukodystrophies) characterized by myelin alterations, the defect of peroxisomal functions on myelin-producing cells (oligodendrocytes) are poorly understood. The development of in vitro models is fundamental to understanding the physiopathogenesis of these diseases. We characterized two immortalized murine oligodendrocyte cell lines: a normal (158N) and a jimpy (158JP) cell line mutated for the proteolipid protein PLP/DM20. Fluorescence microscopy, flow cytometry, and western blotting analysis allow to identify major myelin proteins (PLP colocalizing with mitochondria; myelin basic protein), oligodendrocyte (CNPase and myelin oli…

Proteolipid protein 1BiochemistryMiceMyelinMESH : PhenylbutyratesperoxisomeIsomerasesMESH : Myelin Basic ProteinsEnoyl-CoA HydrataseCell Line TransformedUltrasonographybiologyMESH : Gene Expression RegulationMESH : Myelin Proteolipid Protein3-Hydroxyacyl CoA DehydrogenasesMESH : Myelin-Associated GlycoproteinMESH : Cell Line TransformedPeroxisomeMESH : Multienzyme ComplexesMESH : OligodendrogliaMESH : Enoyl-CoA HydrataseCatalaseFlow CytometryMESH : 3-Hydroxyacyl CoA DehydrogenasesPhenylbutyratesmitochondriaMyelin-Associated GlycoproteinOligodendrogliamyelinMESH : Antineoplastic Agentsmedicine.anatomical_structureMESH : Microscopy Electron TransmissionBiochemistryACOX1MESH : MitochondriaMESH : Acyl-CoA Oxidase2'3'-Cyclic-Nucleotide PhosphodiesterasesMESH : IsomerasesOxidation-ReductionMyelin ProteinsMESH : Flow CytometryAntineoplastic AgentsPeroxisomal Bifunctional EnzymeStatistics NonparametricMyelin oligodendrocyte glycoproteinCellular and Molecular NeuroscienceMicroscopy Electron TransmissionMultienzyme ComplexesMESH : CatalaseMESH : MicePeroxisomesmedicineAnimalsMESH : ATP-Binding Cassette TransportersMyelin Proteolipid ProteinMESH : Statistics Nonparametric[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular BiologyMESH : Oxidation-ReductionMyelin Basic Proteinmurine oligodendrocytesMESH : 2'3'-Cyclic-Nucleotide PhosphodiesterasesPeroxisomal transportOligodendrocyteMyelin basic proteinGene Expression Regulationbiology.proteinATP-Binding Cassette TransportersMyelin-Oligodendrocyte GlycoproteinAcyl-CoA OxidaseMESH : AnimalsMESH : Peroxisomes
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Induction of Mitochondrial Changes Associated with Oxidative Stress on Very Long Chain Fatty Acids (C22:0, C24:0, or C26:0)-Treated Human Neuronal Ce…

2012

In Alzheimer's disease, lipid alterations point towards peroxisomal dysfunctions. Indeed, a cortical accumulation of saturated very long chain fatty acids (VLCFAs: C22:0, C24:0, C26:0), substrates for peroxisomalβ-oxidation, has been found in Alzheimer patients. This study was realized to investigate the effects of VLCFAs at the mitochondrial level since mitochondrial dysfunctions play crucial roles in neurodegeneration. On human neuronal SK-NB-E cells treated with C22:0, C24:0, or C26:0 (0.1–20 μM; 48 h), an inhibition of cell growth and mitochondrial dysfunctions were observed by cell counting with trypan blue, MTT assay, and measurement of mitochondrial transmembrane potential (Δψm) with…

AgingArticle SubjectMitochondrionBiologymedicine.disease_causeBiochemistryMitochondrial apoptosis-induced channelchemistry.chemical_compoundSuperoxidesCell Line TumormedicineHumanslcsh:QH573-671Cell ShapeCell ProliferationMembrane Potential MitochondrialNeuronslcsh:CytologySuperoxideFatty AcidsNeurodegenerationCell BiologyGeneral MedicinePeroxisomeFlow Cytometrymedicine.diseaseMolecular biologyMitochondriaCell biologyOxidative StressProtein SubunitsMicroscopy FluorescencechemistryMultiprotein ComplexesDNAJA3ATP–ADP translocaseOxidative stressResearch ArticleOxidative Medicine and Cellular Longevity
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Biological activities of Schottenol and Spinasterol, two natural phytosterols present in argan oil and in cactus pear seed oil, on murine miroglial B…

2014

International audience; The objective of this study was to evaluate the biological activities of the major phytosterols present in argan oil (AO) and in cactus seed oil (CSO) in BV2 microglial cells. Accordingly, we first determined the sterol composition of AO and CSO, showing the presence of Schottenol and Spinasterol as major sterols in AO. While in CSO, in addition to these two sterols, we found mainly another sterol, the Sitosterol. The chemical synthesis of Schottenol and Spinasterol was performed. Our results showed that these two phytosterols, as well as sterol extracts from AO or CSO, are not toxic to microglial BV2 cells. However, treatments by these phytosterols impact the mitoch…

Argan oilABCA1Biochemistrychemistry.chemical_compoundMice0302 clinical medicineSchottenolBV2 cellspolycyclic compoundsCactus oilATP Binding Cassette Transporter Subfamily G Member 1Liver X ReceptorsMembrane Potential Mitochondrial0303 health sciencesbiologyOpuntiafood and beveragesPhytosterolsOrphan Nuclear ReceptorsSterolsBiochemistryABCG1030220 oncology & carcinogenesisSeeds[PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci]lipids (amino acids peptides and proteins)LXRMicrogliaATP Binding Cassette Transporter 1food.ingredientABCG1LipoproteinsBiophysicsStigmasterol[ PHYS.COND.CM-MS ] Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci]Ficus indicaCell Line03 medical and health sciencesfoodAnimalsPlant OilsLiver X receptorMolecular BiologySpinasterol030304 developmental biologyCholesterolCell BiologySitosterolsSterolSpinasterolchemistryNuclear receptorGene Expression RegulationArgan oilABCA1biology.proteinATP-Binding Cassette Transporters
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Modulation of peroxisomes abundance by argan oil and lipopolysaccharides in acyl-CoA oxidase 1-deficient fibroblasts

2013

Pseudo-neonatal adrenoleukodystrophy (P-NALD) is a neurodegenerative disorder caused by acyl-CoA oxidase 1 (ACOX1) deficiency with subsequent impairment of peroxisomal fatty acid β-oxidation, accumulation of very long chain fatty acids (VLCFAs) and strong reduction in peroxisome abundance. Increase in peroxisome number has been previously suggested to improve peroxisomal disorders, and in this perspective, the present work was aimed at exploring whether modulation of peroxisomes abundance could be achieved in P-NALD fibroblasts. Here we showed that treatment with the natural Argan oil induced peroxisome proliferation in P-NALD fibroblasts. This induction was independent on activations of bo…

food.ingredientChemistryArgan oilPeroxisome ProliferationPeroxisomemedicine.diseaseCell biologyfoodPeroxisomal disordermedicineAcyl-CoA oxidaseACOX1AdrenoleukodystrophyPeroxisome proliferator-activated receptor alphaHealth
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