0000000000284245
AUTHOR
Rabah Zeggari
PADDIAG: Plasmon-Acoustic Device for in vitro Diagnosis
The aim of the project is to develop a plasmon-acoustic device for the real time detection of biomolecular interactions, in the prospect of cancer markers detection in blood samples from patients. In the context of early diagnosis, the detection of infinitesimal quantities of biomarkers is possible thanks to the breakdown of the equilibrium state of biochemical reactions. This could be obtained by the activation of the biological media using a system composed of a microdevice for generating acoustic waves coupled with a fluidic network. The latter system will be integrated on a detection device based on surface plasmon resonance. Here, we present design, realization and characterization of …
Characterization of the Breast Cancer Marker Candidate LAG3 Protein in Human Plasma by Direct SPRi-MALDI-MS Analysis from Antibody Arrays
Proteomics plays an important role in biomarker discovery as prerequisite for later utilization. In this study, we coupled Surface Plasmon Resonance imaging (SPRi) with MALDI-TOF mass spectrometry to permit the multiplexed quantification of binding by SPRi and the molecular characterization of interacting partners by subsequent MS analysis. Hyphenation of the two technologies is applied to the detection and characterization of LAG3 (potential breast cancer biomarker) in human serum1.
Design and fabrication of an acoustic micromixer for biological media activation
International audience; The bioassay of infinitesimal quantities of protein markers in biological samples is the way to early cancer detection. However, this detection can be limited by the diffusion of these macromolecules (analytes) from the bulk to the sensor chip (surface of ligands). Here, we propose a new method to overcome this drawback by the activation of the biological media during the detection step. The principle consists in using ultrasonic vibrations in order to disrupt the equilibrium states of such biomolecular reactions and performing simultaneous detection inside an acoustic micromixer. Technological realization and initial characterizations of the device have been perform…
Automated Cancer Marker Characterization in Human Plasma Using SUrface PLASMON Resonance in Array combined with Mass Spectrometry (SUPRA-MS)
International audience; The combination of Surface Plasmon Resonance technology with Mass Spectrometry becomes a key method for the characterization of targeted proteins in the fields of diagnosis and functional proteomics. We demonstrated in this work the ability of our SPRi-chip to capture targeted protein in biological fluids and in situ analyze by MS and MS/MS modes through automated procedure to go beyond classical immunoassays. Here, we established a proof of concept of SUPRA-MS for the detection, the identification and the characterization of a potential breast cancer marker.