Greenhouse Gas Emissions from Wastewater Treatment Plants on a Plantwide Scale: Sensitivity and Uncertainty Analysis

This paper presents the sensitivity and uncertainty analysis of a mathematical model for greenhouse gas emission (GHG) and energy consumption assessment in wastewater treatment plants. A sensitivity analysis was carried out (using two different methods) to determine which model factors have the greatest effect on the predicted values of the GHG production. Further, an uncertainty analysis was carried out to quantify the uncertainty of the key model outputs, such as carbon dioxide production from activated sludge treatment. The results show that influent fractionation factors, which characterize influent composition, have an important role on direct and indirect GHGs production and emission.…

Modelling biological nitrogen and phosphorus removal with soluble microbial products (SMP) production-degradation processes

Over the last two decades, Membrane Bioreactors (MBR) are increasingly used for wastewater treatment. Mathematical modelling of MBR systems has played a key role in order to better explain the effect of their peculiarities. Indeed, several MBR models have been presented in literature in order to improve the knowledge on MBR systems: biological models, hybrid models which include soluble microbial product (SMP) modelling, physical models able to describe the membrane fouling and integrated models which couple the hybrid models with the physical ones. However, among the existing MBR models only few integrated models have been developed which take into account the existing relationship between…

Uncertainty of a biological nitrogen and phosphorus removal model

In the last few years, the use of mathematical models in wastewater treatment plant (WWTP) processes has become a common way to predict WWTP behaviour. However, mathematical models generally demand advanced input for their implementation that must be evaluated by an extensive data gathering campaign, which cannot always be carried out. This fact, together with the intrinsic complexity of the model structure, leads to model results that may be very uncertain. Quantification of the uncertainty is imperative. However, despite the importance of uncertainty quantification, only a few studies have been carried out in the wastewater treatment field, and those studies only included a few of the sou…

Bacterial Community Structure of an IFAS-MBRs Wastewater Treatment Plant

TIn this work, the bacterial community putatively involved in BNR events of a UCT-MBMBR pilot plant was elucidated by both culture-dependent and metagenomics DNA analyses. The presence of bacterial isolates belonging to Bacillus (in the anoxic compartment) and to Acinetobacter, Stenotrophomonas, Rhodococcus, Escherichia and Aeromonas (in the aerobic compartment) is in agreement with the nitrification/denitrification processes observed in the plant. Moreover, the study of bacterial community structure by NGS revealed a microbial diversity suggesting a biochemical complexity which can be further explored and exploited to improve UCT-MBMBR plant performance.

A plant-wide wastewater treatment plant model for carbon and energy footprint: Model application and scenario analysis

Abstract A new model for accounting carbon and energy footprint of wastewater treatment plants (WWTPs) is proposed. The model quantifies direct and indirect greenhouse gas (GHG) emissions related to biological and physical processes of a WWTP. The model takes into account several innovative aspects with respect to already available literature models: i. kinetic/mass-balances; ii. nitrification as a two-step process; iii. nitrous oxide (N2O) formation during nitrification and denitrification both in dissolved and off-gas forms. A full-scale application has been performed by adopting the case study of a real WWTP. A scenario analysis was performed to quantify the influence of: composition of …

Minimizing membrane bioreactor environmental footprint by multiple objective optimization.

This paper presents a modelling study aimed at minimizing the environmental foot print of a membrane bioreactor (MBR) for wastewater treatment. Specifically, an integrated model for MBR was employed in view of the management optimization of an MBR biological nutrient removal (BNR) pilot plant in terms of operational costs and direct greenhouse gases emissions. The influence of the operational parameters (OPs) on performance indicators (PIs) was investigated by adopting the Extended-FAST sensitivity analysis method. Further, a multi-objective analysis was performed by applying the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS). The results show-up that the sludge …

New applications in integrated fixed film activated sludge-membrane bioreactor (IFAS-MBR) systems

Abstract This chapter provides an overview on the development and application of integrated fixed film activated sludge-membrane bioreactor (IFAS-MBR) systems. IFAS-MBRs represent a novel configuration for advanced wastewater treatment, and only a few studies are available in literature. With this aim, the results of some studies on IFAS-MBR systems already published are discussed and compared in this chapter. Literature shows that the influent carbon to nitrogen ratio (C/N) strongly affects the biological process in IFAS-MBR. The organic matter removal efficiency (removed biologically) decreases from 60% to 23.5% with the decrease of influent C/N from 10 to 2 mgCOD mgTN− 1. Nitrogen remova…

Influence of volatile solids and pH for the production of volatile fatty acids: batch fermentation tests using sewage sludge

The aim of this work was to study the effect of volatile suspended solid (VSS) and pH on volatile fatty acids (VFA) production from waste activated sludge (WAS) fermentation by means of batch tests. The final goal was to gain insights to enhance VFA stream quality, with the novelty of using WAS with high sludge retention time. Results revealed that the optimum conditions to maximize VFAs and minimize nutrients and non-VFA sCOD are a VSS concentration of 5.9 g/L and initial pH adjustment to pH 10. The WAS bacterial community structures were analysed according to Next Generation Sequencing (NGS) of 16S rDNA amplicons. The results revealed changes of bacterial phyla abundance in comparison wit…

Occurrence of illicit drugs in two wastewater treatment plants in the South of Italy

In this study the occurrence and the behavior of illicit drugs and their metabolites have been investigated for two wastewater treatment plants (WWTPs) (namely, WWTP-1 and WWTP-2) located in Sicily (island of Italy). Samples were analyzed for methamphetamine, cocaine (COC), 3,4-methylenedioxymethamphetamine (MDMA), methadone (METH), 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), 3,4-methylenedioxy amphetamine (MDA); 3,4-methylenedioxy ethylamphetamine (MDEA), 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) and Benzoylecgonine (BEG). The BEG, COC, MOR and THC-COOH were found at the highest concentration in both WWTPs. The Wastewater-based epidemiology calculation for BEG, COC…

Mathematical modelling of greenhouse gas emissions from membrane bioreactors: A comprehensive comparison of two mathematical models.

Abstract This paper compares two mathematical models (Model I and Model II) to predict greenhouse gases emission from a University Cape Town (UCT) – membrane bioreactor (MBR) plant. Model I considers N 2 O production only during denitrification. Model II takes into account the ammonia-oxidizing bacteria (AOB) formation pathways for N 2 O. Both models were calibrated adopting real data. Model comparison was performed in terms of (i) sensitivity analysis (ii) best fit and (iii) model prediction uncertainty. On average 6% of factors of Model I and 9% of Model II resulted to be important. In terms of best fit, Model II had a better capability of reproducing the measured data. The average effici…

A mathematical model for a sequential batch membrane bioreactor pilot plant

A mathematical model to quantify the nitrogen removal for a membrane bioreactor (MBR) has been presented in this study. The model has been applied to a pilot plant having a pre-denitrification MBR scheme. The pilot plant was cyclically filled with real saline wastewater according to the fill-draw-batch operation. The model was calibrated by adopting a specific protocol based on extensive field dataset. The Standardized Regression Coefficient (SRC) method was adopted to select the most influential model factors to be calibrated. Results related to the SRC method have shown that model factors of the efficiency of backwashing and the biological factors affecting the soluble microbial products …

Greenhouse gases from membrane bioreactor treating hydrocarbon and saline wastewater

The effect of wastewater salinity and presence of petroleum hydrocarbon on N2O emission was investigated in a membrane bioreactor, in which the anoxic and aerobic zones were put in series according to a pre-denitrification scheme. The pilot plant, was continuously fed by a mixture of real and synthetic wastewater. It was operated with a first phase of acclimation of the biomass to a given salinity by gradually increasing the salt concentration from 10 gNaCl/L to 20 gNaCl/L, and to a second phase of petroleum hydrocarbon dosing at 2 g/L (as gasoline). The first phase revealed a clear relationship between nitrous oxide emissions and salinity due to the increased NO2-N production caused by the…

The effect of the solids and hydraulic retention time on moving bed membrane bioreactor performance

Abstract The aim of the present paper was to investigate the effect of solids (SRT) and hydraulic (HRT) retention time on Integrated Fixed Film Activated Sludge (IFAS) University of Cape Town (UCT) membrane Bioreactor (MBR). In particular, three different pairs of SRT and HRT values were analysed, namely, Phase I 56 d/30 h, Phase II 31 d/15 h and Phase III 7 d/13 h. The short-term effect of these three SRT/HRT conditions was assessed by analysing several system performance indicators: organic carbon and biological nutrient (nitrogen and phosphorus) removal, biomass respiratory activity, activated sludge filtration properties and membrane fouling. The results showed that the decrease of SRT/…

Moving bed membrane bioreactors for carbon and nutrient removal: The effect of C/N variation

In this paper, an experimental campaign was carried out on a University of Cape Town Integrated Fixed Film Activated Sludge Membrane Bioreactor (UCT-IFAS-MBR) pilot plant. The aim of the study was to evaluate the effect of the influent C/N ratio on the system performance in terms of organic carbon, nitrogen and phosphorus removal, biomass viability (through respirometry), activated sludge features and membrane filtration properties. The experiments were organized into three phases, characterized by a variation of the C/N ratio (namely, Phase I: C/N= 5, Phase II: C/N =10; Phase III: C/N = 2). The results highlighted that the system performance was significantly affected by C/N ratio. The rem…

Simplified model to evaluate the fate of micropollutants in an integrated urban drainage system: sensitivity analysis

The paper presents the sensitivity analysis of an integrated urban water quality system by means of the global sensitivity analysis (GSA). Specifically, an home-made integrated model developed in previous studies has been modified in order to include the micropollutant assessment (namely, sulfamethoxazole - SMX). The model takes into account also the interactions between the three components of the system: sewer system (SS), wastewater treatment plant (WWTP) and the receiving water body (RWB). The analysis has been applied to an experimental catchment nearby Palermo (Italy): the Nocella catchment. Five scenarios each characterized by different combinations of sub-systems (i.e., SS, WWTP and…

A practical protocol for calibration of nutrient removal wastewater treatment models

Activated sludge models can be very useful for designing and managing wastewater treatment plants (WWTPs). However, as with every model, they need to be calibrated for correct and reliable application. Activated sludge model calibration is still a crucial point that needs appropriate guidance. Indeed, although calibration protocols have been developed, the model calibration still represents the main bottleneck to modelling. This paper presents a procedure for the calibration of an activated sludge model based on a comprehensive sensitivity analysis and a novel step-wise Monte Carlo-based calibration of the subset of influential parameters. In the proposed procedure the complex calibration i…

The role of EPS in fouling and foaming phenomena occurring in an UCT-MBR pilot plant

The aim of this study is to detect a possible relationship between EPS and fouling and EPS and foaming in a University Cape Town (UCT) membrane bioreactor (MBR) pilot plant. The UCT-MBR pilot plant was fed with 40 L/h of real wastewater and monitored for 165 days. Specifically physical/chemical features of influent, permeate and mixed liquor in different sections were analyzed. The fouling phenomenon was studied monitoring the hydraulic resistances of the membrane. The extracellular polymeric substances (EPSs) concentration inside the aerobic tank were also measured. The foaming phenomenon was monitored by performing the Foam Power and Scum Index testes. Results have shown a high correlatio…

OSSERVATORIO SUGLI IMPIANTI DI DEPURAZIONE MBR - Rapporto 2016

I reattori biologici a membrana (MBR, dall’inglese Membrane Biological Reactor) derivano dall’accoppiamento dei tradizionali processi a biomassa sospesa con i processi di filtrazione su membrane microporose o ultraporose, a seconda delle dimensioni nominali dei pori. I maggiori vantaggi legati a questa tecnologia vanno ricercati nella possibilità di eliminare, a valle della fase biologica, quella dei vincoli gestionali ed operativi a quest’ultima connessi. La sostituzione della sedimentazione con una fase di filtrazione su membrane comporta: una notevole riduzione dell’ingombro planimetrico dell’impianto di depurazione, dovuta sia alla eliminazione delle unità di sedimentazione che alla d…

Nitrous oxide emissions in a membrane bioreactor treating saline wastewater contaminated by hydrocarbons

The joint effect of wastewater salinity and hydrocarbons on nitrous oxide emission was investigated. The membrane bioreactor pilot plant was operated with two phases: i. biomass acclimation by increasing salinity from 10 gNaCl L−1 to 20 gNaCl L−1 (Phase I); ii. hydrocarbons dosing at 20 mg L−1 with a constant salt concentration of 20 gNaCl L−1 (Phase II). The Phase I revealed a relationship between nitrous oxide emissions and salinity. During the end of the Phase I, the activity of nitrifiers started to recover, indicating a partial acclimatization. During the Phase II, the hydrocarbon shock induced a temporary inhibition of the biomass with the suppression of nitrous oxide emissions. The r…

Global sensitivity analysis in wastewater treatment modelling

Global sensitivity analysis (GSA) is a valuable tool to support the use of mathematical models. GSA allows the identifcation of the effect of model and input factor uncertainty on the model response, also considering the effect due to the interactions among factors. During recent years, the wastewater modelling feld has embraced the use of GSA. Wastewater modellers have tried to transfer the knowledge and experience from other disciplines and other water modelling felds.

Nutrient recovery from wastewater treatment by ultrafiltration membrane for water reuse in view of a circular economy perspective

The study aims to recover nitrogen from wastewater by employing ultrafiltration membrane in water reuse for agriculture purpose. To such aim, a new reclaimed water quality index (RWQI) is proposed and applied including an innovative protocol for its assessment. Specifically, the influence of filtration and backwashing times for an ultrafiltration system aimed to nutrient recovery has been analyzed. The final goal was to pin down the trade-off between operation costs and effluent quality. Results show that backwashing time play a crucial role in reducing the operation costs; indeed, low values (i.e., 0.5 min) lead to an increase in the number of required chemical cleanings and consequently o…

BIOLOGICAL STABILITY ANALYSIS OF THE OFMSW MATRIX TREATED IN A COMPOSTING PLANT

Composting, mechanical-biological treatment (MBT), biodrying and biostabilization processes of pre-selected organic fraction from municipal solid wastes (OFMSW) have recently become important in municipal solid waste management. Through these treatment processes the potential impacts of the produced wastes and the volume to treat in the landfill are strongly reduced. However, the residual organic matter contained in the treated fraction may provide negative effects when e.g. the treated OFMSW is used as compost. The biological stability degree of the treated matrix provides usefull informations for the final use of it. Indeed, knowing the degree of biological stability of the compost makes …

The role of EPS in fouling and foaming phenomena for a membrane bioreactor

In contraposition to conventional activated sludge processes, the foaming phenomenon in membrane bioreactor (MBR) is still in its infancy. On the other hand, although several studies have been carried out for better understanding the fouling phenomenon in MBR there are still some gaps in the up-to-date knowledge. The extracellular polymeric substances (EPSs) may have a primary role in fouling and foaming phenomena which in turn can be crucial for MBRs. The aim of this study is to detect a possible relationship that EPSs may have with fouling and foaming in an MBR for wastewater treatment. Foaming phenomenon is monitored by performing specific foam-tests: Foam Power, Scum Index, Foam Rating …

Evaluation of greenhouse gas emissions from membrane bioreactors: development of a new mathematical model

Biological nutrient removal in a UCT-MBR pilot

In the last years, there has been an increasing awareness about the environment pollution protection. As a consequence, the Environmental Regulation has increased the emission limits imposing, for instance, lower concentrations at the wastewater treatment plant (WWTP) outlets. As a consequence of this fact, several WWTPs are not able to respect the emission limits and need to be upgraded. In view of such needs new technology are emerging and new WWTP solution schemes are being realized. With respects to the nutrient removal enhancing, a possible solution can be the UCT-MBR which couples the University of Cape Town scheme with a Membrane Bioreactor. In the light of such considerations, the s…

MODELLAZIONE DEI PROCESSI DI RIMOZIONE DEI NUTRIENTI IN IMPIANTI UCT-MBR

Comparison of Two Mathematical Models for Greenhouse Gas Emission from Membrane Bioreactors

In this study two mathematical models (Model I and Model II), able to predict the nitrous oxide (N2O) and carbon dioxide (CO2) emission from an University Cape Town (UCT) – membrane bioreactor (MBR) plant, have been compared. Model I considers the N2O production only during the denitrification. Model II takes into account the two ammonia-oxidizing bacteria (AOB) formation pathways for N2O. Both models were calibrated adopting real data. Results highlight that Model II had a better capability of reproducing the measured data especially in terms of N2O model outputs. Indeed, the average efficiency related to the N2O model outputs was equal to 0.3 and 0.38 for Model I and Model II respectively.

Biological Nutrient Removal and Fouling Phenomena in a University of Cape Town Membrane Bioreactor Treating High Nitrogen Loads

The behavior of a University of Cape Town (UCT) membrane bioreactor (MBR) system was investigated for use in biological nutrient removal from real wastewater. The pilot plant was in operation for a period of 165 days, during which an extensive data gathering campaign was conducted. The pilot plant was started up by inoculating it with activated sludge from a nearby wastewater treatment plant, and it was fed by real municipal wastewater characterized by high organic nitrogen concentrations attributable to discharges from industrial wastewater and sporadic landfill leachate. Carbon and biological nutrient removal processes, a sludge production process, and a membrane fouling mechanism were an…

Greenhouse gases from sequential batch membrane bioreactors: A pilot plant case study

Abstract The paper reports the results of nitrous oxide (N 2 O) emissions from aerobic and anoxic tank of a Sequential Batch Membrane Bioreactor (SB-MBR) pilot plant. The influence of salinity variation on N 2 O emission was analyzed by gradually increasing the inlet salt concentration from 0 to 10 g NaCl L −1 . The observed results showed that the N 2 O concentration of the gaseous samples was strongly influenced by the salt concentration. This result was likely related to a worsening of the nitrification activity due to the effect of salinity on autotrophic bacteria. Dissolved oxygen concentration and salinity were found to be the key factors affecting N 2 O concentration in the gaseous s…

Destino dei radionuclidi negli impianti di depurazione delle acque reflue urbane

Lo studio presentato in questo lavoro ha avuto come obiettivo il monitoraggio e l’analisi del destino dei radionuclidi negli impianti di depurazione ed è stato condotto in collaborazione tra l’Università di Palermo e ARPA Sicilia. Esso ha previsto lo svolgimento di due fasi sperimentali. Nel corso della prima sono stati monitorati gli impianti di depurazione a servizio dei Comuni di Palermo (in località Acqua dei Corsari e Fondo Verde), Bagheria e Cefalù; nella seconda fase l’indagine è stata limitata al solo impianto di Acqua dei Corsari, nel quale si erano riscontrati in precedenza i maggiori spunti di interesse. In entrambe le fasi sono stati prelevati campioni istantanei in diverse sezi…

La metodica wastewater based epidemiology come proxy di quantificazione del consumo di sostanze stupefacenti nella popolazione: l’esperienza della città di Palermo.

INTRODUZIONE Le istituzioni europee e la comunità scientifica hanno, recentemente, definito un nuovo modello di sorveglianza attiva della potenziale diffusione delle droghe nella collettività, la Wastewater Based Epidemiology (WBE), attraverso la ricerca analitica delle molecole e dei loro metaboliti nelle acque reflue cittadine. Lo scopo di questo lavoro è stato la valutazione di una preliminare applicazione di METODIca WBE nella città di Palermo, poco studiata in precedenti analisi a livello nazionale e internazionale. METODI Sono stati prelevati 28 campioni (2 litri cad) di refluo medio delle 24 ore in ingresso ai due depuratori di Palermo (WP1=45.000 ab. eq.; WP2=330.000 ab. eq.) in un …

Uncertainty and sensitivity analysis for reducing greenhouse gas emissions from wastewater treatment plants.

Abstract This paper presents the sensitivity and uncertainty analysis of a plant-wide mathematical model for wastewater treatment plants (WWTPs). The mathematical model assesses direct and indirect (due to the energy consumption) greenhouse gases (GHG) emissions from a WWTP employing a whole-plant approach. The model includes: i) the kinetic/mass-balance based model regarding nitrogen; ii) two-step nitrification process; iii) N2O formation both during nitrification and denitrification (as dissolved and off-gas concentration). Important model factors have been selected by using the Extended-Fourier Amplitude Sensitivity Testing (FAST) global sensitivity analysis method. A scenario analysis h…

Micropollutants throughout an integrated urban drainage model: Sensitivity and uncertainty analysis

Abstract The paper presents the sensitivity and uncertainty analysis of an integrated urban drainage model which includes micropollutants. Specifically, a bespoke integrated model developed in previous studies has been modified in order to include the micropollutant assessment (namely, sulfamethoxazole – SMX). The model takes into account also the interactions between the three components of the system: sewer system (SS), wastewater treatment plant (WWTP) and receiving water body (RWB). The analysis has been applied to an experimental catchment nearby Palermo (Italy): the Nocella catchment. Overall, five scenarios, each characterized by different uncertainty combinations of sub-systems (i.e…

Comparison between two MBR pilot plants treating synthetic shipboard slops: effect of salinity increase on biological performance, biomass activity and fouling tendency

The paper reports the main results of an experimental campaign carried out on two bench scale pilot plants for treatment of synthetic shipboard slops. In particular, two membrane bioreactors (MBRs) with submerged configuration were analyzed. One MBR pilot plant (namely, Line A) was fed with synthetic shipboard slop and was subjected to a gradual increase of salinity. Conversely, the second MBR pilot plant (namely, Line B) was fed with the same synthetic shipboard slop but without salt addition, therefore operating as a “control” unit. Organic carbon, hydrocarbons and ammonium removal, kinetic constants, extracellular polymeric substances (EPSs) production and membranes fouling rates have be…

Quantifying sensitivity and uncertainty analysis of a new mathematical model for the evaluation of greenhouse gas emissions from membrane bioreactors

Abstract A new mathematical model able to quantify greenhouse gas (GHG) emissions in terms of carbon dioxide (CO 2 ) and nitrous oxide (N 2 O) for a Membrane Bioreactor (MBR) is presented. The proposed mathematical model is of the Activated Sludge Model (ASM) family and takes into account simultaneously both biological and physical processes (e.g., membrane fouling). An analysis of the key factors and sources of uncertainty influencing GHG emissions is also presented. Specifically, the standardized regression coefficient, the Extended-FAST and a Monte Carlo based method are employed for assessing model factors which influence three performance indicators: effluent quality index, operational…

Biological nitrogen and phosphorus removal in membrane bioreactors: model development and parameter estimation

Membrane bioreactors (MBR) are being increasingly used for wastewater treatment. Mathematical modeling of MBR systems plays a key role in order to better explain their characteristics. Several MBR models have been presented in the literature focusing on different aspects: biological models, models which include soluble microbial products (SMP), physical models able to describe the membrane fouling and integrated models which couple the SMP models with the physical models. However, only a few integrated models have been developed which take into account the relationships between membrane fouling and biological processes. With respect to biological phosphorus removal in MBR systems, due to th…

Enhancing a Transition to a Circular Economy in the Water Sector: The EU Project WIDER UPTAKE

A novel approach for resource recovery includes forward osmosis (FO) as a concentration step in municipal wastewater treatment. The current study investigates different pre-treatment strategies including biological treatment with a moving-bed bioreactor (MBBR) at different loading rates and particle removal by filtration and sedimentation. Membrane performance and recovery potential for energy and nutrients were investigated in laboratory-scale FO experiments in batch mode using pre-treated municipal wastewater as feed and 35 g/L NaCl as a draw solution. Initial water fluxes were in the range of 6.3 to 8.0 L/(m2·h). The baseline fluxes were modelled to account for flux decline due to concen…

Radionuclides in wastewater treatment plants: monitoring of Sicilian plants.

Three Sicilian wastewater treatment plants were monitored to assess the occurrence and the behaviour of radionuclides. Two sampling campaigns (screening and long-term) were carried out during which liquid and solid samples have been analysed. It was found that 131I mostly occurred in the samples analysed during the screening campaign (43% of the analysed samples contained 131I). High 131I specific activity was found in the mixed liquor, recycled sludge and dehydrated sludge samples. This finding was mainly due to the tendency of 131I to be associated with solid particles. During the long-term sampling campaign an influence of the sludge retention time (SRT) on the 131I behaviour was found. …

A comprehensive integrated membrane bioreactor model for greenhouse gas emissions

Abstract A comprehensive integrated membrane bioreactor (MBR) model for wastewater treatment is here proposed. The model quantifies the main biological and physical processes. The model describes the biological removal of organic matter, nitrogen and phosphorus including greenhouse gases (carbon dioxide, CO 2 and nitrous oxide, N 2 O). The model takes into account the following main innovative aspects jointly: i. Two-step nitrification process; ii. N 2 O formation due to ammonia-oxidizing bacteria as a product of the hydroxylamine oxidation (NH 2 OH) and of the nitrite (NO 2 − ) reduction; iii. Soluble microbial product (SMP) formation/degradation due to microbial growth and endogenous resp…

Sensitivity and uncertainty analysis of a plant-wide model for carbon and energy footprint of wastewater treatment plants

This paper presents the sensitivity and uncertainty analysis of a mathematical model for Greenhouse gas (GHG) and energy consumption assessment from wastewater treatment plants (WWTPs). The model is able to simultaneously describe the main biological and physical-chemical processes in a WWTP. Specifically, the mathematical model includes the main processes of the water and sludge lines influencing the methane (CH4), nitrous oxide (N2O), and carbon dioxide (CO2) emissions. Further, the process energy demand and the energy recovery are also taken into account. The main objective of this paper is to analyze the key factors and sources of uncertainty influencing GHG emissions from WWTP at a pla…

Sensitivity and uncertainty analysis of an integrated ASM2d MBR model for wastewater treatment

Abstract An integrated membrane bioreactor (MBR) model was previously proposed and tested. The model provides a comprehensive and detailed description of the nitrogen biological removal processes with respect to up-to-date literature. This paper presents a sensitivity and uncertainty analysis aimed at identifying the key factors affecting the variability of the model predictions. The Standardized Regression Coefficients (SRC) method was adopted for the sensitivity analysis. The uncertainty analysis was employed by running Monte Carlo simulations by varying only the value of the key factors affecting the model outputs. The sensitivity analysis combined with the uncertainty analysis applied h…

Global sensitivity analysis in environmental water quality modelling: Where do we stand?

Global sensitivity analysis (GSA) is a valuable tool to support the use of mathematical models for environmental systems. During the last years the water quality modelling field has embraced the use of GSA. Environmental water quality modellers have tried to transfer the knowledge and experience acquired in other disciplines. The main objective of this paper is to provide an informed problem statement of the issues surrounding GSA applications in the environmental water quality modelling field. Specifically, this paper aims at identifying, for each GSA method, the potential use, the critical issues to be solved and the limits identified in a comprehensive literature review. The paper shows …

Variance-based sensitivity analysis for wastewater treatment plant modelling.

Global sensitivity analysis (GSA) is a valuable tool to support the use of mathematical models that characterise technical or natural systems. In the field of wastewater modelling, most of the recent applications of GSA use either regression-based methods, which require close to linear relationships between the model outputs and model factors, or screening methods, which only yield qualitative results. However, due to the characteristics of membrane bioreactors (MBR) (non-linear kinetics, complexity, etc.) there is an interest to adequately quantify the effects of non-linearity and interactions. This can be achieved with variance-based sensitivity analysis methods. In this paper, the Extend…

The influence of solid retention time on IFAS-MBR systems: analysis of system behavior.

A University of Cape Town Integrated Fixed-Film Activated Sludge Membrane Bioreactor (UCT-IFAS-MBR) pilot plant was operated at different values of the sludge retention time (SRT). Three SRTs were investigated at different durations: indefinitely, 30 and 15 days. The organic carbon, nitrogen and phosphorus removal, kinetic/stoichiometric parameters, membrane fouling tendency and sludge filtration properties were assessed. The findings showed that by decreasing the SRT, the pilot plant could maintain excellent carbon removal efficiencies throughout the experiments. In contrast, the biological carbon removal showed a slight nitrification and was slightly affected by the decrease of the SRT, s…

Biofouling management in anaerobic membrane bioreactors

Abstract This chapter is aimed at providing an insight on the main issues around the membrane fouling in anaerobic membrane bioreactors (AnMBRs). The analysis presented here shows that membrane fouling in AnMBRs is attributed to three major factors: membrane features, operating conditions, and wastewater characteristics. Several ad-hoc strategies have been implemented in literature to mitigate membrane fouling in AnMBRs, such as gas sparging, media addition, chemical cleaning, and membrane rotation/vibration. Among them, gas sparging is considered the most expensive strategy due to the high power requirement of the gas blower (up to 1.345 kWh m− 3). An accurate experimental and modeling ana…

Sequential batch membrane bio-reactor for wastewater treatment: The effect of increased salinity

In this work, a sequential batch membrane bioreactor pilot plant is investigated to analyze the effect of a gradual increase in salinity on carbon and nutrient removal, membrane fouling and biomass kinetic parameters. The salinity was increased by 2 g NaCl L-1 per week up to 10 g NaCl L-1. The total COD removal efficiency was quite high (93%) throughout the experiment. A gradual biomass acclimation to the salinity level was observed during the experiment, highlighting the good recovery capabilities of the system. Nitrification was also influenced by the increase in salinity, with a slight decrease in nitrification efficiency (the lowest value was obtained at 10 g NaCl L-1 due to lower nitri…

Comparison between two MBR pilot plants treating synthetic shipboard slops: effect of salinity increase on biological performance, biomass activity and fouling tendency

The paper reports the main results of an experimental campaign carried out on two bench scale pilot plants for the treatment of synthetic shipboard slops. In particular, two membrane bioreactors (MBRs) with submerged configuration were analyzed. One MBR pilot plant (namely, Line A) was fed with synthetic shipboard slop and was subjected to a gradual increase of salinity. Conversely, the second MBR pilot plant (namely, Line B) was fed with the same synthetic shipboard slop but without salt addition, therefore operating as a “control” unit. Organic carbon, hydrocarbons and ammonium removal, kinetic constants, extracellular polymeric substances (EPSs) production and membranes fouling rates…

The fouling phenomenon in membrane bioreactors: assessment of different strategies for energy saving

Membrane fouling represents one of the major limiting issue for membrane bioreactor (MBR) in the wastewater treatment field. Membrane fouling and high aeration requirement (for inducing shear stress to limit fouling) in MBR systems make the operation of such systems economically demanding due to high energetic costs. Despite several studies on MBR fouling have been performed a comprehensive knowledge on how to reduce membrane fouling and consequently energy saving is still lacking and controversial. The aim of this study is to gain insights on the optimization of the operating conditions in an MBR system. In particular, the influence of the aeration intensity and the durations of filtration…

A plant-wide modelling comparison between membrane bioreactors and conventional activated sludge

Abstract A comprehensive plant-wide mathematical modelling comparison between conventional activated sludge (CAS) and Membrane bioreactor (MBR) systems is presented. The main aim of this study is to highlight the key features of CAS and MBR in order to provide a guide for an effective plant operation. A scenario analysis was performed to investigate the influence on direct and indirect greenhouse gas (GHG) emissions and operating costs of (i) the composition of inflow wastewater (scenario 1), (ii) operating conditions (scenario 2) and (iii) oxygen transfer efficiency (scenario 3). Scenarios show higher indirect GHG emissions for MBR than CAS, which result is related to the higher energy con…

Global sensitivity analysis for urban water quality modelling: comparison of different methods

Sensitivity analysis represents an important step in improving the understanding and use of environmental models. Indeed, by means of global sensitivity analysis (GSA), modellers may identify both important (factor prioritization) and non-influential (factor fixing) model input factors. However, despite the potentialities of GSA methods, only few applications have been published in the field of urban drainage modelling. In order to fill this gap this paper presents a comparison among three GSA methods (SRC, Extended-FAST and Morris screening) on an urban drainage storm-water model. In particular, an exhaustive discussion on their peculiarities, applicability, and reliability is presented. S…

Membrane bioreactors for treatment of saline wastewater contaminated by hydrocarbons (diesel fuel): An experimental pilot plant case study

Abstract The paper reports the main results of an experimental campaign performed on a membrane bioreactor pilot plant designed to treat synthetic shipboard slops. The experimental campaign was divided into two phases: salinity acclimation up to 20 g NaCl L −1 (Phase I) and hydrocarbon (diesel fuel) dosing (Phase II). The observed results show that the carbon removal was not severely affected by the wastewater features. Conversely, respirometric tests showed that nitrification was strongly affected by the salinity (33% of nitrification efficiency at 20 g NaCl L −1 – Phase I) as a result of the salinity in the autotrophic biomass. Moreover, the sludge viscosity increased during Phase II due …

A Graphical User Interface as a DSS Tool for GHG Emission Estimation from Water Resource Recovery Facilities

A Grafical User Interface (GUI) for the greenhouse gas (GHG) emissions from WWTPs based on four models aimed at quantifying the gas emissions from the aerated tanks (i.e. CAS and MBR reactor), aerobic digesters, secondary clarifiers and anaerobic digesters have been englobed in a GUI in order to provide a valid decision support system (DSS) to the practitioners. The GUI allows to estimate such emissions for the different WWTP phases considered. The GUI has been developed on MATLAB platform and provides as output the GHG emissions in terms of CO2 and N2O fluxes.

The effect of hydrocarbon on a pilot plant membrane bioreactor system

The paper reports the main results from an experimental gathering campaign carried out on a bench scale plant for the evaluation of hydrocarbon effect on the system performance. In particular, a membrane bioreactor (MBR) under submerged configuration was analysed. The MBR plant was fed with synthetic wastewater containing hydrocarbons. Organic carbon, hydrocarbons and ammonium removal, kinetic constants, extracellular polymeric substances (EPSs) production and membranes fouling rates have been assessed. The observed results highlighted good system performance in terms of both COD removal and nitrification, thus showing a sort of biomass adaptation to hydrocarbon. Such a result has been also…

INCIDENZA SULL’ANALISI DI RISCHIO DELLE MODALITÁ DI MISURA DEI SOIL GAS: IL CASO DI UN S.I.N. SICILIANO

Il monitoraggio delle matrici aeriformi sta assumendo importanza crescente nell’ambito dei procedimenti di bonifica di siti contaminati, con riferimento sia all’esecuzione delle analisi di rischio, sia alla conseguente progettazione degli interventi di risanamento. L’utilizzo di misure dirette di campo si rivela di notevole interesse per la verifica di quelle indirette, ottenute con gli algoritmi implementati nei modelli matematici utilizzati per l’analisi di rischio. Obiettivo del lavoro presentato nella nota è stato quello di valutare l’influenza di misure dirette di soil gas e di flussi emissivi sull’analisi di rischio sanitaria. Come caso studio, si è considerato un S.I.N. nel territori…

UCT-MBR vs IFAS-UCT-MBR for Wastewater Treatment: A Comprehensive Comparison Including N2O Emission

In this study the performance (in terms of carbon and nutrient removal) and N2O emission of two plant configurations adopting innovative technologies were investigated. With this regards, an University Cape Town (UCT) membrane bioreactor (MBR) plant and an Integrated Fixed Film Activated Sludge (IFAS) -UCT-MBR plant were monitored. Both plants treat real wastewater under two different values of the influent carbon nitrogen ratio (C/N = 5 mgCOD/mgN and C/N = 10 mgCOD/mgN). Results have shown the highest carbon and nutrients removal efficiencies for the IFAS-UCT-MBR configuration during both the two investigated C/N values. Furthermore, the lowest N2O emission occurred for the IFAS-UCT-MBR.

Influence of carbon to nitrogen ratio on nitrous oxide emission in an Integrated Fixed Film Activated Sludge Membrane BioReactor plant

Abstract In this study a University of Cape Town (UCT) Integrated Fixed Film Activated Sludge (IFAS) Membrane BioReactor (MBR) wastewater treatment plant was monitored in terms of nitrous oxide (N2O) emissions. The short term effect on the N2O emission due to the influent carbon-to-nitrogen (C/N) ratio variation (C/N ratios of 2, 5 and 10 gCOD/gN) was evaluated. Since in a previous study, the effect of the C/N ratio was studied in the same system without biofilm (UCT-MBR configuration) the main aim here was to investigate the role of biofilms on N2O emissions. Under all the investigated C/N ratios, the N2O fluxes and the average emission factors were lower than that of previous studies with…

Sensitivity and uncertainty analysis of an integrated membrane bioreactor model

Sensitivity and uncertainty analysis, although can be of primarily importance in mathematical modelling approaches, are scarcely applied in the field of membrane bioreactor (MBR). An integrated mathematical model for MBR is applied with the final aim to pin down sources of uncertainty in MBR modelling. The uncertainty analysis has been performed combining global sensitivity analysis (GSA) with the generalized likelihood uncertainty estimation (GLUE). The model and methodology were applied to a University Cape Town pilot plant. Results show that the complexity of the modelled processes and the propagation effect from the influent to the effluent increase the uncertainty of the model predicti…

Nitrous oxide emission from a moving bed membrane biofilm reactor: the effect of the sludge retention time

The aim of the present study was to investigate the nitrous oxide (N2O) emissions from a University of Cape Town (UCT) moving bed membrane bioreactor pilot plant. An experimental campaign was carried out during 60 days with three different sludge retention time (SRT). The pilot plant reactor was provided of funnel shape covers that guaranteed gas accumulation in the headspace. The results highlighted that N2O concentrations significantly increased when the biofilm concentrations increased within the aerobic and anoxic compartments. Furthermore, results have shown an increase of N2O with the decrease of SRT. Moreover, the MBR tank resulted the key emission source (up to 70% of the total N2O …

The influence of solid retention time on IFAS-MBR systems: Assessment of nitrous oxide emission

The aim of the present study was to investigate the nitrous oxide (N2O) emissions from a moving bed based Integrated Fixed Film Activated Sludge (IFAS) - membrane bioreactor (MBR) pilot plant, designed according to the University of Cape Town (UCT) layout. The experimental campaign had a duration of 110 days and was characterized by three different sludge retention time (SRT) values (∞, 30 d and 15 d). Results highlighted that N2O concentrations decreased when the biofilm concentrations increased within the aerobic reactor. Results have shown an increase of N2O with the decrease of SRT. Specifically, an increase of N2O-N emission factor occurred with the decrease of the SRT (0.13%, 0.21% …

Integrated Fixed Film Activated Sludge (IFAS) membrane BioReactor: The influence of the operational parameters

Abstract The present paper investigated an Integrated Fixed Film Activated Sludge (IFAS) Membrane BioReactor (MBR) system monitored for 340 days. In particular, the short-term effects of some operational parameters variation was evaluated. Results showed a decrease of the removal rates under low C/N values. Respirometry results highlighted that activated sludge was more active in the organic carbon removal. Conversely, biofilm has a key role during nitrification. The major fouling mechanism was represented by the cake deposition (irreversible).

Il destino dei microinquinanti negli impianti di depurazione e nei corpi idrici.

• Monitoraggio di droghe in quattro impianti di depurazione siciliani e in un corpo idrico ricettore. • Elevata presenza nei reflui influenti a tutti gli impianti monitorati di THC-COOH e BEG, metaboliti rispettivamente della cannabis e della cocaina. • Fenomeni di degradazione naturale ad opera del biota e/o di accumulo nei sedimenti del corpo idrico ricettore comportano la riduzione delle concentrazione di THC-COOH.

Decision support systems (DSS) for wastewater treatment plants - A review of the state of the art.

The use of decision support systems (DSS) allows integrating all the issues related with sustainable development in view of providing a useful support to solve multi-scenario problems. In this work an extensive review on the DSSs applied to wastewater treatment plants (WWTPs) is presented. The main aim of the work is to provide an updated compendium on DSSs in view of supporting researchers and engineers on the selection of the most suitable method to address their management/operation/design problems. Results showed that DSSs were mostly used as a comprehensive tool that is capable of integrating several data and a multi-criteria perspective in order to provide more reliable results. Only …

High salinity wastewater treatment by membrane bioreactors

Abstract This chapter reviews the state of the art regarding the use of membrane bioreactor (MBR) systems for saline wastewater treatment. In particular, a comprehensive review is presented discussing the most adopted MBR configurations for the treatment of saline wastewater. Further, the chapter discusses the main effects of salinity on the biological performance, kinetic parameters, fouling development, sludge rheological features, and greenhouse gas (GHG) production. Literature results show that there is a significant effect of the salinity (and/or salinity variations) on the biological performance and membrane filtration. Specifically, it was found that a gradual salinity increase, carr…

The fouling phenomenon in membrane bioreactors: Assessment of different strategies for energy saving

Abstract Membrane fouling represents one of the major issues for a membrane bioreactor (MBR). Membrane fouling and high aeration requirements (for inducing shear stress to limit fouling) make MBR operation economically demanding due to high energy costs. Although several studies on MBR fouling have been performed, comprehensive knowledge on how to reduce membrane fouling and consequently save energy is still lacking. An integrated mathematical model for MBR is applied to a University of Cape Town membrane bioreactor with the final aim to reduce the energy costs. In particular, the influence of the aeration intensity, the duration of filtration/backwashing cycles, and the number of membrane …

Nitrous oxide from moving bed based integrated fixed film activated sludge membrane bioreactors

Abstract The present paper reports the results of a nitrous oxide (N 2 O) production investigation in a moving bed based integrated fixed film activated sludge (IFAS) membrane bioreactor (MBR) pilot plant designed in accordance with the University of Cape Town layout for biological phosphorous removal. Gaseous and liquid samples were collected in order to measure the gaseous as well as the dissolved concentration of N 2 O. Furthermore, the gas flow rate from each reactor was measured and the gas flux was estimated. The results confirmed that the anoxic reactor represents the main source of nitrous oxide production. A significant production of N 2 O was, however, also found in the anaerobic …

Toward a New Plant-Wide Experimental and Modeling Approach for Reduction of Greenhouse Gas Emission from Wastewater Treatment Plants

Mechanisms causing greenhouse gas (GHG) emission in wastewater treatment plants are of great interest among researchers, encouraging the development of new methods for wastewater management. Wastewater treatment plants (WWTPs) emit three major greenhouse gases during the treatment processes: CO2, CH4, and N2O. Additional amounts of CO2 and CH4 are produced during energy consumption, which can be considered an indirect source of GHGs. Recently, several efforts have been undertaken to assess GHGs from WWTPs, with particular attention paid to the N2O assessment due to its high warming potential (300 times stronger than CO2). This study proposes an integrated model platform for WWTP simulation,…

Illicit drugs consumption evaluation by wastewater-based epidemiology in the urban area of Palermo city (Italy)

Introduction. A wastewater-based epidemiology approach was performed to estimate the drug consumption in Palermo city, the fifth largest city of Italy with a population of 671 696 inhabitants, and to investigate the monthly variability of drug loads in wastewater from different areas of the city. A seven-months detection campaign was conducted at the two wastewater treatment plants of the city. Methods. Following a pre-treatment, 32 samples of wastewater were analyzed by liquid chromatography-tandem mass spectrometry. Results. We estimated a mean cocaine use in Palermo of 0.19 g/day/1000 people, corresponding to 1.90 doses/1000 people and cannabinoids use of 2.85 g/day/1000 people, correspo…

Global sensitivity analysis for urban water quality modelling: Terminology, convergence and comparison of different methods

Abstract Sensitivity analysis represents an important step in improving the understanding and use of environmental models. Indeed, by means of global sensitivity analysis (GSA), modellers may identify both important ( factor prioritisation ) and non-influential ( factor fixing ) model factors. No general rule has yet been defined for verifying the convergence of the GSA methods. In order to fill this gap this paper presents a convergence analysis of three widely used GSA methods (SRC, Extended FAST and Morris screening) for an urban drainage stormwater quality–quantity model. After the convergence was achieved the results of each method were compared. In particular, a discussion on peculiar…

THE USE OF MBR SYSTEMS FOR THE TREATMENT OF REAL SHIPBOARD SLOPS: ANALYSIS OF BIOLOGICAL PERFORMANCE AND BIOMASS BIOKINETIC ACTIVITY

In order to prevent hydrocarbon discharge at sea from ships, the International Maritime Organization (IMO) enacted the MARPOL 73/78 convention in which any oil and oil residue discharged in wastewater streams must contain less than 5 ppm hydrocarbons. Effective treatment of this petroleum-contaminated water is essential prior to its release into the environment, in order to prevent pollution problem for marine ecosystems as well as for human health. The present paper reports the first results of a field gathering campaign aimed at gaining insight about the treatment of real shipboard slops with a MBR pilot plant, in terms of pilot performance and biomass biokinetic activity. The achieved re…

Carbon and nutrient biological removal in a University of Cape Town membrane bioreactor: Analysis of a pilot plant operated under two different C/N ratios

Abstract The effect of the carbon-to-nitrogen (C/N) ratio variation in a University of Cape Town Membrane bioreactor (UCT-MBR) was investigated. The experimental campaign was divided into two phases, each characterized by a different C/N ratio (namely, 10 and 5, Phase I and Phase II, respectively). The UCT-MBR pilot plant was analysed in terms of carbon and nutrients removal, biomass respiratory activity, activated sludge features and membrane fouling. The results highlighted that the nutrients removal was significantly affected by the decrease of the C/N ratio during the Phase II. The biological carbon removal was also affected by the low C/N value during the Phase II. Indeed, the average …

Treatment of Oily Wastewater with Membrane Bioreactor Systems

The aim of the present work was to investigate the behavior of a membrane bioreactor (MBR) system for the treatment of oily wastewater. A bench scale MBR was fed with synthetic wastewater containing diesel fuel. Organic carbon, hydrocarbon and ammonium removal, kinetic constants, extracellular polymeric substances production, and membrane fouling rates were monitored. The MBR plant was operated for more than 200 days, and the results highlighted good carbon removal and nitrification, suggesting a sort of biomass adaptation to hydrocarbons. Membrane fouling analysis showed an increase in total resistance, likely due to hydrocarbons, which caused an irreversible fouling (pore blocking) mainly…

Initial pH Conditions Shape the Microbial Community Structure of Sewage Sludge in Batch Fermentations for the Improvement of Volatile Fatty Acid Production

Conversion of wastewater treatment plants into biorefineries is a sustainable alternative for obtaining valuable compounds, thus reducing pollutants and costs and protecting the environment and human health. Under specific operating conditions, microbial fermentative products of sewage sludge are volatile fatty acids (VFA) that can be precursors of polyhydroxyalkanoate thermoplastic polyesters. The role of various operating parameters in VFA production has yet to be elucidated. This study aimed to correlate the levels of VFA yields with prokaryotic microbiota structures of sewage sludge in two sets of batch fermentations with an initial pH of 8 and 10. The sewage sludge used to inoculate th…

Nitrous oxide from integrated fixed-film activated sludge membrane bioreactor: Assessing the influence of operational variables

The influence of the main operational variables on N2O emissions from an Integrated Fixed Film Activated Sludge University of Cape Town membrane Bioreactor pilot plant was studied. Nine operational cycles (total duration: 340 days) were investigated by varying the value of the mixed liquor sludge retention time (SRT) (Cycles 1–3), the feeding ratio between carbon and nitrogen (C/N) (Cycles 4–6) and simultaneously the hydraulic retention time (HRT) and the SRT (Cycles 7–9). Results show a huge variability of the N2O concentration in liquid and off-gas samples (ranged from 10−1μg N2O-N L−1to 103μg N2O-N L−1). The maximum N2O concentration (1228 μg N2O-N L−1) in the off-gas sa…

The GSA-GLUE approach for uncertainty assessment of an integrated MBR model

In the recent literature, the uncertainty of wastewater treatment plant (WWTP) modelling has had relevant interest both for designers and operators. Indeed, the frequent data lacking and the need to improve process knowledge, in case of innovative technologies, make the use of mathematical models more uncertain. Therefore, the need to make model uncertainty more explicit is warmly recommended. However, only few applications of uncertainty analysis in the wastewater field have been carried out. In this work, the combination of the global sensitivity analysis (GSA) and the Generalized Likelihood Uncertainty Estimation (GLUE) is applied for the uncertainty assessment of a WWTP model, in order …

Greenhouse Gas Emissions from Membrane Bioreactors

Nowadays, it is widely accepted that wastewater treatment plants (WWTPs) are significant sources of greenhouse gas (GHG) emission, contributing to the anthropogenic sources. Among the GHG emitted from WWTPs, nitrous oxide (N2O) has been identified of having the major interest/concern, since its high global warming potential (GWP), is 298 times higher than that of CO2 and also to its capability to react with stratospheric ozone causing the layer depletion. Up to now, most of the experimental investigations have been carried out on conventional activated sludge (CAS) processes. The knowledge of N2O emission from advanced technologies such membrane bioreactors (MBRs) is still very limited. The…

Solids and Hydraulic Retention Time Effect on N2O Emission from Moving-Bed Membrane Bioreactors

Biological nutrient removal was operated at different solids (SRT) and hydraulic retention times (HRT) in order to assess their influence on nitrous oxide (N2O) emission from a hybrid moving-bed membrane bioreactor. The observed results show that the N2O production decreased when the SRT/HRT was decreased. The maximum N2O gaseous concentration was measured in the aerobic reactor at the end of phase I, and it decreased through phases II and III. From mass balances over the reactors of the system, the aerated (aerobic and membrane) reactors were the largest producers of N2O, showing that the greater part of N2O was produced during the nitrification process.

Integrated fixed-film activated sludge membrane bioreactors versus membrane bioreactors for nutrient removal: A comprehensive comparison

Abstract This research elucidates the pollutants (nutrients and carbon) removal performance and nitrous oxide (N2O) emissions of two pilot plants. Specifically, a University of Cape Town (UCT) Membrane Bioreactor (MBR) plant and an Integrated Fixed Film Activated Sludge (IFAS)-UCT-MBR plant were investigated. The plants were fed with real wastewater augmented with acetate and glycerol in order to control the influent carbon nitrogen ratio (C/N). The short-term effect of the inlet C/N ratio variation (C/N = 5 mgCOD/mgN and C/N = 10 mgCOD/mgN) on the behaviour of both plants was investigated. The results showed that the IFAS-UCT-MBR configuration provided the best performance in terms of poll…

Towards a conceptual mathematical tool linking physical and biological processes for a reduction of ghg emissions from an mb-mbr plant

The current study explores the influence of the air flow rate on greenhouse gas (GHG) emissions (direct and indirect), the operational costs (OCs), the effluent quality index (EQI) and effluent fines (EF). An University Cape Town (UCT) moving bed (MB) membrane bioreactor (MBR) pilot plant has been considered as case study where the influence of the air flow rate on the biological and physical processes has been analyzed. Constitutive relationships between the air flow rate and some performance indicators (i.e., EQI, OCs, direct and indirect GHG emissions) have been identified. Results showed that the EQI increases at low flow rate likely due to the dissolved oxygen (DO) limitation in the bi…

Nitrous oxide emission in a University of Cape Town membrane bioreactor: The effect of carbon to nitrogen ratio

Abstract The effect of the carbon-to-nitrogen (C/N) ratio in the influent on the nitrous oxide (N 2 O) emission from a University of Cape Town Membrane BioReactor pilot plant was investigated. The membrane was located in a separate tank to single out the production of N 2 O due to the biological processes from N 2 O stripping as a result of the extra aeration needed for the mitigation of membrane fouling. The experimental campaign was divided into two phases, each characterized by a different C/N ratio (namely, 10 and 5 mgCOD/mgTN, Phase I and Phase II, respectively). The decrease of the C/N ratio promoted the increase of N 2 O emissions in both gaseous and dissolved phases, mainly due to a…

Greenhouse gas emissions and the links to plant performance in a fixed-film activated sludge membrane bioreactor - Pilot plant experimental evidence

The present study explores the interlinkages among the operational variables of a University of Cape Town (UCT) Integrated Fixed Film Activated Sludge (IFAS) membrane bioreactor (MBR) pilot plant. Specifically, dedicated experimental tests were carried out with the final aim to find-out a constitutive relationship among operational costs (OCs), effluent quality index (EQI), effluent fines (EF). Greenhouse gas (GHG) emissions were also included in the study. Results showed that the EQI increases at low flow rate likely due to the dissolved oxygen (DO) limitation in the biological processes. Direct GHGs increase with the increasing of the air flow due to the anoxic N2O contribution. Irreversi…

Global sensitivity analysis in wastewater applications: A comprehensive comparison of different methods

Three global sensitivity analysis (GSA) methods are applied and compared to assess the most relevant processes occurring in wastewater treatment systems. In particular, the Standardised Regression Coefficients, Morris Screening and Extended-FAST methods are applied to a complex integrated membrane bioreactor (MBR) model considering 21 model outputs and 79 model factors. The three methods are applied with numerical settings as suggested in literature. The main objective considered is to classify important factors (factors prioritisation) as well as non-influential factors (factors fixing). The performance is assessed by comparing the most reliable method (Extended-FAST), by means of proposed…

A novel comprehensive procedure for estimating greenhouse gas emissions from water resource recovery facilities

The emissions of the major greenhouse gases (GHGs), i.e. carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) from water resource recovery facilities (WRRFs) are of increasing concern in the water industry. In order to produce useful and comparable information for monitoring, assessing, and reporting GHG emissions from WRRFs, there is a need for a generally accepted methodology for their quantification. This paper aims at proposing the first protocol for monitoring and accounting for GHG emissions from WRRFs, taking into account both direct and indirect internal emissions and focusing the attention on plant sections known to be primarily responsible for GHG emissions (i.e. oxidation…

Il destino dei microinquinanti negli impianti di depurazione

La problematica dell’inquinamento prodotta dai microinquinanti, cioè da sostanze presenti in tracce nei corpi ricettori, ma aventi caratteristiche tali da comportare possibili danni per l’ambiente e per l’uomo, ha assunto un’importanza sempre crescente nell’ultimo ventennio. Infatti essa è ormai oggetto di diversi interventi normativi a livello comunitario e nazionale, che hanno individuato un elenco di sostanze definire “prioritarie” e “pericolose prioritarie”, di cui sono state fissate le concentrazioni massime per i corpi ricettori al fine di garantire voluti livelli di qualità ambientale; a queste si aggiunge un elenco molto numeroso di “contaminanti emergenti”, non ancora oggetto di no…

Greenhouse gases from membrane bioreactors: a pilot plant case study

In this study the N2O emitted from the aerobic and anoxic tank of a Sequential Batch (SB) Membrane Bioreactor (MBR) pilot plant has been investigated. The SB-MBR pilot plant was fed with a mixture of synthetic and real wastewater. The influence of salinity on N2O concentration was analyzed by gradually increasing the salinity by addition of salt to concentrations ranging from 0 to 10 gNaCl L-1. The results demonstrated that the N2O concentration of the gaseous samples was strongly influenced by the salinity in the range of 0.25 ppm to 3.4 ppm salinity. This result was explained by lower autotrophic kinetics due to the biomass lysis. Dissolved oxygen concentration and salinity were found to …

Greenhouse gases from wastewater treatment plant: towards a new protocol for field measurements

Greenhouse gases (GHGs) emission from wastewater treatment plants (WWTPs) represents an increasing interest in the water industry at the present moment. The biological processes needed for treating wastewater have been found responsible for the unintentional generation of GHGs, such as carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O). The quality and quantity of a GHG emitted from a WWTP can significantly vary with the wastewater treated, the process configuration, and the strategies employed in process control. In the recent past, efforts for monitoring and accounting for GHGs emissions from WWTPs have considerably increased, and classifications of emission type were proposed t…

Global sensitivity analysis in ASM applications: comparison of the SRC and Extended-FAST method for a UCT-MBR model

In this study global sensitivity analysis is performed to identify influential as well as non-influential parameters in a model of a University Cape Town Membrane Bioreactor (UCT-MBR). In particular, the Standardised Regression Coefficients (SRC) and Extended-FAST sensitivity analysis methods are applied. The sensitivity of model variables towards parameter variation is analysed for CODTOT, SNH4, SNO3, SPO, and MLSS along five reactor compartments. Both methods indicate that the parameters identified as being influential differ from section to section due to the different processes involved. Moreover, the relevant influence of the membrane filtration parameters is detected in the first plan…

Variance-based sensitivity analysis for wastewater treatment plant modelling

Global sensitivity analysis (GSA) is a valuable tool to support the use of mathematical models that characterise technical or natural systems. In the field of wastewater modelling, most of the recent applications of GSA use either regression-based methods, which require close to linear relationships between the model outputs and model factors, or screening methods, which only yield qualitative results. However, due to the characteristics of membrane bioreactors (MBR) (non-linear kinetics, complexity, etc.) there is an interest to adequately quantify the effects of non-linearity and interactions. This can be achieved with variance-based sensitivity analysis methods. In this paper, the Extend…

Aeration control in membrane bioreactor for sustainable environmental footprint

In this study different scenarios were scrutinized to minimize the energy consumption of a membrane bioreactor system for wastewater treatment. Open-loop and closed-loop scenarios were investigated by two-step cascade control strategies based on dissolved oxygen, ammonia and nitrite concentrations. An integrated MBR model which includes also the greenhouse gas formation/emission processes was applied. A substantial energy consumption reduction was obtained for the closed-loop scenarios (32% for Scenario 1 and 82% for Scenario 2). The air flow control based on both ammonia and nitrite concentrations within the aerobic reactor (Scenario 2) provided excellent results in terms of reduction of o…

Sewage sludge acidogenic fermentation for organic resource recovery towards carbon neutrality: an experimental survey testing the headspace influence

Volatile fatty acids (VFAs) produced by acidogenic digestion of sewage sludge are very interesting bio-products which can contribute to carbon neutrality of wastewater treatment plants. Studies on the production of VFAs from sewage sludge from fermenters with membrane are limited. In view of above, VFAs from a fermenter pilot plant equipped with a membrane bioreactor and fed with real sewage sludge has been monitored. The effect of headspace volume (HdV) on VFA production was studied for the first time to elucidate the optimal operation conditions. Specifically, three fermenter HdV values (namely, 20, 40 and 60% of the total volume) have been investigated. Results revealed that the HdV of 2…

A model for biological nitrogen and phosphorus removal: application to a full scale WWTP Proc. - Firenze 24-27/06/2008

Roadmapping the Transition to Water Resource Recovery Facilities: The Two Demonstration Case Studies of Corleone and Marineo (Italy)

The current exploitation of freshwater, as well as the significant increase in sewage sludge production from wastewater treatment plants (WWTPs), represent nowadays a critical issue for the implementation of sustainable development consistent with the circular economy concept. There is an urgent need to rethink the concept of WWTPs from the conventional approach consisting in pollutant removal plants to water resource recovery facilities (WRRFs). The aim of this paper is to provide an overview of the demonstration case studies at the Marineo and Corleone WRRFs in Sicily (IT), with the final aim showing the effectiveness of the resources recovery systems, as well as the importance of plant o…

Uncertainty assessment of a model for biological nitrogen and phosphorus removal: Application to a large wastewater treatment plant

Abstract In the last few years, the use of mathematical models in WasteWater Treatment Plant (WWTP) processes has become a common way to predict WWTP behaviour. However, mathematical models generally demand advanced input for their implementation that must be evaluated by an extensive data-gathering campaign, which cannot always be carried out. This fact, together with the intrinsic complexity of the model structure, leads to model results that may be very uncertain. Quantification of the uncertainty is imperative. However, despite the importance of uncertainty quantification, only few studies have been carried out in the wastewater treatment field, and those studies only included a few of …

Characterization of wastewater and biomass activity in a membrane bioreactor using respirometric techniques

Over the last two decades, Membrane Bioreactors (MBRs) emerged even more for wastewater treatment, ensuring high removal efficiencies as well as very small footprint required. Indeed, in this kind of process, a modification in biomass activity and viability can exist compared to that of a conventional activated sludge (CAS) process. In this context, respirometric analysis represents a reliable tool in order to evaluate the actual biomass kinetic parameters, to insert in mathematical models in the design phase, as well as to monitor the biomass viability, especially when these processes are operated with high sludge retention time (SRT) values. The paper presents some results of respirometri…

Water Resource Recovery Facilities (WRRFs): The Case Study of Palermo University (Italy)

The wastewater sector paradigm is shifting from wastewater treatment to resource recovery. In addition, concerns regarding sustainability during the operation have increased. In this sense, there is a need to break barriers (i.e., social, economic, technological, legal, etc.) for moving forward towards water resource recovery facilities and demonstration case studies can be very effective and insightful. This paper presents a new water resource recovery case study which is part of the Horizon 2020 EU Project “Achieving wider uptake of water-smart solutions—Wider Uptake”. The final aim is to demonstrate the importance of a resource recovery system based on the circular economy concept. The r…

Global sensitivity analysis for micropollutant modeling by means of an urban integrated approach

The paper presents the sensitivity analysis of an integrated urban water quality system by means of the global sensitivity analysis (GSA). Specifically, an home-made integrated model developed in previous studies has been modified in order to include the micropollutant assessment (namely, sulfamethoxazole - SMX). The model is able to estimate also the interactions between the three components of the system: sewer system (SS), wastewater treatment plant (WWTP) and the receiving water body (RWB). The analysis has been applied to an experimental catchment nearby Palermo (Italy): the Nocella catchment. Five scenarios each characterized by different combinations of sub-systems (i.e., SS, WWTP an…

Bacterial community structure and removal performances in IFAS-MBRs: A pilot plant case study

Abstract The paper reports the results of an experimental campaign carried out on a University of Cape Town (UCT) integrated fixed-film activated sludge (IFAS) membrane bioreactor (MBR) pilot plant. The pilot plant was analysed in terms of chemical oxygen demand (COD) and nutrients removal, kinetic/stoichiometric parameters, membrane fouling and sludge dewaterability. Moreover, the cultivable bacterial community structure was also analysed. The pilot plant showed excellent COD removal efficiency throughout experiments, with average value higher than 98%, despite the slight variations of the influent wastewater. The achieved nitrification efficiency was close to 98% for most of the experimen…

Greenhouse gases from membrane bioreactors: New perspectives on monitoring and mathematical modeling

Abstract The awareness that the wastewater sector represents a significant source of greenhouse gas emission has increased, and is the awareness is evident based on the numerous research papers published in technical literature in the last 10 years. This chapter is aimed at providing a timely overview on greenhouse gas production and emission from membrane bioreactors. Specifically, this chapter dedicates particular attention to nitrous oxide (N2O), considering both experimental and mathematical modeling approaches. Referring to the experimental data, the observed results highlighted the significant contribution of the aerated compartments toward nitrous oxide emission as well as the import…

Greenhouse gases from wastewater treatment — A review of modelling tools

Nitrous oxide, carbon dioxide and methane are greenhouse gases (GHG) emitted from wastewater treatment that contribute to its carbon footprint. As a result of the increasing awareness of GHG emissions from wastewater treatment plants (WWTPs), new modelling, design, and operational tools have been developed to address and reduce GHG emissions at the plant-wide scale and beyond. This paper reviews the state-of-the-art and the recently developed tools used to understand and manage GHG emissions from WWTPs, and discusses open problems and research gaps. The literature review reveals that knowledge on the processes related to N2O formation, especially due to autotrophic biomass, is still incompl…

Greenhouse gas emissions from membrane bioreactors: analysis of a two-year survey on different MBR configurations

Abstract This study aimed at evaluating the nitrous oxide (N2O) emissions from membrane bioreactors (MBRs) for wastewater treatment. The study investigated the N2O emissions considering multiple influential factors over a two-year period: (i) different MBR based process configurations; (ii) wastewater composition (municipal or industrial); (iii) operational conditions (i.e. sludge retention time, carbon-to-nitrogen ratio, C/N, hydraulic retention time); (iv) membrane modules. Among the overall analysed configurations, the highest N2O emission occurred from the aerated reactors. The treatment of industrial wastewater, contaminated with salt and hydrocarbons, provided the highest N2O emission…

A new plant wide modelling approach for the reduction of greenhouse Gas emission from wastewater treatment plants

Recent studies about greenhouse gas (GHG) emissions show that sewer collection systems and wastewater treatment plants (WWTPs) are anthropogenic GHG potential sources. Therefore, they contribute to the climate change and air pollution. This increasing interest towards climate change has led to the development of new tools for WWTP design and management. This paper presents the first results of a research project aiming at setting-up an innovative mathematical model platform for the design and management of WWTPs. More specifically, the study presents the project’s strategy aimed at setting-up a plant-wide mathematical model which can be used as a tool for reducing/controlling GHG from WWTP.…

Greenhouse gases from membrane bioreactors: Mathematical modelling, sensitivity and uncertainty analysis

In this study a new mathematical model to quantify greenhouse gas emissions (namely, carbon dioxide and nitrous oxide) from membrane bioreactors (MBRs) is presented. The model has been adopted to predict the key processes of a pilot plant with pre-denitrification MBR scheme, filled with domestic and saline wastewater. The model was calibrated by adopting an advanced protocol based on an extensive dataset. In terms of nitrous oxide, the results show that an important role is played by the half saturation coefficients related to nitrogen removal processes and the model factors affecting the oxygen transfer rate in the aerobic and MBR tanks. Uncertainty analysis showed that for the gaseous mod…

Insights on mechanisms of excess sludge minimization in an oxic-settling-anaerobic process under different operating conditions and plant configurations

In the present research, insights about the mechanisms of excess sludge minimization occurring in an oxic-settling-anaerobic (OSA) were provided. The investigation involved two systems operating in parallel. In particular, a conventional activated sludge (CAS) system as control and a system implementing the OSA process both having a pre-denitrification scheme were considered. Five periods (P1-P5) were studied, during which several operating conditions and configurations were tested. Specifically, the hydraulic retention time (HRT) in the anaerobic reactor of the OSA system (P1 8 h, P2-P3 12 h, P4 8 h, P5 12 h) and the return sludge from the anaerobic to the anoxic (scheme A) (P1-P2) or aero…

Evaluation of biomass activity and wastewater characterization in a UCT-MBR pilot plant by means of respirometric techniques

Abstract Over the last two decades, Membrane Bioreactors (MBRs) emerged even more for wastewater treatment, ensuring high removal efficiencies as well as very small footprint requirements. Indeed, in this kind of process, a modification in biomass activity and viability can exist compared to that of a CAS process. In this context, respirometric analysis represents a reliable tool in order to evaluate the actual biomass kinetic parameters, to insert in mathematical models in the design phase, as well as to monitor the biomass viability, especially when these processes are operated with high SRT values. The paper presents some results of respirometric techniques applied for the characterisati…