Differential accumulation of beta-1,3-glucanase and chitinase isoforms in tomato roots in response to colonization by either pathogenic or non-pathogenic strains of Fusarium oxysporum

International audience

Plasma membrane in arbuscular mycorrhiza

Protein profiling analyses in arbuscular mycorrhizal symbiosis

Cellular and subcellular studies of the arbuscular mycorrhizal symbiosis in M. truncatula: a proteomic survey

A Medicago truncatula root plasma membrane enriched fraction as a prerequisite for label free quantitative analysis of plasmalemma protein changes upon arbuscular mycorrhizal symbiosis

Proteome adaptations under contrasting soil phosphate regimes of Rhizophagus irregularis engaged in a common mycorrhizal network.

International audience; For many plants, their symbiosis with arbuscular mycorrhizal fungi plays a key role in the acquisition of mineral nutrients such as inorganic phosphate (Pi), in exchange for assimilated carbon. To study gene regulation and function in the symbiotic partners, we and others have used compartmented microcosms in which the extra-radical mycelium (ERM), responsible for mineral nutrient supply for the plants, was separated by fine nylon nets from the associated host roots and could be harvested and analysed in isolation. Here, we used such a model system to perform a quantitative comparative protein profiling of the ERM of Rhizophagus irregularis BEG75, forming a common my…

Analyse protéomique de l'impact de boues de stations d'épuration sur deux symbioses d'intérêt majeur en agriculture

Effets protecteurs de la symbiose mycorhizienne à arbuscule vis - à - vis du cadmium: identification des protéines potentiellement impliquées et quantification de deux transcrits

The arbuscular mycorrhizal transportome, what next!

International audience; Understanding how arbuscular mycorrhizal (AM) symbioses establish and function is one of the most important current challenges in microbial ecology. Despite the fact that the AM symbiosis requires some complex and fine molecular tuning among symbionts in order to take place, both partners benefit from each other in a number of ways. For instance, the availability, uptake and exchange of nutrients in this biotrophic interaction are key factors driving plant growth and modulating biomass allocation. This underground trade is regulated by both plant and fungal transport components [1], as for instance the long distance transport of photosynthates from leaves towards col…

Diversity and interactions among strains of Fusarium oxysporum : application to biological control.

International audience

Plant proteome analysis

Proteome analysis is becoming a powerful tool in the functional characterization of plants. Due to the availability of vast nucleotide sequence information and based on the progress achieved in sensitive and rapid protein identification by mass spectrometry, proteome approaches open up new perspectives to analyze the complex functions of model plants and crop species at different levels. In this review, an overview is given on proteome studies performed to analyze whole plants or specific tissues with particular emphasis on important physiological processes such as germination. The chapter on subcellular proteome analysis of plants focuses on the progress achieved for plastids and mitochond…

Studying the arbuscular mycorrhizal symbiosis from axenic system to soilborne interactions by proteomics: which challenges to face?

Membrane proteomic of arbuscular mycorrhiza

Fungal proteins in the extra-radical phase of arbuscular mycorrhiza: a shotgun proteomic picture

International audience

Membrane proteomic analyses to reveal and identify arbuscular mycorrhizal-related proteins

Proteomics: a functional genomic approach for studying mycorrhizal interactions in legumes

Variabilité quantitative des carboydrates de surface des conidies de Fusarium oxysporum

Mutations in DMI3 and SUNN modify the appressorium-responsive root proteome in arbuscular mycorrhiza.

Modification of the Medicago truncatula root proteome during the early stage of arbuscular mycorrhizal symbiosis was investigated by comparing, using two-dimensional electrophoresis, the protein patterns obtained from non-inoculated roots and roots synchronized for Glomus intraradices appressorium formation. This approach was conducted in wild-type (J5), mycorrhiza-defective (TRV25, dmi3), and autoregulation-defective (TR122, sunn) M. truncatula genotypes. The groups of proteins that responded to appressorium formation were further compared between wild-type and mutant genotypes; few overlaps and major differences were recorded, demonstrating that mutations in DMI3 and SUNN modified the ap…

Analyse protéique de différentes souches de Fusarium oxysporum

The membrane proteome of Medicago truncatula roots displays qualitative and quantitative changes in response to arbuscular mycorrhizal symbiosis

International audience; Arbuscular mycorrhizal (AM) symbiosis that associates roots of most land plants with soil-borne fungi (Glomeromycota), is characterized by reciprocal nutritional benefits. Fungal colonization of plant roots induces massive changes in cortical cells where the fungus differentiates an arbuscule, which drives proliferation of the plasma membrane. Despite the recognized importance of membrane proteins in sustaining AM symbiosis, the root microsomal proteome elicited upon mycorrhiza still remains to be explored. In this study, we first examined the qualitative composition of the root membrane proteome of Medicago truncatula after microsome enrichment and subsequent in dep…

Organelle protein changes in arbuscular mycorrhizal Medicago truncatula roots as deciphered by subcellular proteomics

Prod 2020-8c SPE IPM INRA UB CNRS; The roots of most land plants can enter a symbiotic relationship with arbuscular mycorrhizal (AM) soil‐borne fungi belonging to the phylum Glomeromycota, which improves the mineral nutrition of the host plant. The fungus enters the root through the epidermis and grows into the cortex where it differentiates into a highly branched hyphal structure called the arbuscule. The role of the plant membrane system as the agent for cellular morphogenesis and signal/nutrient exchanges is especially accentuated during AM endosymbiosis. Notably, fungal hyphae are always surrounded by the host membrane, which is referred to as the perifungal membrane around intracellula…

Subcellular proteomics sheds light on root plastid involvement in Medicago truncatula arbuscular mycorrhizal symbiosis

Technical improvements for analysis of récalcitrant proteins by LC-MS

Proteomics of different tissues

Livre publié sous forme électronique; International audience

Protéome membranaire en réponse à la symbiose mycorhizienne à arbuscules par GeLC-MS/MS

Characterization of the "viable but nonculturable" (VBNC) state in the wine spoilage yeast Brettanomyces.

Although the viable but not culturable (VBNC) state has been studied in detail in bacteria, it has been suggested that maintenance of viability with loss of culturability also exists in eukaryotic cells, such as in the wine spoilage yeast Brettanomyces. To provide conclusive evidence for the existence of a VBNC state in this yeast, we investigated its capacity to become viable and nonculturable after sulfite stress, and its ability to recover culturability after stressor removal. Sulfite addition induced loss of culturability but maintenance of viability. Increasing the medium pH to decrease the concentration of toxic SO(2) allowed yeast cells to become culturable again, thus demonstrating …

La tolérance au cadmium conférée par la symbiose endomycorhizienne s’accompagne de remaniements protéiques dans les organes aériens

Impact of phosphate fertilization and arbuscular mycorrhiza on the development, nutrition and quality of micropropagated walnut rootstocks.

The English walnut (Juglans regia L.) is the main species cultivated for the production of edible nuts. Due to heterozygosis, the characteristics of agronomical interest of walnut cultivars are not inherited via seed propagation. In vitro plant tissue culture thus plays a key role in mass propagation of high-quality walnut rootstocks. Micropropagation of walnut explants needs an ex vitro acclimatization phase to repair the in vitro induced abnormalities, and further requires a post-acclimatization growth in greenhouse conditions when plantlets become photoautotrophic. However, poor survival and slow growth rates are common difficulties encountered in nurseries when establishing micropropaga…

Plant beneficiating from arbuscular mycorrhizal symbiosis: impact of fungal diversity and soil fertilization

Membrane proteomics of arbuscular mycorrhiza

International audience

Proteomic approaches to learn more on legume-micobe interactions

Vers la définition d’un programme symbiotique régissant les interactions mycorhiziennes

Recovery of Fusarium oxysporum Fo47 mutants affected in their biocontrol activity after transposition of the Fot1 element

Trouvelot, S., Olivain, C., Recorbet, G., Migheli, Q., and Alabouvette, C. 2002. Recovery of Fusarium oxysporum Fo47 mutants affected in their biocontrol activity after transposition of the Fot1 element. Phytopathology 92:936-945. To investigate the biocontrol mechanisms by which the antagonistic Fusarium oxysporum strain Fo47 is active against Fusarium wilt, a Fot1 transposon-mediated insertional mutagenesis approach was adopted to generate mutants affected in their antagonistic activity. Ninety strains in which an active Fot1 copy had transposed were identified with a phenotypic assay for excision and tested for their biocontrol activity against F. oxysporum f. sp. lini on flax in greenho…

Production and characterization of pathogenicy-deficient mutants of Fusarium oxysporum f. sp. melonis

Mycorrhization-induced changes in the root plastid proteome of Medicago truncatula

Rapport de la session The future landscape of arbuscular mycorrhiza research. COST Action 8.38 Dijon

La tolérance des plantes mycorhizées au cadmium : mécanismes impliqués. Contrat Région Bourgogne CRB : 06 516 CP O15 S 248

A proteomic approach to study the effects of two polycyclic aromatic hydrocarbons on arbuscular mycorrhiza of Cichorium intybus roots in gnotobiotic conditions

Cadmium stress alleviation by arbuscular mycorrhizal symbiosis: proteomic studies of the mechanisms involved in shoots of M. truncatula

Proteomes of arbuscular mycorrhizal symbiosis : past and present achievements

Label-free 1-DE-LC-MS/MS and iTRAQ-OFFGEL-LC-MS/MS to identify arbuscular mycorrhiza-related membrane proteins.

Exploring the Genome of glomeromycotan fungi

Chapitre 1; International audience; All fungi forming a mutualistic symbiosis with plant roots called arbuscular mycorrhiza were formerly grouped together in one order, the Glomales, placed in the Zygomycota (Morton 1993). Based on molecular analyses suggestingthat arbuscular mycorrhizal fungi should be separated from other fungal taxa, they were transferred a decade ago to the Glomeromy-cota , a new phylum created specifically for them (Schu¨ ssler et al. 2001). Whilst members of this monophyletic group originated from the same common ancestor as the Ascomycota and Basidiomycota, they have no obvious affinity to other major extant phylogenetic groups in the kingdom Fungi (James et al. 2006) …

Plant mycorrhizal interaction: What can we learn from a proteomic approach?

Participation au « Model Legumes Congress 2007 » (MLC2007)- Compte-rendu de mission

Wanted : pathogenesis-related marker molecules for Fusarium oxysporum

Summary Although Fusarium oxysporum pathogens cause severe wilts in about 80 botanical species, the mechanisms of pathogenicity and symptom induction are poorly understood. Knowledge about the genetic and biochemical pathways involved in the pathogenesis of F. oxysporum would be invaluable in getting targets for both fungicide development and search for biocontrol agents. In this respect, we described the main approaches that have been developed to identify some mechanisms underlying the pathogenesis of F. oxysporum . During the last decades, the potential functions triggering of F. oysporum pathogenicity have mainly been investigated by comparing soilborne pathogenic strains with nonpathog…

Functional analysis of the membrane proteome of Medicago truncatula roots upon colonization by the arbuscular mycorrhizal fungus Glomus irregulare

International audience

Protein actors sustaining arbuscular mycorrhizal symbiosis: underground artists break the silence

'Summary' 26 I. 'Casting for a scenario' 26 II. 'Nominees for a preliminary role' 27 III. 'Nominees for a leading role' 32 IV. 'Future artists' 37   'Acknowledgements' 38   References 38 Summary The roots of most land plants can enter a relationship with soil-borne fungi belonging to the phylum Glomeromycota. This symbiosis with arbuscular mycorrhizal (AM) fungi belongs to the so-called biotrophic interactions, involving the intracellular accommodation of a microorganism by a living plant cell without causing the death of the host. Although profiling technologies have generated an increasing depository of plant and fungal proteins eligible for sustaining AM accommodation and functioning, a …

Un repertoire GeLC - MS/MS des proteins extra - radiculaires de Glomus intraradices

Arbuscular mycorhizal proteomes: what news at the nearby and distant horizon?

International audience; Proteomics has soon emerged as a powerful tool to point out protein modifications in roots interacting with arbuscular mycorrhiza (AM) fungi. Depending on the developmental mycorrhizal stage and on the available amount of mycorrhizal material, untargeted and/or sub-cellular proteomic approaches were applied to reveal and identify proteins whose accumulation was modified during the AM colonisation of Medicago truncatula roots. For the early stage of symbiosis, the protein patterns obtained from noninoculated roots and roots synchronized for appressorium formation in wild-type (Jemalong J5), penetration-defective (TRV25, dmi3) and autoregulation-defective (TR122, sunn)…

Fungal protein accumulation in the extra - radical phase of arbuscular mycorrhiza: a shotgun proteomic picture

Un répertoire GeLC - MS/MS des protéines extra - radiculaires de Glomus intraradices

Contribution of proteomics to arbuscular mycorrhiza in Medicago truncatula

International audience; Because proteins are key effectors of plant responses to environmental cues including recognition, signalling, transport and defence reactions, main interest has been paid to characterize those involved in the establishment and functioning of arbuscular mycorrhizal (AM) symbiosis. In our group, the setting up of high throughput proteomic techniques on the model species, Medicago truncatula, is providing step-by-step a large-scale analysis of AM symbiosis-related proteins. Depending on the symbiotic stage targeted and on the abundance of mycorrhizal material, different proteomic strategies that can be combined with other large-scale approaches (transcriptomic and meta…

Recovery of strains impaired in their antagonist activity after transposition of the Fot1 element in Fusarium oxysporum

Proteomics as a way to identify extra-radicular fungal proteins from Glomus intraradices - RiT-DNA carrot root mycorrhizas

To identify fungal proteins involved in the arbuscular mycorrhizal symbiosis, root-inducing transferred-DNA transformed roots of carrot (Daucus carota L.) were in vitro inoculated with Glomus intraradices. Proteins extracted from the extra-radical fungus were analysed by two-dimensional gel electrophoresis. A fungal reference map displaying 438 spots was set up. Four proteins, among the 14 selected for tandem mass spectrometry analysis, were identified including a NmrA-like protein, an oxido-reductase, a heat-shock protein and an ATP synthase beta mitochondrial precursor. The possible fungal origin of a MYK15-like protein found in mycorrhizal roots was further discussed. This is the first r…

Vers l'étude quantitative et fonctionnelle des protéomes membranaires des racines mis en jeu au cours de la symbiose mycorhizienne à arbuscules de Medicago truncatula

Phenotype characterization of Fo47 mutants affected in their biocontrol activity

National audience

Label-free 1-DE-LC-MS/MS to identify arbuscular mycorrhiza-related membrane proteins

Deep changes in the shape and number of organelles, together with profound modifications in various membrane compartments, are induced within arbuscular mycorrhizal (AM) symbiosis. In this context, to investigate the membrane-associated proteins that are regulated in the model interaction Medicago truncatula – Rhizophagus irregularis, label-free 1DE-LC-MS/MS approach has been employed as alternative to two-dimensional gel electrophoresis. The existence of a correlation between protein abundance and peak areas or number of MS/MS spectra has widened the choice of label-free quantitative proteomics. The results highlighted microsomal protein candidates that could be involved in the symbiotic e…