New insights into the gut microbiome in loggerhead sea turtles Caretta caretta stranded on the Mediterranean coast

Caretta caretta is the most common sea turtle species in the Mediterranean Sea. The species is threatened by anthropomorphic activity that causes thousands of deaths and hundreds of strandings along the Mediterranean coast. Stranded turtles are often cared for in rehabilitation centres until they recover or die. The objective of this study was to characterize the gut microbiome of nine sea turtles stranded along the Sicilian coast of the Mediterranean Sea using high-throughput sequencing analysis targeting V3–V4 regions of the bacterial 16S rRNA gene. Stool samples were collected from eight specimens hosted in the recovery centre after a few days of hospitalization (under 7) and from one ho…

Some like it cold: the glossiphoniid parasites of the Sicilian endemic pond turtle Emys trinacris (Testudines, Emydidae), an example of ‘parasite inertia’?

The freshwater turtles of the genus Emys and some leech species of the family Glossiphoniidae are the only Palaearctic representatives of primarily Nearctic taxa, which jointly colonized Eurasia and the Maghreb during the Miocene. The strict trophic relationships occurring between the glossiphoniid parasite leech Placobdella costata and its host, the emydid Emys orbicularis, make them a prime example of host–parasite cophylogenetic evolution. In the light of the discovery of the Sicilian cryptic endemic species Emys trinacris, which is the sister species to the widespread Palaearctic E. orbicularis, the possible cophylogenetic divergence of the turtle hosts and their leech parasites was inv…

Presence and biodistribution of perfluorooctanoic acid (PFOA) in Paracentrotus lividus highlight its potential application for environmental biomonitoring

AbstractThe first determination of presence and biodistribution of PFOA in ninety specimens of sea urchin Paracentrotus lividus from two differently contaminated sites along Palermo’s coastline (Sicily) is reported. Analyses were performed on the sea urchins’ coelomic fluids, coelomocytes, gonads or mixed organs, as well as on seawater and Posidonia oceanica leaves samples from the collection sites. PFOA concentration ranged between 1 and 13 ng/L in seawater and between 0 and 794 ng/g in P. oceanica. The analyses carried out on individuals of P. lividus from the least polluted site (A) showed PFOA median values equal to 0 in all the matrices (coelomic fluid, coelomocytes and gonads). Conver…

Helminth Infection of the Loggerhead Sea Turtle Caretta caretta along the Coasts of Sicily and the North West Adriatic Sea

We provide new data on the presence of helminth parasites in 64 individual loggerhead sea turtles Caretta caretta stranded along the coasts of Sicily and the northwest Adriatic Sea between June 2014 and August 2016. The necropsy examination revealed 31 individuals (48.4%) positive for endoparasites, showing a greater prevalence of trematodes than nematodes. In particular, seven species and a single genus of Trematoda (Hapalotrema) and a single species and genus of Nematoda (Kathlania) were identified. Among the Digenea flukes the species with the highest prevalence of infection were Rhytidodes gelatinosus (34.6%) and Hapalotrema sp. (33.3%), while among the Nematoda they were Kathlania sp. …

Genetic diversity data from Salmo trutta collected in Macedonia

Aspetti biologici di Parapenaeus longirostris (Crustacea, Decapoda) nella Sicilia nord occidentale.

First data on the genetic structure of Trachemys scripta populations in Sicily (Testudines: Emydidae)

The pond slider Trachemys scripta is one of the most widespread alien turtle species in the world. Its unregulated trade and the consequent uncontrolled releases into the wild led to negative impacts on the native turtles of the invaded areas. In Italy, alien pond sliders are widely spread, and the occurrence of hatchlings and well-established populations is known in some areas. However, to date in Sicily, only a single female of Trachemys scripta laying eggs was reported. Besides that, nothing is known about the actual reproduction success and establishment of self-sustaining T. scripta populations in Sicily. Therefore, based on 14 previously characterised highly polymorphic microsatellite…

Genetic Variability of Mediterranean Sea red mullet Mullus barbatus (Pisces, Mullidae)

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Wind forcing and fate of <i>Sardinella aurita</i> eggs and larvae in the Sicily Channel (Mediterranean Sea)

Abstract. Multidisciplinary studies are recently seeking to define diagnostic tools for fishery sustainability by coupling ichthyoplanktonic datasets, physical and bio-geochemical oceanographic measurements, and ocean modelling. The main goal of these efforts is the understanding of those processes that control fate and dispersion of fish larvae and eggs and thus tune the inter-annual variability of biomass of fish species. We here analyzed eggs and larvae distribution and biological features of Sardinella aurita in the northeast sector of the Sicily Channel (Mediterranean Sea) collected during the 2010 and 2011 summer cruises. We make use of satellite sea surface temperature, wind, and chl…

DATI PRELIMINARI SULLA STRUTTURA DI POPOLAZIONE DI TURSIOPS TRUNCATUS (CETACEA: ODONTOCETA) NELL'ALTO TIRRENO

Primo contributo alla filogenesi dei diaptomidi paleartico-occidentali (Copepoda, Calanoida)

Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments

Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence and the antimicrobial resistance profile of Arcobacter isolates recovered from different aquatic sources. Besides, we searched for Arcobacter spp. in seaweeds and the corresponding seawater samples. Bacteriological and molecular methods applied to 100 samples led to the isolation of 28 Arcobacter isolates from 27 samples. The highest prevalence was detected in rivers followed by artificial ponds, streams, well waters, and spring waters. Seaweeds contained a higher percentage of Arcobacter than the corresponding seawater samples. The is…

Diversità ed endemismo nel sottogenere Occidodiaptomus, tra conservatorismo morfologico ed evoluzione molecolare (Copepoda: Calanoida: Diaptomidae, genere Hemidiaptomus).

Primi dati di Morfometria Geometrica di Phaleria bimaculata (Coleoptera, Tenebrionidae) nella zona del Mediterraneo centrale.

Morphometric diversity of the mussel Brachidontes pharaonis in different coastal environments

In the debate on how and why biodiversity evolves spatially and temporally and in an attempt to assess the further effects of anthropogenic activities, the ability of marine invertebrate to express a large spectrum of phenotypical plasticity can have a central role. The ability of a single genotype to produce more than one alternative form of morphology and/or physiological state should be larger in species living in highly changing waters like shallow habitats. Invertebrates, there, usually experience ample changes of temperature and food availability on annual, seasonal, diurnal and hourly bases which are the two main drivers leading the life histories of these organisms. In such circumst…

Species identification of the psammophilous tenebrionid beetles Phaleria acuminata Juster, 1852 and Phaleria bimaculata (Linnaeus, 1767) from central Mediterranean beaches: geometric morphometrics and molecular insights from species to population level

Dominating global arid environments, from desert to coastal dunes, most Tenebrionidae are highly specific in their habitat preferences and display limited dispersal potential, thus exhibiting a remarkable degree of regional genetic and morphological differentiation. The tenebrionid genus Phaleria is speciose and widely distributed, with P. acuminata and P. bimaculata having a wide Mediterranean distribution, with numerous morphological differentiations at population level, often described as different taxa of doubtful taxonomical significance. In order to investigate the variability of the central Mediterranean populations of P. bimaculata and P. acuminata and to compare the results obtaine…

On the Occurrence of the Invasive Freshwater Limpet Ferrissia californica (Mollusca: Gastropoda: Planorbidae) in Japan.

Two members of the genus Ferrissia Walker, 1903, namely Ferrissia nipponica (Kuroda, 1949) and F. japonica Habe and Burch, 1965, have been reported to occur in Japan, but due to a lack of molecular data, doubts have been expressed as to their validity. Furthermore, the possible presence of allochthonous Ferrissia taxa has been stated under a variety of names, so that even now there is no consensus on their presence and identity. Recently, freshwater limpets belonging to the planorbid genus Ferrissia were collected in an irrigation trough on the Izu island of Hachijōjima, Tokyo, Japan. Molecular identification of the collected specimens, based on a fragment of the large ribosomal subunit 16S…

Variazione genetica della cernia bruna (Epinephelus marginatus, Pisces) nell’AMP isola di Ustica

The Inland Water Copepod Fauna of a Traditional Rural Landscape in a Mediterranean Island (Crustacea, Copepoda)

Although the Mediterranean area is a well-known hotspot of biological diversity, the crustacean assemblages inhabiting inland waters of the Mediterranean islands are to date unevenly known, and detailed information is missing for most taxa and areas. In the frame of this paper, we provide a checklist and a characterization of the copepod fauna of the lentic water bodies occurring in a traditional rural landscape of Sicily, where the co-existence of agriculture, woodlands, and pastoral activities lead to the presence of a wide range of different aquatic habitats. Overall, 22 copepod species belonging to the orders Calanoida, Cyclopoida, and Harpacticoida have been found in the 92 surveyed si…

Stand out from the Crowd: Small-Scale Genetic Structuring in the Endemic Sicilian Pond Turtle

The geographical pattern of genetic diversity was investigated in the endemic Sicilian pond turtle Emys trinacris across its entire distribution range, using 16 microsatellite loci. Overall, 245 specimens of E. trinacris were studied, showing high polymorphic microsatellite loci, with allele numbers ranging from 7 to 30. STRUCTURE and GENELAND analyses showed a noteworthy, geographically based structuring of the studied populations in five well-characterized clusters, supported by a moderate degree of genetic diversity (FST values between 0.075 and 0.160). Possible explanations for the genetic fragmentation observed are provided, where both natural and human-mediated habitat fragmentation o…

Artificial environmental conditions can affect allozyme genetic structure of the marine gastropod Patella caerulea

Samples of the Mediterranean limpet Patella caerulea collected from 10 sites were examined for genetic population structure. Six of the 20 identified enzymatic loci were polymorphic. The AAT* locus was polymorphic only in two samples from an artificial environment (TI2 and PE). The proportion of polymorphic loci ranged from 0.20 to 0.30, and the observed and expected mean heterozygosity varied between 0.098 and 0.076 and between 0.109 and 0.086, respectively. Mean Fis values were significantly positive in AAT*, ESTD*, PEPC-2*, and PEPD*, showing heterozygosity deficiency. In all, mean Fst value of 0.007 indicated high genetic homogeneity between the samples analyzed, whereas single-locus Fs…

Genetic and morphometric variations of Mediterranean hake,Merluccius merluccius,in the Strait of Sicily (central Mediterranean): Implications for stock assessment of shared resources

Allozyme, morphometric and growth analyses were applied to samples collected in the Strait of Sicily (central Mediterranean Sea) to test the hypothesis of the existence of a unique hake stock (Merluccius merluccius) in the study area. The level of genetic variation was detected from five polymorphic loci (ADH*, PGI-1; PGI-2*, PGM', SOD-1*). The average observed heterozygosity amounted to 0.421, while the average expected was 0.353. Weir & Cockerham statistics showed no heterogeneity, except for the single PGP locus (9 = 0.011; P 0.05). Morphometric analyses revealed some differentiation. Females showed different relationships in six out of eight morphometric indices with total length, while…

Population structure of Brachidontes pharaonis (P. Fisher, 1870) (Bivalvia, Mytilidae) in the Mediterranean Sea, and evolution of a novel mtDNA polymorphism

Abstract Brachidontes pharaonis (Fisher P, 1870) is an Indo-Pacific mussel that has colonized the Mediterranean Sea via the Suez Canal. Mussels may have migrated by natural dispersal of pelagic larvae, or they may have been transported on the hulls of ships, or in ballast water, or by some combination of these. Mitochondrial COI sequences (618 bp) from 101 mussels from six localities in the central and eastern Mediterranean Sea and from one site in the Red Sea were used to describe population structure. Analysis of molecular variance indicated that frequencies differed among populations, and that 92% of the variation resided within populations. The majority of haplotypes were private allele…

Morphological characterization of the blood cells in the endangered Sicilian endemic pond turtle,Emys trinacris(Testudines: Emydidae)

AbstractIn this study, measurements of morphological parameters, sizes and frequencies of peripheral blood cells (erythrocytes, leukocytes, thrombocytes) on blood smear preparation devices stained with May-Grunwald stain were evaluated for both sexes in 20 Emys trinacris (Testudines: Emydidae) specimens. Erythrocytes were higher in male than in female specimens. The leukocyte of E. trinacris contains eosinophil, basophil, monocyte, heterophil and lymphocyte. The eosinophil was higher in males than in females whereas lymphocytes were higher in females than in males. The erythrocyte morphological parameters (EL [erythrocyte length], EW [erythrocyte width], L/W [length/width], ES [erythrocyte …

Are there any distinct genetic sub-populations of sand smelt, Atherina boyeri (Teleostei: Atherinidae) along Italian coasts? Evidence from allozyme analysis

Two hundred and ninety-nine specimens of Atherina boyeri, collected from five Mediterranean sites (three from the open sea (Chioggia, Catania and Gaeta) and two from river mouths (the Birgi and S. Bartolomeo rivers) were analyzed in order to confirm previous genetic studies. Eigtheen loci were scored from 12 enzymatic systems, 3 of which were polymorphic: EST*, PGM* and PGI*. Mean Fst value (0.300, p< 0.05) indicated a high genetic heterogeneity. Pairwise Fst and genetic distance revealed that samples from the river mouths (Birgi and S. Bartolomeo) were grouped separately from the open sea samples (Catania, Chioggia and Gaeta). These results are in agreement with the most recent classificat…

Biodiversity in the era of the market globalization: some cases from the marine realm

The over-exploitation of the most consumed species has determined an increase in demand of frozen or trasformed fishery products importated from different countries. This caused an increase of food fraud represented by the substitution of a species with another with less eco- nomical value but which presents similar morphological characeristics. The use of modern tools as the DNA barcoding is crucial for traceability of such products and provides the consumer the necessary information about the exact identification of the species and their origin. The Italia and European Union food stuff are controlled thanks to laws, while for many non UE products are not expected any control inspection

On the occurrence of the invasive Atlantic blue crab Callinectes sapidus Rathbun 1896 (Decapoda: Brachyura: Portunidae) in Sicilian inland waters

The Atlantic blue crab Callinectes sapidus Rathbun, 1896 is included among the worst invasive alien species in the Mediterranean Sea. Here, we report the finding of the species in two Sicilian rivers, the Irminio and the Imera Meridionale, where it was collected up to 6 km from the river mouths. Although several records of the species are already available from Italy, this is the first evidence of the occurrence of this invasive crab so far from the coastline in the whole country. In the light of the well-known impact of the Atlantic blue crab on the invaded water bodies, the monitoring of the species and appropriate mitigation strategies should be implemented in order to protect the threat…

PRESENZA DI SPECIE CRIPTICHE BRACHIDONTES VARIABILIS-PHARAONIS (BIVALVIA: MYTILIDAE) RIVELATA DALL’ANALISI DEL 28S-rDNA

CHERAX DESTRUCTOR (CLARK, 1836) AND CHERAX QUADRICARINATUS (VON MARTENS, 1868): SAFETY AND NUTRITIONAL QUALITY- Premio "YOUNG INVESTIGATOR AWARD"

Two species of freshwater Parastacidae (Cherax quadricarinatus and Cherax destructor) are used for breeding in several country and to date also in Italy in one aquaculture plant located near Catania (Sicily). Although they are Australian, they seem to have some of the peculiar properties that make it important, strategic and highly appreciated species for aquaculture facilities in Italy. In fact, they mature early, have multiple reproductive cycles in a year, females can lay over a thousand eggs in a single brood and seem to tolerate wide ranges of temperature (Haubrock et al., 2021). Several authors have studied the Cherax genus from different points of view: diseases, moulting phases and …

Different key roles of mesoscale oceanographic structures and ocean bathymetry in shaping larval fish distribution pattern: A case study in Sicilian waters in summer 2009

Fish larvae data collected in year 2009 were used to examine the effects of particular environmental conditions on the structure of larvalassemblages in two oligotrophic Mediterranean areas (the Southern Tyrrhenian Sea and the Strait of Sicily). For this purpose, relationships with environmental variables (temperature, salinity and fluorescence), zooplankton biomass, water circulation and bathymetry are discussed. Hydrodynamic conditions resulted very differently between two study areas. The Southern Tyrrhenian Sea was characterized by moderate shallow circulation compared to the Strait of Sicily. In this framework, distribution pattern of larval density in the Tyrrhenian Sea was mainly dri…

Further investigations on populations of the deep-water blue and red shrimp Aristeus antennatus (Risso, 1816) (Decapoda, Dendrobranchiata), as inferred from Amplified Fragment Length Polymorphism (AFLP) and mtDNA analyses

The aim of this study was to integrate existing mitochondrial DNA data relating to the deep-sea blue and red shrimp Aristeus antennatus (Risso, 1816) with data obtained by Amplified Fragment Length Polymorphism (AFLP). A total of 145 AFLP polymorphic loci were scored in 236 specimens collected from one Atlantic and seven Mediterranean sample sites. AMOVA results revealed that the overall genetic variation among-populations was lower (11.81%) than within-populations (88.19%). The genetic variation between the Atlantic and Mediterranean samples was found to be not significant ( Φ CT = − 0 . 007 ; N.S.), indicating that the transition area between the Atlantic Ocean and the Mediterranean Sea d…

Planktonic stages of small pelagic fishes (Sardinella aurita and Engraulis encrasicolus) in the central Mediterranean Sea: The key role of physical forcings and implications for fisheries management.

Abstract Multidisciplinary studies are recently aiming to define diagnostic tools for fishery sustainability by coupling ichthyoplanktonic datasets, physical and bio-geochemical oceanographic measurements, and ocean modelling. The main goal of these efforts is to understand those processes that control the dispersion and fate of fish larvae and eggs, and thus tuning the inter-annual variability of the biomass of small pelagic fish species. In this paper we analyse the distribution of eggs and larvae as well as the biological features of the two species of pelagic fish, Engraulis encrasicolus and Sardinella aurita in the north-eastern sector of the Sicily Channel (Mediterranean Sea) from ich…

RAD SNP markers as a tool for conservation of dolphinfish Coryphaena hippurus in the Mediterranean Sea: Identification of subtle genetic structure and assessment of populations sex-ratios

Dolphinfish is an important fish species for both commercial and sport fishing, but so far limited information is available on genetic variability and pattern of differentiation of dolphinfish populations in the Mediterranean basin. Recently developed techniques allow genome-wide identification of genetic markers for better understanding of population structure in species with limited genome information. Using restriction-site associated DNA analysis we successfully genotyped 140 individuals of dolphinfish from eight locations in the Mediterranean Sea at 3324 SNP loci. We identified 311 sex-related loci that were used to assess sex-ratio in dolphinfish populations. In addition, we identifie…

On the non-malacostracan crustaceans (Crustacea: Branchiopoda, Copepoda, Ostracoda) from the inland waters of Fthiotida (Greece)

In the frame of the activities of the LIFE11 NAT/GR/1014 ForOpenForests, some water bodies occurring in the "Ethnikos Drymos Oitis" (GR2440004) and "Oros Kallidromo" (GR2440006) (Sterea Ellada) were investigated with the aim of providing a first census of the composition and diversity of their crustacean fauna. Overall, the sampling of 15 water bodies (7 of them listed as "Mediterranean temporary ponds" sensu EU "Habitats Directive") led to the finding of 13 branchiopod, 11 copepod, and 7 ostracod taxa, including 4 species new for mainland Greece, i.e. the copepods Arctodiaptomus alpinus (Imhoff, 1885) and Diaptomus cf. serbicus, and the branchiopods Leptestheria dahalacensis (Rüppel, 1837)…

No evidence of DUI in the Mediterranean alien species Brachidontes pharaonis (P. Fisher, 1870) despite mitochondrial heteroplasmy

AbstractTwo genetically different mitochondrial haplogroups of Brachidontes pharaonis (p-distance 6.8%) have been identified in the Mediterranean Sea. This hinted at a possible presence of doubly uniparental inheritance in this species. To ascertain this possibility, we sequenced two complete mitogenomes of Brachidontes pharaonis mussels and performed a qPCR analysis to measure the relative mitogenome copy numbers of both mtDNAs. Despite the presence of two very similar regions composed entirely of repetitive sequences in the two haplogroups, no recombination between mitogenomes was detected. In heteroplasmic individuals, both mitogenomes were present in the generative tissues of both sexes…

Distribuzione di Bufo balearicus in Sicilia e rinvenimento di una zona ibrida tra le specie B. balearicus e B. siculus.

Biochemical genetic differentiation between Pomatoschistus marmoratus and P. tortonesei

Several diagnostic genetic markers were identified in Pomatoschistus marmoratus and P. tortonesei using polyacrylamide gel electrophoresis (PAGE) of allozymes. Twenty-one loci were resolved, including the electrophoretic pattern of muscle proteins. The MDH*, PGM1,2*, EST-1,2*, FUM* and PGI-2* loci exhibited different alleles which were fixed for the two species being analysed. Genetic distance, as calculated by Nei's index, showed a value of 0.413. Environmental hypersalinity, could have influenced the geographical distribution of P. tortonesei.

Due casi-studio di “stock identification” con microsatelliti nel Mar Mediterraneo: la triglia di fango Mullus barbatus e il nasello Merluccius merluccius.

The DNA barcoding of Mediterranean combtooth blennies suggests the paraphyly of some taxa (Perciformes, Blenniidae)

A dataset including novel and publicly available mtDNA COI sequences of 14 Mediterranean combtooth blenny species belonging to nine genera was assembled in order to provide a reference dataset for DNA barcoding studies. Some inconsistencies in the current taxonomy of some genera were observed. In particular, the monophyly of the genera Parablennius and Salaria is not supported by the present dataset and the absence of reciprocal monophyly between the morphospecies Salaria basilisca and S. pavo questions their status and stresses the need of a revision of the genus Salaria.

Isolamento per distanza in popolazioni mediterranee di Parapenaeus longirostris (Crustacea: Decapoda).

Biochemical taxonomic differentiation between Mullus barbatus and mullus surmuletus (Pisces, mullidae)

Abstract 1. 1. The electrophoretic data from seven enzymatic systems, codifying for 20 loci, and the patterns of general proteins from some tissues of Mullus barbatus and Mullus surmuletus were examined. 2. 2. The value of the genetic distance index as per Nei is 0.068, this criterium showing a high similarity between the two species. 3. 3. Species-specific patterns were found for the SOD enzyme and general proteins of the muscle.

Un primo contributo alla filogeografia del ghiro (Glis glis) in italia.

The valid genus name of the European freshwater blennies, Ichthyocoris or Salariopsis (Teleostei: Blenniidae)?

Vecchioni, Luca, Arculeo, Marco, Hundt, Peter J., Marrone, Federico (2022): The valid genus name of the European freshwater blennies, Ichthyocoris or Salariopsis (Teleostei: Blenniidae)? Zootaxa 5162 (1): 99-100, DOI: https://doi.org/10.11646/zootaxa.5162.1.8

Range expansion and climate warming: state-of-art and perspectives of the case-study Brachidontes pharaonis (Mollusca: Mytilidae).

First record of Sphoeroides pachygaster (Tetraodontidae) in the south Tyrrhenian (N/W Sicily)

Analisi dell’rDNA 12S nelle relazioni sistematiche della Lucertola campestre (Podarcis sicula) in Italia.

Variabilità genetica in Aristeus antennatus (Crustacea, Decapoda) attraverso analisi AFLP (Amplified Fragment Length Polymorphism).

Are there autochthonous Ferrissia (Mollusca: Planorbidae) in the Palaearctic? Molecular evidence of a widespread North American invasion of the Old World

In the last few decades, findings of freshwater limpets belonging to the genus Ferrissia have been frequent and widely distributed throughout the Palaearctic and beyond. The widespread presence of a Nearctic alien taxon was proved, but no consensus was achieved about the possible existence of autochthonous Ferrissia taxa in the area, an occurrence which would be supported by the presence of gastropod fossils attributed to the genus throughout Eurasia and North Africa. In order to test the hypothesis of a possible persistence of autochthonous Ferrissia taxa in the Palaearctic to the present day, all the published data on the genetic diversity of Ferrissia populations occurring in the area we…

Growth and reproduction of the deep-water rose shrimp, Parapenaeus longirostris (Lucas, 1846) (Decapoda, Penaeidae), in the southern Tyrrhenian sea.

From June 2006 to May 2007, monthly samples of Parapenaeus longirostris (Lucas, 1846) were collected on land from the landings of two different, although contiguous, fishing grounds exploited by the bottom trawl fisheries of the two fishing harbours of Terrasini and Porticello, located on the north-western coast of Sicily. Carapace length (CL) of the female and male deep-water rose shrimp in Terrasini ranged from 9 to 32 mm and 13 to 26 mm, respectively, whereas in Porticello the length ranged from 8 to 31 mm and 13 to 26 mm, respectively. The Von Bertalanffy Growth Function parameters for Terrasini females and males were CLoo = 38.5 mm, K = 0.65 year(-1) and CL infinity = 32.5 mm, K = 0.85…

Mitochondrial simple sequenze repeats and 12s – rRNA gene reveal two distinct lineages of Crocidura russula (Mammalia, Sorcidae)

A short segment (135 bp) of the control region and a partial sequence (394 bp) of the 12S-rRNA gene in the mitochondrial DNA of Crocidura russula were analyzed in order to test a previous hypothesis regarding the presence of a gene flow disruption in northern Africa. This breakpoint would have separated northeast-African C. russula populations from the European (plus the northwest-African) populations. The analysis was carried out on specimens from Tunisia (C. r. cf agilis), Sardinia (C. r. ichnusae), and Pantelleria (C. r. cossyrensis), and on C. r. russula from Spain and Belgium. Two C. russula lineages were identified; they both shared R2 tandem repeated motifs of the same length (12 bp)…

Struttura di popolazione della triglia di fango, Mullus barbatus (Pisces: Mullidae), nel Mar Mediterraneo rivelata dall’analisi dei microsatelliti

DNA barcoding for species assignment: the case of Mediterranean marine fishes

Background: DNA barcoding enhances the prospects for species-level identifications globally using a standardized and authenticated DNA-based approach. Reference libraries comprising validated DNA barcodes (COI) constitute robust datasets for testing query sequences, providing considerable utility to identify marine fish and other organisms. Here we test the feasibility of using DNA barcoding to assign species to tissue samples from fish collected in the central Mediterranean Sea, a major contributor to the European marine ichthyofaunal diversity. Methodology/Principal Findings: A dataset of 1278 DNA barcodes, representing 218 marine fish species, was used to test the utility of DNA barcodes…

A hybrid zone of two deeply diverged green toad taxa in north-eastern Sicily.

Impact of Heavy Metals in Eggs and Tissues of C. caretta along the Sicilian Coast (Mediterranean Sea)

In this study we compared the heavy metal concentration found in different tissues and eggs of the loggerhead sea turtle and evaluated the potential ecotoxicological risk for this important species. Eighteen heavy metal elements were determined in different tissues (liver, gonads, fat, kidney, heart, brain, and spleen) of nine individuals of Caretta caretta found stranded along the coasts of Messina (Sicily, Italy) and in the shell and yolk of six eggs from the island of Linosa (Sicily, Italy). For the analysis of the heavy metals, we used the analytical procedures in accordance with the EPA 200.8 method supplemented by EPA 6020b with three replicates for each measurement. The elements anal…

Cryptic species within the freshwater copepod genus Hemidiaptomus (calanoida, diaptomidae).

The copepod genus Hemidiaptomus is composed by about 17 large-bodied species inhabiting the freshwater temporary water bodies of the whole Palaearctic region. It currently comprises three subgenera, which are mostly parapatric and present only limited co-occurrence areas. There is no consensus on the taxonomical arrangement of the entire group, and to date no molecular studies have been carried out to test it. Accordingly, a thorough revision of the entire genus using a combined morphological and molecular approach is strongly advisable. We have thus decided to investigate the pattern of morphological and molecular diversity of three species of the genus Hemidiaptomus belonging to the three…

Growth parameters and population structure of Aristeus antennatus (Decapoda, Penaeidae) in the south Tyrrhenian Sea (southern coast of Italy).

Abstract The blue and red shrimp Aristeus antennatus (Risso, 1816) is one of the most important fishery resources in the Mediterranean Sea. Monthly samplings of blue and red shrimp from June 2006 to May 2007 were landed by the trawl fleet in two northwest Sicilian fishing harbours (San Vito Lo Capo and Terrasini). The carapace length (CL) frequency distribution of females ranged between 15.00 and 59.00 mm, whereas male CLs ranged between 17.00 and 34.00 mm. The estimated parameters of the Von Bertalanffy growth function (VBGF) for San Vito lo Capo females and males were: CL∞ = 65 mm, K = 0.58 y–1 and CL∞ = 41 mm, K = 0.71 y–1, respectively; while for Terrasini females and males these were:…

Food partitioning between Serranus scriba and Scorpaena porcus (Perciformes) on the infralittoral ground of the South Tyrrhenian Sea.

Food preferences of Scorpaena porcus and Serranus scriba were investigated from the stomach contents of fish caught with trammel nets in the Gulf of Palermo (Tyrrhenian Sea). Decapoda Natantia and Decapoda Brachyura were the main preferred prey in the diet of S. porcus whereas Decapoda Natantia, Decapoda Galatheidae and Pisces were the preferred prey for S. scriba. These results and the values of the index of overlapping alimentary requirements suggested competition for food resources between the two species. A more precise investigation, at species level, of the food remains of the most abundant taxon (Crustacea Decapoda) made it possible to demonstrate that the trophic niche overlap betwe…

Green toads from most of Sicily are an old sister taxon of african green toads and represent a new european vertebrate species (Bufo n. sp.).

Can DNA methylation contribute to explain morphological variability? The case of loggerhead turtle Caretta caretta

The loggerhead turtle Caretta caretta is a common and threatened sea turtle in the Mediterranean Sea. It is listed in the Red List of Threatened Species of the International Union for Conservation of Nature (IUCN 2012) and, although it is widely studied, little is currently known about the relationships between its phenotype and genetic variability. In the last few years some authors observed the presence of individuals, collected in different parts of the Mediterranean Sea, with a variable number of carapacial and plastron scutes. This variability, in some cases, makes difficult the sound identification of the species (Margaritoulis and Chiras, 2011; Turkozan et al. 2001; Oliver 2014). To …

Congruence in genetic markers used to describe Mediterranean and Atlantic populations of European hake (Merluccius merluccius L. 1758)

Summary Eight samples of the hake, Merluccius merluccius L., from the Mediterranean basin (370 fishes total) and one from the Atlantic ocean (50 fishes) were analysed in order to assess genetic variability and describe genetic population structure. Five polymorphic protein coding loci were scored (ADH*, PGI-1*, PGI-2*, PGM* and SOD-1*) in eight samples, together with a haplotype variation of four samples, obtained from polymerase chain reaction/restriction fragment length polymorphism (PCR–RFLP) analysis on the mitochondrial DNA control region. The average value for observed heterozygosity was typically higher than expected (showing an excess of heterozygotes among the samples) whereas the …

Analyses of the spatial genetic structure of different Phaleria bimaculata (Coleoptera: Tenebrionidae) populations from central Mediterranean sandy beaches

The late Pleistocene origin of the Italian and Maltese populations of Potamon fluviatile (Malacostraca: Decapoda): insights from an expanded sampling of molecular data

Evidence available for most inland water and terrestrial organisms highlights the significant role played by southern Italy, Sicily and the Maltese islands as refuges during Pleistocene climatic fluctuations. However, to date, the hypothesis that these areas may have acted as Pleistocene refugia for the freshwater crab Potamon fluviatile has not been explicitly tested, and a recent origin of local P. fluviatile populations was proposed on the basis of a small set of analysed molecular data. We have thus expanded the currently available data set on the population genetic structure of P. fluviatile through dedicated samplings in Sicily (Italy, 18 specimens), the Maltese Islands (Malta, 15 spe…

Cryptic invasion in Southern Europe: The case of Ferrissia fragilis (Pulmonata: Ancylidae) Mediterranean populations

Four populations of the North American ancylid gastropod Ferrissia fragilis have been found in Southern Italy, thus expanding the known Eurasian distribution range of this invasive gastropod to the Mediterranean area. Both mitochondrial markers used for the molecular identification of the sampled specimens (16S and COI) showed a modest to absent haplotypic diversity in the studied Ferrissia populations from Europe and Asia, and their perfect identity with some of the haplotypes observed in North American F. fragilis populations. In the light of the scarce molecular diversity observed in the whole Palaearctic area and of the growing evidences for the ongoing spreading of the species in Europ…

Isolation By Distance (IBD) signals in the deep-water rose shrimp Parapenaeus longirostris (Lucas, 1846) (Decapoda, Panaeidae) in the Mediterranean Sea

Abstract The identification of boundaries of genetic demes is one of the major goals for fishery management, and few Mediterranean commercial species have not been studied from a genetic point of view yet. The deep-water rose shrimp Parapenaeus longirostris (Lucas, 1846) is one of the most important components of commercial landings in Mediterranean, its fishery aspects have received much attention, regrettably without any concern for the genetic architecture of its populations. The population structure in the central and eastern Mediterranean Sea (captures from six Italian and two Greek landings) has been analysed on the basis of surveys carried out with mitochondrial and AFLP markers. Dat…

New records of leatherback sea turtle, Dermochelys coriacea (Vandelli, 1761) (Testudines: Dermochelyidae) in the Strait of Sicily

We report three new records of leatherback sea turtle, Dermochelys coriacea (Vandelli, 1761) recovered in February 2011 and in spring-summer 2016 in the Strait of Sicily. The individuals were handed over dead to the IZS of Palermo for necropsy. The first specimen measured 139 and 91 cm of CCL and CCW respectively and was found beached and alive but died a few hours later for septicemia. The second measured 110 cm of CCL and 83 cm of CCW and showed a deep wound on its head probably due to contact with a boat propeller. The third individual measured 134 cm of CCL and 96 cm of CCW and had plastic bags, a piece of fishing net and debris of various nature in the stomach. For the second individua…

New distributional data on &lt;em&gt;Haemogregarina stepanowi&lt;/em&gt; (Apicomplexa) and &lt;em&gt;Placobdella costata&lt;/em&gt; (Hirudinea) parasitising the Sicilian pond turtle &lt;em&gt;Emys trinacris&lt;/em&gt; (Testudines)

The host-parasite system “Emys trinacris – Placobdella costata – Haemogregarina stepanowi” is known for Sicily, but scarce information is available to date about the distribution of the two parasites on the island. Therefore, an extensive sampling effort through visual census and collection and analysis of blood smears of the endemic Sicilian pond turtle E. trinacris was carried out in 46 water bodies scattered throughout mainland Sicily. Our findings revealed that the distribution of both parasites is limited to the Nebrodi area, where the infection of H. stepanowi has shown a high incidence on the local turtle populations. Our data suggest no correlation between the current distribution o…

Two seas, two lineages: How genetic diversity is structured in Atlantic and Mediterranean greater amberjack Seriola dumerili Risso, 1810 (Perciformes, Carangidae)

The vastness of the ocean and our limited ability to observe and monitor the organisms therein make molecular markers particularly useful tools in the investigation of the ecology and evolutionary biology of fish. Nowadays, due to increased demand of fish foods, genetic researches are focused on new candidate species for aquaculture, such as Seriola species. Here, the analyses of eight polymorphic nuclear microsatellites loci and three mitochondrial (mt) DNA genes (16S-rRNA, Cyt-b, and D-Loop) in the greater amberjack, Seriola dumerili, detected breakpoint between Atlantic and Mediterranean populations, presumably arisen by the oceanographic features of the Almeria-Oran Front opposed to the…

Differenziazione genetica tra popolazioni mediterranee del Crostaceo Decapode Parapenaeus longirostris.

A molecular approach to assess the genealogical relationship among some mesopelagic fishes

Overlooked cryptic endemism in copepods: Systematics and natural history of the calanoid subgenus Occidodiaptomus Borutzky 1991 (Copepoda, Calanoida, Diaptomidae)

Abstract Our comprehension of the phylogeny and diversity of most inland–water crustaceans is currently hampered by their pronounced morphological bradytely, which contributed to the affirmation of the “Cosmopolitanism Paradigm” of freshwater taxa. However, growing evidence of the existence of cryptic diversity and molecular regionalism is available for calanoid copepods, thus stressing the need for careful morphological and molecular studies in order to soundly investigate the systematics, diversity and distribution patterns of the group. Diaptomid copepods were here chosen as model taxa, and the morphological and molecular diversity of the species belonging to the west-Mediterranean diapt…

A survey of ovarian maturation in a population of Aristeus antennatus [Crustacea: Decapoda)

Andamento delle catture di Aristeus antennatus (Risso, 1816) nella Sicilia settentrionale attraverso dati di sbarco.

The good, the bad and the ugly: Emys trinacris, Placobdella costata and Haemogregarina stepanowi in Sicily (Testudines, Annelida and Apicomplexa)

Endemic Sicilian pond turtles Emys trinacris Fritz, Fattizzo, Guicking, Tripepi, Pennisi, Lenk, Joger et Wink were examined for the presence of haemogregarine parasites. The presence of haemogregarines, occurring mainly in the microgametocyte stage (13.2 ± 0.12 μm in length and 6.4 ± 0.52 μm in width), was observed in approximately 9% of the sampled E. trinacris. Based on the observed morphology and on the sequencing of nuclear 18S rDNA, we identified the parasite as Haemogregarina stepanowi Danilewsky, 1885. Morphometric study of uninfected and infected red blood cells has shown that H. stepanowi induces different changes in erythrocyte shape depending on the infective stage. The different…

Studio della variabilità genetica di Melicertus kerathurus (Crustacea, Decapoda), catturato in mar mediterraneo e nel nord-est atlantico attraverso il mtDNA

Identificazione di stock di Mullus barabtus (Teleostei: Perciformes) lungo le coste siciliane attraverso l'uso di microsatelliti.

A new record and molecular characterization of the Green Turtle Chelonia mydas (Testudines, Cheloniidae) in Sicilian coastal waters

A juvenile specimen of the Green Turtle, Chelonia mydas, was recovered in Sicilian coastal waters close to Avola (Syracuse province, south-eastern Sicily, Italy). Before being released, the specimen was measured and inspected for the presence of ingested hooks or other possible harms to its health. A fragment of the mtDNA marker COI was amplified and sequenced in order to provide the first molecular data for the species from the central Mediterranean area. The possible influence of climate change on the occurrence of this thermophilic species in the central Mediterranean area is briefly discussed.

Revision of Recent and fossil Mixtacandona Klie 1938 (Ostracoda, Candonidae) from Italy, with description of a new species

Although studies on ostracods dwelling in inland subterranean habitats of Italy have increased in the last decades, highlighting a considerable taxonomic diversity, available information is still far from sufficient to understand phylogenetic and biogeographic relationships. Mixtacandona Klie 1938 is one of the most common and diverse genera of ostracods in subterranean waters. Of the 20 living recognized species in the genus, all stygobiontic and restricted to the Palearctic Region, four are known for the Italian peninsula and Sardinia, one of which exclusively as fossil. Several other Recent and fossil taxa attributable to Mixtacandona, but identified at supraspecific level, have been rep…

Genetic variation of dusky grouper (Epinephelus marginatus, Lowe 1834).

Summer aggregation of common eagle ray, Myliobatis aquila (Chondrichthyes: Myliobatidae), in the Marine Protected Area of the Egadi Islands (southwestern Tyrrhenian Sea)

Author(s): Grancagnolo, Desiree; Arculeo, Marco | Abstract: We report for the first time in Italian waters a periodic and frequent presence of a summer aggregation of common eagle ray Myliobatis aquila in the island of Marettimo (southwestern Tyrrhenian Sea), which is part of the Marine Protected Area of the Egadi Islands. These aggregation could be related to different phenomena such as reproduction, feeding or simply as a transit zone.

Investigations on gonadal maturation in Pagellus acarne (Pisces, Sparidae) in the strait of Messina (Sicily)

I DECAPODI BERSAGLIO DELLA FLOTTA ARTIGIANALE TRA CAPO GRANITOLA E CAPO SAN MARCO (COSTA SUD OCCIDENTALE SICILIANA)

Isolation and use of microsatellite loci in Melicertus kerathurus (Crustacea, Penaeidae).

Melicertus kerathurus represents an economically important resource for fisheries and aquaculture. Seven microsatellite loci from 373 specimens of M. kerathurus collected in different parts of the Mediterranean and NE Atlantic were identified. Four of these microsatellites showed a moderate level of polymorphism, with 2 to 14 alleles per locus, whereas three had a monomorphic profile. Observed and expected heterozygosity ranged between 0.28 to0.64 and 0.28 to 0.65, respectively. Three microsatellite loci deviated from Hardy-Weinberg equilibrium in some populations, with a deficit of heterozygosity. Mean Fst values showed significant differentiation among sample sites analysed and indicate t…

First record of the alien leech Myzobdella lugubris Leidy, 1851 (Hirudinea, Piscicolidae) in the Palearctic

espanolEn este estudio reportamos la aparicion de la sanguijuela Neartica Myzobdella lugubris en un cuerpo de agua salobre de la reserva natural Riserva Naturale dello Stato “Le Cesine” (Apulia, Italy). Dos especimenes de la especie fueren recolectados en el cuello y las extremidades anteriores de una tortuga de estanque europea Emys orbicularis hellenica en Octubre del 2016. La identificacion de los dos especimenes fue basada en datos morfologicos y de secuenciacion molecular. En su distribucion natural, M. lugubris es conocida por ser huesped y vector de bacterias y virus, de los cuales algunos son patogenos de gravedad en peces. En vista de los posibles efectos nocivos, que ejercen sobre…

Diversity and Distribution of the Inland Water Decapods of Sicily (Crustacea, Malacostraca)

The current knowledge of Sicilian inland water decapod malacostracans is scarce and an updated synopsis on species distribution is lacking. Therefore, we reviewed the checklist and recent distribution of Sicilian inland water decapods based on published and unpublished records and novel observations with the aim of providing an exhaustive repository, also to be used as a sound baseline for future surveys. Overall, five native decapod species occur in the study area, i.e., the atyid shrimp Atyaephyra desmarestii, the palaemonid shrimps Palaemon adspersus, P. antennarius, and P. elegans, and the freshwater crab Potamon fluviatile, and their current local distributions are described. In additi…

La fauna a diaptomidi di Israele

A Comparative Analysis of Aquatic and Polyethylene-Associated Antibiotic-Resistant Microbiota in the Mediterranean Sea

Simple Summary In recent years, a growing interest has been devoted to the bacterial characterization of marine plastic debris. So far, a few publications have explored the composition of microbial communities on polyethylene (PE) waste items and the occurrence of antibiotic-resistant bacteria (ARB). The occurrence of ARB in natural matrices can contribute to the spread of antibiotic resistance genes (ARGs) among environmental bacteria. In this study, we compared the microbial composition and the presence of ARGs in water and PE fragments collected from a stream and the seawater in a coastal area of Northwestern Sicily. Our findings showed more ARGs on PE fragments than the corresponding wa…

Assessment of inbreeding in hatchery samples of the red seabream Pagrus major (Perciformes: Sparidae).

The marine crustacea decapoda of Sicily: a cecklist with remarks on their distribution.

A checklist of the Crustacea Decapocla of Sicily, based on previously published records and on original data, is presented. The Sicilian waters were divided into three sectors to account for the different habitats present along the northern and western (sector A), eastern (B) and southern (C) coasts, for the sake of a better interpretation of the species distribution. A total of 186 species, vas recorded between 0 and 800 in depth, 31 of which are reported here for the first time. Sector A hosted 153 species, B 105 species. and C 117 species. The dissimilarities among the habitats present in the shallow waters of the three sectors accounted for the low co-occurrence of species: 30 species w…

Incidental catch of loggerhead sea turtles (Caretta caretta) along the Sicilian coasts by longline fishery

It has been estimated that 44,000 Caretta caretta turtles die every year due to anthropomorphic activity in the Mediterranean Sea, and that longline fishing is one of the most significant causes of mortality. A total of 482 specimens of C. caretta were rescued from different parts of the Sicilian coast (Mediterranean Sea) from 2014 to 2016. The most numerous stranding was recorded during the spring and summer seasons, mainly along the north and eastern coasts of Sicily. The curved carapace length for all the specimens ranged from between 19 and 95 cm and most of these were young or sub adults. The highest number of strandings was recorded in 2014 and 2015, with 206 and 169 individuals, resp…

Post-Messinian evolutionary relationships across the Sicilian channel: Mitochondrial and nuclear markers link a new green toad from Sicily to African relatives

19 páginas, 6 figuras y 3 tables

Aspetti riproduttivi di Hoplostethus mediterraneus nel mediterraneo centrale

Improving the conservation of Mediterranean Chondrichthyans : the ELASMOMED DNA barcode reference library

Cartilaginous fish are particularly vulnerable to anthropogenic stressors and environmental change because of their K-selected reproductive strategy. Accurate data from scientific surveys and landings are essential to assess conservation status and to develop robust protection and management plans. Currently available data are often incomplete or incorrect as a result of inaccurate species identifications, due to a high level of morphological stasis, especially among closely related taxa. Moreover, several diagnostic characters clearly visible in adult specimens are less evident in juveniles. Here we present results generated by the ELASMOMED Consortium, a regional network aiming to sample …

New contribution to the systematic status of various Mediterranean scorpionfish, as inferred from a mitochondrial DNA sequence.

This study investigated the molecular phylogeny of 6 Mediterranean species of scorpionfish, belonging to the Scorpaenidae and Sebastidae family. Neighbor-Joining and Maximum Parsimony phylogenetic analyse, based on 424 base pairs of partial mitochondrial DNA sequences of the 12S-rRNA gene, revealed 2 main clades. One clade is represented by the Scorpaena genera (with the species S. notata, S. porcus, and S. scrofa) and another clade consists of the genera Helicolenus, Pterois, and Scorpaenodes. The molecular phylogeny showed that the Scorpaenodes genus (sub-family Scorpaeninae) is found within the clade of the species belonging to the other two sub-families (Pteroninae and Sebastinae). This…

Dati sulla caratterizzazione di loci AFLP utili per lo studio della variazione genetica di Parapenaeus longirostris (Crustacea, Decapoda).

Differences in gene flow in a twofold secondary contact zone of pond turtles in southern Italy (Testudines: Emydidae:Emys orbicularis galloitalica,E. o. hellenica,E. trinacris)

Using virtually range-wide sampling for three pond turtle taxa (Emys orbicularis galloitalica, E. o. hellenica, E. trinacris), we analyse gene flow across their southern Italian contact zone. Based on population genetic analyses of 15 highly polymorphic microsatellite loci and a mitochondrial marker, we show that the general genetic pattern matches well with the current taxon delimitation. Yet, single individuals with conflicting genetic identity suggest translocation of turtles by humans. In addition, we identify in south-western France and the vicinity of Rome populations being heavily impacted by introduced turtles. Cline analyses reveal that the major genetic break between E. o. galloit…

Molecular data attest to the occurrence of autochthonous Daphnia pulex (Crustacea, Branchiopoda) populations in Sicily, Italy

Biological invasions are known to be among the most important threats to the long-term conservation of native biota, and their effects might be even more difficult to contrast when they are cryptic, i.e., when the non-native invaders cannot be easily recognised based on morphology, and can thus be confused with native taxa. Such cryptic invasions are known to widely occur in the cladoceran genus Daphnia O.F. Müller, 1785, so that the actual distribution and status of most species and lineages need to be checked with a genetic approach. In the frame of this work, we investigated if the Sicilian populations of D. (Daphnia) pulex Leydig, 1860 belonged to the allochthonous North American lineag…

On the occurrence of Eudiaptomus gracilis (G.O. Sars, 1863) (Copepoda: Diaptomidae) in Madeira (Portugal)

Calanoid copepods belonging to the diaptomid genus Eudiaptomus Kiefer, 1932 were collected in an artificial water body on the island of Madeira, Portugal. The morphological and molecular identification of the collected specimens unequivocally proved that they belong to the allochthonous species Eudiaptomus gracilis (G. O. Sars, 1863), possibly unwarily introduced along with fish stockings or ornamental aquatic plants. In the sampled site, the species co-occurred with the non-native cyclopoid copepod Acanthocyclops americanus (Marsh, 1893) and some anomopod species. This is the first record of the species for Portugal and the whole Macaronesia, whereas the possible presence of E. gracilis in…

Absence of spatial genetic structure in common dentex (Dentex dentex Linnaeus, 1758) in the Mediterranean Sea as evidenced by nuclear and mitochondrial molecular markers.

International audience; The common dentex, Dentex dentex, is a fish species which inhabits marine environments in the Mediterranean and Northeast Atlantic regions. This is an important species from an ecological, economic and conservation perspective, however critical information on its population genetic structure is lacking. Most samples were obtained from the Mediterranean Sea (17 sites) with an emphasis around Corsica (5 sites), plus one Atlantic Ocean site. This provided an opportunity to examine genetic structuring at local and broader scales to provide science based data for the management of fishing stocks in the region. Two mitochondrial regions were examined (D-loop and COI) along…

Genetic and morphometric variation of Mediterranean hake, Merluccius merluccius, in the Strait of Sicily (Central Mediterranean)

Allozyme, morphometric and growth analyses were applied to samples collected in the Strait of Sicily (central Mediterranean Sea) to test the hypothesis of the existence of a unique hake stock (Merluccius merluccius) in the study area. The level of genetic variation was detected from five polymorphic loci (ADH*, PGI-1*, PGI-2*, PGM*, SOD-1*). The average observed heterozygosity amounted to 0.421, while the average expected was 0.353. Weir & Cockerham statistics showed no heterogeneity, except for the single PGI? locus (theta = 0.011; P 0.05). Morphometric analyses revealed some differentiation. Females showed different relationships in six out of eight morphometric indices with total length,…

The marine Crustacea Decapoda of Sicily (central Mediterranean Sea): A checklist with remarks on their distribution

A checklist of the Crustacea Decapoda of Sicily, based on pre­ viously published records and on original data, is presented. The Sicilian waters were divided into three sectors to account for the different habitats present along the northern and western (sector A), eastern (B) and southern (C) coasts, for the sake of a better interpretation of the species distribution. A total of 186 species was recorded between 0 and 800 m depth, 31 of which are re­ ported here for the first time. Sector A hosted 153 species, B 105 species, and C 117 species. The dissimilarities among the habitats present in the shallow waters of the three sectors accounted for the low co-occurrence of species: 30 species …

A molecular approach to assess the population structure of Pomatoschistus tortonesei in the Mediterranean Sea.

DNA taxonomy of the potamid freshwater crabs from Northern Africa (Decapoda, Potamidae)

The Mediterranean area, from southern Balkans to western Maghreb, is inhabited by the Potamon subgenus Euthelphusa, with three currently recognised species. The Maghrebian species P. (E.) algeriense is isolated from other Potamon species by the Mediterranean Sea and the Sahara Desert, and nearly no molecular data are currently available for this taxon. Present study investigated the mtDNA and nuDNA diversity in Potamon algeriense s.l. with the aims of exploring its molecular diversity pattern throughout its known distribution range, and testing the possible presence of cryptic taxa currently lumped under this binomen. The phylogenetic and DNA taxonomy analyses showed the presence of two wel…

Allozymic variation in Mediterranean hake, Merluccius merluccius (Gadidae)

Abstract Four hundred and twenty individual hake from 10 sample sites in the Mediterranean Sea were analysed in order to study genetic variability and identify genetic stock structure. Twenty loci were identified, four of which were polymorphic at the 95% level: ADH*, PGI‐1*, PGI‐2* and SOD‐1*. Average observed and expected heterozygosity were 0.084 and 0.090, respectively. PG1–1* deviated from Hardy‐Weinberg expectations due to an excess of heterozygotes and F‐statistic analysis showed also a significant excess of heterozygosity at SOD‐1*. FST was not significant for each locus except for PGI‐2*, where a single sample from the Channel of Sicily (C5) showed a different pattern in allelic fr…

Studio della struttura genetica di Melicertus kerathurus attraverso l’uso di marcatori molecolari.

The Taxonomy Lab: a sentinel for Mediterranean bioinvasions

Morphological and molecular tools in identifying the Mediterranean limpets Patella caerulea, Patella aspera and Patella rustica

Allozyme electrophoresis, a partial nucleotide sequence of a mitochondrial gene for cytochrome oxidase I (COI) and discriminant analysis of shell morphometric characters were used to study the relationships among the Sicilian marine gastropods of the Patella genus. Allozyme and mtDNA markers unequivocally distinguished the species and were very useful markers in correctly classifying the different species when morphological characters overlapped each other. Several allozyme loci and many nucleotide positions were diagnostic of species. In contrast, the discriminant analysis of simple morphometric shell characters failed to adequately discriminate the species, suggesting that environmental f…

Due casi studio di stock identification con microsatelliti nel mar Mediterraneo: la triglia di fango (Mullus barbatus) e il nasello (Merluccius merluccius)

The Mediterranean Sea hosts endemic haplotypes and a distinct population of the dolphinfish Coryphaena hippurus Linnaeus, 1758 (Perciformes, Coryphaenidae)

Abstract The dolphinfish, Coryphaena hippurus Linnaeus, 1758, is an important target species for Mediterranean artisanal, recreational and commercial fisheries but to date only scarce genetic data are available for its Mediterranean population(s). The genetic variation of Mediterranean dolphinfishes was thus investigated through the sequencing of fragments of the cytochrome c oxidase subunit 1 (COI) gene and the NADH dehydrogenase subunit 1 (ND1) mitochondrial DNA markers with the explicit aims of (i) testing for significant genetic differentiation of the Mediterranean vs . non-Mediterranean populations of the species, and (ii) investigating the possible presence of molecular structuring wi…

New record in the Mediterranean Sea of unusual skin colouration in the black-bellied anglerfishLophius budegassaSpinola, 1807

Molecular evidence for the presence of cryptic evolutionary lineages in the freshwater copepod genus Hemidiaptomus G.O. Sars, 1903 (Calanoida, Diaptomidae)

The pattern of morphological and mtDNA cytochrome b diversity of three calanoid copepod species belonging to the diaptomid genus Hemidiaptomus has been investigated with the aim of checking the reliability of the morphological characters currently used for species identification, and the possible presence of cryptic taxa. A sharply different molecular structuring has been observed in the studied species: while Hemidiaptomus amblyodon exhibits a remarkable constancy throughout the European range of its distribution area (maximum inter-populations cytochrome b divergence of 3%), observed distances between presumed conspecific lineages of Hemidiaptomus gurneyi (maximum divergence of 21.5%) and…

Première occurrence de la sous-espèce de rouget Mullus barbatus ponticus au Sud de la Méditerranée (golfe de Gabès, Tunisie) : arguments génétiques et morphométriques

New molecular data attest to the absence of cospeciation patterns between Placobdella costata (Fr. Müller, 1846) (Hirudinea) and freshwater turtles (Emys spp.) in Italy

The only Palearctic representative of the leech genus Placobdella Blanchard, 1893 is P. costata, an ectoparasite of freshwater turtles. To date, no conclusive evidence about the possible presence of coevolutionary patterns between this leech and its turtle hosts is available due to the paucity of DNA sequence data available for P. costata; moreover, comparative host data is also mostly lacking, making any inferences more difficult. The discovery of new populations of the species in northern Italy and Sicily allowed us to generate novel mitochondrial DNA sequences and to compare the topology of the resulting phylogenetic trees with the phylogeny of the turtle hosts occurring in the study are…

Strong reproductive barriers in a narrow hybrid zone of West-Mediterranean green toads (Bufo viridis subgroup) with Plio-Pleistocene divergence

Abstract Background One key question in evolutionary biology deals with the mode and rate at which reproductive isolation accumulates during allopatric speciation. Little is known about secondary contacts of recently diverged anuran species. Here we conduct a multi-locus field study to investigate a contact zone between two lineages of green toads with an estimated divergence time of 2.7 My, and report results from preliminary experimental crosses. Results The Sicilian endemic Bufo siculus and the Italian mainland-origin B. balearicus form a narrow hybrid zone east of Mt. Etna. Despite bidirectional mtDNA introgression over a ca. 40 km North-South cline, no F1 hybrids could be found, and nu…

Climate change and population genetic structure of marine species

Climate change influences populations by reducing or extirpating local populations, by disrupting patterns of migration and by shifting geographical distributions. These events can affect genetic population structure in several ways. Molecular markers have been used in numerous population genetic and phylogeographical studies of marine species and have detected population responses to climate change in the last few decades, such as range expansions, adaptative shifts and declines or increases in abundance. Little is known, however, about the molecular and physiological basis of adaptive responses to climate change in marine Mediterranean species. The Mediterranean Sea ecosystem is a ‘living…

Genetic structure of Pomatoschistus marmoratus (Gobiidae) in Mediterranean Sea

Can phthalates move into the eggs of the loggerhead sea turtle Caretta caretta? The case of the nests on the Linosa Island in the Mediterranean Sea

During the monitoring of Caretta caretta nests on the island of Linosa, 30 unhatched eggs from four nests were collected to study the presence of phthalates in their three components (shell, yolk, and albumen). Four phthalates, namely diethyl (DEP), dibutyl (DBP), di-(2-ethylhexyl) (DEHP), and dioctyl (DOTP) phthalic acid esters (PAE), which are widely used as additives in plastics, were detected in all egg components. The most frequently found phthalate was DBP, followed by DEHP in eggshell and yolk. Dimethyl- (DMP) and butylbenzyl-phthalate (BBP) were below the limits of detection for all samples. The high total phthalate recorded in the yolk suggests that contamination could arise by vit…

First record of the North American cryptic invader Ferrissia fragilis (Tryon, 1863) (Mollusca: Gastropoda: Planorbidae) in the Middle East

Some gastropod specimens belonging to the planorbid genus Ferrissia were recently collected in Lebanon and in Iraq, where the autochthonous species Ferrissia clessiniana (Jickeli, 1882) is supposed to occur. The molecular identification of collected specimens proved that they belong to the allochthonous species Ferrissia fragilis (Tryon, 1863), the protagonist of a dramatic cryptic invasion which is of interest to the whole of Eurasia. These findings cast further doubts on the actual existence of autochthonous Ferrissia species in the Palaearctic. The need for a molecular characterisation of the topotypical population of F. clessiniana, and for a revision of the Palaearctic Ferrissia specie…

COME E' DISTRIBUITA LA BIODIVERSITA' IN MEDITERRANEO E PERCHE': LA RISPOSTA DEGLI APPROCCI MOLECOLARI

Italian Peninsula preserves an evolutionary lineage of the fat dormouse Glis glis L. (Rodentia: Gliridae)

The present study examines the population genetic structure of fifty-nine specimens of Glis glis (Linneaus, 1766) from thirteen localities in central Europe, sequencing a 400-bp segment of the mitochondrial cytochrome b (cyt b) gene and a 673-bp segment of the cytochrome c oxidase subunit I (COI) gene. The consensus tree obtained from Bayesian analysis revealed a robust dichotomy, showing two sister groups: one clade includes samples from a wide geographical area, extending from north-central Europe to northern Italy (major branch sensu Bilton), and the other comprises samples collected in central and southern Italy and in Sicily (Italian branch). According to the Tajima–Nei model, the two …

Environmental features of deep-sea habitats linked to the genetic population structure of a crustacean species in the Mediterranean Sea

The deep-sea habitat, from 200 to 2000 m depth, has long been thought as an ecosystem where biotic and abiotic factors vary very little and consequently species are not disturbed by processes and phenomena which could promote fast evolutionary mechanisms. Unfortunately, biological information relating to deep water is limited, especially regarding the population genetics of species inhabiting the Mediterranean Sea, and general patterns cannot be inferred. In this study we report data on the population genetic structure of Aristeus antennatus, a deep-sea decapod crustacean species which has been widely studied due to its important economic value. We surveyed and examined the variation in a 3…

Overlapping patterns of morphometric and genetic differentiation in the Mediterranean goby Pomatoschistus tortonesei Miller, 1968 (Perciformes, Gobiidae) in Tunisian lagoons

Abstract. The genetic and morphological variations of Pomatoschistus tortonesei Miller, 1968 were studied in samples collected from three Tunisian lagoons. The morphological analysis included 18 morphometric measurements and was based on linear discriminant analysis (LDA), whereas the genetic analysis was based on the 16S-rRNA and COI mitochondrial genes. Both analyses differentiated the populations and demonstrated consistently a well-supported differentiation between the western Mediterranean samples (Bizerta and Tunis South lagoons) and the eastern Mediterranean sample (El Bibane lagoon). The observed differentiation could be explained in terms of the geographic isolation of the various …

Current Status of and Threats to Sicilian Turtles

Based on the critical review of the literature published in the last 22 years, an attempt was made to evaluate the current knowledge gap on the distribution and status of the native Testudines taxa occurring in Sicily (namely Caretta caretta, Emys trinacris, and Testudo hermanni hermanni), as well as the available knowledge of the only non-native species with putative viable populations occurring on the island, i.e., Trachemys scripta. Summarizing the current information, all of the Testudines species occurring in Sicily showed a fragmented and incompletely-known distribution, and only scarce data are available about their phenology. Moreover, despite their inclusion of international and na…

Unveiling the egg microbiota of the loggerhead sea turtle Caretta caretta in nesting beaches of the Mediterranean Sea

Microbes have central roles in the development and health of animals, being the introduction of specific microbial species a potential conservation strategy to protect animals from emerging diseases. Thus, insight into the microbiota of the species and their habitats is essential. In this manuscript, we report for the first time the bacterial composition of all the components (eggshells of hatched and unhatched eggs, internal content of unhatched eggs, intestinal content of hatchling and pipping sea turtles, and sand) of three nesting beaches of Caretta caretta along the Italian coasts of the Mediterranean Sea. The analysis of 26 amplicon samples was carried out using next-generation sequen…

Genetic architecture of the marbled goby Pomatoschistus marmoratus (Perciformes, Gobiidae) in the Mediterranean Sea

The marbled goby Pomatoschistus marmoratus, a species inhabiting coastal Mediterranean lagoons, has been studied by measuring its mitochondrial DNA variation. This analysis revealed a Mediterranean west vs east split and, subsequently, an eastern differentiation among the Libyan–Tunisian Gulf, the Adriatic Sea and the Aegean Sea. The high cohesion between the samples collected in the vast area of western Mediterranean contrasts with the genetic mosaic of the more sub-structured eastern Mediterranean. This western homogeneity can not yet be fully explained even if a human-mediated migratory flow, due to a maritime traffic, has been posited. The pattern in the eastern basin revealed a genetic…

Foreword to the special issue "new frontiers for monitoring European biodiversity: The role and importance of amphipod crustaceans"

Contribute to the knowledge of Tunisian amphibians: Notes on distribution, habitat features and phenology

DNA Taxonomy Confirms the Identity of the Widely-Disjunct Mediterranean and Atlantic Populations of the Tufted Ghost Crab Ocypode cursor (Crustacea: Decapoda: Ocypodidae)

The distribution area of the tufted ghost crab Ocypode cursor includes two widely separate sub-areas, i.e. the tropical and subtropical Atlantic coasts of Africa and Macaronesia, and the central-eastern Mediterranean basin. The current disjunct distribution of the species is possibly the remnant of a previous wider and continuous distribution area that was fragmented during the Pleistocene, with the disappearance of the species from the temperate Atlantic Ocean and the western Mediterranean basin, and its survival in the warmer areas of the eastern Atlantic and the Mediterranean Sea. Such disjunction is thus compatible with an ancient isolation between the Mediterranean and Atlantic populat…

Ecomorphological variation within and among the two marine species of genus Salaria: Salaria basilisca and Salaria pavo (Perciformes: Blenniidae)

In order to highlight the variation of the form due to the presence of different ecological factors, we investigated in this paper the morphological variation between and within the two marine species Salaria basilisca and S. pavo. This study was based on two approaches, the Truss network and the geometric morphometric and involved specimens of both species divided into marine and lagoon samples. The two approaches yielded complementary results showing clear morphological discrimination of the two species Thus, S. basilisca is distinguished by a higher depth and length as well as a higher head with a compressed snout compared to S. pavo which has a shortened body, a lower head and a slightl…

Assessing multiple sources of data to detect illegal fishing, trade and mislabelling of elasmobranchs in Greek markets

Abstract Elasmobranchs, extremely charismatic and threatened animals, still are an important economic source for fishers in many parts of the world, providing significant income through trade. Even though Greek seas host at least 67 elasmobranch species, our knowledge about their biology and ecology is to a large extent unknown. In the present study the integration of conventional (legislation, official data from fisheries landings and fish market value and import/export data) and unconventional (social media) sources of data, accompanied with the use of genetics, aim at outlining the elasmobranch fisheries and trade in Greece and identifying “weak spots” that sabotage their conservation. R…

FISH BARCODE OF LIFE: primi dati sui pesci ossei e pesci cartilaginei del mar Mediterraneo.

Analisi dei dati di sbarco del gambero viola Aristeus antennatus (Crustacea Aristeidae) in due marinerie della Sicilia nord occidentale.

Protein differences among the Mediterranean species of the genus Spicara.

Protein electrophoresis (PAGE) was used to study the three morphologically different species of Spicara (S. flexuosa, S. maena, S. smaris). Of the 28 enzymatic and additional myogenic loci, five monomorphic loci (LDH-1*, G6PD-1*, PGI-1* and two PMMs*) were species-specific markers of S. smaris with respect to S. flexuosa and S. maena. Four of the 28 enzymatic loci were polymorphic (EST-1*, GLDH*, PEPD*, PGI-2*). Discriminating genetic markers were not identified between S. flexuosa and S. maena. Genetic distance (D) as calculated by Nei’s index (1978), between S. smaris v. S. maena and S. flexuosa showed a value, respectively of D=0·137 and 0·141. Between S. flexuosa and S. maena the value …

Recent expansion of Northeast Atlantic and Mediterranean populations of Melicertus (Penaeus) kerathurus (Crustacea: Decapoda).

We analysed the genetic diversity of Melicertus kerathurus (Penaeidae), a commercially valuable penaeid shrimp that is distributed in the Mediterranean Sea and eastern Atlantic Ocean. We examined the polymorphism of a 494 bp DNA segment of the mitochondrial COI region in 173 individuals, sampled in nine Mediterranean and two Atlantic samples, covering the whole range of the species from the tropical waters of the Gulf of Guinea to the eastern part of the Mediterranean Sea. The mean nucleotide and haplotype diversities were π = 0.00275 and h = 0.718, respectively, for the global data set, with the highest values occurring in the African samples and the lowest in the Adriatic Sea. A clear sam…

Morphological variability in and distributional data on phaleria bimaculata populations from islands of the central mediterranean area

This study aimed to collect new taxonomic and distribution data on Phaleria bimaculata (L.) (Coleoptera Tenebrionidae) from Sicily (southern Italy), the circum-Sicilian islands and the Maltese archipelago. Particular attention was paid to the diagnostic morphological features observed in the populations sampled, focusing on the subspecies Phaleria bimaculata marcuzzii Aliquò. P. bimaculata individuals were collected from 20 different locations, of which 2 were located in the Sicilian mainland, 9 in circum-Sicilian islands and 9 in the Maltese Islands. In addition, four Phaleria acuminata Kuster populations were also recorded along southern Sicilian shores. The two Phaleria species were neve…

Pliocene colonization of the Mediterranean by Great White Shark inferred from fossil records, historical jaws, phylogeographic and divergence time analyses

Aim: Determine the evolutionary origin of the heretofore poorly characterized contemporary Great White Shark (GWS; Carcharodon carcharias) of the Mediterranean Sea, using phylogenetic and dispersal vicariance analyses to trace back its global palaeo-migration pattern. Location: Mediterranean Sea. Taxon: Carcharodon carcharias. Methods: We have built the largest mitochondrial DNA control region (CR) sequence dataset for the Mediterranean GWS from referenced historical jaws spanning the 19th and 20th centuries. Mediterranean and global GWS CR sequences were analysed for genetic diversity, phylogenetic relationships and divergence time. A Bayes factor approach was used to assess two scenarios …

The European Pine Marten Martes martes (Linnaeus, 1758) Is Autochthonous in Sicily and Constitutes a Well-Characterised Major Phylogroup within the Species (Carnivora, Mustelidae)

No molecular data are currently available for the Sicilian populations of the European pine marten Martes martes, thus preventing any sound inference about its native or non-native status on the island, as well as the local phylogeography of the species. In order to investigate these issues, we sequenced two mtDNA markers in road-killed specimens collected in Sicily. Both markers consistently demonstrated the existence of a well-characterised Sicilian clade of the species, which is endemic to the island and constitutes the sister group of a clade including the Mediterranean and Central–North European major phylogroups of the European pine marten. Such evidence supports the autochthony of Ma…

Multi-Locus Phylogenetic Analyses of the Almadablennius Clade Reveals Inconsistencies with the Present Taxonomy of Blenniid Fishes

We used a multi-locus phylogenetic approach (i.e., combining both mitochondrial and nuclear DNA fragments) to address some long-standing taxonomic inconsistencies within the diverse fish clade of Combtooth Blennies (Blenniidae—unranked clade Almadablennius). The obtained phylogenetic trees revealed some major inconsistencies in the current taxonomy of Parablennini, such as the paraphyletic status of the Salaria and Parablennius genera, casting some doubt regarding their actual phylogenetic relationship. Furthermore, a scarce-to-absent genetic differentiation was observed among the three species belonging to the genus Chasmodes. This study provides an updated taxonomy and phylogeny of the fo…

L’analisi della regione di controllo mitocondriale rivela due pool genici nella ricciola mediterranea Seriola dumerili lungo le coste siciliane

Origine, diffusione e storia evolutiva del mitile lessepsiano Brachidontes pharaonis (P. Fisher, 1870).

A molecular analysis of some Eastern Atlantic grouper from the Epinephelus and Mycteroperca genus

Abstract Mitochondrial cytochrome b (397 bp) and 16S rDNA (516 bp) sequences analysis was used to investigate the phylogenetic relationships among some Eastern Atlantic Epinephelinae species. Six species of Epinephelus ( E. aeneus , E. caninus , E. costae , E. haifensis , E. marginatus and E. tauvina ) and two species of Mycteroperca ( M. rubra and M. fusca ) were analysed. Neighbour-joining and maximum-parsimony analysis support the paraphyletic grouping of the Epinephelus and Mycteroperca analysed. The maximum pairwise nucleotide divergence value in cyt b among all taxa was 0.196 between E. aeneus and E. marginatus and the minimum value was 0.006 between E. costae and M. rubra . Meanwhile…

DNA barcoding of marine fishes from Saudi Arabian waters of the Gulf

We used the cytochrome oxidase subunit I (coI) gene DNA to barcode 117 endemic Gulf and cosmopolitan Indo-West Pacific fish species belonging to 54 families and 13 orders. Novel DNA barcodes were provided for 18 fish species (Trachinocephalus sp., Nematalosa sp., Herklotsichthys lossei, Upeneus doriae, Trachurus indicus, Apogonichthyoides taeniatus, Verulux cypselurus, Favonigobius sp., Suezichthus gracilis, Sillago sp., Brachirus orientalis, Pegusa sp., Lepidotrigla bispinosa, Lepidotrigla sp., Grammoplites suppositus, Hippichthys sp., Paramonacanthus sp. and Triacanthus sp.). The species delimitation analysis, conducted with Poisson tree processes- Bayesian PTP (PTP-bPTP) and nucleotide-d…

Hatching Success of Caretta caretta on a Mediterranean Volcanic Beach: Impacts from Environmental Parameters and Substrate Composition

New data on the potential impact of environmental parameters and the mineralogical nature of the substrate on the hatching success of the loggerhead sea turtle Caretta caretta on a volcanic beach on Linosa Island in the central Mediterranean Sea are reported. During 2 years of investigation (2018-19), five nests were observed. The temperature and moisture of the nests were determined along with the grain size and mineralogical nature of the substrate. The temperature values of the nests recorded at depths of 5 and 35 cm showed an average temperature incubation of 32.2°C ± 1.4°C and 32.8°C ± 1.6°C, respectively, with peaks close to 37°C. The sedimentological analysis carried out on two cores…

Identificazione di stock mediante analisi del DNA nucleare e mitocondriale nelle specie ittiche Merluccius merluccius (nasello), Mullus barbatus (triglia di fango) e Seriola dumerili (ricciola) catturate lungo le coste siciliane.

Molecular systematics and spatial genetic structure of Phaleria bimaculata (Coleoptera: Tenebrionidae) populations from central Mediterranean sandy beaches.

Sandy beaches

Genetic variability of the blue and red shrimp Aristeus antennatus in the Western Mediterranean Sea inferred by DNA microsatellite loci

Genetic variation at eight microsatellite loci was studied in nine populations of the blue and red shrimp Aristeus antennatus to investigate whether distinct stocks are present in the Western Mediterranean Sea. A high level of gene flow and no evidence of genetic partitioning were discovered. No significant variation was found (FST = 0.00673, P-value = 0.067) even when shrimps from exploited and those from deep-water unexploited grounds were compared. No evidence of reduction or expansion of population size in the recent past was found, as indicated by the bottleneck and interlocus g-tests. Our results are consistent with previous studies using mitochondrial gene methods and allozymes, indi…

USO DI MARCATORI MOLECOLARI PER LO STUDIO DELLA VARIAZIONE GENETICA DI MELICERTUS KERATHURUS (CRUSTACEA, DECAPODA)

Diversità genetica e pattern filogeografici in Meuremys leprosa (Testudines, Geoemydidae)

Nonmodal scutes patterns in the Loggerhead Sea Turtle (Caretta caretta): a possible epigenetic effect?

Eleven specimens of the threatened Loggerhead Sea Turtle (Caretta caretta (L., 1758)) were caught accidentally by fishermen in different parts of Sicily (Mediterranean Sea). Five of them showed an atypical number of carapacial and plastron scutes, making the immediate identification of the specimens as C. caretta difficult. Both genetic and epigenetic analysis were carried out on these specimens. Sequencing of a 649 bp sequence of the mitochondrial cytochrome c oxidase I (COI) gene allowed us to classify all the individuals as C. caretta. Epigenetic analysis, performed by evaluating the total level of DNA cytosine methylation, showed a reduced and significant (F = 72.65, p &lt; 0.01) globa…

Gli ambienti acquatici delle reserve naturali orientate “Monte Genuardo e Santa Maria del Bosco”, “Monte Carcaci” e “Monte Cammarata”

First contribute to the systematics and phylogeography of the roubaui-froup of the genus Hemidiaptomus (Copepoda, Calanoida, Diaptomidae).

Italian Peninsula preserves an evolutionary lineage of the fat dormouse Glis glis L. (Rodentia: Gliridae)

Genetic population structure of Epinephelus marginatus (Pisces, Serranidae) revealed by two molecular markers

In this paper, data are presented regarding the population structure of dusky grouper (Epinephelus marginatus) which was sampled in the Mediterranean Sea and Atlantic Ocean using two different molecular markers, ND2 RFLP and cytochrome b (cyt b) sequence analysis. The main objective of the study is to analyse the genetic variability of E. marginatus in the Mediterranean Sea to define how the biological characteristics of the species, such as the dispersal capability of pelagic larvae and the benthonic-sedentary life style, can affect the genetic population structure or maintaining the gene flow or determining genetic differences. Furthermore, we considered an Atlantic sample to establish th…

Genetic divergence between morphological forms of brown troutSalmo truttaL. in the Balkan region of Macedonia

The objective of this study was to characterize the genetic structure of two Balkan brown trout morphotypes, Salmo macedonicus and Salmo pelagonicus, and to test whether molecular traits support the species’ status proposed by traditional morphological identification. The mitochondrial DNA 12S-rDNA, cyt b and control region genes were sequenced in 15 specimens collected from three localities in the Former Yugoslav Republic of Macedonia. The results of these markers did not support the taxonomic category of species but confirmed the existence of two morphotypes, Salmo trutta macedonicus and Salmo trutta pelagonicus, in the Aegean–Adriatic lineages of the Salmo trutta species complex.

Coupling Lagrangian simulation models and remote sensing to explore the environmental effect on larval growth rate: The Mediterranean case study of round sardinella (Sardinella aurita) early life stages

The relationship between environmental conditions and early life-history traits of Sardinella aurita are investigated using material collected in two sites of the Central Mediterranean Sea. Individual mean daily growth during the planktonic phase has been determined by using otolith microstructure analysis, while Lagrangian simulation models allowed to estimate the daily position in space and time of each specimen from the hatching to the catch. Generalized Additive Mixed Models (GAMMs) have been implemented to explore the impact of environmental conditions at time t, t-1 day and t-2 days on the mean daily growth rate occurring at time t. Spatial analysis evidenced a wide dispersion of eggs…

INFLUENZA DELLE CARATTERISTICHE OCEANOGRAFICHE NELLA STRUTTURA DI POPOLAZIONE DI ARISTEUS ANTENNATUS (CRUSTACEA: ARISTEIDAE)

Amendment of the type locality of the endemic Sicilian pond turtle Emys trinacris Fritz et al. 2005, with some notes on the highest altitude reached by the species (Testudines, Emydidae)

The type locality of the Sicilian pond turtle Emys trinacris is here amended, and its correct name and geographical coordinates are provided. The locus typicus of the species lies at 1007 m a.s.l., i.e. nearly 400 m below what previously thought. The updated altitudinal distribution range of the species, based on verified published localities only, is between 0 and 1036 m a.s.l.

Pleistocene diversification in Morocco and recent demographic expansion in the Mediterranean pond turtle Mauremys leprosa

Quaternary climatic oscillations and geographic barriers have strongly influenced the distribution and diversification of thermophilic species occurring in the Mediterranean Basin. The Western Mediterranean pond turtle, Mauremys leprosa, is widely distributed throughout the Iberian Peninsula, southern France and most of the Maghreb region, with two subspecies currently recognized. In this work, we used 566 samples, including 259 new individuals, across the species range, and sequenced two mitochondrial markers (cytochrome b gene and control region; 163 samples in a concatenated mtDNA dataset) and one nuclear intron (R35; 23 samples representing all identified sublineages) to study the evolu…

Range-wide phylogeography and taxonomy of the marine rock pools dweller Tigriopus fulvus (Fischer, 1860) (Copepoda, Harpacticoida)

In the light of the wide distribution and ecological importance of the genus Tigriopus in coastal rock pool habitats, and of its frequent use in aquaculture and as a model organism, we investigated the identity of the Atlantic–Mediterranean Tigriopus populations and elucidated their taxonomy and patterns of morphological and genetic diversity. In order to reach these goals, an “integrative taxonomy” multisource approach was implemented. First, we investigated the constancy and taxonomical value of the morphological characters currently used to distinguish among the Tigriopus species occurring in the Mediterranean and in Eastern Atlantic area north of the Tropic of Cancer, and checked the ac…

Brown trout (Salmo trutta L.) high genetic diversity around the Tyrrhenian Sea as revealed by nuclear and mitochondrial markers

The brown trout (Salmo trutta L.) is widely distributed all around Europe but its natural diversity is threatened by massive stocking with Atlantic domestic strains. Describing the remaining natural genetic diversity and the proportion of domestic hatchery strains in rivers is a prerequisite for smart conservation. The high genetic diversity of brown trout populations around the Tyrrhenian Sea is well known. Use of twelve microsatellites has allowed description of the natural genetic structure of populations and detection of the consequences of stocking. Mitochondrial DNA control region sequences and the LDH-C1* gene enabled placement of each population into one of the six mitochondrial and…

Helminth infestation of three fishes (Serranus scriba, Mullus surmuletus, Scorpaena porcus)from a coastal seaground in the Gulf of Palermo (Tyrrhenian Sea)

Abstract The results of a year's survey on the parasitic infestation of the gastrointestinal tract of three species of benthic fish, namely Serranus scriba, Mullus surmuletus, and Scorpaena porcus, living in a restricted coastal seaground in the N/W sector of the Gulf of Palermo, are reported and critically discussed. Fish populations were sampled monthly by means of a trammel net, according to the local fishing usage. As many as nine helminth species, five of them trematodes and four nematodes, were collected from the gastrointestinal tract of 625 individuals. Infestation was most intense in M. surmuletus, with a peak in the spring. S. scriba was especially parasitized during the spring an…

Stranding patterns of the striped dolphin (Stenella coeruleoalba , Meyen 1833, Delphinidae) along the Sicilian coast (Mediterranean Sea)

An account on the taxonomy and molecular diversity of a marine rock-pool dweller, Tigriopus fulvus (Copepoda, Harpacticoida)

[ES] El género de copépodos Tigriopus Norman, 1869 se distribuye en todo el mundo en charcas de rocas costeras y se considera que actualmente incluye 14 especies válidas. Tigriopus fulvus (Fischer 1860), con sus subespecies Tigriopus fulvus adriaticus Van Douwe 1913 y Tigriopus fulvus algiricus Monard 1935, y Tigriopus minutus Bozic 1960 han sido descritos para el área del Mediterráneo, pero la diversidad real del género es desconocida actualmente. El objetivo de este estudio fue evaluar la identidad real de las poblaciones mediterráneas de Tigriopus y dilucidar su taxonomía y patrón de diversidad genética. Con este fin, se secuenció un fragmento del gen de ADN mitocondrial (citocromo c oxi…

The softh bottom decapod fauna of the Gulf of Castellammare (NW Sicily).

Haemolymphatic Parameters in Two Aquaculture Crustacean Species Cherax destructor (Clark, 1836) and Cherax quadricarinatus (Von Martens, 1868)

In the last few years, there has been a notable development in the breeding of freshwater shrimp (astaciculture), which involved various species and in particular, the two Australian Parastacidae species, Cherax destructor and Cherax quadricarinatus. Information about the haemolymphatic parameters of these two species is fragmentary, and filling these gaps becomes important given their importance in aquaculture. Cellular and biochemical parameters were analyzed in both species to create a reference baseline for these parameters to identify the state of welfare or suffering of these animals. The results showed that the total haemocyte count, haemocyte subpopulations, enzymatic activities and…

The Stock Genetic Structure of Two Sparidae Species, Diplodus vulgaris and Lithognathus mormyrus, in the Mediterranean Sea.

Abstract Polyacrilamide gel electrophoresis (PAGE) of allozymes was used to investigate the intraspecies genetic variation and the genetic stock structure of Diplodus vulgaris and Lithognathus mormyrus captured from eight localities in the Mediterranean Sea. Twenty-two and 20 putative enzyme-coding loci were examined, respectively, in D. vulgaris and L. mormyrus . Polymorphic loci at the 95% level were used to assess the allozyme variability in D. vulgaris ( AAT-2 ∗ , EST-1 ∗ , GLDH ∗ , PEPB-2 ∗ , PGI-2 ∗ , PGM ∗ , SDH ∗ ) and L. mormyrus ( AAT-2 ∗ , EST-1 ∗ , GLDH ∗ , MDH-2 ∗ , PGI-2 ∗ , PGM ∗ ). The proportion of polymorphic loci in both species ranged from 0.31 ( D. vulgaris ) to 0.30 ( …

Haemolymphatic parameters in two aquaculture crustacean species Cherax destructor (Clark, 1836) and Cherax quadricarinatus (von Martens, 1868)

The growing demand for animal proteins of aquatic origin such as fish, molluscs and crustaceans have prompted some European and Italian companies to focus their attention on some species of freshwater crustaceans such as Cherax destructor and Cherax quadricarinatus. They are among the largest freshwater decapods, matures early, females can lay over a thousand eggs in a single brood and has a broad environmental tolerance. All these characteristics make it highly appreciated species for aquaculture and few years ago their breeding has spread to Europe and recently also in Italy (Sicily). Although some commercial yabby farms have been studied these species from different points of view (dieta…

Otolith-based age and growth of the Lessepsian species Fistularia commersonii (Osteichtyes: Fistulariidae) in South of Sicily (Central Mediterranean Sea)

During spring–summer 2013, 23 specimens of the Lessepsian Fistularia commersonii Rüppell, 1838 were studied through occasional catches by artisanal and coastal trawler fisheries in the South of Sicily (Mediterranean Sea). The total lengths (TL, cm, without filament) and weight (W, g) ranged between 69.0 and 104.0 cm and 155 and 798 g, respectively. The estimated sex-combined parameters of the length–weight relationship (k = 9*10–9; b = 3.6) suggested a positive allometry. All pairs of sagittae were examined under a stereomicroscope and the age estimated by counting the complete translucent zones, both as whole and thin sections, with ages from 3 to 5 years. Growth parameters (von Bertalanff…

Brachidontes pharaonis (Bivalvia, Mytilidae), a lessepsian invader from a problematic species-complex

Analisi della struttura genetica di popolazioni siciliane di Merluccius merluccius (Teleostei: Merluccidae) attraverso l’uso di microsatelliti.

Genetic variation pattern in Pomatoschistus tortonesei Miller 1986 from Mediterranean lagoons detected by mitochondrial DNA.

Systematics of European coastal anchovies (genus Engraulis Cuvier).

Reports of morphological differences between European anchovy (Engraulis cf. encrasicolus) from coastal and marine habitats have long existed in the ichthyologic literature, and have given rise to a long-standing debate on their taxonomic status. More recently, molecular studies have confirmed the existence of genetic differentiation between the two anchovy ecotypes. Using ancestry-informative markers, we show that coastal anchovies throughout the Mediterranean share a common ancestry, and that substantial genetic differentiation persists in different pairs of coastal/marine populations despite the presence of limited gene flow. On the basis of genetic and ecological arguments, we propose t…

Microsatellite analysis of red mullet Mullus barbatus (Perciformes, Mullidae) reveals the isolation of the Adriatic Basin in the Mediterranean Sea

AbstractMaggio, T., Lo Brutto, S., Garoia, F., Tinti, F., and Arculeo, M. 2009. Microsatellite analysis of red mullet Mullus barbatus (Perciformes, Mullidae) reveals the isolation of the Adriatic Basin in the Mediterranean Sea. – ICES Journal of Marine Science, 66: 1883–1891. The red mullet Mullus barbatus is commercially one of the most important demersal fish resources in the Mediterranean. Molecular data on its genetic population structure throughout the Mediterranean are reported. Six microsatellite loci displayed a high degree of expected heterozygosity and a high allele number per locus. The Hardy–Weinberg equilibrium test revealed an overall tendency towards heterozygote deficiency, …

Allozyme Similarity in Two Morphologically Distinguishable Populations ofParacentrotus Lividus(Echinodermata) From Distinct Areas of the Mediterranean Coast

Allozymes ofParacentrotus lividusfrom Palermo Gulf in the northern Sicilian coast (Italy) and from a small body-size population in the western Greek coast (Ionian Sea) were investigated by PAGE. Five of the twenty examined loci were polymorphic(AAT*, ADH*, ME*, PGI*andPGM*)over each population with a polymorphism value of 0–25. Average heterozygosity was equal to 0081 in the Sicilian sample and 0084 in the Greek. Deviations from Hardy-Weinberg equilibrium were significant inME*andPGI*loci (as calculated byy).Nei's (1978) genetic distance (D=0–0025) index described a close identity between the two samples. FSTvalue of polymorphic loci ranged from 0001 to 0029, its mean value (0–008) resultin…

First data on microflora of loggerhead sea turtle (Caretta caretta) nests from the coastlines of Sicily (Italy)

ABSTRACT Caretta caretta is threatened by many dangers in the Mediterranean basin, but most are human-related. The purposes of this research were: (i) to investigate microflora in samples from six loggerhead sea turtle nests located on the Sicilian coast and (ii) to understand microbial diversity associated with nests, with particular attention to bacteria and fungi involved in failed hatchings. During the 2016 and 2018 summers, 456 eggs and seven dead hatchling from six nests were collected. We performed bacteriological and mycological analyses on 88 egg samples and seven dead hatchlings, allowing us to isolate: Fusarium spp. (80.6%), Aeromonas hydrophila (55.6%), Aspergillus spp. (27.2%) …

The diaptomid fauna of Israel (Copepoda, Calanoida, Diaptomidae), with notes on the systematics of Arctodiaptomus similis (Baird, 1859) and Arctodiaptomus irregularis Dimentman & Por, 1985 stat. rev

Background: To date, only scarce information is available about the diaptomid copepods of the Middle East despite the ecological and biogeographical importance of the family Diaptomidae in the inland waters of the Holarctic region. Moreover, the taxonomic status of some of the taxa occurring in the area is in need of revision. We studied crustaceans collected from temporary and permanent lentic water bodies in Israel with the aim of providing an updated census of the diaptomid copepods occurring in the country. Furthermore, we morphologically and genetically analysed samples of Arctodiaptomus similis s.l. to shed light on its taxonomy. Results: Five diaptomid taxa were collected during this…

Dati preliminari sulla variabilità della regione di controllo in Parapenaeus longirostris e Aristeus antennatus.

First molecular evidences for the synonimy of Ferrissia wautieri and F. fragilis(MOLLUSCA: PULMONATA: ANCYLIDAE

Although some fossil records attributed to the freshwater limpet genus Ferrissia testify for an ancient occurrence of the genus in Europe, its presence was completely overlooked till the twentieth century, when an increasing amount of records throughout the whole continent peaked out with the description of a new species, Ferrissia wautieri, based on specimens collected in northern Italy. In the last few years, an ever increasing number of records reporting the occurrence of Ferrissia species throughout Europe have been published, but an agreement on the taxonomical identity of the European populations is currently missing. Recent molecular studies aimed at investigating the identity of Pal…

A mitochondrial phylogeography of Brachidontes variabilis (Bivalvia: Mytilidae) reveals three cryptic species

This study examined genetic variation across the range of Brachidontes variabilis to produce a molecular phylogeography. Neighbour joining (NJ), minimum evolution (ME) and maximum parsimony (MP) trees based on partial mitochondrial DNA sequences of 16S-rDNA and cytochrome oxidase (COI) genes revealed three monophyletic clades: (1) Brachidontes pharaonis s.l. from the Mediterranean Sea and the Red Sea; (2) B. variabilis from the Indian Ocean; (3) B. variabilis from the western Pacific Ocean. Although the three clades have never been differentiated by malacologists employing conventional morphological keys, they should be ascribed to the taxonomic rank of species. The nucleotide divergences b…

Identificazione di stock di Mullus barbatus (Teleostei: Perciformes) attraverso l'uso di microsatelliti.

RESTOCKING TRIAL OF MELICERTUS KERATHURUS (DECAPODA, PENAEIDAE) IN THE SHALLOW COASTAL WATERS OF SOUTHWESTERN SICILY (MEDITERRANEAN SEA)

Growth and reproduction data of Plesionika narval (Decapoda, Caridea, Pandalidae) off the Island of Ustica (southern Tyrrhenian Sea)

[We here report some data on the biology and population structure of Plesionika narval (Fabricius, 1787) captured along the coast of the island of Ustica in the southern Tyrrhenian Sea. Specimens were collected monthly from January to October 2000 using commercial traps at depths ranging from 15 to 100 metres. The carapace length (CL) of females ranged between 7.0 and 26.0 mm, and between 7.0 and 17.0 mm for males. Three modes were identified in the cumulative frequency distribution for females but only two for males. In females, the third modal value was observed only at the deeper sample sites. The Von Bertalanffy growth parameters and performance index p were K = 0.65 y–1, CL∞ = 27.4 mm …

Preliminary study on ionic composition in endolymphatic sac of Hoplostethus mediterraneus

An exhaustive phylogeny of the combtooth blenny genus Salaria (Pisces, Blenniidae) shows introgressive hybridization and lack of reciprocal mtDNA monophyly between the marine species Salaria basilisca and Salaria pavo.

A comprehensive phylogeny of the genus Salaria based on mitochondrial and nuclear markers grouped the extant species of the genus in well-characterised marine and freshwater clades, thus rejecting the hypothesis of a polytypic origin of the freshwater Salaria populations and supporting the occurrence of a single invasion event of the inland waters by the genus. Based on both mitochondrial and nuclear DNA datasets, the Salaria species of the freshwater clade proved to be vicariant taxa originating from a common ancestor which could possibly spread throughout the circum-Mediterranean inland waters during the late Miocene Messinian salinity crisis, then experiencing a process of allopatric dif…

Diversity and taxonomy of the genus Unio Philipsson in Italy, with the designation of a neotype for Unio elongatulus C. Pfeiffer (Mollusca, Bivalvia, Unionidae)

Novel Unio spp. populations from Slovenia, the Italian peninsula, Sardinia and Sicily were genetically analysed in order to define the distribution and diversity of the genus Unio in Italy and neighbouring areas. The presence of two primarily allopatric autochtonous species, Unio elongatulus Pfeiffer, and Unio mancus Lamarck, is confirmed for the Italian peninsula, Corsica, Sardinia, and Sicily. Autochthonous populations of Unio elongatulus are present in the peri-Adriatic drainages of the Italian and Balkan peninsulas, south as far as the Ofanto River (Apulia, Italy) and Lake Skadar (Albania), while its presence in the Tyrrhenian rivers of Tuscany is likely due to anthropogenic introductio…

Where are you from, stranger? The enigmatic biogeography of North African pond turtles (Emys orbicularis)

Abstract The European pond turtle (Emys orbicularis) is a Nearctic element in the African fauna and thought to have invaded North Africa from the Iberian Peninsula. All North African populations are currently identified with the subspecies E. o. occidentalis. However, a nearly range-wide sampling in North Africa used for analyses of mitochondrial and microsatellite DNA provides evidence that only Moroccan populations belong to this taxon, while eastern Algerian and Tunisian pond turtles represent an undescribed distinct subspecies. These two taxa are most closely related to E. o. galloitalica with a native distribution along the Mediterranean coast of northern Spain through southern France …

Is Caretta Caretta a Carrier of Antibiotic Resistance in the Mediterranean Sea?

Sea turtles can be considered a sentinel species for monitoring the health of marine ecosystems, acting, at the same time, as a carrier of microorganisms. Indeed, sea turtles can acquire the microbiota from their reproductive sites and feeding, contributing to the diffusion of antibiotic-resistant strains to uncontaminated environments. This study aims to unveil the presence of antibiotic-resistant bacteria in (i) loggerhead sea turtles stranded along the coast of Sicily (Mediterranean Sea), (ii) unhatched and/or hatched eggs, (iii) sand from the turtles&rsquo

Le indagini biomolecolari nell’identificazione di stock ittici lungo le coste siciliane

Chasing phthalates in tissues of marine turtles from the Mediterranean sea

Tissues from thirteen specimens of marine turtles, one Dermochelys coriacea and twelve Caretta caretta, found dead along the Sicilian coasts in 2016 were analyzed for the presence of phthalates. Four phthalates (DEP, DBP, BBP, and DEHP) were found at different significant concentrations in liver and gonads, while only DBP was found in muscle tissues and at a fourfold lower concentration than other phthalates in Dermochelys coriacea. No traces of DEP were detected in C. caretta tissues where DOTP was also revealed. The presence of phthalates in fat tissue in specimens of C. caretta showed a major prevalence of the most lipophilic phthalates DEHP and DOTP. The total concentration of all analy…

Dati di sbarco e biologia del gambero rosa Parapenaeus longirostris (Lucas, 1846) (Crustacea Peneidae) nella Sicilia nord occidentale.

Nuevo registro y caracterización molecular de la tortuga verde Chelonia mydas (Testudines, Cheloniidae) en las aguas costeras sicilianas

A juvenile specimen of the Green Turtle, Chelonia mydas, was recovered in Sicilian coastal waters close to Avola (Syracuse province, south-eastern Sicily, Italy). Before being released, the specimen was measured and inspected for the presence of ingested hooks or other possible harms to its health. A fragment of the mtDNA marker COI was amplified and sequenced in order to provide the first molecular data for the species from the central Mediterranean area. The possible influence of climate change on the occurrence of this thermophilic species in the central Mediterranean area is briefly discussed.

Use of the molecular markers as a tools in fishes stock assessment.

Discriminazione di presunte specie criptiche: un approccio di morfometria geometrica in Brachidontes pharaonis-variabilis (Bivalvia, Mytilidae).

Controlled uptake of PFOA in adult specimens of Paracentrotus lividus and evaluation of gene expression in their gonads and embryos

AbstractPerfluorooctanoic acid (PFOA) has been largely used in the manufacturing industry but a few years ago it turned out to be a dangerous pollutant which is now of concern for terrestrial and aquatic environments. Here, we investigated the bioaccumulation of PFOA in the sea urchin Paracentrotus lividus after exposure to different concentrations of the pollutant for 28 days. We observed rapid uptake of PFOA in the coelomic fluid collected weekly during the exposure period and high bioaccumulation in gonads at the end of the experiment. Interestingly, animals were also able to fast depurate when relocated to a clean environment. In addition, to assess the effect of PFOA on sea urchins’ ph…

Occurrence of the lessepsian species Portunus segnis (Crustacea: Decapoda) in the Gulf of Gabes (Tunisia): First record and new information on its biology and ecology

The alien blue swimming crab, Portunus segnis (Forskal, 1775), an Indo-Pacific species has been recorded in the Southern Mediterranean Sea since decades, even if its occurrence along the Tunisian coasts was not recorded yet. This paper reports the first record of P. segnis in the Gulf of Gabes, south-eastern Tunisia, with few observations on its biology and ecology. Twenty-four females of P. segnis were accidentally caught by local fishermen in October 2014 in shallow sandy areas covered mostly by seagrass and algal beds. Among these 24 individuals, 14 specimens (58.33%) were ovigerous. The mean carapace length (CL) and width (CW) of ovigerous females were 143.0 +/- 5.8 mm and 67.8 +/- 3.6 …

First record of Temnosewellia minor (Platyhelminthes, Temnocephalidae) in Sicily, with a plea for a re-examination of the identity of the publicly available molecular sequences of the genus

Author(s): Vecchioni, Luca; Chirco, Pietro; Bazan, Giuseppe; Marrone, Federico; Arizza, Vincenzo; Arculeo, Marco | Abstract: Ectosymbiotic temnocephalan flatworms belonging to the genus Temnosewellia Damborenea and Cannon, 2001 were collected on Cherax destructor Clark, 1936 in an aquaculture farm in Sicily, Italy. This represents the first record of a temnocephalan species for the fauna of the island. Morphological and molecular identification of the collected specimens proved that they belong to the allochthonous species Temnosewellia minor Haswell, 1888, which was introduced along with crayfishes bred in aquaculture farms. The phylogenetic analyses carried out for the molecular identific…

A study on Pomatoschistus tortonesei Miller 1968 (Perciformes, Gobiidae) reveals the Siculo-Tunisian Strait (STS) as a breakpoint to gene flow in the Mediterranean basin.

The current genetic structure of P. tortonesei, exclusively inhabiting lagoons, may reflect a Plio-Pleistocene colonisation of an ancestor line which has undergone phases of population decline and expansion, following alternate cooling phases. Regarding the calculation of divergence time, it has been estimated that P. tortonesei seems to be the most recent species within the Pomatoschistus genus. It appears also that the discrepancy of P. tortonesei into two distinct phyletic lineages occurred 0.1 Mya. Recurring shifts in sea level and sea surface temperatures of Mediterranean Sea caused the desiccation of shallower lagoons and the consequent bottleneck phenomena of the brackish populations…

Preliminary study on standard protocok for age estimation of teleosts.