Predicting shifting sustainability trade-offs in marine finfish aquaculture under climate change

Defining sustainability goals is a crucial but difficult task because it often involves the quantification of multiple interrelated and sometimes conflicting components. This complexity may be exacerbated by climate change, which will increase environmental vulnerability in aquaculture and potentially compromise the ability to meet the needs of a growing human population. Here, we developed an approach to inform sustainable aquaculture by quantifying spatio-temporal shifts in critical trade-offs between environmental costs and benefits using the time to reach the commercial size as a possible proxy of economic implications of aquaculture under climate change. Our results indicate that optim…

Morphometric analysis of the shells of the Genus Bolivina from the Gulf of Gela (Sicilian Channel, Italy): a valid classification instrument?

New tools to detect the quality of cultured european sea bass Dicentrarchus labrax (L.)

In vivo exposure of the marine sponge Chondrilla nucula Schmidt, 1862 to cadmium (Cd), copper (Cu) and lead (Pb) and its potential use for bioremediation purposes.

Abstract The study aimed to test the cadmium (Cd), copper (Cu) and lead (Pb) bioaccumulation capacity of the marine sponge Chondrilla nucula and to measure the expression of metallothioneins (MTs) by a western blotting assay to explore metal tolerance and its potential use as a bioremediator in contaminated coastal areas. C. nucula was sampled in a protected marine area in order to perform experiments on organisms living in a healthy environment. Under laboratory conditions, the sponges were exposed to increasing concentrations of Cd, Cu and Pb in tanks filled with artificial seawater set up for the experiments. For each metal, three waterborne concentrations (mgL−1) of Cd (0.02–0.04-0.08),…

Decomposition rate and invertebrate colonization of seagrass detritus along a hydrodynamic gradient in a Mediterranean coastal basin: The Stagnone di Marsala (Italy) case study

International audience; Seagrass leaf litter decomposition is a key component of marine carbon flow driven by both biotic and abiotic factors, including water movement. In this study, we analyse Posidonia oceanica litter decomposition and invertebrate colonization in three sites with different hydrodynamics in a coastal basin. Litterbags were put on the sea bed along a gradient of distance from the open sea, implying a different level of water exchange. Leaf litter mass loss and carbon and nitrogen concentration were analysed, and density and biomass of benthic invertebrates colonizing litterbags were recorded after 3, 7, 14, 47, 101, 152 and 221 days. Results showed that in the most shelte…

Preliminary results on the distribution of benthic foraminifera in the lagoon like coastal basin Stagnone di Marsala (Trapani, Italy)

Effetti sulla biodiversità delle attività produttive in mare

Biogeochemical evaluation of historical sediment contamination in the Gulf of Palermo (NW Sicily): Analysis of pseudotrace elements and stable isotope signals

Abstract Sedimentary biogeochemical data from the Gulf of Palermo, which borders one of the major urban and industrial areas of the central Mediterranean, provide a decennial–centennial-scale record of the effects of human activity on this coastal environment. In this study we report trace elements (TE), C org /N tot ratios, δ 13 C org , and δ 15 N tot from dated ( 210 Pb) sediments collected in two coastal stations variably influenced by urban/fluvial loadings and illegal dumping and we compare them with published data for offshore sediments, in order to investigate at a large spatial scale the influence of on-land activities on sedimentary marine system. TE were generally low except for H…

Effetti di input ornitogenici sullo stato trofico di un sistema costiero Mediterraneo (laghetti di Marinello, Sicilia, Italia)

Microbial and meiofaunal response to intensive mussel-farm biodeposition in coastal sediments of the Western Mediterranean

We studied the impact of organic loads due to the biodeposition of a mussel farm in a coastal area of the Tyrrhenian Sea (Western Mediterranean). Sediment chemistry, microbial and meiofaunal assemblages were investigated from March 1997 to February 1998 on monthly basis at two stations: the first was located under the mussel farm, while the second was at about 1-km distance and served as control. Benthic response to changes in the biodepositional regime was investigated in terms of biochemical composition of the sedimentary organic matter, phytopigment content, bacterial abundance and composition and meiofaunal community structure. A large accumulation of chloroplastic pigments, proteins an…

Sensitivity of cholinesterase activities in Ciona intestinalis ascidian to in vitro exposure to organophosphate pesticide azamethiphos

Comparative morphometrics of sharpsnout seabream (Diplodus puntazzo Cetti, 1777), reared in different conditions

The same genetic stock of sharpsnout seabream (Diplodus puntazzo), cultured in different conditions (lot A, monoculture tank; lot B, monoculture offshore cage; lot C, polyculture tank), was analysed using geometric morphometrics in order to detect changes in shape. Rearing in the offshore cage (lot B) had a significant effect on shape variation when compared to rearing in the two enclosed tanks (A and C). Fish from the tanks show a higher frequency (43.7% in lot A and 37.5% in lot C) of the so-called ‘goitred’ and ‘dorsal flat’ forms, their deformations shown by displacements in the corresponding landmarks of the infraopercular and dorsal areas. In contrast, the so-called ‘belly’ fish were …

Fish assemblages cope with ocean acidification in a shallow volcanic CO2 vent benefiting from an adjacent recovery area

Shallow CO2 vents are used to test ecological hypotheses about the effects of ocean acidification (OA). Here, we studied fish assemblages associated with Cymodocea nodosa meadows exposed to high pCO2/low pH conditions at a natural CO2 vent in the Mediterranean Sea. Using underwater visual census, we assessed fish community structure and biodiversity in a low pH site (close to the CO2 vent), a close control site and a far control site, hypothesising a decline in biodiversity and a homogenization of fish assemblages under OA conditions. Our findings revealed that fish diversity did not show a unique spatial pattern, or even significant relationships with pH, but correlated with seagrass leaf …

Meristic character analysis and skeletal anomalies during growth in reared sharpsnout seabream

Sharpsnout seabream (Diplodus puntazzo) is a species currently reared in Mediterranean fish farms. In order to detect the main types of body deformation in sharpsnout seabream. 120 specimens (60 juveniles and 60 adults) taken from a Mediterranean hatchery were examined. In juveniles and adults non-significant differences were found in the number of vertebrae, dorsal spines and pectoral fin rays. Moreover, the number of anomalies varied with age. The juveniles presented 28 types of anomaly, the adults 25. The most important anomalies for juveniles (in terms of frequency of occurrence) were anomalous neural arch or spine (47%), anomalous hemal arch or spine (43%), fusion of hypurals (67%) and…

Feeding ecology of the sand smelt, Atherina boyeri (Risso, 1810) (Osteichthyes, Atherinidae), in the western Mediterranean: evidence for spatial variability based on stable carbon and nitrogen isotopes

The feeding ecology of the sand smelt Atherina boyeri Risso 1810 (Osteichthyes, Atherinidae) was investigated in a Mediterranean coastal basin (Stagnone di Marsala, Italy) by means of carbon and nitrogen stable isotope ratios. Sampling was carried out seasonally in 1999 in two locations characterised by different depth and vegetal coverage. Throughout the year the sand smelt showed enriched δ 13C and δ 15N values in both sampling locations (δ 13C about −12‰ and δ 15N about 12‰). This result suggests the overall importance of the benthic pathway in the food web leading to A. boyeri. Our isotopic picture is only in partial agreement with stomach content data from the literature. Stomach conte…

Sedimentary record of anthropogenic contaminants (trace metals and PAHs) and organic matter in a Mediterranean coastal area (Gulf of Palermo, Italy)

Abstract The Gulf of Palermo (Italy, Mediterranean Sea) is a contaminated coastal environment with a relatively high influx of unregulated industrial and domestic effluents. Two sediment box-cores were collected at water depths of 100 and 712 m. Samples were analysed for trace metals (As, Cu, Hg, Mn, Ni, Pb), total polycyclic aromatic hydrocarbons (ΣPAHs), organic carbon to total nitrogen (Corg/Ntot) ratios and organic carbon and nitrogen isotopic composition (δ13Corg and δ15Ntot). At the coastal site, trace metal and ΣPAH depth profiles show a clear upcore increase, indicating increasing contamination over the recent past. Concentrations of ΣPAHs, Hg, Pb, Cu, and As appear to be potentiall…

Gli ambienti costieri di transizione come aree di protezione e sviluppo: l'esempio dello Stagnone di Marsala

Analysis of the trophic role of primary producers in Posidonia oceanica ecosystems along the Sicilian coasts (Italy)

Valutazione degli effetti ambientali della maricoltura basata sull’analisi degli isotopi stabili di azoto

Exploitation of food sources by ichthyofauna in the Acquatina coastal system (Apulia, Italy)

Il Laboratorio di Biologia Marina e Risorse

Trace elements and PAHs in surface sediments and biota from a contamination hot spot (Augusta Bay, Italy): potential export to the adjoining coastal marine areas

Relationships between Suspended and Sediment Organic Matter in a Semi-Enclosed Marine System: The Stagnone Di Marsala Sound (Western Sicily)

To gather information on the interactions between the sediment and suspended organic matter pools in the Stagnone di Marsala, water and sediment samples were collected, on a monthly basis, at 11 stations. Water temperature and salinity showed a clear seasonality whilst particulate and sediment organic matter did not show any clear seasonal pattern. Relative abundances of suspended and sediment organic matter, on the other hand, appeared to be site-dependent and controlled mainly by the dynamic balance between resuspension and sedimentation. High quantities of both suspended and sediment total organic matter were present, while very low algal biomasses (in terms of chlorophyll-a concentratio…

Studio della dinamica di decomposizione del detrito fogliare di Posidonia oceanica lungo un gradiente di confinamento in un’area siciliana a caratteristiche lagunari (Stagnone di Marsala)

The role and contribution of the seagrass Posidonia oceanica (L.) Delile organic matter for secondary consumers as revealed by carbon and nitrogen stable isotope analysis

The δ13C and δ15N values of primary producers and consumers were studied to obtain information on the trophic role of Posidonia oceanica L. Delile, the dominant primary producer, in a Mediterranean shallow environment (the Stagnone di Marsala, western Sicily). δ13C strongly discriminated between pelagic and benthic pathways, with the former based on phytoplankton and the latter on a mixed pool of seagrass detritus, epiphytes and benthic algae as carbon sources. A particularly important trophic role appears to be performed by the vegetal epiphytic community on seagrass leaves (δ13C = -14.9 ± 0.1‰), which supports most of the faunal seagrass community (i.e. Amphipoda, Isopoda, Tanaidacea; δ13…

Sediment features, macrozoobenthic assemblages and trophic relationships (δ13C and δ15N analysis) following a dystrophic event with anoxia and sulphide development in the Santa Giusta lagoon (western Sardinia, Italy)

Macrozoobenthic assemblages and stable carbon (delta C-13) and nitrogen (delta N-15) isotope values of various primary producers (macroalgae and angiosperms) and consumers (macroinvertebrate filter/suspension feeders, deposit feeders, detritivores/omnivores and carnivores and fishes) were studied in the Santa Giusta lagoon (Sardinia, Italy) before (spring) and after (autumn) a dystrophic event which occurred in the summer of 2004. A few days after the dystrophy, the physico-chemical characteristics of sediments and macrozoobenthic assemblages were also investigated. In the latter occasion, high total organic carbon (3.9%) and organic matter (15.9%) contents of surface sediments went togethe…

Caratteristiche ecologiche, fattori di pressione antropica e sviluppo sostenibile di un ambiente costiero mediterraneo (Stagnone di Marsala, Sicilia occidentale)

Particulate Organic Matter Composition in A Semi-Enclosed Marine System

Spatial and temporal changes in the biochemical composition of particulate organic matter in a semi-enclosed marine system (Stagnone di Marsala, Mediterranean Sea) were studied, on a monthly basis, from January to December 1994, in order to assess nutritional value of suspended particles for benthic suspension feeders. According to previous findings, the study site displayed a strong oligotrophy. Chlorophyll-a accounted for a very low fraction of the total suspended matter pool (0.1%), whereas at least 75% of POC was of detrital/heterotrophic origin. POC: PON ratio values indicate that bacterioplankton biomass accounted for a significant fraction of the total POC pool, displaying values com…

Sources and transfer of organic matter in food webs of a Mediterranean coastal environment: Evidence for spatial variability

Abstract The spatial variability in the food web structure of a Mediterranean semi-enclosed coastal environment (Stagnone di Marsala, Italy) was investigated using stable carbon and nitrogen isotopes. Organic matter sources and consumers were sampled in two locations with different environmental features (e.g. hydrodynamic regime, open-sea influence, vegetal coverage). Overall more 13 C-enriched and 15 N-depleted values were found in the central location than in the southern for organic matter sources and consumers. Pelagic consumers (zooplankton and juveniles of transient fish) showed slight spatial differences and in both locations seemed to depend on phytoplankton as the ultimate energy …

The use of very high resolution images for studying Posidonia oceanica reefs

Posidonia oceanica is an endemic Mediterranean seagrass that forms wide and dense meadows from the surface up to about 40 m depth. This species can develop a biogenic structure called matte, a typical terraced formation built up by itself, consisting of intertwined rhizomes, roots and sediment, which may allow shoots to reach the sea surface forming reefs (récif barrière), considered natural monuments. Posidonia oceanica reefs are particularly exposed to the ongoing increase in temperature and in storm frequency and intensity due to climate change, with negative repercussions on their conservation. Much more attention must be paid to the implementation of monitoring tools able to detect ear…

Trace elements and stable isotopes in penguin chicks and eggs: A baseline for monitoring the Ross Sea MPA and trophic transfer studies.

Multi-tissue trace elements (TEs), C, N concentrations and stable isotopes (δ13C, δ15N) of chick carcasses and eggs of Adelie and Emperor penguins were studied to i) provide reference data before the recent institution of the Ross Sea Marine Protected Area (Antarctica), and ii) provide conversion factors that allow estimating C, N, δ13C and δ15N in edible tissues from non-edible ones, thus improving the use of stable isotopes in contamination and trophic transfer studies. Higher concentrations of As, Cd, Cr, Cu, Hg, Mn and Pb were found in chick carcasses than in eggs, suggesting increasing contamination in recent decades and high toxicity risks for penguin consumers. Isotopic conversion fa…

Indicatori dell’impatto ambientale in acquacoltura

Characterization of mitotic chromosomes of four species of the genus Diplodus: Karyotypes and chromosomal nucleolar organizer region phenotypes

Karyotypes have been described in four Mediterranean species of the genus Diplodus (Teleostei, Sparidae), D. vulgaris, D. puntazzo, D. sargus and D. annularis. Chromosomes were mainly acrocentric in all but D. vulgaris, where certain chromosome pairs were subtelocentric. A remarkable intraspecific heteromorphism in the number of NOR-bearing chromosomes along with a substantial interspecific variability in position of chromosomal Ag signals have been encountered. The presumed origin of multiple NOR-bearing chromosomes in Diplodus species and variation of the NOR location is discussed. ? 1996 The Fisheries Society of the British Isles

Premature aging in bone of fish from a highly polluted marine area

Fish species have attracted considerable interest in studies assessing biological responses to environmental contaminants. In this study, the attention has been focussed on fishbone of selected fish species from a highly polluted marine area, Augusta Bay (Italy, Central Mediterranean) to evaluate if toxicant elements had an effect on the mineralogical structure of bones, although macroscopic deformations were not evident. In particular, an attempt was made to evaluate if bone mineral features, such as crystallinity, mineral maturity and carbonate/phosphate mineral content, determined by XR-Diffraction and FT-IR Spectroscopy, suffered negative effects due to trace element levels in fishbone,…

Variazioni spaziali delle concentrazioni di metalli pesanti nei sedimenti di un’area portuale della Sicilia orientale

A biochemical study of the effects of tributyltin on unfertilized eggs, embryos and larvae of the sea squirt Ciona intestinalis

We have used unfertilized eggs, embryos and larvae of the sea squirt Ciona intestinalis (Ascidiacea, Urochordata) to study the effects of tributyltin (TBT) on acetylcholine esterase (AChE) activity. Our results showed an increase of AChE enzyme activity levels after TBT exposure in both unfertilized eggs and embryos. The effects of TBT were more pronounced during the gastrula stage, while no differences between exposed larvae and controls were observed. A mechanism of TBT action on enzyme activity during ascidian embryonic development and the possibility of employing AChE in C. intestinalis as a biomarker of TBT exposure are suggested and discussed.

Are there any distinct genetic sub-populations of sand smelt, Atherina boyeri (Teleostei: Atherinidae) along Italian coasts? Evidence from allozyme analysis

Two hundred and ninety-nine specimens of Atherina boyeri, collected from five Mediterranean sites (three from the open sea (Chioggia, Catania and Gaeta) and two from river mouths (the Birgi and S. Bartolomeo rivers) were analyzed in order to confirm previous genetic studies. Eigtheen loci were scored from 12 enzymatic systems, 3 of which were polymorphic: EST*, PGM* and PGI*. Mean Fst value (0.300, p< 0.05) indicated a high genetic heterogeneity. Pairwise Fst and genetic distance revealed that samples from the river mouths (Birgi and S. Bartolomeo) were grouped separately from the open sea samples (Catania, Chioggia and Gaeta). These results are in agreement with the most recent classificat…

Comparative analyses of chemical, trophic and microbiological variables in sediments from an Eastern Sicilian port: differences in spatial accumulation

Borer polychaetes in seagrass sheaths: a shelter or a trophic relationship? Preliminary results based on carbon and nitrogen stable isotopes

Different key roles of mesoscale oceanographic structures and ocean bathymetry in shaping larval fish distribution pattern: A case study in Sicilian waters in summer 2009

Fish larvae data collected in year 2009 were used to examine the effects of particular environmental conditions on the structure of larvalassemblages in two oligotrophic Mediterranean areas (the Southern Tyrrhenian Sea and the Strait of Sicily). For this purpose, relationships with environmental variables (temperature, salinity and fluorescence), zooplankton biomass, water circulation and bathymetry are discussed. Hydrodynamic conditions resulted very differently between two study areas. The Southern Tyrrhenian Sea was characterized by moderate shallow circulation compared to the Strait of Sicily. In this framework, distribution pattern of larval density in the Tyrrhenian Sea was mainly dri…

Assessing polycyclic aromatic hydrocarbons (PAHs) bioaccumulation in the polychaete Sabella spallanzanii (Gmelin, 1971) (Sabellidae) in Sicilian harbours

Effects of fish farming waste to sedimentary and particulate organic matter in a southern Mediterranean area (Gulf of Castellammare, Sicily): a multiple stable isotope study (δ13C and δ15N)

Abstract Carbon and nitrogen stable isotope analysis was used to investigate the dispersion area of waste material coming from fish farming activities in the western Mediterranean. Tests were conducted to see if uneaten feed and faecal material isotopic signals, originating from fish farms, could be detected in particulate organic matter (POM) and sedimentary organic matter (SOM). The detectable dispersion distance (from under cages as far as 1000 m) of cage-derived organic material was also examined. To do this, carbon (δ13C) and nitrogen (δ15N) composition in POM and SOM collected around the cages, in some control areas and in the waste material, was measured. Mean POM δ13C was −22.9±0.2‰…

Meiofauna as indicator for assessing the impact of fish farming at exposed marine site

This study aimed to detect the impact of organic loads due to biodeposition from a fish farm in an exposed area of the Strait of Sicily, Mediterranean Sea. Sediment chemistry and meiofaunal assemblages were investigated on a seasonal basis at four stations: two from the impacted area and two control stations. The presence of the cages induced a significant accumulation of proteins, lipids and biopolymeric carbon, resulting in a reduction in meiofaunal density at the impacted stations. Changes in community structure were also evident, as meiofauna under the cages were characterized by increased importance of polychaetes and copepods in comparison with a much lower importance of gastrotrichs …

Small-scale variability in geomorphological settings influences mangrove-derived organic matter export in a tropical bay

Abstract. Organic matter (OM) exchanges between adjacent habitats affect the dynamics and functioning of coastal systems, as well as the role of the different primary producers as energy and nutrient sources in food webs. Elemental (C, N, C : N) and isotope (δ13C) signatures and fatty acid (FA) profiles were used to assess the influence of geomorphological setting in two climatic seasons on the export and fate of mangrove OM across a tidally influenced tropical area, Gazi Bay (Kenya). The main results indicate that tidal transport, along with riverine runoff, plays a significant role in the distribution of mangrove organic matter. In particular, a marked spatial variability in the export of…

and variability in Posidonia oceanica associated with seasonality and plant fraction

Abstract The carbon and nitrogen isotopic compositions of fractions of the seagrass Posidonia oceanica (L.) Delile in a Mediterranean shallow environment (Stagnone di Marsala, western Sicily) were investigated seasonally throughout 1998. The stable isotope ratios of seagrass leaves (intermediate and adult), rhizomes, leaf litter and aegagropiles were compared over 1 year in order to distinguish between seasonal and plant part variability. Significant differences in the isotopic composition tested using ANOVA were observed as a function of both plant fraction and season. There was an overall trend towards less discrimination against 13 C in summer (average δ 13 C ∼ −10‰) than in winter (aver…

Meristic variation and skeletal anomalies of wild and reared sharpsnout seabream juveniles (Diplodus puntazzo, Cetti 1777) off coastal Sicily, Mediterranean Sea

Serie storiche della composizione isotopica (δ13C e δ15N) di Posidonia oceanica L. (Delile) in un’area interessata da emissioni idrotermali

Contaminazione da metalli in tracce in un sistema costiero Mediterraneo (laghetti di Marinello, Sicilia, Italia) e loro trasferimento lungo la rete trofica.

Food web structure in Mediterranean coastal lagoons under different trophic conditions

Analysis of the trophic structure in zones with different levels of protection within the Marine Protected Area “Isola di Ustica” based on carbon and nitrogen stable isotopes

Stable isotopes: a tool for understanding trophodynamic functioning in coastal lagoons

Biochemical genetic differentiation between Pomatoschistus marmoratus and P. tortonesei

Several diagnostic genetic markers were identified in Pomatoschistus marmoratus and P. tortonesei using polyacrylamide gel electrophoresis (PAGE) of allozymes. Twenty-one loci were resolved, including the electrophoretic pattern of muscle proteins. The MDH*, PGM1,2*, EST-1,2*, FUM* and PGI-2* loci exhibited different alleles which were fixed for the two species being analysed. Genetic distance, as calculated by Nei's index, showed a value of 0.413. Environmental hypersalinity, could have influenced the geographical distribution of P. tortonesei.

The influence of high pCO2 on otolith shape, chemical and carbon isotope composition of six coastal fish species in a Mediterranean shallow CO2 vent

Naturally acidified environments, such as CO2 vents, are important sites to evaluate the potential effects of increased ocean acidification on marine ecosystems and biota. Here we assessed the effect of high CO2/low pH on otolith shape and chemical composition of six coastal fish species (Chromis chromis, Coris julis, Diplodus vulgaris, Gobius bucchichi, Sarpa salpa, Symphodus ocellatus) in a Mediterranean shallow CO2 vent. Taking into consideration the major and trace elements found near the vent and the gradient of dissolved inorganic carbon, we compared the otolith chemical signatures of fish exposed long-term to elevated CO2 emissions and reduced pH (mean pH 7.8) against fish living in …

La maricoltura sostenibile nella gestione consapevole della fascia costiera

FEEDING-HABITS OF YOUNG-OF-THE-YEAR GREATER AMBERJACK SERIOLA DUMERILI (RISSO, 1810) ALONG THE N/W SICILIAN COAST

A survey was carried out from July to December 1992 in the Gulf of Castellammare (N/W Sicily) in order to study the feeding habits of juveniles of the greater amberjack Seriola dumerili. Stomach content analysis showed three different phases of predation during this period, depending on the size variation of the specimens. Cluster analysis suggested prey choice differed within three size groups. Class I (individuals up to 80 mm SL) feed mainly on the zooplankton community (Copepoda and Crustacea larvae). Class II (individuals ranging from 80 to 120 mm SL) represent a transition stage in which fish continue to feed on zooplankton, but benthic and nectonic items also become important. Class I…

Spatial variations in habitat use and food habits of juvenile and adult fishes in a mangal ecosystem (Gazi Bay, Kenya)

Analysis of stable carbon and nitrogen isotopes as a tool for assessing the environmental impact of aquaculture: a case study from the western Mediterranean

Stable carbon and nitrogen isotope ratios ( 13C/ 12 C and 15N/14 N) of in situ dominant primary producers and consumers were investigated to assess the impact on a natural ecosystem of a land-based fish farm along the south-western coast of Sicily (Italy). The putatively impacted area close to the farm along outfall was compared with other locations at increasing distances from the outfall. Carbon and mainly nitrogen stable isotopes revealed evidence of widespread aquaculture waste in the study site: benthic organisms collected in the impacted area showed more enriched δ 15N signatures than those at greater distances from the outfall, suggesting a large contribution of aquaculture waste to …

Effect of explosive shallow hydrothermal vents on δ13C and growth performance in the seagrass Posidonia oceanica

1. Explosive volcanic submarine activity is expected to affect seagrass communities due to sudden and dramatic changes in the physical and chemical features of sea water and sediments, with possibly large ecosystem effects. However, seagrass response to the harsh environmental conditions that arise due to explosive volcanism is as yet unexplored as it is not easy to predict when and where an eruption will occur. Here, we investigate the uptake of hydrothermal carbon within the seagrass Posidonia oceanica by the analysis of d13C and growth rates in tissue laid down before and after an exceptional and massive hydrothermal gas release in the Aeolian Islands (Italy, Mediterranean Sea). 2. Hydro…

Contributo allo studio di un’area di nursery costiera attraverso la tecnica degli isotopi stabili di carbonio e azoto

Predictive mechanistic bioenergetics to model habitat suitability of shellfish culture in coastal lakes

Quantitative tools based on mechanistic modelling of functional traits able to enhance the sustainability of aquaculture and most other human activities (i.e. reducing the likelihood of detrimental impacts optimising productions), are especially important factors in the decision to site aquaculture facilities in coastal lakes, ponds and lagoons and, in the case of detrimental impact, to adopt mitigation measures. We tested the ability of mechanistic functional trait based models to predict life history traits of cultivable shellfish in shallow coastal lakes. Dynamic Energy Budget (DEB) models were run to generate spatially explicit predictions of Mytilus galloprovincialis life history (LH) …

Dinamica del detrito fogliare fanerogamico lungo un gradiente di confinamento in un’area costiera a caratteristiche lagunari

Trace element storage capacity of sediments in dead P. oceanica mat from a chronically contaminated marine ecosystem

Posidonia oceanica mat is considered a long-term bioindicator of contamination. Storage and sequestration of trace elements and organic carbon (Corg) were assessed in dead P. oceanica mat and bare sediments from a highly polluted coastal marine area (Augusta Bay, central Mediterranean). Sediment elemental composition and sources of organic matter have been altered since the 1950s. Dead P. oceanica mat displayed a greater ability to bury and store trace elements and Corg than nearby bare sediments, acting as a long-term contaminant sink over the past 120 yr. Trace elements, probably associated with the mineral fraction, were stabilized and trapped despite die-off of the overlying P. oceanica…

A study of the pattern of digestive enzimes in Diplodus puntazzo (Cetti, 1777) (Osteychthyes, Sparidae): evidence for the definition of nutritional protocols

The digestive enzymes (proteases, carbohydrases and lipases) present along the alimentary tract of juveniles and adults of Diplodus puntazzo were studied. The data obtained showed a different distribution of the enzymatic activities in the different parts of the alimentary tract. Levels of enzymatic activity in the adults were higher than those measured in juveniles, suggesting that adults have a greater ability to digest larger pieces of food. The enzymatic pattern of D. puntazzo justifies its omnivorous habit and suggests an high potential for digesting vegetable polysaccharides. The results of this study suggest the need to adapt the diet to the digestive potential of this new farming sp…

Mixed Function Oxidase Activity and Organochlorine Levels in Farmed Sharpsnout Seabream (Diplodus puntazzo) from Two Intensive Aquaculture Facilities

Mixed function oxidase (MFO) activities and organochlorine levels were investigated in liver of farmed sharpsnout seabream bred in two intensive facilities, a sea farm and a land-based farm. The aim of this study is to investigate how different facility locations and breeding conditions might affect fish metabolic capabilities and accumulation of toxic chemicals in farmed sharpsnout seabream. The differences between breeding two or more fish species in the same cage or tank known as polyculture, and monoculture (1 species) were also investigated. The results showed that both facility location (sea and land) as well as breeding systems (polyculture and monoculture) might be responsible for t…

First Data on the Mysid Community in the “Stagnone di Marsala” (Western Sicily)

First data on the spatial and temporal distribution of the mysid community in the “Stagnone di Marsala” (western Sicily) from January to December 1996 are reported. The assemblage consists mainly of five species which are common in the Mediterranean Sea: Diamysis bahirensis (55.2%), Siriella armata (18.6%), S. clausii (15.8%), Mysidopsis gibbosa (9.8%) and Mesopodospis slabberi (0.6%). Population structure is described for each species and the reproductive period inferred. A sharp increase in abundance occurred in the spring, while the minima occurred during autumn-winter. The D. bahirensis dominated from March to October and S. clausii in the winter. A large difference, which was mainly qu…

Seasonal patterns of biodiversity in Mediterranean coastal lagoons

Aim: Understanding and quantifying the seasonal patterns in biodiversity of phyto- benthos, macro-zoobenthos and fishes in Mediterranean coastal lagoons, and the species dependence upon environmental factors. Location: The study was carried out in the “Stagnone di Marsala e Saline di Trapani e Paceco,” the largest coastal lagoon system in the central Mediterranean Sea (Sicily, Italy), a Special Protection Area located along one of the central ecological corridors joining Africa and Europe. Methods: The coastal lagoon system was selected as a model ecosystem to investi- gate the seasonal variations in biodiversity indices and dominance–diversity relation- ships in phytobenthos, macro-zoobent…

Tracking multiple pathways of waste from a northern bluefin tuna farm in a marine-coastal area.

Aquaculture of bluefin tuna in Mediterranean coastal waters has generated growing concern about the negative environmental effects. In the present isotopic study we examined the dispersal and fate of organic matter derived from a Mediterranean tuna farm in the surrounding environment. An overall enrichment in the heavy nitrogen isotope was found in the feed and in farmed tunas, indicating the input of isotopically traceable organic matter in the system. Waste was clearly traceable in the water column up to 1000 m from the cages, while only slight accumulation occurred in the sediment just below the cages. Waste was isotopically shown also to contribute to the diet of demersal and benthopela…

Dati preliminari sulle associazioni a Foraminiferi Bentonici del Golfo di Priolo (Sr) - Sicilia Sud-Orientale

Diet and habitat use influence Hg and Cd transfer to fish and consequent biomagnification in a highly contaminated area: Augusta Bay (Mediterranean Sea)

Abstract Total mercury (T-Hg) and cadmium (Cd) were measured in twenty species of fish to study their bioaccumulation patterns and trophodynamics in the Augusta Bay food web. Adult and juvenile fish were caught in 2012 in Priolo Bay, south of the Augusta harbour (Central Mediterranean Sea), which is known for the high trace element and polycyclic aromatic hydrocarbon contamination level. T-Hg concentration was found to significantly increase along δ15N and from pelagic to benthic sedentary fish, revealing a marked influence of trophic position and habitat use (sensu Harmelin 1987) on T-Hg accumulation within ichthyofauna. Cd showed the opposite pattern, in line with the higher trace element…

Pianificazione delle attività per uno sviluppo sostenibile della maricoltura in Sicilia. Modello di orientamento per la mitigazione dell'impatto

Environmental quality and sustainable resource exploitation in marine coastal ecosystems: the case study of tuna farming.

Evidences on alterations in skeleton composition and mineralization in a site-attached fish under naturally acidified conditions in a shallow CO2 vent

Abstract Background Ocean acidification may affect fish mineralized structures (i.e. otoliths and skeleton). Methods Here, we compared the elemental composition of muscle and skeleton and the mineral features of skeleton in the site-attached fish Gobius bucchichi naturally exposed to high pCO2/low pH conditions in a shallow CO2 vent with fish of the same species exposed to normal pH. Results Overall, no skeleton malformations were found in both pH conditions, but among-site differences were found in the elemental composition. Interestingly, higher Ca/P values, inducing a moderate skeleton maturation, were found in fish exposed to acidified conditions than in controls. Conclusion Our finding…

Experiences of integrated mariculture in a southern Tyrrhenian area (Mediterranean Sea)

To ascertain the potential for exploiting marine areas for mariculture, data on the cultivation of molluscs and fish in the open sea of the southern Tyrrhenian were collected from May 1994 to June 1995. The aims of this integrated study were to test simple breeding methods for molluscs and fish, to apply these to the practices employed by local fishermen and to experiment with the use of a cage system requiring a low level of investment. Crassostrea gigas (Thunberg) and Mytilus galloprovincialis (Lamarck) were cultivated on submerged long lines around cages used for cultivating Seriola dumerili (Risso) and Diplodus puntazzo (Cetti). S. dumerili specimens were placed in two cages and fed wit…

Small scale variability of geomorphological settings influences mangrove-derived organic matter export in a tropical bay

Abstract. Organic matter (OM) exchanges between adjacent habitats affect the dynamics and functioning of coastal systems, as well as the role of the different primary producers as energy and nutrient sources in food webs. Elemental (C, N, C : N) and isotope (δ13C) signatures and fatty acid (FA) profiles were used to assess the influence of geomorphological setting in two climatic seasons on the export and fate of mangrove OM across a tidally influenced tropical area, Gazi Bay (Kenya). The main results indicate that tidal transport, along with riverine runoff, play a significant role in the distribution of mangrove organic matter. In particular, a marked spatial variability in the export of …

Bottom-up control on macrobenthic communities in a guanotrophic coastal system.

Soft bottom macrobenthic assemblage and sediment characteristics were studied from autumn 2008 to summer 2009 in three coastal ponds (Marinello ponds, Sicily, Italy) at increasing distances from a gull (Larus michahellis Naumann, 1840) colony to investigate the effect of seabird-induced eutrophication (i.e. guanotrophication) on macrobenthic communities. We hypothesized that enhanced nutrient concentration and organic load caused by guano input significantly alter the sedimentary condition of ponds, affecting benthic fauna through a bottom-up control. Polychaetes were the dominant taxon in the system, followed by amphipods, gastropods and bivalves. Macrobenthic patterns showed high variabil…

Impact on the water column biogeochemistry of a Mediterranean mussel and fish farm

We investigated and compared the impact of organic loads due to the biodeposition of mussel and fish farms on the water column of a coastal area of the Tyrrhenian Sea (Western Mediterranean). Physico-chemical data (including oxygen, nutrients, DOC and particulate organic matter), microbial variables (picoplankton and picophytoplankton density and biomass) and phytoplankton biomass (as chlorophyll-a) were determined on a monthly basis from March 1997 to February 1998. The results of this study indicate that both fish farm and mussel culture did not alter significantly dissolved inorganic phosphorus and chlorophyll-a values, while inorganic nitrogen concentrations were higher in mussel farm a…

δ13C and δ15N variability in Posidonia oceanica associated with seasonality and plant fraction

The carbon and nitrogen isotopic compositions of fractions of the seagrass Posidonia oceanica (L.) Delile in a Mediterranean shallow environment (Stagnone di Marsala, western Sicily) were investigated seasonally throughout 1998. The stable isotope ratios of seagrass leaves (intermediate and adult), rhizomes, leaf litter and aegagropiles were compared over 1 year in order to distinguish between seasonal and plant part variability. Significant differences in the isotopic composition tested using ANOVA were observed as a function of both plant fraction and season. There was an overall trend towards less discrimination against 13C in summer (average δ13C ∼ -10‰) than in winter (average δ13C ∼ -…

Using web-based information systems and stable isotopes analysis to investigate the trophic structure of a fish assemblage in a Mediterranean lagoon

The comparative biological effects of spatial management measures in protecting marine biodiversity: a systematic review protocol

Background: The global decline of marine biodiversity and the perceived need to protect marine ecosystems from irreparable alterations to ecosystem functioning and ecosystem service provision have produced an extensive range of spatial management measures (SMMs). The design of SMMs is a complex process often involving the integration of both conservation objectives and socio-economic priorities and the resultant trade-offs are highly dependent on the management regime in place. Future marine management is likely to involve greater use of different forms of protected areas with differing levels of protection, particularly for sites where there are multiple competing demands. Consequently, ev…

Experiences of integrated mariculture in a southern Tyrrhenian area (Mediterranean Sea)

To ascertain the potential for exploiting marine areas for mariculture, data on the cultivation of molluscs and fish in the open sea of the southern Tyrrhenian were collected from May 1994 to June 1995. The aims of this integrated study were to test simple breeding methods for molluscs and fish, to apply these to the practices employed by local fishermen and to experiment with the use of a cage system requiring a low level of investment. Crassostrea gigas (Thunberg) and Mytilus galloprovincialis (Lamarck) were cultivated on submerged long lines around cages used for cultivating Seriola dumerili (Risso) and Diplodus puntazzo (Cetti). S. dumerili specimens were placed in two cages and fed wit…

Stable carbon and nitrogen ratios in the sand smelt from a Mediterranean coastal area: Feeding habits and effect of season and size

Enriched (δ13C values (annual average - 11·2%) suggest that in the study site (Stagnone di Marsala, Italy) the sand smelt Atherina boyeri exploited benthic prey throughout the year. In particular, Mysidacea and Isopoda seemed to dominate the diet. Stomach content data from the literature was in partial agreement with the present isotopic analysis suggesting that, while Mysidacea and Isopoda are exploited by A. boyeri, Copepoda Harpacticoida and Amphipoda are the dominant food items of the sand smelt diet. Several hypotheses are proposed to explain such partially different results from the two techniques. Both season and size influenced the δ13C and δ15N values of A. boyeri. Although through…

Horizontal and vertical food web structure drives trace element trophic transfer in Terra Nova Bay, Antarctica

Abstract Despite a vast amount of literature has focused on trace element (TE) contamination in Antarctica during the last decades, the assessment of the main pathways driving TE transfer to the biota is still an overlooked issue. This limits the ability to predict how variations in sea-ice dynamics and productivity due to climate change will affect TE allocation in the food web. Here, food web structure of Tethys Bay (Terra Nova Bay, Ross Sea, Antarctica) was first characterised by analysing carbon and nitrogen stable isotopes (δ13C, δ15N) in organic matter sources (sediment and planktonic, benthic and sympagic primary producers) and consumers (zooplankton, benthic invertebrates, fish and …

Seabird influence on ecological processes in coastal marine ecosystems: an overlooked role?

Coastal marine ecosystems and especially transitional environments host a large variety of birds, functioning as nesting sites for resident species and corridors for migratory ones. Seabirds activity may trigger movement of nutrients and organic matter between coastal boundaries, affecting their availability and processing. Consequently seabirds are often labelled as biotic vectors. While attention has been focused on the study of biodiversity level and migratory patterns of avifauna, there is little information on the effects of seabirds on ecological features (i.e. trophic status, biodiversity level, food web patterns, transfer of contaminants) in coastal marine ecosystems. To address the…

Studio del comportamento alimentare di Sparus aurata selvatico e allevato in cattività nei confronti di Cerastoderma glaucum

The effects of anthropogenic organic matter inputs on stable carbon and nitrogen isotopes in organisms from different trophic levels in a southern Mediterranean coastal area.

Stable isotope ratios were used to determine the impact of anthropogenically derived organic matter from onshore and offshore fish farming and a sewage outfall on organisms at different trophic levels (primary producers and consumers) on the south-east coast of Sicily (Italy, Mediterranean). Representative macroalgae and consumers were collected in three sampling locations: "Impact" and two putative "Controls" sited to the north of the impacted location. While delta(13)C values of both organic matter sources and consumers varied little between locations, delta(15)N spatial variability was higher and delta(15)N was shown to be a good descriptor of organic enrichment and uptake of anthropogen…

The role of seagrasses as biological barriers against riverine anthropic input in coastal marine ecosystems

Element-specific behaviour and sediment properties modulate transfer and bioaccumulation of trace elements in a highly-contaminated area (Augusta Bay, Central Mediterranean Sea)

Abstract High sediment contamination in the coastal area of Priolo Bay, adjacent to the highly-polluted Augusta Harbour, poses serious risks for the benthic communities inhabiting the area. Nevertheless, the transfer of trace elements and consequent bioaccumulation in the biota is an overlooked issue. This study aimed to assess the transfer and bioaccumulation patterns of As, Cd, Ni and Hg to the dominant macroalgae and benthic invertebrates of Priolo Bay. Results revealed different patterns among trace elements (TEs), not driven by sediment contamination but rather by element-specific behaviour coupled with sediment physicochemical properties. Specifically, As accumulated in macroalgae but…

Conventional karyotype, nucleolar organizer regions and genome size in five Mediterranean species of Syngnathidae (Pisces, Syngnathiformes)

Conventional karyotypes, NOR-bearing chromosomes by means of silver impregnation and genome size were investigated in five Mediterranean species in three genera of the Syngnathidae. A karyotype of 48 subtelocentric-acrocentric chromosomes was found in the seahorse Hippocampus hippocampus (FN=48) while a diploid value of 44 occurred in H. guttulatus (2 sm-m+42 a; FN=46) and the pipefish Syngnathus abaster (44 a; FN=44) and S. typhle (44 a; FN=44). The pipefish Nerophis ophidion, possessing a diploid chromosomal set of 58 made up of 50 meta-submetacentric and eight subteloacrocentric elements (FN=108) and a genome size three to four times larger than those known to date, differs cytogenetical…

Relationships between suspended and sediment organic matter in a semi- enclosed marine system: The Stagnone di Marsala sound (Western Sicily)

To gather information on the interactions between the sediment and suspended organic matter pools in the Stagnone di Marsala, water and sediment samples were collected, on a monthly basis, at 11 stations. Water temperature and salinity showed a clear seasonality whilst particulate and sediment organic matter did not show any clear seasonal pattern. Relative abundances of suspended and sediment organic matter, on the other hand, appeared to be site-dependent and controlled mainly by the dynamic balance between resuspension and sedimentation. High quantities of both suspended and sediment total organic matter were present, while very low algal biomasses (in terms of chlorophyll-a concentratio…

Acque di mezzo. Viaggio alla scoperta degli ambienti costieri della Sicilia: laghi, paludi, pantani, saline e stagni

Effects of a small seagull colony on trophic status and primary production in a Mediterranean coastal system (Marinello ponds, Italy)

Abstract Colonies of seabirds have been shown to influence nutrient cycling and primary production of coastal areas, but knowledge is still limited above all for smaller colonies. This study evaluates the influence of a small resident seagull colony (Larus michahellis Naumann, 1840) on a Mediterranean coastal system (Marinello ponds, Sicily, Italy). The presence of ornithogenic organic matter from seagull guano was first assessed at increasing distances from the colony using δ15N to indicate the effects of guano on the trophic status and primary production. The pond directly affected by guano deposition showed an anomalous water and sediment chemistry, especially regarding physico-chemical …

Effetti della predazione di giovanili di specie ittiche sulla comunità meiobentonica in un ’area di nursery costiera (Castellammare del Golfo,Sicilia Nord Occidentale)

Limited Stress Response to Transplantation in the Mediterranean Macroalga Ericaria amentacea, a Key Species for Marine Forest Restoration

In the Mediterranean Sea, brown macroalgae represent the dominant species in intertidal and subtidal habitats. Despite conservation efforts, these canopy-forming species showed a dramatic decline, highlighting the urge for active intervention to regenerate self-sustaining populations. For this reason, the restoration of macroalgae forests through transplantation has been recognized as a promising approach. However, the potential stress caused by the handling of thalli has never been assessed. Here, we used a manipulative approach to assess the transplant-induced stress in the Mediterranean Ericaria amentacea, through the analysis of biochemical proxies, i.e., phenolic compounds, lipids, and…

Microbial Biofilms Along a Geochemical Gradient at the Shallow-Water Hydrothermal System of Vulcano Island, Mediterranean Sea

Shallow water hydrothermal vents represent highly dynamic environments where strong geochemical gradients can shape microbial communities. Recently, these systems are being widely used for investigating the effects of ocean acidification on biota as vent emissions can release high CO2 concentrations causing local pH reduction. However, other gas species, as well as trace elements and metals, are often released in association with CO2 and can potentially act as confounding factors. In this study, we evaluated the composition, diversity and inferred functional profiles of microbial biofilms in Levante Bay (Vulcano Island, Italy, Mediterranean Sea), a well-studied shallow-water hydrothermal ve…

The trophic structure of a pipefish community (Pisces: Syngnathidae) from a western Mediterranean seagrass meadow based on stable isotope analysis

Syngnathus abaster and S. typhle (Pisces. Syngnathidae) from a dense Cymodocea nodosa meadow in the western Mediterranean Stagnone di Marsala (Italy) were studied using δ13C and δ15N analysis. Because of the presence of these two species in the same habitat and the specialized parental care by the male, the effect of species and sex on the isotopic composition was also studied to investigate the different feeding strategies between and within species. S. abaster and S. typhle exhibited enriched 13C and 15N values throughout the sampling period (δ13C mean ± s.e. = -10.5 ± 1.8‰ and -10.8 ± 2.0‰, δ15N mean ± s.e. = 11.9 ± 0.7‰ and 10.6 ± 1.0‰ respectively), suggesting that these species receiv…

Short-term variability of mesozooplankton in a Mediterranean Coastal sound (Stagnone di Marsala, Western Sicily)

Short-term variations in mesozooplankton were studied during four 24-h periods at one station in a Mediterranean coastal sound (Stagnone di Marsala, western Sicily) in May, July and October 1996 and March 1997. Zooplankton samples were collected every three or six hours with a plankton net (mesh size 125 µm) towed horizontally at the surface. Hydrometric data (height of tide, speed and direction of current) were also recorded to assess the influence of the hydrodynamic conditions on fluctuations in zooplankton abundance and changes in population structure. The zooplankton assemblages were markedly dominated by copepods and their naupliar stages, which consistently accounted for more than 95…

The Effect of Temporal Changes and Environmental Trophic Condition on the Isotopic Composition (omega13C and omega15N) of Atherina boyeri (Risso, 1810) and Gobius niger (L., 1758) in a Mediterranean Coastal Lagoon (Lake of Sabaudia): Implications for Food Web Structure

δ13C and δ15N of organic matter sources and consumers were employed to analyse trophic differentiation between a benthic consumer, Gobius niger (L., 1758) (Pisces, Osteichthyes), and a pelagic consumer, Atherina boyeri (Risso, 1810) (Pisces, Osteichthyes) in a Mediterranean coastal lagoon (Lake of Sabaudia) in winter and summer 1999. Trophic differences between the two species throughout the two sampling periods were related to the environmental trophic condition (i. e. nutrient and phytopigment concentrations). Although these two fish have different habitats, they both exploited benthic organisms, above all in summer. When the nutrient and phytopigment concentrations were higher (summer), …

Effect of explosive shallow hydrothermal vents on δ13C and growth performance in the seagrass Posidonia oceanica

Summary 1. Explosive volcanic submarine activity is expected to affect seagrass communities due to sudden and dramatic changes in the physical and chemical features of sea water and sediments, with possibly large ecosystem effects. However, seagrass response to the harsh environmental conditions that arise due to explosive volcanism is as yet unexplored as it is not easy to predict when and where an eruption will occur. Here, we investigate the uptake of hydrothermal carbon within the seagrass Posidonia oceanica by the analysis of δ13C and growth rates in tissue laid down before and after an exceptional and massive hydrothermal gas release in the Aeolian Islands (Italy, Mediterranean Sea). …

Stable isotope evidence for the environmental impact of a land-based fish farm in the western Mediterranean

Isotopic examination (delta(13)C and delta(15)N) of organic matter sources and consumers was used to assess the impact and trace the dispersal of wastewater from a land-based fish farm in western Mediterranean. The results provide evidence of the non-negligible effect of aquaculture facilities on the natural stable isotopic composition of organisms. Aquaculture waste entered the food web, altering the natural isotopic composition of organic matter sources at the base and the upper trophic levels. Nitrogen-rich fish waste mainly affected delta(15)N values, while delta(13)C showed less alteration. Waste seemed to disperse widely enough to affect the isotopic composition at the study site abou…

Experimental rearing of the sea urchinParacentrotus lividusfed with discards of the lettuceLactuca sativain a sea-based system

Small-sized specimens (test diameter: 16.64 ± 0.93 mm, mean ± SE) of the sea urchin Paracentrotus lividus were fed with fresh discards of the lettuce Lactuca sativa during a 24-week experiment in a sea-based system. At the end of the experiment, stock survival was high (88.8 ± 6.6%). Sea urchin test diameter and total weight grew by 35% and 56%, respectively, while gonad somatic index, after an initial decrease, increased by 3.2%. Moreover, more than 90% of specimens achieved the gonad colour that the market demands. These results support the exploitation of L. sativa as the main ingredient in a manufactured diet for echinoculture of P. lividus. Employing diets formulated with discard ingre…

Effects of trophic and environmental conditions on the growth of Crassostrea gigas in culture

In order to study the possibility of exploiting protected marine areas, comparative data on the cultivation of the oyster Cassostrea gigas in the South Tyrrenian Sea are reported. The oysters were cultured at -7 and - 13 m on long lines linked to artificial reefs. The observations, made during a 12-month period, were of the chemical-physical and trophic properties of the water column and growth rates of the oysters. Temperature ranged between 19.81 ± 4.67°C at -7 m and 18.03 ± 3.03°C at - 13 m. Salinity showed typical Mediterranean values. The area presented oligotrophic features: the chlorophyll-a (CHLa) concentration ranged between 0.05 ± 0.01 and 0.04 ± 0.02 μg 1-1 at -7 and -13 m, respe…

Studio della connettività tra Ecosistemi Adiacenti in Ambiente Mediterraneo e Tropicale

Acquacoltura in un quadro di sviluppo sostenibile

Spatial variability of stable carbon and nitrogen isotope ratios in a Mediterranean coastal lagoon

Exploring the trophic pathway of organic matter within the Mauguio lagoon (southern France, western Mediterranean), we found spatial differences in the isotopic composition (both δ13C and δ15N values) of organic matter sources (primary producers, particulate and sedimentary organic matter), which were mirrored in the upper trophic levels (invertebrates and fish). On average, δ13C was heavier by about 1.5–2‰ in the location under marine influence than in the sites influenced by freshwater discharge. The opposite trend was found for δ15N, which attained maximum values in the north-central zone influenced by freshwater delivery. For both C and N stable isotope ratios, the highest spatial varia…

Seagrasses as biological barriers against anthropic input in coastal marine ecosystems

Posidonia oceanica loss in a Mediterranean semi-enclosed coastal system: response of polychaete assemblage

Le aree di transizione siciliane: stato dell’arte e sviluppi futuri

MOESM2 of The comparative biological effects of spatial management measures in protecting marine biodiversity: a systematic review protocol

Additional file 2. Scoping the search

Digestive Enzymes in Larvae and Juveniles of Farmed Sharpsnout Seabream (Diplodus puntazzo) (Cetti, 1777)

The ontogenetic development and pattern of main digestive enzyme activities (proteases, carbohydrases and lipases) were investigated in farmed sharpsnout seabream Diplodus puntazzo larvae and juveniles, during the passage from the larval stage to adult (from 21 to 277 days after hatching - DAH). Larvae showed exponential growth, thus confirming that Rotifers and Artemia nauplii are suitable preys for early feeding of D. puntazzo larvae. Pepsin activity was low in the larvae, but it increased afterwards in relation with stomach development. In juveniles, levels of specific activity of trypsin, chymotrypsin and elastase were lower compared to that recorded in larvae. A decrease in the activit…

Chromosomal polymorphism associated with Robertsonian fusion in Seriola dumerili (Risso, 1810) (Pisces: Carangidae)

The diploid numbers 2n= 48, and 2n= 47 have been determined for the greater amberjack, Seriola dumerili. A chromosome polymorphism due to Robertsonian fusion is present in this species. A simple sex-determining mechanism has not been observed.

The Use of Carbon Stable Isotopes to Investigate the Origin and Distribution of Suspended and Sedimentary Organic Matter in a Semi-enclosed Mediterranean Marine System

The natural stable isotope values of different primary sources have been used to trace the fate of organic carbon that enters in the marine food webs of Stagnone di Marsala (Italy). Water and sediment samples were collected monthly (March 1996 – February 1997) at 3 stations, characterized by different amounts of vegetal cover and analysed to determine total organic matter (OM), phytopigments, biopolymeric organic carbon (BPC) and stable carbon isotopic composition (δ13C), the latter measured also in main primary producers. Sedimentary OM accumulated in summer, while total suspended organic matter reached highest concentrations in May and December. The concentration of chlorophyll-a carbon i…

By-catch of cetaceans and other species of conservation concern during pair trawl fishing operations in the Adriatic Sea (Italy)

By-catch is one of the main sources of anthropogenic mortality in marine species of conservation concern worldwide. Between 2006 and 2008, the Consorzio Nazionale Interuniversitario per le Scienze del Mare (CoNISMa) coordinated a monitoring programme of cetacean by-catch in Italian pelagic trawlers, funded in compliance with European Regulation 812/2004. Sixteen independent observers monitored a total of 3141 hauls. The observation coverage ranged between 0.9 and 6.3% of the regional fishing effort. Almost all by-catch events were recorded in the northern Adriatic Sea. By-catch rates of bottlenose dolphins (Tursiops truncatus) and loggerhead turtles (Caretta caretta) were 0.0006 and 0.0255 …

Meristic character counts and incidence of skeletal anomalies in the wild Diplodus puntazzo (Cetti, 1777) of an area of the south-eastern Mediterranean Sea

The sharpsnout seabream (Diplodus puntazzo, Cetti 1777) is a species rarely found in coastal areas of Sicily, but is important in Mediterranean aquaculture. In order to detect the number of meristic characters and the main types of body deformation, 588 specimens of sharpsnout seabream were collected from a coastal zone of south-east Sicily (Licata, AG—Italy). Different sized classes were examined and the relationship between size and the number of meristic characters (number of fin rays) was found. The number of vertebrae, anal fin hard rays and dorsal fin hard rays was constant and did not vary with size. Seventeen types of skeletal and fin anomalies were observed, but no grave anomalies …

Elasmobranch by-catch in pelagic pair trawl: a preliminary study from Adriatic Sea

Fatty acid composition of a cultured sturgeon hybrid (Acipenser naccarii · A. baerii)

Analyses of fatty acids from the dorsal muscle of Acipenser naccarii · A. baerii sturgeon hybrid were carried out. The data were compared with those reported in the literature for other sturgeons reared for human consumption. This hybrid would seem to be of great nutritional interest, its flesh being more beneficial for human health than those of other cultured sturgeons. In fact, the level of polyunsaturated fatty acids (PUFAs) was very high (34.7 ± 0.67%), being similar to that of monounsaturated fatty acids (37.9 ± 0.83%). Moreover, the PUFAs-n3/PUFAs-n6 ratio (6.74) was noticeably higher in this hybrid than in the other cultured sturgeon species. There were also high contents of EPA (C2…

A new sustainable formulated feed based on discards from food industries for rearing the sea urchin Paracentrotus lividus (Lmk)

During a 18‐week experiment in a sea‐based system, Paracentrotus lividus was fed with two formulations of a new sustainable feed whose main ingredients were food farming discards, mostly outermost lettuce leaves, in different percentage. Egg white was added as protein source and binder, and a little amount of commercial fish feed was added as lipid source. At the end of the experiment, a high survival rate (&gt;80%) and an increase in test diameter (22%), total weight (130%), gonad weight (240%) and gonad index expressed as size‐adjusted gonad weight (288%) were recorded. Gonads achieved a good colour and high levels of essential and polyunsaturated fatty acids, hence representing a commerc…

Cross-validation of δ15N and FishBase estimates of fish trophic position in a Mediterranean lagoon: The importance of the isotopic baseline

Abstract FishBase, a relational database freely available on the Internet, is to date widely used as a source of quantitative information on the trophic position of marine fish species. Here, we compared FishBase estimates for an assemblage of 30 fish species sampled in a Mediterranean lagoon (Acquatina lagoon, SE Italy) with their trophic positions calculated using nitrogen stable isotopes. To assess the influence of the trophic level used to compute the baseline indicator on the robustness of isotopic estimations, we compared the trophic position of fish calculated using the average δ 15 N signature of either basal resource or primary consumer taxa measured at three stations located in th…

Sicilian transitional waters: Current status and future development

To appraise the current knowledge of Sicilian transitional waters (TWs), a review was undertaken of the information available on these ecosystems. In detail, a synthesis of the current status is reported, highlighting for each area the ecological features and status, historical data, conservation regime, environmental emergencies and anthropic pressures to which they are subject. The Sicilian TWs reviewed include coastal ponds and lakes, mires and areas with active and nonactive saltworks. Almost all of these ecosystems are affected by several protection regimes because of their high naturalistic value, although current knowledge is limited and fragmented. A few areas have received more att…

Studio della comunità associata allo strato fogliare di Posidonia oceanica in sorgenti sottomarine superficiali di CO2

Microbial communities of polluted sub-surface marine sediments

Abstract Microbial communities of coastal marine sediment play a key role in degradation of petroleum contaminants. Here the bacterial and archaeal communities of sub-surface sediments (5–10 cm) of the chronically polluted Priolo Bay (eastern coast of Sicily, Italy), contaminated mainly by n-alkanes and biodegraded/weathered oils, were characterized by cultural and molecular approaches. 16S-PCR-DGGE analysis at six stations, revealed that bacterial communities are highly divergent and display lower phylogenetic diversity than the surface sediment; sub-surface communities respond to oil supplementation in microcosms with a significant reduction in biodiversity and a shift in composition; the…

Seagrass litter decomposition in a semi-enclosed coastal ecosystem: a litterbag experiment

Rearing of sharpsnout seabream (Diplodus puntazzo, Cetti 1777) in a Mediterranean fish farm: monoculture versus polyculture

Diel Feeding Features of Juveniles of Two Sparids in the Stagnone di Marsala Coastal Sound (Western Sicily, Italy)

Diet composition, feeding rhythms, gastric evacuation rates, daily rations of Diplodus puntazzo (Gmelin 1789) and Sarpa salpa (Linneo 1758) juveniles were studied in order to investigate their trophic ecology, niche breadth and overlap. These Sparids were collected in the Stagnone di Marsala (Western Sicily), in April 1997, in a 24 hour sampling period. Gastric content of 86 specimens of D. puntazzo (standard length = 31.9 ± 6.6 mm; body wet weight = 1.0 Ī 0.5 g) and of 100 specimens of S. salpa (standard length = 32.3 ± 2.9 mm; body wet weight = 0.6 Ī 0.2 g) were examined. Amphipoda and Tanaidacea were the most frequent prey items in stomach content of D. puntozzo, while Hydrozoa, Bryozoa …

Effect of a new formulated diet based on terrestrial vegetables on roe yield and quality in the sea urchin Paracentrotus lividus (L. 1816)

Sources of carbon and dietary habits of new Lessepsian entry Brachidontes pharaonis (Bivalvia, Mytilidae) in the western Mediterranean

The sources of carbon and the dietary habits of Brachidontes pharaonis (Mollusca, Bivalvia), a new Lessepsian entry in the western Mediterranean, living in a cooling vat of a saltworks system in western Sicily (MED), were assessed by estimating throughout a season the relative abundance of a stable carbon isotope (δ13C) in particulate organic matter (POM), sedimentary organic matter (SOM), primary organic matter sources (seagrasses, sand microflora, macroalgae), Brachidontes pharaonis and its biodeposition material. In the saltworks the most enriched primary food source potentially fuelling the saltworks food web was Cymodocea nodosa (seasonal average -7.9±0.6‰), Laurencia papillosa and Cys…

The Stagnone di Marsala, western Sicily

Studio degli isotopi stabili di carbonio ed azoto e dei metalli pesanti in diverse specie di Mugilidi provenienti da aree lagunari mediterranee

Role of peat organic matter on isotopic composition of most abundant benthic organisms in intertidal habitats of SW Iceland

The transfer of peat organic matter (OM) from tidal pools of upper littoral to downstream rocky shores and its potential incorporation into marine biota were investigated using the stable isotope approach. Samplings were carried out in September 2004 in two SW Icelandic sites (Osar and Hvassahraun), where we selected (1) areas with shores where grass gently declined towards rocks and, on reaching the sea line, formed small tidal peat pools and (2) areas where grass and rocky shores were not contiguous, rather the grass lower limits were several hundreds of meters back from the shore. In both grass and no grass areas, in the intertidal zone, all benthic organisms and all potential OM sources…

Ottimizzazione delle procedure di estrazione degli IPA e valutazione della possibilità di utilizzare Sabella spallanzanii come bioaccumulatore

Diel feeding habits of juveniles of Mullus surmuletus (Linneo, 1758) in the lagoon of the Stagnone di Marsala (Western Sicily, Italy)

Diet composition, feeding rhythm, gastric evacuation rate and daily ration were investigated in juvenile Mulhis surmuletus (Linneo, 1758). Fish were collected in the lagoon of the Stagnone di Marsala in western Sicily, in July 1995, during a 24 h sampling period. Copepoda, Polychaeta, Amphipoda and Tanaidacea were shown to be the most frequent prey items. The feeding index values showed two different daily feeding times. A unimodal trend in the daily rhythm of food consumption was derived, with a peak in feeding between 1200 and 2000 h. Gastric evacuation in juvenile M. surmuletus is best described by an exponential model, with a gastric evacuation rate R = 0.66 g h-1 (r = 0.88) (T = 24.45 …

MOESM3 of The comparative biological effects of spatial management measures in protecting marine biodiversity: a systematic review protocol

Additional file 3. Data extraction

Impacts of marine aquaculture at large spatial scales: evidences from n and p catchment loading and phytoplankton biomass

International audience; While several studies point at off-shore aquaculture as a possible source of impacts on the local marine environment, very few have analysed its effects at large scales such as at the bay, gulf or basin levels. Similar analyses are hampered by the multiple sources of disturbance that may concomitantly affect a given area. The present paper addresses these issues taking the Gulf of Castellammare (Southern Tyrrhenian Sea) as an example. Nitrogen (N) and phosphorous (P) loads were calculated for the period 1970-2007, and compared to chlorophyll-a concentration as measured inside and outside the Gulf over the same period. Results indicate that N and P catchment loading h…

Study of the origin and fate of organic matter in a tropical bay using elemental, isotopic and fatty acid biomarkers.

Organic matter (OM) exchanges between adjacent coastal ecosystems affect the role of the different primary producers as energy and nutrient sources in food webs. Elemental and isotope parameters (C/N ratios, δ13C) and fatty acid (FA) biomarkers were used to assess the origin and distribution of OM in both surface sediment and water along a strongly tidally-influenced tropical area, Gazi Bay (Kenya), in two climatic seasons (dry and rainy). Dominant primary producers (i.e. mangroves, macroalgae and seagrasses) were also characterized through the same combined approach. Export of the mangrove-derived OM, highly depleted, to the adjacent bay was evident from a gradual 13C-enrichment of primary…

Influence of hydrodynamic conditions on the production and fate ofPosidonia oceanicain a semi-enclosed shallow basin (Stagnone di Marsala, Western Sicily)

An integrated approach using hydrodynamic and transport numerical models, lepidochronology and stable isotope analysis was used to investigate how local hydrodynamic conditions influence the primary production and fate of the seagrass Posidonia oceanica in a Mediterranean semi-enclosed marine system (Stagnone di Marsala). The water mass exchange aptitude of different sectors of the basin was analysed, and data collected were used to select two sectors (colonized by Posidonia oceanica showing the lowest and highest water exchange values) for biological analyses. According to the mean dispersal coefficient differences simulated by the hydrodynamic model, growth rate and primary production of …

Spatial variability of soft-bottom macrobenthic communities in northern Sicily (Western Mediterranean): Contrasting trawled vs. untrawled areas

13 páginas, 9 tablas, 5 figuras

The use of fish communities as a biological quality element to monitor ecological status of transitional waters

Polymer aging affects the bioavailability of microplastics-associated contaminants in sea urchin embryos

Microplastics (MPs) in the marine environment undergo complex weathering factors that can affect their ability to interact with different coexisting environmental contaminants (termed here co-contaminants). In this study, the influence of artificially aging using UV on the sorption of a complex mixture of co-contaminants onto MPs was investigated in order to provide meaningful hypotheses on their individual and combined toxicities on sea urchin embryos. A mixture of artificially aged MPs (PS particles and PA microfibers) combined with 2,2′ ,4,4′ - tetrabromodiphenyl ether (BDE-47), or Cd or Cu, both alone and in a mix, were used to expose embryos of Paracentrotus lividus. The effects of pol…

Influence of seabird guano on the contamination level of a Sicilian coastal area (Marinello ponds).

Notes on the Biology and Ecology of Opeatogenys gracilis (Canestrini 1864) (Pisces: Gobiesocidae) from Coastal Environments in Sicily (Mediterranean)

A particularly large number of specimens of the Mediterranean coastal fish Opeatogenys gracilis (Canestrini 1864) (Pisces: Gobiesocidae) were collected from Posidonia oceanica mats of the “Stagnone di Marsala” sound (western Sicily) and in the Gulf of Carini (northern Sicily). A study was made of biometric and meristic characters and, for the first time for this species, diet composition. The biometric and meristic data did not distinguish between specimens from the two study sites. In accordance with its benthic habit, O. gracilis feeds on small phytal prey living on Posidonia oceanica leaves. The preferential food items throughout the year were Copepoda Harpacticoida.

Signals from the deep-sea: Genetic structure, morphometric analysis, and ecological implications of Cyclothone braueri (Pisces, Gonostomatidae) early life stages in the Central Mediterranean Sea

Abstract Cyclothone braueri (Stomiiformes, Gonostomatidae) is a widely distributed fish inhabiting the mesopelagic zone of marine tropical and temperate waters. Constituting one of the largest biomasses of the ocean, C. braueri is a key element in most of the ecological processes occurring in the twilight layer. We focused on the ecological processes linked to early life stages in relation to marine pelagic environmental drivers (temperature, salinity, food availability and geostrophic currents) considering different regions of the Central Mediterranean Sea. A multivariate morphometric analysis was carried out using six parameters with the aim of discerning different larval morphotypes, whi…

Bottom-Up Control of Macrobenthic Communities in a Guanotrophic Coastal System

Soft bottom macrobenthic communities were studied seasonally in three coastal ponds (Marinello ponds, Italy) at increasing distances from a gull (Larus michahellis) colony to in- vestigate the effect of seabird-induced eutrophication (i.e. guanotrophication) on macro- benthic fauna.We hypothesized that enhanced nutrient concentration and organic load caused by guano input significantly alter the trophic and sedimentological condition of ponds, affecting benthic fauna through a bottom-up control. The influence of a set of envi- ronmental features on macrobenthic assemblages was also tested. Overall, the lowest macrobenthic abundances and functional group diversity were found in deeper sites,…

Valutazione del bioaccumulo di metalli pesanti nel polichete Sabella spallanzani (Gmelin 1791) (Sabellidae) in tre aree portuali della Sicilia

Study of benthic foraminifera in the Continental Shelf of the Gulf of Gela (Sicilian Channel) and their possible use as bioindicators

Fattori limitanti e regolatori

Sepiolinae (Mollusca: Cephalopoda) from the Strait of Sicily

Nine species belonging to three genera of the subfamily Sepiolinae (Leach, 1817) were collected in the Strait of Sicily by two series of bottom trawl surveys carried out during the years 1985-87 and 1992: Sepiola rondeletii, Sepiola intermedia, Sepiola ligulata, Sepiola robusta, Sepiola affinis, Sepietta oweniana, Sepietta neglecta, Sepietta obscura and Rondeletiola minor. For each species, abundance, distribution, sex ratio and maturity condition were analysed. S. oweniana was the most abundant species, followed by R. minor, while the single record of S. ligulata indicates that the species is rare in the area. The bathymetric distributions observed generally agree with what is known for th…

Seagrasses along the Sicilian coasts

All seagrass species known from the Mediterranean basin have been recorded along the Sicilian coast, where studies have been carried out at a very local scale and information is fragmented or confined to the grey literature. The objective of this article is to summarise and evaluate current knowledge on seagrass species on the Sicilian coasts, providing an overview of species distribution, genetic diversity, biology and ecology, based on the literature and unpublished data. Most literature studies have been carried out on Posidonia oceanica meadows because of their wide distribution, complexity and ecological importance. In this study, the analyses carried out on P. oceanica structural and …

Sources of organic matter for inter-tidal consumers in Ascophyllum-shores (Sw Iceland): a multi stable isotope approach.

Stable isotopes were used to examine the origin of organic matter in Icelandic Ascophyllum-based habitats, the role of different organic matters in filling intertidal food webs and the food preferences of the most abundant suspension feeders, grazers and predators. We selected three intertidal sites on the SW coast of Iceland where we sampled in early September 2004, organic matter sources (POM, SOM and most abundant primary producers, A. nodosum and F. vesciculosus) and the most abundant macrofauna species (barnacles, mussels, gastropods, sponge and crabs). Even though the primary production (Ascophyllum-based) was the same at the three study sites, the isotopic composition of common-among…

The importance of dead seagrass (Posidonia oceanica)matte as a biogeochemical sink

We assessed the potential of dead seagrass Posidonia oceanica matte to act as a biogeochemical sink and provide a coherent archive of environmental change in a degraded area of the Mediterranean Sea (Augusta Bay, Italy). Change in sediment properties (dry bulk density, grain size), concentration of elements (Corg, Cinorg, N, Hg) and stable isotope ratios (δ13C, δ 15N) with sediment depth were measured in dead P. oceanica matte and unvegetated (bare) sediments in the polluted area, and an adjacent P. oceanica meadow. Principal Component Analysis (PCA) revealed a clear clustering by habitat, which explained 72% of variability in our samples and was driven mainly by the accumulation of N and H…

The effect of seagrass loss on macrozoobenthos community in a Mediterranean semi-enclosed coastal system

Anomalies in Sepiolinae hectocotylization

Four cases of anomalies of the copulatory arm in Sepiolinae (Cephalopoda: Sepiolidae) are reported. In one case (Sepietta oweniana), both dorsal arms were modified resulting in double hecocotylization. In another specimen probably of the same species the copula-tory apparatus was formed by two fleshy multilobed formations situated on the dorsal and ventral side of the arm base. In one specimen of Rondeletiola minor the copulatory apparatus was inversely positioned on the arm base, and in one Sepioh intermedia specimen the inner free portion of the wrinkled tubercle was markedly enlarged.

Trophic structure of vermetid reef community: High trophic diversity at small spatial scales

Stable isotopes were used to investigate contributions of autochthonous (i.e. benthic: epilithon and macroalgae) and allochthonous (i.e. pelagic: phytoplankton) organic matter sources to the diet of suspension-feeders, grazers and predators associated to small reef-pools (cuvettes) created by the reef-building species Dendropoma petraeum in the north-western coast of Sicily (Italy). Contributions of potential food sources were calculated using Bayesian mixing-models and integrated to a multivariate approach to highlight the diversity of C and N pathways within Dendropoma cuvettes. Both pelagic and benthic organic matter sources were exploited by benthic consumers, although clear differences…

Trophic Transfer of Trace Elements in an Isotopically Constructed Food Chain From a Semi-enclosed Marine Coastal Area (Stagnone di Marsala, Sicily, Mediterranean)

Trace element accumulation is particularly important in coastal and transitional environments, which act as contaminant buffers between the continental and marine systems. We compared trace element transfer to the biota in two locations with different open-sea exposures in a semi-enclosed marine coastal area (Stagnone di Marsala, Sicily, Italy) using isotopically reconstructed food chains. Samples of sediment, macroalgae, seagrasses, invertebrates, fish, and bird feathers were sampled in July 2006 and analysed for stable carbon and nitrogen isotopes (δ(13)C, δ(15)N) and trace elements (arsenic [As], cadmium [Cd], total mercury [THg], and lead [Pb]). Trophic magnification factors were calcul…

Approccio isotopico per lo studio dell’impatto di attività di acquacoltura in ambiente marino costiero

Valutazione dei livelli di alcuni metalli pesanti in una catena del detrito ricostruita attraverso l’analisi degli isotopi stabili di carbonio ed azoto

Trace metal contamination in a Mediterranean coastal pond (Acquatina, Apulia, Italy).

The trophic structure of a fish assemblage in a non-tidal Mediterranean Lagoon: integrating data from the Fishbase information system with results from stable isotopes analysis

Shape change during the growth of sharpsnout seabream reared under different conditions in a fish farm of the southern Tyrrhenian Sea

The fate of terrestrial and riverine materials in a Mediterranean seagrass bed

Origin and distribution of suspended organic matter as inferred from carbon isotope composition in a Mediterranean semi-enclosed marine system

The origin and distribution of suspended organic matter, the trophic features and the stable carbon isotopic composition of particulate organic carbon (POC) were studied monthly in a Western Mediterranean semi-enclosed basin. Sampling stations were selected as a function of wind-exposure and the degree of vegetation cover and then compared with an adjacent unvegetated site. The predominant vegetation was seagrass (Posidonia oceanica and Cymodocea nodosa) and Caulerpa prolifera. Water samples were analyzed for total suspended matter (inorganic and organic fractions), photosynthetic pigments (chlorophyll-a and phaeopigments), dissolved organic carbon, particulate organic carbon and their isot…

Cultivation of the Mediterranean amberjack, Seriola dumerili (Risso, 1810), in submerged cages in the Western Mediterranean Sea

The growth rate, survival and food conversion ratio (FCR) of the Mediterranean amberjack (Seriola dumerili, Risso, 1810) was ascertained in cultivation using submerged net cages in the Gulf of Castellammare (NW Sicily), from September to December 1994. Two net cages (volume = 75 m3) were placed at a depth of 10 m in a sheltered area 1000 m off the coast. Juveniles (mean total length = 141.4 ± 34 2 mm; mean total wet weight = 48 ± 28.1 g) were caught in the gulf under floating wreckage with a purse seine and transplanted to the cages (n = 800 per cage) in August. Fish in one cage, group A, were fed with fish scraps whilst fish in group B were fed with pellets The total length and body wet we…

Screening of ecotoxicological, qualitative and reproductive variables in male European sea bassDicentrarchus labrax(L.) reared in three different fish farms: Facility location and typology

The aim of this work was to do a preliminary seasonal screening of ecotoxicological biomarkers in European sea bass Dicentrarchus labrax in three different fish farms, to know if the different location and typology can discriminate them. A set of selected biomarkers of xenobiotic exposure, such as acetylcholinesterase (AChE) activity, Glutathione (GSH) and gonad morphology were investigated seasonally in male European sea bass D. labrax (L.) reared in three different intensive farms: a land-based farm of cement tanks (T), an in-shore sea cages farm (C1) and an off-shore sea cages farm (C2). The results showed that both location and typology can discriminate AChE activity, GSH content and go…

Linking the effects of the Global Change to biodiversity and ecosystem services in shallow waters

Sedimentary record of anthropogenic chemical and organic pollution in a Mediterranean coastal area (Gulf of Palermo, Italy).

The relatively high influx of unregulated industrial and domestic effluents has progressively degraded the Palermo coastal environment in NW Sicily. To evaluate the poorly known effects of these inputs on the Palermo Gulf, three sediment box-cores were collected along a transect perpendicular to the coast, in front of the Oreto river mouth, in water depths of 20—750 m. The samples, dated by 210Pb and 137Cs, were analysed for concentration of major/trace elements, total polycyclic aromatic hydrocarbon (PAH), total organic carbon (TOC) and for organic carbon and nitrogen isotopic compositions. Total organic carbon concentrations show relatively high values (0.4—2.9%), with a decreasing seawar…

The origin and fate of organic matter as a tool to assess anthropogenic disturbance in coastal environments

Highly contaminated areas as sources of pollution for adjoining ecosystems: The case of Augusta Bay (Central Mediterranean).

An assessment of trace element and polycyclic aromatic hydrocarbon (PAH) contamination based on surface sediments collected in summer 2012 was carried out in Priolo Bay adjoining one of the most polluted areas of the Mediterranean Sea, the industrial Augusta harbour (Italy, Central Mediterranean). Inorganic and organic contaminants were generally not remarkable. Occasional elevated concentrations of Hg, Cd, Ni and PAHs exceeding sediment quality guidelines were detected in the northern sector of Priolo Bay, close to Augusta harbour, possibly as a result of water drainage of industrialised and urbanised areas and/or potential direct export of contaminated material from Augusta harbour, whose…

Indagine sul contributo delle fonti bentoniche e planctoniche alla rete trofica del reef a vermeti

Meristic variation and skeletal anomalies of wild and reared sharpsnout seabream juveniles (Diplodus puntazzo , Cetti 1777) off coastal Sicily, Mediterranean Sea

Use of fresh vegetable discards as sea urchin diet: effect on gonad index and quality

This study evaluated the exploitation of unprocessed agricultural discards in the form of fresh vegetable leaves as a diet for the sea urchin Paracentrotus lividus through the assessment of their effects on gonad yield and quality. A stock of wild-caught P. lividus was fed on discarded leaves from three different species (Beta vulgaris, Brassica oleracea, and Lactuca sativa) and the macroalga Ulva lactuca for 3 months under controlled conditions. At the beginning and end of the experiment, total and gonad weight were measured, while gonad and diet total carbon (C%), nitrogen (N%), δ13C, δ15N, carbohydrates, lipids, and proteins were analyzed. The results showed that agricultural discards pr…

Benthic microbial indicators of fish farm impact in a coastal area of the Tyrrhenian Sea

Abstract We studied the impact of organic loads due to the biodeposition of a fish farm in a non-impacted coastal area of the Tyrrhenian Sea (Western Mediterranean). Sediment chemistry and benthic microbial community were investigated from July 1997 to February 1998 on monthly basis at two stations: one was located under the fish farm, while the second was about 1 km away, and served as a reference site. The presence of the cage induced rapid changes in the benthic conditions: the sediments were rapidly (after 6 weeks) reduced. A significant accumulation of biopolymeric carbon was observed beneath the cage both 2 weeks after the initial cage deployment, on non-impacted sediments, and 5-7 mo…

The carrying capacity for Mediterranean bivalve suspension feeders: evidence from analysis of food availability and hydrodynamics and their integration into a local model

Abstract In order to assess the carrying capacity of two Mediterranean areas, the Incze et al. model and its modification were applied. Our measures were carried out in the Gulf of Gaeta (Central MED), where mussels ( Mytilus galloprovincialis ) are intensively cultivated (production of approximately 200 t per year) and the Gulf of Castellammare (Southern MED), where bivalve culture is not widely practised. Velocities of water current and in field filtration rates were measured in each area. Total suspended matter (TSM), suspended chlorophyll-a (CHLa), lipid, protein and carbohydrate concentrations in the particulate were measured seasonally and used as tools to evaluate the trophic status …

Meiofauna and benthic microbial biomass in a semi-enclosed mediterranean marine system (Stagnone di Marsala, Italy)

Microbial and meiofaunal dynamics and their relationships with the biochemical composition of the sedimentary organic matter were investigated in a semi-enclosed marine system (Marsala lagoon, Western Sicily, Mediterranean Sea). Sediment samples were collected on a monthly basis from March 1996 to February 1997 in four stations located along a N–S transect characterized by different hydrodynamic regimes. Total sedimentary organic matter concentration ranged from 5.681.11 to 156.2812.63 mg g1, while the biopolymeric fraction of organic carbon (BPC, measured as sum of the lipids, carbohydrates and proteins) accounted for only a small fraction (24%) of total organic matter. Total meiofaunal de…

Studio dell’impatto di un’attività di maricoltura attraverso l’analisi della Materia Organica Sedimentaria

Trace metal transfer in the food webs of a semi-enclosed coastal ecosystem (Stagnone di Marsala, Sicily, Italy)

The relationship between food availability and growth in Mytilus galloprovincialis in the open sea (southern Mediterranean)

Abstract With the aim of gathering information about the possibility of culturing mussels ( Mytilus galloprovincialis ) in a south Mediterranean oligotrophic area, different lots of mussels were placed in culture at depths of −5 m and −15 m and their growth monitored on a monthly basis. Temperature and salinity were measured in situ and water samples were collected at different depths each month. Total suspended matter (TSM) and its inorganic (ISM) and organic (OSM) fractions were analysed by gravimetry and loss on ignition. Photosynthetic pigments (chlorophyll- a and phaeopigments), particulate organic carbon (POC) and nitrogen (PON), particulate carbohydrate (CHO), protein (PRT) and lipid…

Nitrogen stable isotopes as tracers of biodeposition from a tuna farm in the Western Mediterranean

Trophic adaptability shapes isotopic niche of the resident fish Aphanius fasciatus across lagoon habitats

Abstract Coastal lagoons are characterized by high habitat heterogeneity where natural habitats coexist with artificial ones, historically set up to support human activities. Increasing anthropogenic pressure may lead to progressive degradation of the most vulnerable lagoonal habitats and the associated biological communities. One of the strictly estuarine-dependent fish species that may be affected by the degradation of lagoon habitats is the South European toothcarp Aphanius fasciatus, archetype of Mediterranean lagoon residents. Carbon and nitrogen stable isotopes were used to disentangle the influence of habitat types (natural vs artificial) and fish community (multi-trophic context) on…

Studio dell’impatto di un’attività di acquicoltura sui sedimenti della costa sud-occidentale della Sicilia attraverso l’analisi della composizione biochimica della materia organica sedimentaria

Spatial variability in stable carbon and nitrogen isotope ratios in a Mediterranean coastal lagoon (Mauguio, France).

3D-Reconstruction of a Giant Posidonia oceanica Beach Wrack (Banquette): Sizing Biomass, Carbon and Nutrient Stocks by Combining Field Data With High-Resolution UAV Photogrammetry

Beach wracks are temporary accumulations of vegetal detritus that can be found along coastlines all over the world. Although beach wracks are often perceived as a nuisance for beach users, they play a crucial ecological role in carbon and nutrient connectivity across ecosystem boundaries, especially when they reach a relevant size, as in the case of the wedge-shaped seagrass accumulations called banquette. In this study, three-dimensional mapping of a giant Posidonia oceanica banquette was carried out for the first time using high-resolution UAV photogrammetry combined with field sampling and compositional and chemical analysis. The combined approach allowed a reliable estimation of the amo…

Formulazione di un mangime altamente performante per l’acquacoltura di specie erbivore ed onnivore

Spatial and temporal changes of suspended matter in relation to wind and vegetation cover in a mediterranean shallow coastal environment

Seasonal and spatial changes in seston, (POC), particulate organic carbon, (PON) particulate organic nitrogen and chlorophyll-a concentrations were studied on a monthly basis in a Mediterranean shallow coastal area (Stagnone di Marsala, Western Sicily) in order to gather information on factors controlling particulate organic matter distribution and composition. Seston concentration and composition were connected to the main physicochemical and biological driving factors, such as temperature, salinity, dissolved oxygen, wind-speed and biomass of submerged vegetation. The Stagnone di Marsala is characterized by high temperatures with strong seasonality (range: 11-28°C), while values ranged fr…

Trace metal contamination in a mediterranean coastal pond (Acquatina, Puglia)

As the interface between land and sea, transitional areas are often negatively impacted by anthropic activities. This impact is the result of many processes, including direct input of pollutants which accumulate in both sediments and aquatic organisms. The aim of this paper is to identify trace metal (As, Cd, total Hg and Pb) contamination in the Acquatina pond (Apulia, Italy), using sediment and fish (A. boyeri, C. labrosus, D. annularis, D. dentex, D. sargus, D. vulgaris, E. encrasicholus, G. paganellus, L. ramada, M. surmuletus, S. salpa, S. solea) as descriptors. Sampling was carried out in three stations (South, Central and North) and four seasons (summer and autumn 2007, winter and sp…

Aspetti ambientali dello sviluppo della maricoltura mediterranea

Stable isotopes and trophic positions of littoral fishes from a Mediterranean Marine Protected Area

Stable isotope analyses were employed to explore feeding and foraging habitats and trophic levels of littoral fishes in a western Mediterranean Marine Protected Area (Egadi Islands, Sicily, Italy). Carbon and nitrogen stable isotope ratios were measured in primary producers, invertebrates and fishes collected in December 2001 and January 2002. Fishes of the littoral region of the Egadi Islands had isotopic signatures that fell into a wider range for δ13C (about 6‰) than for δ15N (about 3‰). Carbon isotope ratios were consistent with a food web based on mixed sources and two trophic pathways leading to different fish species. Differences in the isotopic composition between islands were highe…

Effetti ambientali della stabulazione del tonno rosso (Thunnus thynnu, L. 1758): analisi del materiale sedimentato

Sex hormone profiles in Ciona intestinalis (Ascidiacea Urochordata) and their modulation by Tributyltin (TBT)

Fatty acid composition of a cultured sturgeon hybrid (×)

Abstract Analyses of fatty acids from the dorsal muscle of Acipenser naccarii  ×  A. baerii sturgeon hybrid were carried out. The data were compared with those reported in the literature for other sturgeons reared for human consumption. This hybrid would seem to be of great nutritional interest, its flesh being more beneficial for human health than those of other cultured sturgeons. In fact, the level of polyunsaturated fatty acids (PUFAs) was very high (34.7 ± 0.67%), being similar to that of monounsaturated fatty acids (37.9 ± 0.83%). Moreover, the PUFAs- n 3/PUFAs- n 6 ratio (6.74) was noticeably higher in this hybrid than in the other cultured sturgeon species. There were also high cont…

Trace element storage capacity of sediments in deadPosidonia oceanicamat from a chronically contaminated marine ecosystem

Posidonia oceanica mat is considered a long-term bioindicator of contamination. Storage and sequestration of trace elements and organic carbon (Corg) were assessed in dead P. oceanica mat and bare sediments from a highly polluted coastal marine area (Augusta Bay, central Mediterranean). Sediment elemental composition and sources of organic matter have been altered since the 1950s. Dead P. oceanica mat displayed a greater ability to bury and store trace elements and Corg than nearby bare sediments, acting as a long-term contaminant sink over the past 120?yr. Trace elements, probably associated with the mineral fraction, were stabilized and trapped despite die-off of the overlying P. oceanica…

AQUACULTURE: ENVIRONMENTAL POTENTIALITY AND CRITICALITY

Macroalgae transplant to detect the occurrence of anthropogenic nutrients in seawater of highly tourist beaches in Mediterranean islands

In the Mediterranean region, islands are among the most important tourist destinations, being sites of considerable naturalistic, historical and cultural importance. This is highly beneficial for local economies, but may also represent a threat for the environment, especially during the peak season (i.e. summer). Indeed, the sharp demographic increase concentrated in a relatively short period may negatively affect the quality of coastal marine systems and the provision of ecosystem services, producing in turn negative feedbacks on tourism industry. Nevertheless, the assessment of the environmental impact of tourism on coastal seawater has been seldom addressed. Here, we show the results of …

A GC-MS method for hormone analysis and modulation of ovarian histology and steroidogenic genes by TBT and Bisphenol-A in Ciona intestinalis

Densità e composizione isotopica di Dendropoma petraeum (Caenogastropoda, Monterosato, 1884): confronto tra le diverse porzioni della piattaforma a vermeti

Ichthyofauna Trophic structure from three adjacent Mediterranean lagoons.

Effect of the presence of the shore crab, Carcinus maenas, on the burrowing behaviour and clearance rate of the common cockle, Cerastoderma edule

Bivalves demonstrate various morphological and behavioural adaptations to reduce the risk of being attacked by predators. This paper examines how the presence of the crab Carcinus maenas (L.), a natural predator of the cockle Cerastoderma edule (L.), affects its burrowing depth and clearance or feeding rate. Cockles were placed in experimental tanks and treated with three levels of predatory disturbance: (1) unfed crab loose inside the tank, (2) unfed crab inside a cage suspended in the water column and (3) no crab present. Cockles’ burrowing depth was measured in two sediment types: mud and sand. Cockles burrowed more deeply in treatments with no crabs. Burrowing depth in sand was signific…

MOESM1 of The comparative biological effects of spatial management measures in protecting marine biodiversity: a systematic review protocol

Additional file 1. Defining the terminology

Resilience of the seagrass Posidonia oceanica following pulse-type disturbance.

Understanding the response of species to disturbance and the ability to recover is crucial for preventing their potential collapse and ecosystem phase shifts. Explosive submarine activity, occurring in shallow volcanic vents, can be considered as a natural pulse disturbance, due to its suddenness and high intensity, potentially affecting nearby species and ecosystems. Here, we present the response of Posidonia oceanica, a long-lived seagrass, to an exceptional submarine volcanic explosion, which occurred in the Aeolian Archipelago (Italy, Mediterranean Sea) in 2002, and evaluate its resilience in terms of time required to recover after such a pulse event. The study was carried out in 2011 i…

Differential response of benthic microbes and meiofauna to fish-farm disturbance in coastal sediments

Bacterial and meiofaunal abundance and biomass and their response to the disturbance induced by fish-farm biodeposition were investigated from March to October 1997 on a monthly basis at two stations of the Gaeta Gulf (Tyrrhenian Sea, Mediterranean Sea). The biopolymeric fraction of the organic matter was characterized by high concentrations which was similar at both fish-farming-impacted and control stations. Similarly, bacteria accounted for a small fraction of the biopolymeric organic carbon (<1%), while the contribution due to auto-fluorescent cell biomass (i.e. prokaryotic and eukaryotic cells displaying auto-fluorescence) to the total biopolymeric carbon was quantitatively negligible …

Comparison of different methods for extraction of polycyclic aromatic hydrocarbons (PAHs) from Sicilian (Italy) coastal area sediments.

This paper describes a work aimed at improving the conditions of an extraction method, coupling GC-MS determination, for the analysis without cleanup phase, of polycyclic aromatic hydrocarbons (PAHs) from sediment samples. The automatic Soxhlet extraction in warm mode (using Extraction System B-811 Standard, Buchi) has demonstrated advantages for automation, reduced extraction time, and lower solvent use than for conventional Soxhlet extraction. Under these conditions, the recoveries are very good as they resulted greater than 85 % and, in most of the cases, near 100 %. The repeatability is also satisfactory (relative standard deviation less than 15 %). The detection limits are also accepta…

Vegetated Habitats Trophically Support Early Development Stages of a Marine Migrant Fish in a Coastal Lagoon

Understanding the trophic ecology of early developmental stages of commercial fish species migrating between sea and coastal lagoons is crucial to effective management of nursery habitats and resulting resources. While most information on diet shift of the gilthead seabream Sparus aurata comes from studies in experimental conditions, here we investigated the trophic niche dynamics of post-larvae, juveniles and sub-adults in natural environment, hypothesising that habitat and resources use of marine and lagoonal sites will exhibit strong relationship with ontogeny of the species. Carbon and nitrogen stable isotopes showed evidence of a clear shift in the trophic niche features, trophic posit…

Lipid and fatty acid biomarkers as proxies for environmental contamination in caged mussels Mytilus galloprovincialis

Mussels (Mytilus galloprovincialis) were transplanted from a reference site (Syracuse harbour) to an impacted site (Augusta Bay) from January to July 2013 to assess the biochemical response of caged mus- sels to high trace element and polycyclic aromatic hydrocarbon (PAH) contamination, using lipid and fatty acid (FA) biomarkers. Sediment and mussels were analysed to assess contaminant bioavailability in the study sites and bioaccumulation in mussel tissue. Trace elements (As, Cd, Co, Cr, Cu, Hg, Mn, Ni, Pb, V, Zn) and PAHs were significantly higher in mussels from Augusta than in those from Syracuse, mirror- ing the different environmental contamination. The biological impact quotient (BIQs…

Turning waste into gold: Sustainable feed made of discards from the food industries promotes gonad development and colouration in the commercial sea urchin Paracentrotus lividus (Lamarck, 1816)

Abstract Development of sustainable aquaculture practices is a suitable solution to reduce the pressure on overexploited stocks of the Mediterranean sea urchin, Paracentrotus lividus, and to respond to the increasing market demand. To move forward the Blue Growth and following the principles of circular economy, a three-month feeding experiment was conducted to test a sustainable feed based on food processing discards on sea urchins. Two feed formulations differing on the proportions of the two main ingredients (endive outermost leaves and European anchovy discards in a ratio of 60:40 and 80:20 respectively) were prepared and tested on P. lividus gonad yield, development and quality. The re…

Definizione dello stato trofico nelle Saline del trapanese attraverso lo studio della materia organica sedimentaria e particellata

Nematode community response to fish-farm impact in the western Mediterranean.

A previous investigation on fish-farm biodeposition effects on benthos, carried out in the Gaeta Gulf (northwestern Mediterranean Sea), revealed a strong impact on meiofaunal assemblages. This study implements these findings by examining in detail the nematode assemblage and its response to organic enrichment from the start of a fish farm activity to the conclusion of the fish rearing cycle. Density, community structure and individual size were utilised for univariate (genus, trophic diversity and abundance patterns) and multivariate analyses (MDS) in order to identify the best descriptors of impact and the response of the nematode assemblages. Nematodes displayed significantly reduced dens…

Chromosomal location polymorphism of major rDNA sites in two Mediterranean populations of the killifih Aphanius fasciatus (Pisces: Cyprinodontidae)

The chromosomes of the Mediterranean killifish, Aphanius fasciatus from two populations, the Lagoon of Venice (LV, 15 specimens) and the Lagoon ‘Stagnone di Marsala’ (Sicily) (SM, 48 specimens), have been investigated using conventional Ag-staining and fluorescent in situ hybridization (FISH) with 18S rDNA probe. The two methods revealed variation in the number of major rDNA sites ranging from 8 to 14 (LV) and from 1 to 4 (SM) per individual. The fact that each individual possessed its own number of sites implies that observed variation was structural. Moreover, overlapping of silver staining and FISH patterns demonstrated that all ribosomal genes were transcriptionally active in each speci…

Seasonal screening of AChE, GSH and gonad histology, in European sea bassDicentrarchus labraxL. reared in three different fish farms

The aim of this work was to do a preliminary seasonal screening of ecotoxicological biomarkers in European sea bass Dicentrarchus labrax in three different fish farms, to know if the different location and typology can discriminate them. A set of selected biomarkers of xenobiotic exposure, such as acetylcholinesterase (AChE) activity, Glutathione (GSH) and gonad morphology were investigated seasonally in male European sea bass D. labrax (L.) reared in three different intensive farms: a land-based farm of cement tanks (T), an in-shore sea cages farm (C1) and an off-shore sea cages farm (C2). The results showed that both location and typology can discriminate AChE activity, GSH content and go…

Le fanerogame marine in Sicilia

Formulation of a new sustainable feed from food industry discards for rearing the purple sea urchin Paracentrotus lividus

The lack of suitable feeds for echinoculture has led to use natural resources already widely exploited by human activities. To move towards a higher sustainability of echinoculture, this study proposes a sustainable feed for Paracentrotus lividus. Two experimental formulations were obtained using discarded endive (Cichorium endivia) leaves and anchovy (Engraulis encrasicolus) industry discards in different proportions, and agar as a binder. The evaluation of the feed stability showed that the feed was stable for 72 hr, allowing a suitable feeding for sea urchins. Both formulations showed a proper nutritional value and fatty acid profile, corresponding to the features of the main ingredients…

Initial Fish-Farm Impact on Meiofaunal Assemblages in Coastal Sediments of the Western Mediterranean

We studied the initial impact of organic loads due to the biodeposition of a new fish farm in a non-impacted coastal area of the Tyrrhenian Sea (Western Mediterranean). Sediment chemistry and meiofaunal assemblages were investigated from July 1997 to February 1998 on a monthly basis at two stations: the first was located under the fish farm, while the second was at about 1 km distance, and served as control. Variations in the biochemical composition of the sedimentary organic matter and meiofaunal community structure were also related to changes in reared fish biomass. The presence of the cage-induced rapid changes in the benthic compartment: the sediments reached rapidly (i.e., after only …

Influence of seabird guano on the trophic status and contamination levels of a Sicilian transitional area

Effects of aquaculture activities on seagrass ecosystems: evidence from stable isotopes

Environmental effects of tourism and its seasonality on Mediterranean islands: the contribution of the Interreg MED BLUEISLANDS project to build up an approach towards sustainable tourism

AbstractThe Mediterranean basin is amongst the world’s main tourist destination, and its islands are a major attraction, being sites of historical, cultural and naturalistic importance. This is beneficial for the local economies, but it may also represent a great burden on the coastal environment, where other human pressures are concentrated. The awareness of detrimental side effects of tourism together with the importance to take into account social and economic facets has led to the concept of sustainable tourism, which, however, represents a goal far from being attained. A good opportunity to promote sustainable tourism was provided by the Interreg MED BLUEISLANDS project, whose main aim…

Influence of artificial reefs on the surrounding infauna: Analysis of meiofauna

We adopted a bottom-up approach in studying the effect of two artificial reefs in contrasting environmental conditions (sandy-mud and meso-eutrophic in the Adriatic Sea versus coarse sands and oligotrophic in the Tyrrhenian Sea) on the surrounding environment by assessing changes in the meiofauna. The spatial distribution of meiofaunal assemblages was established along a transect running from within each reef to well outside its direct sphere of influence, along with information on the trophic conditions of sediments (chloropigments, proteins, carbohydrates and total organic matter). Although total densities were significantly higher in the Adriatic than in the Tyrrhenian, the meiofauna dis…

Trace element and PAH bioaccumulation in caged mussels (Mytilus galloprovincialis) from an industrial area (Augusta Bay, SE Sicily)

Guano-Derived Nutrient Subsidies Drive Food Web Structure in Coastal Ponds.

A stable isotope study was carried out seasonally in three coastal ponds (Marinello system, Italy) affected by different gull guano input to investigate the effect of nutrient subsidies on food web structure and dynamics. A marked 15N enrichment occurred in the pond receiving the highest guano input, indicating that gull-derived fertilization (guanotrophication) had a strong localised effect and flowed across trophic levels. The main food web response to guanotrophication was an overall erosion of the benthic pathway in favour of the planktonic. Subsidized primary consumers, mostly deposit feeders, switched their diet according to organic matter source availability. Secondary consumers and,…

Polychlorinated Biphenyls in Sediments from Sicilian Coastal Area (Scoglitti) using Automated Soxhlet, GC-MS, and Principal Component Analysis

A methodology for the PAHs and PCBs congener determination in sediment samples has been revised. We determined the distributions of PAHs and PCBs in the superficial sediments of the Scoglitti (Italy) coastal area to provide data for comparison with other marine systems and to hypothesize the sources. Extraction yield, for PCB, was never less than 60% in most cases, while for PAHs, utilizing perdeuterated surrogate standard (benz[a]anthracene-d12 and anthracene-d10) was never less than 72%. The total concentration of the 16 PAHs investigated, expressed as the sum of concentrations, PAHs, varied from 1–5087 μg/kg of dry matrix, while the PCBs ranged from detection limit to 36 μg/kg of dry mat…

Evidence for the cascade effect in the Marine Protected Area “Isola di Ustica”

Inter-habitat connectivity in a tropical ecosystem (Gazi Bay, Kenya) as revealed by ichthyofauna

Aquaculture impact on benthic microbes and organic matter cycling in coastal Mediterranean sediments: a synthesis

Microbial assemblages and organic matter composition as well as their response to the disturbance induced by mussel and fish-farm biodeposition were compared in several areas of the Mediterranean: La Spezia (Ligurian Sea), Gaeta (Tyrrhenian Sea), Cattolica (Adriatic Sea) and Cyprus (Levantine Sea), on both unvegetated and Posidonia bed sediments. In all systems investigated, organic matter (as biopolymeric carbon) accumulated in aquaculture impacted sediments. Among the main biochemical classes, lipids appeared to be a good tracer of aquaculture impact, especially in fish-farm sediments. Exoenzymatic activities displayed higher values in sediment beneath the cages, indicating faster organic…

Cholinesterases in Ciona intestinalis (Ascidiacea, Urochordata): preliminary investigation of their potential as tools for monitoring marine pollution

Uso trofico dell’habitat da parte dell’ittiofauna in una piccola laguna costiera mediterranea

Macrobenthic communities impoverishment in response to seabird input in a Mediterranean transitional area

Holothuria tubulosa Gmelin 1791 (Holothuroidea, Echinodermata) enhances organic matter recycling in Posidonia oceanica meadows

Abstract The role of Holothuria tubulosa in organic matter recycling of Posidonia oceanica detritus was estimated in a laboratory feeding experiment. The individual mean ingestion rate of seagrass detritus by holothurians was evaluated and carbon and nitrogen stable isotopes, carbon and nitrogen content and C:N ratio analyzed in H. tubulosa , P. oceanica detritus and surface sediment. Results showed that H. tubulosa is capable of ingesting from 30% to 100% of the detritus produced by P. oceanica meadows, depending on the holothurian density. Stable isotope analysis provided evidence for the assimilation of seagrass detritus by H. tubulosa as their signatures were compatible with 1 trophic l…

Plant and sediment properties in seagrass meadows from two Mediterranean CO2 vents: Implications for carbon storage capacity of acidified oceans

Abstract Assessing the status of important carbon sinks such as seagrass meadows is of primary importance when dealing with potential climate change mitigation strategies. This study examined plant and sediment properties in seagrass meadows (Cymodocea nodosa (Ucria) Asch.) from two high pCO2–low pH Mediterranean vent systems, located at Milos (Greece) and Vulcano (Italy) Islands, providing insights on carbon storage potential in future acidified oceans. Contrary to what has been suggested, carbon content (both inorganic and organic) and its surficial accumulation decreased at high pCO2–low pH in comparison with controls. The decrease in inorganic carbon may result from the higher solubilit…

Potential of the polychaete Sabella spallanzanii as bioindicator for PAHs

Analisi delle fonti di materia organica utilizzate da Dendropoma petraeum (Mollusca, Vermetidae) (Monterosato, 1884), in relazione a diverse condizioni idrodinamiche

Seabird influence on ecological processes in coastal marine ecosystems: An overlooked role? A critical review

Abstract Seabirds are vital, but overlooked, components of coastal marine ecosystems and may connect the marine and terrestrial environment at a global scale, significantly contributing to inter-habitat connectivity and the provision of multiple ecosystem services. Although the ecological and functional role of birds in terrestrial areas, islands in particular, has been deeply studied since the last century, the same does not hold true for coastal marine areas. Given the importance of coastal areas for seabirds worldwide and, at the same time, the high vulnerability of both, looking into the role of seabirds in influencing the ecosystem functioning in coastal areas is needed nowadays. Here,…

Gull-derived trace elements trigger small-scale contamination in a remote Mediterranean nature reserve

The role of a yellow-legged gull (Larus michahellis) small colony in conveying trace elements (As, Cd, Cr, Cu, Ni, Pb, THg, V, Zn) was assessed in a Mediterranean nature reserve (Marinello ponds) at various spatial and temporal scales. Trace element concentrations in guano were high and seasonally variable. In contrast, contamination in the ponds was not influenced by season but showed strong spatial variability among ponds, according to the different guano input. Biogenic enrichment factor B confirmed the role of gulls in the release of trace elements through guano subsidies. In addition, comparing trace element pond concentrations to the US NOAA’s SQGs, As, Cu and Ni showed contamination …

Evidence for trophic structure varibility in Mediterranean coastal lagoons

Studio delle tanatocenosi a foraminiferi bentonici nel Golfo di Gela

Rearing of sharpsnout seabream (Diplodus puntazzo, Cetti 1777) in a Mediterranean fish farm: Monoculture versus polyculture

Comparison of stable isotope composition and inorganic and organic contaminant levels in wild and farmed bluefin tuna, Thunnus thynnus, in the Mediterranean Sea

Abstract Stable isotope composition (δ13C and δ15N) and persistent pollutants, including heavy metals (Hg, Cd, Pb, As, Cu, Zn) and organochlorine compounds (PCBs, HCB and p,p′-DDE), were measured in muscle and liver tissues of wild and farmed bluefin tunas to investigate the changes occurring during the farming period and to assess the quality, in terms of contaminants, of the final product. At the end of farming, the food supplied was clearly integrated into the tuna tissues as derived from stable isotope signatures, and, contrarily to the literature findings obtained for other species, farmed tunas showed slight variations in persistent elements and chemical compounds in comparison with w…

Stress and immunomodulation indicators in gilthead seabream (Sparus aurata): preliminary data concerning the alterations due to the social behaviour.

THE EFFECT OF HEAVY METALS ON BENTHIC FORAMINIFERA IN THE GULF OF GELA (SICILIAN CHANNEL)

Benthic foraminifera are very sensitive to oceanographic parameter variations, to which they rapidly response through changes in the abundance and/or richness of their associations and in morphological variations of their shell. Many studies have used benthic foraminifera as bioindicators. We investigated recent benthic foraminiferal assemblages in the Gulf of Gela (Sicilian Channel, Mediterranean Sea), examining their relative abundance, specific richness and morphological abnormalities, to highlight a possible correlation with oceanographic features and the concentration of heavy metals in sediments. Sediment was sampled in summer 2006 using box corers in four stations at increasing dista…

Co-existing with the alien: evidence for environmental control on trophic interactions between a native (Atherina boyeri) and a non-indigenous fish species (Gambusia holbrooki) in a Mediterranean coastal ecosystem

Biological invasions are a widespread problem worldwide, as invasive non-indigenous species (NIS) may affect native populations through direct (e. g., predation) or indirect (e.g., competition) trophic interactions, leading to changes in the food web structure. The trophic relationships of the invasive eastern mosquitofish Gambusia holbrooki and the native big-scale sand smelt Atherina boyeri coexisting in three Mediterranean coastal ponds characterized by different trophic statuses (from oligotrophic to hypereutrophic) were assessed in spring through isotopic niche analysis and Bayesian mixing models. The two fish relied on the distinctive trophic pathways in the different ponds, with the …

Seagrass response to high CO2 in volcanic vents

The effect of fish farming organic waste on food availability for bivalve molluscs (Gaeta Gulf, Central Tyrrhenian, MED): stable carbon isotopic analysis

Stable carbon isotope (δ13C) analysis was used in a fish-farming impacted Mediterranean area (the Gulf of Gaeta, Central Tyrrhenian) to determine the predominant carbon sources available to bivalve molluscs cultivated around fish cages. Wether the organic matter generated by fish farming was taken up by the bivalve molluscs was also investigated. Stable carbon isotope values were measured in the particulate organic carbon (POC) of samples from potential organic matter sources such as fish-pelleted feed, mollusc faecal waste and bivalve flesh. The sources of organic matter affecting the study area water column and benthic communities appeared to be terrigenous-continental, autochthonous (phy…

Fish-farming effects on benthic community structure in coastal sediments: analysis of meiofaunal recovery

To study the impact of organic enrichment and high biodeposition caused by intensive fish farming in coastal sediments, meiofaunal samples were collected on a monthly basis between March and October 1997 at two stations of the Gaeta Gulf: One under the Farmocean cage (2000 m3), while the other (the control) was located at about 1 km from an area not impacted by fish farming. The fish farm contained about 120 000 Dicentrarchus labrax (density 18 kg m -3). The most evident changes in the benthic habitat under the cage were a large accumulation of primary organic material (phytopigment concentrations up to 44 μg g-1), changes in sedimentary organic matter composition (increased lipid levels re…

I poli dell'acquacoltura italiana: Sicilia

V.Cangialosi,I.Corsi,S.Bonacci,C.Sensini, E.Puccia,S.Focardi and A.Mazzola Influence of environmental and farm conditions on Dicentrarchus labrax L.reared in three different fish farms

Dispersal and accumulation of mariculture waste in the surrounding environment: the importance of site selection

A new lessepsian species in the western Mediterranean ( Brachidontes pharaonis Bivalvia: Mytilidae): density, resource allocation and biomass

The present study reports on population dynamics and growth performance relative to a lesser known exotic invasive species ( Brachidontes pharaonis ) inhabiting the southern Mediterranean. The study was carried out in western Sicily, where B. pharaonis is present on both the submerged and emerged surfaces of a hyperhaline saltpan. Individuals were scraped, counted and measured for shell length, total weight, somatic, gonadic and shell biomass. Brachidontes pharaonis intensively colonized all hard substrates of the saltpan with annual average densities of 375 ± 293 ind. 400 cm −2 with density peaks in autumn as a function of habitat. The occurrence of juveniles was different for mediolittora…

Contribution of planktonic and benthic food sources to the diet of the reef-forming vermetid gastropod Dendropoma petraeum in the western Mediterranean

Abstract In the Mediterranean Sea, the vermetid Dendropoma petraeum (Monterosato, 1884) forms highly biodiverse reefs that have received increasing attention in recent years although very little is known about the food habit of this species. The main goal of this study was to describe the trophic role of planktonic and benthic food sources for D. petraeum . Specimens from three morphological zones of the reef (inner edge, cuvette and outer edge) at two sites with different wave exposure along the north-western coast of Sicily (Italy; western Mediterranean) were compared for δ 13 C and δ 15 N. Isotopic determinations were also carried out on potential food sources identified in epilithon, re…

Azione dell'acido domoico sull'attività colinesterasica di Tapes philippinarum, Venus gallina, Glycimeri glycimeris (Lamellibranchia, Mollusca) e Ciona intestinalis (Ascidiacea, Urochordata)

Indicatori di stress ed immunomodulazione in Sparus aurata: dati preliminari sulle modificazioni indotte dal comportamento sociale di sottomissione.

Intrinsic bioremediation potential of a chronically polluted marine coastal area.

A microbiological survey of the Priolo Bay (eastern coast of Sicily, Ionian Sea), a chronically polluted marine coastal area, was carried out in order to discern its intrinsic bioremediation potential. Microbiological analysis, 16S rDNA-based DGGE fingerprinting and PLFAs analysis were performed on seawater and sediment samples from six stations on two transects. Higher diversity and variability among stations was detected by DGGE in sediment than in water samples although seawater revealed higher diversity of culturable hydrocarbon-degrading bacteria. The most polluted sediment hosted higher total bacterial diversity and higher abundance and diversity of culturable HC degraders. Alkane- an…

Effetti ambientali della stabulazione del tonno rosso (Thunnus thynnus L.): analisi della materia organica sedimentaria

Integrating multiple stressors in aquaculture to build the blue growth in a changing sea

Fisheries currently represent the main source of animal protein intake worldwide, although catches of most commercial species are at or beyond maximum sustainable yields. Increasing production would require an excess of exploitation levels and aquaculture is expected to become crucial in sustaining a growing seafood demand. Nonetheless, many threats are expected to affect aquaculture and the increased production must evolve in a way that minimizes environmental and socio-economic impacts. The claimed sustainable development of human activities at sea (blue growth and economy) seeks for new joint analyses and solutions at (trans-)national systemic level should be planned and applied. To meet…

Trace element bias in the use of CO2 vents as analogues for low pH environments: Implications for contamination levels in acidified oceans

Abstract Research into the effects of ocean acidification on marine ecosystems has increasingly focused on natural CO 2 vents, although their intrinsic environmental complexity means observations from these areas may not relate exclusively to pH gradients. In order to assess trace element levels and distribution in the Levante Bay (Vulcano Island, NE Sicily, Italy) and its suitability for studying biological effects of pH decline, Ba, Fe and trace elements (As, Cd, Co, Cr, Cu, Hg, Mn, Mo, Ni, Pb, V and Zn) in sediment were analysed from 7 transects. Where present, Cymodocea nodosa leaves and epiphytes were also analysed. At the spatial scale of the bay, trace element concentrations in sedim…

Heavy metals and stable isotope ratios in organisms from three adjacent Mediterranean coastal lagoons

Le banquette di Posidonia oceanica in Sicilia occidentale: mappatura, tipizzazione e indagini sulla mesofauna associata.

Stable carbon and nitrogen ratios (δ13Corg and δ15Ntot) of core sediments from the Gulf of Palermo (NW Sicily): implications for the carbon source

Screening of Ovarian steroidogenic pathway in Ciona intestinalis and its modulation after Tributyltin exposure

In this study, we have identified several ovarian steroids in Ciona with high similarity to vertebrate steroids and showed that cholesterol, corticosterone, dehydroepiandrosterone, estrone, estradiol-17beta, testosterone, pregnenolone, progesterone, have identical molecular spectra with vertebrate steroids. In addition, we have studied the effects of an endocrine disruptor (tributyltin: TBT) on these sex hormones and their precursors, ovarian morphology, and gene expression of some key enzymes in steroidogenic pathway in the ovary of Ciona. Ovarian specimens were cultured in vitro using different concentrations of TBT (10{sup -5}, 10{sup -4} and 10{sup -3} M). Ethanol was used as solvent co…

Evidence for endocrine disruption of tributyltin chloride in ovary of Ciona intestinalis (Ascidiacea, Urochordata)

Comparison of growth performance and biometric relationships in two reciprocal sturgeon hybrids reared in net cages (Sicily, Mediterranean)

This study was designed to investigate growth performance in two sturgeon hybrids reared in two quadrangular floating cages moored in an artificial pond, representing the first experience of sturgeon culture performed at Southern Mediterranean latitudes. The research was carried out from December 2000 to July 2001 and tested the growth performance, feeding parameters and biometric relationships. The sturgeons were randomly collected from the cages on a monthly basis, and total length, standard length (SL) and wet weight (WW) were recorded. The specific daily growth rate (SGRW) as somatic WW, food conversion ratio and condition factor (CF) were calculated. Biometric relationships, as linear …

Studio di un modello per l ’utilizzo delle vasche fredde delle saline di Trapani ai fini di acquicoltura estensiva

Regime shifts and alternative stable states in intertidal rocky habitats: State of the art and new trends of research

Abstract The existence of regime shifts and alternative stable states in ecosystems is well known and has very large effects on their structure and dynamics. Since shifts between alternative stable states have significant implications for the ecosystems conservation, their prevention should be an aim of primary interest, and for this reason a particular attention has been paid to their study. Regarding marine ecosystems, rocky intertidal habitats, in particular, represent an ideal system for the study of alternative stable states because of their characteristics: they exhibit strong environmental gradients, are easy to manipulate, and most of the inhabiting species grow rapidly. Given the s…

Valutazione dei livelli di esposizione a contaminanti di origine antropica mediante l ’uso di biomarkers negli esemplari di Thunnus Thynnus allevati e selvatici

Polycyclic aromatic hydrocarbons (PAHs) in the polychaete Sabella spallanzanii, as a reliable bioindicator, by GCMS

Every year 230.000 tons of PAHs enter the marine environment, resulting ubiquitously distributed worldwide. These compounds are aromatic molecules of special concern because of their toxic and carcinogenic properties. As a consequence of their hydrophobic nature, in aquatic environments, PAHs rapidly tend to associate with particulate matter and sediments represent the most important reservoir. In the present study the level of PAHs in the sessile filter-feeder polychaete Sabella spallanzanii was assessed. In particular, the concentration of PAHs was determined in polychaetes (body and tube) collected from three Sicilian areas, subject to different anthropic pressure. A transplanting experi…

Environmental Constraints on Pathways of Organic Detritus in a Semi-enclosed Marine System (W-Mediterranean)

In order to assess seasonal and spatial changes in water-sediment interaction processes in a semi-enclosed marine system of Western Sicily (Marsala lagoon; W-Mediterranean), the biochemical composition of suspended and sediment organic matter was studied, during a one-year sampling period. The observed dynamic balance of resuspension vs. sedimentation processes and the macroalgal and vascular plant coverage appear major factors in affecting both amounts and biochemical composition of suspended and sedimentary organic matter and allowed us to identify two different sub-systems. The northern area, characterised by frequent wind-induced sediment resuspension events and by a scant vegetation, d…

The trophic role of the sand smelt, Atherina boyeri Risso 1810 (Osteichthyes, Atherinidae), in Mediterranean coastal environments

The fate of organic matter sources in coastal environments: a comparison of three Mediterranean lagoons

Trophic processes in coastal lagoons are strongly influenced by freshwater inputs and water exchanges with the sea. In recent years, stable isotope analysis has become a widespread and reliable method for the examination of trophic structure over time and space, also in complex ecosystems such as coastal lagoons. Stable isotopes of carbon and nitrogen were studied in primary producers and consumers to identify organic matter source pools from terrestrial, benthic and pelagic environments and to characterise the trophic structure in three Mediterranean coastal lagoons (Lake Fusaro, the Lake of Sabaudia and Stagnone di Marsala). The results highlighted the negligible importance of terrestrial…

Stagnone di Marsala Lagoon, western Sicily.

Seasonal and spatial changes in the sediment organic matter of a semi-enclosed marine system (W-Mediterranean Sea)

The composition of sediment organic matter and the related role of microphytobenthic biomass have been investigated during one-year in a semi-enclosed marine system (Marsala lagoon, Mediterranean Sea). Monthly samples from June 1993 to May 1994 were analysed for carbohydrate, protein, lipid, photosynthetic pigment and total organic matter. The three main biochemical classes of organic compounds (i.e. carbohydrates, proteins and lipids) showed higher concentrations than reported in the literature. However, photosynthetic pigment was quite low, compared to other enclosed marine basins or Mediterranean coastal lagoons. As a result, the contribution of primary organic carbon to the sedimentary …

Trophic structure and functioning of vermetid reef community based on carbon and nitrogen stable isotope analysis.

δ15N in deployed macroalgae as a tool to monitor nutrient input driven by tourism activities in Mediterranean islands

Abstract Mediterranean Sea is among the world's leading tourist destinations; however, the sharp increase in tourists during the high season may affect coastal seawater. The main aim of this study was to evaluate the occurrence and temporal variation of anthropogenic nutrients in coastal seawater in relation to tourist flows in three Mediterranean islands (Cyprus, Sicily and Rhodes), through short-term macroalgae deployments, coupled with δ15N analysis and GIS mapping. In all islands, an overall increase in macroalgae δ15N occurred over the deployment carried out in August in the tourist sites, suggesting the presence of anthropogenic nutrients. Decreasing δ15N values occurred at increasing…

Origin of carbon and nitrogen in the diet of suspension-feeding bivalve Cerastoderma glaucum (L.) in a shallow habitat (southern Mediterranean): a stable isotope study

Assessing the environmental impact of aquaculture: a new approach based on stable isotope analysis

Karyotype analysis, nucleolar organizer regions(NORs), and C-banding pattern of Dicentrarchus iabrax(L.) and Dicentrarchus punctatus(Block, 1792)(Pisces, Perciformes) with evidence of chromosomal structural polymorphism.

Chromosomes of Dicentrarchus labrax and Dicentrarchus punctatus collected from the Gulf of Palermo are examined from kidney cells. The diploid chromosome number in both species is 2n=48. Ag-NOR and C-banding analyses revealed the presence of structural chromosomal polymorphism involving pair 1.A comparative analysis among the Giemsa, NOR- and C-banded karyotypes suggests that a close phylogenetic relationship between D. labrax and D. punctatus occurs. There are evidences for a conservative evolutionary trend in this genus.

Use of stable isotopes (delta13C and delta15N) for detecting the dispersione effect of fish farming waste: a stydu case from southern Tyrrhenian Sea (MED)

Heterotrophic bacteria community and pollution indicators of mussel--farm impact in the Gulf of Gaeta (Tyrrhenian Sea).

Field studies were carried out to determine and compare the impact of organic loads due to the biodeposition of a mussel farm on the water quality and sediment in a coastal area of the Tyrrhenian Sea (Western Mediterranean). A total of five environmental and five microbial parameters were examined from March, 1997 to February, 1998 on a monthly basis at three stations: the first was located under the mussel farm, the second located at about 40 m away from the mussel farm, while the third designed as a control was at about 1-km. No clear changes in the physical characteristics of the water masses were observed, comparing the three sampling sites and the water column generally showed homogene…

A new formulated diet for sea urchin (Paracentrotus lividus) aquaculture

Analysis of the relationship between chemistry composition and stable isotope signals in coastal sediments: a biogeochemistry study to assess the environmental impact

Relazioni trofiche dell’epifauna colonizzatrice del detrito fogliare di Posidonia oceanica

Preliminary studies on reproduction of Puntazzo puntazzo (Gmelin 1789) (Pisces, Sparidae) under controlled conditions

Abstract Experiments were carried out under controlled conditions in September 1983 on the reproduction of Puntazzo puntazzo (sheepshead bream). The techniques adopted to stimulate spawning, the egg characteristics, and the phases of embryonal development until the sixth day after hatching are described. The best spawning results were obtained with intramuscular injections of HCG at 1 I.U./g of body weight. Optimal fertilization and hatching were observed at 22°C, the mean temperature of the sea water during the natural period of reproduction of Puntazzo puntazzo .

Measuring the effects of temperature rise on Mediterranean shellfish aquaculture

Abstract Shellfish aquaculture represents a worldwide valuable segment of the aquaculture market, spreading along the Mediterranean coasts, and is sensitive to the still unforeseen, poorly-known effects of climate change. Threats due to temperature rise can threaten the deployment and development of this sector, up until now recognised as the best candidate to mitigate the effects of fishery overexploitation. Here, we investigate the effects of temperature increase on the model species, Mytilus galloprovincialis, measuring outcomes from valve fragility (thickness) and condition index. Evidence of a reduction in the thickness of valves and the modulation condition of the mussels along with t…

Seasonal variations in the stable carbon and nitrogen isotope ratios (13C/12C and 15N/14N) of primary producers and consumers in a western Mediterranean coastal lagoon

Stable carbon and nitrogen isotope ratios (13C/12C and 15N/14N) of primary producers and consumers were investigated seasonally throughout 1999, in order to describe the food web in a western Mediterranean coastal lagoon (Lake of Sabaudia, central Italy). Particulate organic matter and algal material (seagrass epiphytes and macroalgae) seem to constitute the main food sources for primary consumers (zooplankton and small benthic invertebrates, respectively) throughout the sampling year, while the seagrass Cymodocea nodosa appears to play a negligible trophic role. As regards the ichthyofauna, carbon stable isotopes differentiated between planktivore and benthivore fish species. However, a be…

Trophic transfer of trace metals in an isotopically constructed food web from a Mediterranean coastal area

Registration of past submarine massive outgassing events in Posidonia oceanica L. (Delile) through stable carbon isotopes