ChemInform Abstract: Synthesis of Glycopeptides Containing Carbohydrate and Peptide Recognition Motifs

2001

Additional file 2: of Protective intraoperative ventilation with higher versus lower levels of positive end-expiratory pressure in obese patients (PR…

2017

Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) 2013 checklist: recommended items to address in a clinical trial protocol and related documents. (PDF 122 kb).

Additional file 1: of Protective intraoperative ventilation with higher versus lower levels of positive end-expiratory pressure in obese patients (PR…

2017

PROBESE Study protocol version 2.5. This PDF file includes the most recent version of the PROBESE Study protocol with changes highlighted. (PDF 870Â kb)

ChemInform Abstract: Oligosaccharide Synthesis via Electrophile-Induced Activation of Glycosyl-N-allylcarbamates.

2010

Abstract Glycosyl-N-allyl carbamates, obtained by reaction of anomerically unprotected saccharides with allyl isocyanate, can be activated by an electrophile-induced cyclisation and reacted with glycosyl acceptors to form the corresponding oligosaccharides By this method the mucin core 2 trisaccharide2 has successfully been synthesized. Due to the mild glycosylation conditions even 1-O-acetyl protected glycosyl acceptors can be used. This was demonstrated in the synthesis of a 1,6-linked glucosyl trisaccharide whereby a reptitious glycosylation strategy could be applied. 1. Dedicated to the memory of Professor Akira Hasegawa.

ChemInform Abstract: Synthetic O-Glycopeptides as Model Substrates for Glycosyltransferases.

2010

Abstract A new approach to O-glycopeptides of the glucosamine type is described. N-Urethane protected, peracetylated glucosamine is converted into its 1-thio (1-bromo) derivative and used for glycosylation of a variety of protected serine or threonine derivatives as acceptors. The urethane group is easily exchanged for the natural N-acetyl moiety and O-deacetylation is achieved with hydrazine/methanol. The resulting O-GlcNAc derivatives are subjected to an enzymatic galactosylation procedure using β-1,4-galactosyltransferase (EC 2.4.1.22) to furnish O-glycopeptides of the neolactosamine type.

Additional file 1: of Protective ventilation with high versus low positive end-expiratory pressure during one-lung ventilation for thoracic surgery (…

2019

PROTHOR-Patient information. (DOCX 19 kb)

Chemoselective Removal of Protecting Groups from O-Glycosyl Amino Acid and Peptide (Methoxyethoxy)ethyl Esters Using Lipases and Papain

1996

The selective C-terminal deprotection of O-glycopeptide (methoxyethoxy)ethyl esters is achieved under mild conditions (pH 6.6, 37 degrees C) by enzymatic hydrolysis using papain or lipase M from Mucor javanicus to give building blocks useful for chain-extending glycopeptide synthesis. On the other hand, the selective removal of acetyl protecting groups from the saccharide portion of glycopeptides is accomplished by alternative enzymatic hydrolysis with lipase WG from wheat germ to furnish model substrates for enzymatic glycosyl transfer reactions in order to extend the carbohydrate side chain of these conjugates.

ChemInform Abstract: Chemical and Enzymic Synthesis of Glycopeptides

2010

Additional file 4: of Protective intraoperative ventilation with higher versus lower levels of positive end-expiratory pressure in obese patients (PR…

2017

Standard operating procedures (SOP) for plasma Sampling. (PDF 115Â kb)

Enzymatic glycosylation of o-glycopeptides

1992

Abstract O-Glycosylation of serine derivatives carried out with N-urethane protected glucosamine yields O-glycopeptides which are regio- and stereoselectively galactosylated with the aid of β-1,4-galactosyltransferase (EC 2.4.1.22).

Additional file 1: of Protective intraoperative ventilation with higher versus lower levels of positive end-expiratory pressure in obese patients (PR…

2017

PROBESE Study protocol version 2.5. This PDF file includes the most recent version of the PROBESE Study protocol with changes highlighted. (PDF 870Â kb)

Synthetic O-glycopeptides as model substrates for glycosyltransferases

1993

Abstract A new approach to O-glycopeptides of the glucosamine type is described. N-Urethane protected, peracetylated glucosamine is converted into its 1-thio (1-bromo) derivative and used for glycosylation of a variety of protected serine or threonine derivatives as acceptors. The urethane group is easily exchanged for the natural N-acetyl moiety and O-deacetylation is achieved with hydrazine/methanol. The resulting O-GlcNAc derivatives are subjected to an enzymatic galactosylation procedure using β-1,4-galactosyltransferase (EC 2.4.1.22) to furnish O-glycopeptides of the neolactosamine type.

Additional file 3: of Protective intraoperative ventilation with higher versus lower levels of positive end-expiratory pressure in obese patients (PR…

2017

PROBESE case report form version 1.2.2. This file corresponds to the paper version of the case report form. (DOC 1610 kb).

Synthesis of Glycopeptides Containing Carbohydrate and Peptide Recognition Motifs

2000

ChemInform Abstract: Recent Advances in the Synthesis of Glycopeptides

2010

Additional file 2: of Protective intraoperative ventilation with higher versus lower levels of positive end-expiratory pressure in obese patients (PR…

2017

Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) 2013 checklist: recommended items to address in a clinical trial protocol and related documents. (PDF 122 kb).

Additional file 5: of Protective intraoperative ventilation with higher versus lower levels of positive end-expiratory pressure in obese patients (PR…

2017

Standard operating procedures (SOP) for plasma Sampling. (PDF 110Â kb)

Additional file 3: of Protective intraoperative ventilation with higher versus lower levels of positive end-expiratory pressure in obese patients (PR…

2017

PROBESE case report form version 1.2.2. This file corresponds to the paper version of the case report form. (DOC 1610 kb).

ChemInform Abstract: Glycopeptides as Signal Molecules. A Synthetic Challenge on Its Way Towards Interdisciplinary Application

2010

Stereospecific CC-bond formation with rabbit muscle aldolase - A chemoenzymatic synthesis of (+)-exo-brevicomin

1990

Abstract (+)-(1S,5R,7S)-Exo-brevicomin 9, a sex pheromone of the western pine bark beetle, is synthesized using an aldol reaction catalyzed by fructose-1,6-diphosphate aldolase (EC 4.1.2.13) from rabbit muscle as the key step by which the absolute configuration of the target is established.

Chemoenzymatische „Chiral-Pool”-Synthese von (+)-exo-Brevicomin aus Kohlenhydraten mit Fructose-1,6-diphosphat-Aldolase

1990

Chemoenzymatic “Chiral-Pool” Synthesis of (+)-exo-Brevicomin from Carbohydrates Using Fructose 1,6-Diphosphate Aldolase Fructose-1,6-diphosphate aldolase (EC 4.1.2.13) catalyzes the stereospecific aldol reaction between 1,3-dihydroxyacetone phosphate (4) and 5-oxohexanal (3) or its 5-dithiane-protected analog 8. The products of the aldol reactions are dephosphorylated with acid phosphatase (EC 3.1.3.2). Whereas the aldol adduct of 3 cyclizes spontaneously to give the bicyclic brevicomin precursor 3, the adduct of 8 first has to be deprotected with sulfuryl chloride and wet silica gel. The resulting bicyclic α-hydroxy ketone 3 is reduced with LiAlH4 to form the 1,2-diol 14 which is then deox…

Chemical and enzymatic synthesis of glycopeptides

1995

Progress recently made in the synthesis of biologically relevant N- and O-glycopeptides is illustrated by examples. In this context, developments in the preparation of complex saccharide side chains and in the subsequent coupling to peptide portions is described. Special emphasis is given to the synthesis of Lewis antigen-type structures. Furthermore, modern methods in solid phase peptide syntheses utilizing glycosylated building blocks are presented. Recent advances in glycopeptide syntheses employing enzymatic methods in deprotection steps as well as in peptide/saccharide chain elongation are reported.

ChemInform Abstract: Chemoselective Removal of Protecting Groups from O-Glycosyl Amino Acid and Peptide (Methoxyethoxy)ethyl Esters Using Lipases and…

2010

The selective C-terminal deprotection of O-glycopeptide (methoxyethoxy)ethyl esters is achieved under mild conditions (pH 6.6, 37 degrees C) by enzymatic hydrolysis using papain or lipase M from Mucor javanicus to give building blocks useful for chain-extending glycopeptide synthesis. On the other hand, the selective removal of acetyl protecting groups from the saccharide portion of glycopeptides is accomplished by alternative enzymatic hydrolysis with lipase WG from wheat germ to furnish model substrates for enzymatic glycosyl transfer reactions in order to extend the carbohydrate side chain of these conjugates.

The Association of Intraoperative driving pressure with postoperative pulmonary complications in open versus closed abdominal surgery patients – a po…

2021

Abstract Background It is uncertain whether the association of the intraoperative driving pressure (ΔP) with postoperative pulmonary complications (PPCs) depends on the surgical approach during abdominal surgery. Our primary objective was to determine and compare the association of time–weighted average ΔP (ΔPTW) with PPCs. We also tested the association of ΔPTW with intraoperative adverse events. Methods Posthoc retrospective propensity score–weighted cohort analysis of patients undergoing open or closed abdominal surgery in the ‘Local ASsessment of Ventilatory management during General Anaesthesia for Surgery’ (LAS VEGAS) study, that included patients in 146 hospitals across 29 countries.…