0000000001305921
AUTHOR
Johanna M. Alaranta
Optimizing the SYBR green related cyanine dye structure to aim for brighter nucleic acid visualization
In recent years, the studies of RNA and its use for the development of RNA based vaccines have increased drastically. Although cyanine dyes are commonly used probes for studying nucleic acids, in a wide range of applications, there is still a growing need for better and brighter dyes. To meet this demand, we have systematically studied the structure of SYBR green-related cyanine dyes to gain a deeper understanding of their interactions with biomolecules especially how they interact with nucleic acids and the structural components which makes them strongly fluorescent. Herein, five new dyes were synthesized, and their photophysical properties were evaluated. Observations of photophysical cha…
Systematic study of SYBR green chromophore reveals major improvement with one heteroatom difference
Five nucleic acid binding cyanine dyes were synthesized and their photophysical properties were evaluated. Changing a single heteroatom in the chromophore causes major differences both in brightness and photostability between the dyes. With such alteration, the brightness of the chromophore increased two-fold compared to the one found in SYBR Green I.
Systematic study of SYBR green chromophore reveals major improvement with one heteroatom difference
Five nucleic acid binding cyanine dyes were synthesized and their photophysical properties were evaluated. Changing a single heteroatom in the chromophore causes major differences both in brightness and photostability between the dyes. With such alteration, the brightness of the chromophore increased two-fold compared to the one found in SYBR Green I. peerReviewed
CCDC 2194827: Experimental Crystal Structure Determination
Related Article: Johanna M. Alaranta, Khai-Nghi Truong, María Francisca Matus, Sami A. Malola, Kari T. Rissanen, Sailee S. Shroff, Varpu S. Marjomäki, Hannu J. Häkkinen, Tanja M. Lahtinen|2022|Dyes Pigm.|208|110844|doi:10.1016/j.dyepig.2022.110844
CCDC 2194825: Experimental Crystal Structure Determination
Related Article: Johanna M. Alaranta, Khai-Nghi Truong, María Francisca Matus, Sami A. Malola, Kari T. Rissanen, Sailee S. Shroff, Varpu S. Marjomäki, Hannu J. Häkkinen, Tanja M. Lahtinen|2022|Dyes Pigm.|208|110844|doi:10.1016/j.dyepig.2022.110844
CCDC 2194823: Experimental Crystal Structure Determination
Related Article: Johanna M. Alaranta, Khai-Nghi Truong, María Francisca Matus, Sami A. Malola, Kari T. Rissanen, Sailee S. Shroff, Varpu S. Marjomäki, Hannu J. Häkkinen, Tanja M. Lahtinen|2022|Dyes Pigm.|208|110844|doi:10.1016/j.dyepig.2022.110844
CCDC 2194826: Experimental Crystal Structure Determination
Related Article: Johanna M. Alaranta, Khai-Nghi Truong, María Francisca Matus, Sami A. Malola, Kari T. Rissanen, Sailee S. Shroff, Varpu S. Marjomäki, Hannu J. Häkkinen, Tanja M. Lahtinen|2022|Dyes Pigm.|208|110844|doi:10.1016/j.dyepig.2022.110844
CCDC 2194824: Experimental Crystal Structure Determination
Related Article: Johanna M. Alaranta, Khai-Nghi Truong, María Francisca Matus, Sami A. Malola, Kari T. Rissanen, Sailee S. Shroff, Varpu S. Marjomäki, Hannu J. Häkkinen, Tanja M. Lahtinen|2022|Dyes Pigm.|208|110844|doi:10.1016/j.dyepig.2022.110844