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RESEARCH PRODUCT
Ubiquitous Structural Signaling in Bacterial Phytochromes
Matthijs R. PanmanRoberto AppioAngela C. NugentRachael St. PeterHardik PatelHeikki TakalaHeikki TakalaAlexander BjörlingKevin D. GallagherIvan RajkovicMaria HoernkeEmina A. StojkovićHeli LehtivuoriTilman LamparterPhu DuongRajiv HarimoorthyEmil GustavssonFan ZhangPeter BerntsenPeter BerntsenSebastian WestenhoffJanne A. IhalainenOskar BerntssonStephan Nieblingsubject
0303 health sciencesBacteriaPhytochromeProtein dynamicsta1182BiologyX-ray scattering010402 general chemistryBioinformaticsphytochromes01 natural sciences0104 chemical sciences/dk/atira/pure/sustainabledevelopmentgoals/clean_water_and_sanitation03 medical and health sciencesprotein dynamicsBiophysicsGeneral Materials SciencePhytochromePhysical and Theoretical ChemistrySignal transductionSDG 6 - Clean Water and SanitationHistidinesignal transduction030304 developmental biologydescription
The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-09-03 | Journal of Physical Chemistry Letters |