6533b7d2fe1ef96bd125f6ec

RESEARCH PRODUCT

Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma

Silvia Adriana López De BlancRosana Andrea MorelattoDaniel PicciniNatalia GuiñazúMaría-elina ItoizSusana Gea

subject

MalePathologyNitric Oxide Synthase Type IImedicine.disease_causechemistry.chemical_compoundField cancerizationMedicineNitriteMouth neoplasmAged 80 and overbiologymedicine.diagnostic_testCarcinoma oral de células escamosasNitric oxide synthase 2Patología//purl.org/becyt/ford/3.1 [https]Middle Aged:CIENCIAS MÉDICAS [UNESCO]Ciencias de la saludMedicina BásicaOral squamous cell carcinomaUNESCO::CIENCIAS MÉDICASCarcinoma Squamous CellImmunohistochemistryFemaleMouth Neoplasms//purl.org/becyt/ford/3 [https]Adultmedicine.medical_specialtyCIENCIAS MÉDICAS Y DE LA SALUDOdontologíaBiopsyparasitic diseasesCarcinomaHumansNitric Oxide Synthase 2 (NOS2)Cancerización de campoGeneral DentistryAgedMarcadores de malignidadOral Medicine and Pathologybusiness.industryResearchmedicine.diseasestomatognathic diseasesCross-Sectional StudiesOtorhinolaryngologychemistrybiology.proteinSurgeryField cancerizationbusinessCarcinogenesisMalignity markers

description

The activity of Nitric Oxide Synthase 2 (NOS2) was found in oral squamous cell carcinomas (OSCC) but not in normal mucosa. Molecular changes associated to early carcinogenesis have been found in mucosa near carcinomas, which is considered a model to study field cancerization. The aim of the present study is to analyze NOS2 expression at the histologically normal margins of OSCC. Study Design: Eleven biopsy specimens of OSCC containing histologically normal margins (HNM) were analyzed. Ten biopsies of normal oral mucosa were used as controls. The activity of NOS2 was determined by immunohistochemistry. Salivary nitrate and nitrite as well as tobacco and alcohol consumption were also analyzed. The Chi-squared test was applied. Results: Six out of the eleven HNM from carcinoma samples showed positive NOS2 activity whereas all the control group samples yielded negative (p=0.005). No statistically significant association between enzyme expression and tobacco and/or alcohol consumption and salivary nitrate and nitrite was found. Conclusion: NOS2 expression would be an additional evidence of alterations that may occur in a state of field cancerization before the appearance of potentially malignant morphological changes. Fil: Morelatto, Rosana Andrea. Universidad Nacional de Córdoba; Argentina Fil: Itoiz, María Elina. Universidad de Buenos Aires; Argentina Fil: Guiñazu Alaniz, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas; Argentina Fil: Piccini, Daniel. Universidad Nacional de Córdoba; Argentina Fil: Gea, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Lopez de Blanc, Silvia Adriana. Universidad Nacional de Córdoba; Argentina

yearjournalcountryeditionlanguage
2014-05-07