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RESEARCH PRODUCT
Whi7 is an unstable cell-cycle repressor of the Start transcriptional program
Ester MéndezMercè Gomar-albaM. Carmen BañóJ. Carlos IgualInma Quilissubject
0301 basic medicineSaccharomyces cerevisiae ProteinsTranscription GeneticCell divisionScienceGeneral Physics and AstronomyRepressorSaccharomyces cerevisiaeBiologyArticleGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesCyclinsGene Expression Regulation Fungallcsh:ScienceGeneticsRegulation of gene expressionCyclin-dependent kinase 1MultidisciplinaryYY1QPromoterCell Cycle CheckpointsGeneral ChemistryCell cycleRepressor Proteins030104 developmental biologyGATAD2Blcsh:Qdescription
Start is the main decision point in eukaryotic cell cycle in which cells commit to a new round of cell division. It involves the irreversible activation of a transcriptional program by G1 CDK-cyclin complexes through the inactivation of Start transcriptional repressors, Whi5 in yeast or Rb in mammals. Here we provide novel keys of how Whi7, a protein related at sequence level to Whi5, represses Start. Whi7 is an unstable protein, degraded by the SCFGrr1 ubiquitin-ligase, whose stability is cell cycle regulated by CDK1 phosphorylation. Importantly, Whi7 associates to G1/S gene promoters in late G1 acting as a repressor of SBF-dependent transcription. Our results demonstrate that Whi7 is a genuine paralog of Whi5. In fact, both proteins collaborate in Start repression bringing to light that yeast cells, as occurs in mammalian cells, rely on the combined action of multiple transcriptional repressors to block Start transition.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-08-01 | Nature Communications |