NAFLD and Atherosclerosis Are Prevented by a Natural Dietary Supplement Containing Curcumin, Silymarin, Guggul, Chlorogenic Acid and Inulin in Mice Fed a High-Fat Diet

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RESEARCH PRODUCT

NAFLD and Atherosclerosis Are Prevented by a Natural Dietary Supplement Containing Curcumin, Silymarin, Guggul, Chlorogenic Acid and Inulin in Mice Fed a High-Fat Diet

Marta Di Carlo Pasquale Picone Flavia Mulè Manfredi Rizzo Gaetano-felice Caldara Sara Baldassano Antonella Amato Domenico Nuzzo

subject

0301 basic medicine Male Angiotensinogen Administration Oral Settore BIO/09 - Fisiologia chemistry.chemical_compound Mice 0302 clinical medicine Non-alcoholic Fatty Liver Disease Plant Gums Commiphora chemistry.chemical_classification Nutrition and Dietetics non-alcoholic fatty liver disease; atherogenic lesions; diet-induced obesity; natural dietary supplement; renin-angiotensin system imbalance; Profiler PCR array Angiotensin II Fatty liver Inulin Neoplasm Proteins renin-angiotensin system imbalance 030211 gastroenterology & hepatology atherogenic lesion medicine.symptom Chlorogenic Acid Silymarin medicine.medical_specialty natural dietary supplement Curcumin diet-induced obesity Profiler PCR array; atherogenic lesions; diet-induced obesity; natural dietary supplement; non-alcoholic fatty liver disease; renin-angiotensin system imbalance Inflammation Biology Diet High-Fat Fatty Acid-Binding Proteins Article 03 medical and health sciences Internal medicine Renin–angiotensin system medicine Animals RNA Messenger Profiler PCR array atherogenic lesions Plant Extracts Fatty acid Lipid metabolism medicine.disease Atherosclerosis Lipid Metabolism Mice Inbred C57BL 030104 developmental biology Endocrinology chemistry Gene Expression Regulation Suppressor of Cytokine Signaling 3 Protein Dietary Supplements Curcumin Steatosis Dyslipidemia Food Science

description

Non-alcoholic fatty liver disease (NAFLD) confers an increased risk of cardiovascular diseases. NAFDL is associated with atherogenic dyslipidemia, inflammation and renin-angiotensin system (RAS) imbalance, which in turn lead to atherosclerotic lesions. In the present study, the impact of a natural dietary supplement (NDS) containing Curcuma longa, silymarin, guggul, chlorogenic acid and inulin on NAFLD and atherosclerosis was evaluated, and the mechanism of action was examined. C57BL/6 mice were fed an HFD for 16 weeks; half of the mice were simultaneously treated with a daily oral administration (os) of the NDS. NAFLD and atherogenic lesions in aorta and carotid artery (histological analysis), hepatic expression of genes involved in the NAFLD (PCR array), hepatic angiotensinogen (AGT) and AT1R mRNA expression (real-time PCR) and plasma angiotensin (ANG)-II levels (ELISA) were evaluated. In the NDS group, steatosis, aortic lesions or carotid artery thickening was not observed. PCR array showed upregulation of some genes involved in lipid metabolism and anti-inflammatory activity (Cpt2, Ifng) and downregulation of some genes involved in pro-inflammatory response and in free fatty acid up-take (Fabp5, Socs3). Hepatic AGT, AT1R mRNA and ANG II plasma levels were significantly lower with respect to the untreated-group. Furthermore, NDS inhibited the dyslipidemia observed in the untreated animals. Altogether, these results suggest that NDS prevents NAFLD and atherogenesis by modulating the expression of different genes involved in NAFLD and avoiding RAS imbalance.

year	journal	country	edition	language
2017-05-13	Nutrients

10.3390/nu9050492 http://europepmc.org/articles/PMC5452222