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RESEARCH PRODUCT
Singlet‐Contrast Magnetic Resonance Imaging: Unlocking Hyperpolarization with Metabolism
Stephan KnechtSilvio AimeLaurynas DagysDmitry BudkerFrancesca ReineriGerd BuntkowskyEleonora CavallariCarla CarreraRaphael KircherKerstin MünnemannGinevra Di MatteoMalcolm H. LevittKonstantin L. IvanovJames Eillssubject
Hydrogenchemistry.chemical_elementHyperpolarization; MRI; Metabolism; NMRparahydrogensinglet order010402 general chemistrySpin isomers of hydrogen01 natural sciencesChemical reactionCatalysisNuclear magnetic resonancemedicineMoleculeSinglet stateHyperpolarization (physics)Research Articlesmedicine.diagnostic_test010405 organic chemistryChemistryMagnetic resonance imagingMagnetic Resonance Imaging | Hot PaperGeneral ChemistryMetabolism540NMR0104 chemical sciencesHyperpolarizationMetabolismddc:540Research ArticleMRIdescription
Abstract Hyperpolarization‐enhanced magnetic resonance imaging can be used to study biomolecular processes in the body, but typically requires nuclei such as 13C, 15N, or 129Xe due to their long spin‐polarization lifetimes and the absence of a proton‐background signal from water and fat in the images. Here we present a novel type of 1H imaging, in which hyperpolarized spin order is locked in a nonmagnetic long‐lived correlated (singlet) state, and is only liberated for imaging by a specific biochemical reaction. In this work we produce hyperpolarized fumarate via chemical reaction of a precursor molecule with para‐enriched hydrogen gas, and the proton singlet order in fumarate is released as antiphase NMR signals by enzymatic conversion to malate in D2O. Using this model system we show two pulse sequences to rephase the NMR signals for imaging and suppress the background signals from water. The hyperpolarization‐enhanced 1H‐imaging modality presented here can allow for hyperpolarized imaging without the need for low‐abundance, low‐sensitivity heteronuclei.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-09-10 |