6533b820fe1ef96bd1279c8e

RESEARCH PRODUCT

Beeinflussung des Leberzellmetabolismus, der Freisetzung von reaktiven Sauerstoffspezies sowie Apoptoseaktivierung durch hypotherme oxygenierte Leberperfusion

F. DünschedeTh. JungingerP. DutkowskiA. Krug

subject

chemistry.chemical_classificationReactive oxygen speciesChemistrySuperoxideCold storagemedicine.diseaseMolecular biologyLipid peroxidationchemistry.chemical_compoundImmunologymedicineViaspanLiver preservationReperfusion injuryPerfusion

description

Two different methods of liver preservation were compared : simple cold storage (CS) and hypothermic oxygenated perfusion extracorporal (HOPE). After 10 h of preservation (4°C) with modified UW solution reperfusion was performed by isolated liver perfusion for 90 minutes. Reperfusion injury was estimated by release of cytosolic enzymes, formation of superoxide anions, determination of lipid peroxidation, glycolytic metabolites, bileflow and by PCR analysis. The results showed that after cold storage the formation of reactive oxygen species was significant higher as compared with perfused livers. Correspondingly expressions of mediators (TNFα, NF kappa B, MIP-2, SAPK) and apoptosis (Caspase 9, bax, bak) were detected after CS and were not detectable after HOPE. Additional experiments demonstrated that a short period of hypothermic oxygenation following cold storage (10hCS + 3hHOPE) also could prevent mediator and apoptosis activation. Thus preconditioning of cold injured livers by HOPE is suggested.

https://doi.org/10.1007/978-3-642-55611-1_364