6533b821fe1ef96bd127ba26
RESEARCH PRODUCT
Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications
Nadide AltincekicSophie Marianne KornNusrat Shahin QureshiMarie DujardinMartí Ninot-pedrosaRupert AbeleMarie Jose Abi SaadCaterina AlfanoFabio AlmeidaIslam AlshamlehGisele Cardoso De AmorimThomas AndersonCristiane AnobomChelsea AnormaJasleen Kaur BainsAdriaan BaxMartin BlackledgeJulius BlecharAnja BöckmannLouis BrigandatAnna BulaMatthias BütikoferAldo Camacho-zarcoTeresa CarlomagnoIcaro Putinhon CarusoBetül CeylanApirat ChaikuadFeixia ChuLaura ColeMarquise CrosbyVanessa De JesusKarthikeyan DhamotharanIsabella FelliJan FernerYanick FleischmannMarie-laure FogeronNikolaos FourkiotisChristin FuksBoris FürtigAngelo GalloSantosh GandeJuan Atilio GerezDhiman GhoshFrancisco Gomes-netoOksana GorbatyukSerafima GusevaCarolin HackerSabine HäfnerBing HaoBruno HargittayK. Henzler-wildmanJeffrey HochKatharina HohmannMarie HutchisonKristaps JaudzemsKatarina JovićJanina KaderliGints KalniņšIveta KaņepeRobert KirchdoerferJohn KirkpatrickStefan KnappRobin KrishnathasFelicitas KutzSusanne Zur LageRoderick LambertzAndras LangDouglas LaurentsLauriane LecoqVerena LinhardFrank LöhrAnas MalkiLuiza Mamigonian BessaRachel MartinTobias MatzelDamien MaurinSeth McnuttNathane Cunha Mebus-antunesBeat MeierNathalie MeiserMiguel MompeánElisa MonacaRoland MontserretLaura Mariño PerezCeline MoserClaudia Muhle-gollThais Cristtina Neves-martinsXiamonin NiBrenna Norton-bakerRoberta PierattelliLetizia PontorieroYulia PustovalovaOliver OhlenschlägerJulien OrtsAndrea Da PoianDennis PyperChristian RichterRoland RiekChad RienstraAngus RobertsonAnderson PinheiroRaffaele SabbatellaNicola SalviKrishna SaxenaLinda SchulteMarco SchiavinaHarald SchwalbeMara SilberMarcius Da Silva AlmeidaMarc Sprague-piercyGeorgios SpyrouliasSridhar SreeramuluJan-niklas TantsKaspars TārsFelix TorresSabrina TöwsMiguel TreviñoSven TrucksAikaterini TsikaKrisztina VargaYing WangMarco WeberJulia WeigandChristoph WiedemannJulia Wirmer-bartoschekMaria Alexandra Wirtz MartinJohannes ZehnderMartin HengesbachAndreas SchlundtMiguel Á. Treviñosubject
Life sciences; biologySARS-COV-2; COVID-19; protein production; structural biology NMR[SDV.BIO]Life Sciences [q-bio]/BiotechnologyBiochemistry Genetics and Molecular Biology (miscellaneous)BiochemistryAccessory proteinsNMR spectroscopyddc:570[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]Molecular Biosciencesddc:610Nonstructural proteinsMolecular BiologyOriginal Research[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM]SARS-CoV-2Intrinsically disordered regionnonstructural proteinsCOVID-19structural proteinsCell-free protein synthesisintrinsically disordered regioncell-free protein synthesisaccessory proteins[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/VirologyStructural proteinsdescription
The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-05-10 |