DNA ploidy and S-phase fraction, but not p53 or NM23-H1 expression, predict outcome in colorectal cancer patients. Result of a 5-year prospective study.

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RESEARCH PRODUCT

DNA ploidy and S-phase fraction, but not p53 or NM23-H1 expression, predict outcome in colorectal cancer patients. Result of a 5-year prospective study.

G. Dardanoni Nello Grassi Fabio Fulfaro Valentina Calò Rossana Porcasi Luisa Dusonchet Antonella Amato R. Nuara Federica Latteri Antonio Russo Manuela Migliavacca Nicolo' Gebbia Corsale S Carla Tubiolo Patrizia Cammareri Viviana Bazan Aldo Gerbino Sergio Salerno Vincenza Morello Maria Rosaria Valerio Ines Zanna Rosa Maria Tomasino

subject

Oncology Cancer Research medicine.medical_specialty Pathology Flow-cytometric variable Time Factors Tumor suppressor gene Colorectal cancer Prognosi Settore MED/06 - Oncologia Medica Colon Rectum Biology Adenocarcinoma Disease-Free Survival S Phase Predictive Value of Tests Internal medicine medicine Biomarkers Tumor Humans Stage (cooking)Prospective cohort study Monomeric GTP-Binding Proteins Neoplasm Staging TP53 expression Hematology Ploidies General Medicine DNA Neoplasm Cell cycle NM23 Nucleoside Diphosphate Kinases medicine.disease Colorectal cancer Adenocarcinoma Mucinous Immunohistochemistry Survival Analysis medicine.anatomical_structure Treatment Outcome Oncology Nucleoside-Diphosphate Kinase Immunohistochemistry Lymph Nodes Tumor Suppressor Protein p53 Colorectal Neoplasms Cell Division Transcription Factors

description

Purpose: The aim of this study was to determine TP53 and NM23-H1 immunoreactivity, DNA ploidy, and S-phase fraction (SPF) in a series of 160 patients undergoing resective surgery for primary operable colorectal cancer (CRC) and to establish whether these alterations have any clinical value in predicting CRC patients' prognosis. Methods: TP53 and NM23-H1 expressions were evaluated on paraffin-embedded tissue by immunohistochemistry and DNA-ploidy and SPF on frozen tissue by flow-cytometric analysis. Results: The median follow-up time in our study group was 71 months (range 34-115 months). P53 protein expression was associated with distal tumors (P < 0.05) and DNA aneuploid tumors (P < 0.05) tumors. DNA-aneuploidy was associated with distal tumors (P < 0.01), histological grade (G3) (P < 0.05), advanced Dukes' stage (C and D) (P < 0.01), lymph node metastases (P < 0.01) and high SPF (> 18.3%) (P < 0.01). The major significant predictors for both disease relapse and death were advanced Dukes' stage, DNA-aneuploidy, and high SPF, while lymphohematic invasion was the only independent factor for relapse and non-curative resection for death. Conclusions: Our results indicate that DNA aneuploidy and high SPF are associated in CRC with a poor clinical 5-year outcome, while in contrast the prognostic role of TP53 and NM23-H1 expression is still to be clarified.

year	journal	country	edition	language
2002-12-01	Journal of cancer research and clinical oncology

10.1007/s00432-002-0394-6 https://pubmed.ncbi.nlm.nih.gov/12474051