0000000000044527
AUTHOR
Aldo Gerbino
I tessuti del dente
Expression and localization of MMP-9, MMP-2 and iNOS in some sample of human dental pulp orthodonticaly treated.
Immunohistochemical and molecular expression of CB1 receptors in human colonic segment. Preliminary results
Recent studies document that CB1 cannabinoid receptors, considered since its first identification as “brain specific”, could be expressed by peripheral tissues targets (adipose organs, enteric nervous system, striated muscle, epatocytes). This receptors were found in the GI tract of different species, including mice, rats, guinea pigs, pigs and humans. Endocannabinoids play a role in the pathophysiology of the gastrointestinal tract. They play a role in emesis, acid-related disorders, motility-related disorders, irritable bowel syndrome and diarrhea, and in inflammatory bowel disease. Endocannabinoid system might be involved in cancer differentiation, growth and cell migration. Distribution…
DNA aneuploidy, high SPF and specific p53 mutations are independent prognostic factors in colorectale cancer (CRC)
Parker Willis e l'eccentrica umana passione del Barone Pisani
In Naive mice in vivo localization of WI specific CD8+CD28- induced cells
DNA-aneuploidy and high SPF and specific p53 mutations are independent prognostic factors in colorectal cancer (CRC).
Isolated non-compaction, stroke and trabecular meshwork fibrosis
Expression of Cyclooxygenase-1 and Cyclooxygenase-2 in normal and pathological human oral mucosa
Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is strongly expressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosal reactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positive outcome of oral cancer; therefore it would be useful to identify molecular markers whose expr…
Cordon ombilical. Nouvelles sur l’acétylcholine
Introduction L’acetylcholine (Ach) fut la premiere substance impliquee dans la transmission synaptique. Elle est synthetisee a partir de l’enzyme acetyl-choline transferase (ChAT) et est degradee par l’acetyl-choline esterase (AchE). Il existe des anticorps specifiques diriges contre la ChAT et l’AchE qui ont permis de mettre en evidence la distribution de ce neurotransmetteur au niveau des systemes nerveux central et peripherique. Il faut rappeler que les neurones somato-moteurs, pre-ganglionnaires du tronc cerebral et de la moelle epiniere, les neurones post-ganglionnaires visceraux des ganglions parasympathiques et de quelques ganglions orthosympathiques sont cholinergiques. Ces donnees …
Campailla: materia dei sogni e ipocondria di un eclettico
TP53 Mutation in exon 5 and S-Phase Fraction but not Mutations in Ras gene family and DNA-ploidy are Indipendent prognostic indicators in Laryngeal Squamous Cell Carcinoma
Immunohistochemical expression of Cytokeratins in periapical lesions
Objective. This study seeks to clarify the histological origin of apical lesions, through the expression of certain cytokeratins.Study design. In 3 years 30 patients were selected. After clinical and radiological exams were chosen patients who had severe apical lesions and tooth decay with exposure of the pulp chamber left untreated for very long periods (more the 12 months). Serial slides were prepared both for immunohistochemistry and Hematoxylin- Eosin. Material and methods. Twenty specimens coming from the 30 patients were used for our purpose. All periapical lesions were surgically extracted and fixed in Bouin mixture, and embedded in paraffin. Samples were processed immunohistochemica…
Tessuti del dente e parodonto
Role of Matrix Metalloproteinases 2,9 (MMP-2,-9), Orphanin FQ and Others Peptides in Vascular Tone of Human Umbilical Cord at Term
Umbilical cord is the structure that connects the fetus to the placenta. Umbilical cord at term is made of three vessels surrounded by the Wharton's Jelly, a mocous connective tissue rich in glycosaminoglycans, proteoglycans and different types of collagens and other microfibrils, which are responsible for its elasticity and its mechanical properties. Umbilical cord seems to lack any capillaries or lymphatics and umbilical vessels are unique in lacking any innervation of the vascular tone, and the expression of some enzymes responsible for the remodelling of the extracellular matrix. Trough immunihistochemistry we investigated the expression of matrix metallo-peptidase 2 and 9 (MMP-2, MMP-9…
Atrial Natriuretic Peptide presence in parotid gland of human fetus at 13th week of development and in adult man
Our immunohistochemical study shows that atrial natriuretic peptide (ANP) is present in both human fetal and adult parotid gland. In the fetuses ANP is localized in cell clusters, while in adult parotid gland, the ANP is seen only in the wall of intra- and extralobular ducts. The results suggest that ANP might play a role in the differentiation of the parotid gland similarly as in brain and that during fetal growth ANP may play a role in the regulating the secretion of primitive salivary fluid, even if acini are still not developed. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 55-58)
"Del commercio cogli uomini". La 'Frusta' di Baretti contro Cocchi
Pitré, dalla sostanza delle passioni. Tre cammei di scienza e d'arte: Giovanni Gorgone, Giovanni Meli e Gianfilippo Ingrassia
Expression of Orphanin FQ in Mature Mucous Tissue
Role of K-RAS and P53 Gene in Salivary Glands Tumorigenesis
MMP-2, MMP-9, and iNOS Expression in Human Dental Pulp Subjected to Orthodontic Traction
Abstract Objective: To test the hypothesis that some metalloproteinases (MMP-2, MMP-9) and inducible nitric oxide synthetase (iNOS) enzymes in dental pulp samples do not vary when subjected to orthodontic treatment. Materials and Methods: Human dental pulps were taken from male and female patients (N=10; age 10–14 years). A straight wire technique was used with nickel-titanium or steel archwires. The increase of pressure applied on teeth was gradual. Five patients were subjected to premolar extractions after 14 months of treatment and one after 24 months. Samples were Bouin-fixed, paraffin-embedded, and afterwards processed for immunohistochemistry using anti-MMP-2, anti-MMP-9, and anti-iNO…
"Eccelso mattone della vita". Da Nippur all'embrione della "Scuola di Gundaysabur" al "Poema della Medicina"
I Mitocondri
Giovanni Filippo Ingrassia: A five-hundred year-long lesson.
Giovanni Filippo Ingrassia was born five centuries ago in Regalbuto, a small town in the center of Sicily. After his medical course in Padua, under the guidance of Vesalius and Fallopius, he gained international fame as a physician and was recruited as a Professor of human anatomy in Naples and later in Palermo. He is remembered as "the new Galen" or "the Sicilian Hippocrates." He contributed to the knowledge of human anatomy through the description of single bones rather than the whole skeleton. In particular, he was the first to describe the "stapes," the "lesser wings of the sphenoid" and various other structures in the head (probably the pharyngotympanic tube) as well as in the reproduc…
Immunohistochemical Localization of Epithelial, Endothelial and Muscular Cell Differentiation Markers in Human Umbilical Cord
Introduction. Since time [1], our group studies through immunohistochemistry and RT-PCR analysis the expression of many genes and the localization of the corresponding proteins in the human umbilical cord [2], with the aim to create a map of the presence of the different molecules in the different territories of this organ. In this work we investigate the expression and localization of several cell differentiation markers in the different territories of the human umbilical cord. Materials and methods. The presence of E-Cadherin, CD31, CD68, AE1-AE3, Desmin, Actin ML, HHF35 and MIB-1 was studied through immunohistochemistry. Results. The expression of E-Cadherin (epithelial cell marker) has …
Psychopathia Criminalis, veemenza e disumanizzazione. Presenza italiana di Oskar Panizza
Multiple Supernumerary Teeth with no Other Associated Diseases or Syndromes:Case Report
Cytokeratins identification in human Umbilical Cord at Term
Modulation of MMP-2 and MMP-9 in Churg-Strauss syndrome respiratory mucosa: potential monitoring parameters.
Churg-Strauss (CSS) syndrome is rare and of unknown etiology. It is associated with vasculitis, blood eosinophilia and granulomatosis, and affects multiple organs and systems at various stages of the disease. Specific diagnostic and monitoring tests are not yet available. This study aims to assess the changes in MMP-2 and MMP-9 along with the histopathological alterations in two cases of CSS, as possible potential diagnostic and monitoring criteria. Two adult male patients were diagnosed with CSS in the otorhinolaryngology clinic in the University of Palermo, based on multiple clinical and histopathologic criteria. Biopsies of respiratory mucosa were taken after the consent of the patients…
The prognostic role of TP53 mutations in advanced laryngeal squamous cell carcinoma (LSCC).
HGF-Induced Branching in MDCK Cells is Dependent on the Activation of MEK1 and the Inhibition of GSK-3
Clinical Anatomy and information technology.
Aflame Face. The Sicilian Days of Carlo Levi.
The seventy-three years of Carlo Levi’s (Turin 1902-Rome 1975) arch of life reveal his extraordinary coherency as an intellectual, his political flame, and his aesthetic versatility. His life was an expressive symbiosis with painting and writing: two forms of 'saying' amalgamated as one. If his love for Lucania is distilled from his journey to the South of Italy, his 'Sicilian days', framed in the Fifties, and preserved in his novel Words are Stones, radiate a subterranean and solar Sicily inhabited by violence and by Guttuso’s palette. The absorbing dynamism of the oxymoron governs the whole: that is, a spiritual, geological, social soul of a world, the peasant one, on the threshold of dis…
Expression of Nitric Oxide Synthase in Inflamed Gingival Tissue: Immunoistochemical and Image Analysis
Maxillary Bone Necrosis Following the Use of Formaldehyde-Containing Paste: Three Case Reports
Immunohistochemical expression of apoptotic factors, cytokeratins, and metalloproteinase-9 in periapical and epithelialized gingival lesions
Bellmann K, 2010, CELL STRESS CHAPERON, V15, P101, DOI 10.1007-s12192-009-0126-9; Cappello Francesco, 2011, Front Biosci (Schol Ed), V3, P341, DOI 10.2741-s155; Cappello F, 2006, CANCER, V107, P2417, DOI 10.1002-cncr.22265; Cappello F, 2002, EUR J HISTOCHEM, V46, P199; Carneiro E, 2009, ORAL SURG ORAL MED O, V107, P127, DOI 10.1016-j.tripleo.2008.07.030; Chandra D, 2007, J BIOL CHEM, V282, P31289, DOI 10.1074-jbc.M702777200; Fujita Y, 2011, ODONTOLOGY, V100, P215; Garcia Celia Carrillo, 2007, Med Oral Patol Oral Cir Bucal, V12, pE585; Garcia CC, 2009, ORAL SURG ORAL MED O, V107, pE43, DOI 10.1016-j.tripleo.2008.12.002; Gregory CD, 2011, J PATHOL, V223, P177, DOI 10.1002-path.2792; Gupta S, …
"L'informatione de Pestifero et Contagioso Morbo" di G. F. Ingrassia: Percorso diagnostico.
Comparative analysis of Hsp10 and Hsp90 in large bowel healthy mucosa and adenocarcinomas
Heat shock proteins (Hsps) are an important class of molecules with various functions. Their classic role is to assist other proteins in folding and re-folding and, when proteins are defective or irreversibly misfolded, to drive their degradation. For this reason, some Hsps are also named molecular chaperones. During evolution, this class of proteins has also acquired extrachaperoning roles such as participation in immune system regulation, cell differentiation, programmed cell death and carcinogenesis. Hsp10 is a partner of Hsp60 in the Hsp60/10 folding machine, but numerous scientific studies have shown that Hsp10 may also play other roles. In fact, Hsp10 seems to have an immunomodulatory…
Una nuova ermeneutica del corpo
Bovine seminal vesicles ANP, Oxytocin, immunohistochemical expression.
Expression of angiogenic regulators, VEGF and leptin, is regulated by the EGF/PI3K/STAT3 pathway in colorectal cancer cells.
Both leptin and vascular endothelial growth factor (VEGF) are growth and angiogenic cytokines that are upregulated in different types of cancer and have been implicated in neoplastic progression. Here we investigated the molecular mechanism by which leptin and VEGF expression are regulated in colon cancer by epidermal growth factor (EGF). In colon cancer cell line HT-29, EGF induced the binding of signal transducer and activator transcription 3 (STAT3) to STAT3 consensus motifs within the VEGF and leptin promoters and stimulated leptin and VEGF mRNA and protein synthesis. All these EGF effects were significantly blocked when HT-29 cells were treated with an inhibitor of the phosphoinositide…
Il carro dei poveri
Archivi,scenari della biocomunicazione
A novel use of type 1 collagen gels in determinino the metastatic potential of tumor cells
DNA ploidy and S-phase fraction, but not p53 or NM23-H1 expression, predict outcome in colorectal cancer patients. Result of a 5-year prospective study.
Purpose: The aim of this study was to determine TP53 and NM23-H1 immunoreactivity, DNA ploidy, and S-phase fraction (SPF) in a series of 160 patients undergoing resective surgery for primary operable colorectal cancer (CRC) and to establish whether these alterations have any clinical value in predicting CRC patients' prognosis. Methods: TP53 and NM23-H1 expressions were evaluated on paraffin-embedded tissue by immunohistochemistry and DNA-ploidy and SPF on frozen tissue by flow-cytometric analysis. Results: The median follow-up time in our study group was 71 months (range 34-115 months). P53 protein expression was associated with distal tumors (P < 0.05) and DNA aneuploid tumors (P < …
Expression of angiogenic regulators. VEGF and leptin, is regulated by the EGF/P13K/STAT3 pathway in colorectal cancer cells
New frontiers in regenerative medicine in cardiology: the potential of Wharton's jelly mesenchymal stem cells.
Cardiomyopathies are still the first cause of death in the world. The identification of resident stem cells, comprising those derived from sub-endocardial stroma, suggests the possible self regeneration of the heart under autocrine/paracrine modulation in the cardiac microenvironment. Nevertheless, because of the limited in vivo regeneration potential of damaged cardiac tissue, the use of drugs and ultimately cardiac transplantation remain the common treatments of heart diseases and defects. The differentiative potential of embryonic and mesenchymal stem cells (MSCs) derived from different tissues (such as bone marrow and adipose tissue) was extensively explored in cell therapy for regenera…
Expression of MMP-2 and MMP-9 in odontogenic myxoma in a child: report of a clinical case
Odontogenic myxoma (OM) is a benign, locally invasive, non-metastasizing neoplasm of the jaw bones. Despite the benign nature of these lesions, there is a high rate of recurrence and the current recommended therapy, depending on the size and behaviour of the lesion, can vary from curettage with peripheral ostectomy, segmental resection up to radical resections for more aggressive lesions. OM is a rare tumour which occurs predominantly in the third decade of life and it is rare in children. Matrix metalloproteinases (MMPs) are a family of extracellular endopeptidases responsible for the degradation and remodelling of extracellular matrix, they are known to be involved in the progression and …
Uman Umbilical Cord: Potency of the Cellular Microenvironment
Human Umbilical Cord: Potency of the Cellular Microenvironment
Immunohistochemical Expression of NO-synthase Constitutive Isoform (cNOS)in Mammals Normal Prostate and in Human Prostatic Obstructive Hyperplasia
The aim of present study is to investigate the immunohistochemical identification of cNOS-containing prostatic structures in various species and in hyperplastic human prostate. We used specimens of rat, guinea pig and hamster. Human prostatic specimens were removed from patients suffering of prostatic hyperplasia. The study was processed by ABC method. Immunohistochemical staining with anti nNOS antibody revealed the existence of strong reactivity in numerous ganglionic cell bodies and varicose nerve fibers in the fibromuscular stroma, in smooth cells around acini and in blood vessels endothelium. Surprisingly nNOS immunoreactivity appears in the glandular epithelium. In the hyperplastic pr…
Movimento, motivi bioevoluzionistici
Gists eredo familiari e pediatrici: aspetti biomolecolari e clinici.
Immunohistochemical and Biomolecular Identification of Orphanin FQ, eNOS, Atrial natriuretic Factor and Oxytocin in Rat Seminal Vesicles
In previous studies performed on rodents, we detected the presence of adreno-cholinergic and peptidergic innervation in seminal vesicles and other organs of the male genital system, such as prostate and ductus deferens, in which we also investigated the expression of NOS and NADPH-diaphorase. During the present project we focused our attention on the expression of some peptides involved in local control of smooth muscle relaxation, contractility, vasodilatation and control of blood flow in rat seminal vesicles. We investigated, through immunohistochemistry and RT-PCR, the presence of four peptides: orphanin, eNOS, ANF and oxytocin. Immunohistochemistry was used to detect the presence of the…
Expression of gelatinases and cycloxygenases in one case of myoepithelioma
Expression of Nitric Oxide Synthase in inflamed gingival tissue: immunohistochemical and image analysis
We have used 12 Epulis taken during surgical operation and as control, some normal gingival specimen. The aim was to clarify the relationship between the degree of inflammation and NO production in several cases of Epulis, exhibiting different degree of inflammatory process and verifying the presence of cells involved in NO production. From the immuhistochemical data in our possession we can conclude that NO is one of the factor which module the pathogenecity degree of Epulis.
Mesenchymal Stromal Cells From Wharton's Jelly (WJ-MSCs)
Abstract Among the potential sources of cells with attractive features for regenerative medicine, perinatal tissues have gained much attention in recent years. The umbilical cord (UC) is a fundamental part of placenta, the organ providing feto–maternal interface during pregnancy. The mature UC is constituted by three vessels, one vein, and two arteries, which are comprised into a meshwork formed by a mature mucous connective tissue, named Wharton's jelly (WJ). Recent data from research groups worldwide highlighted that WJ stromal cells possess unique features both in terms of differentiative ability and immunomodulation. These features are desirable for cellular therapy applications, thereb…
Nouvelle utilisation du Collagène de Type I dans la Détermination du potentiel metastatique des Cellules Tumorales : Etude Morphologique et Biochimique
Atrial natriuretic peptide (ANP) and oxytocin-expression in the adult rat and mouse cerebellum.
Abstract Background: Many studies are in the literature on the ANP and oxytocin-presence in the brain, but very few studies with controversial results are reported on the presence of these peptides in the cerebellum. This immunohistochemical study investigates on the ANP and oxytocin-presence in the cerebellum of the adult rat and mouse rodents. Results: This study, firstly, evidences the ANP- immunopositivity in cerebellar cortex of both rat and mouse rodents. In rat the molecular layer presents some few immunopositive fibers, but no neuron resulted immunopositive; the granular and Purkinje cells are immunopositive. In mouse the cerebellar cortex ANP-immunopositivity is present in all laye…
Le Granulome éosinophile des maxillaires: une zone radiotransparente qui n'est pas toujours pathognomique d'une véritable pathologie
Le Granulome éosinophile des maxillaires: une zone radiotransparente qui n’est pas toujours pathognomonique d’une véritable pathologie.
Modification of the peptides ANP and oxytocin in the rat hypothalamic paraventricular nucleus during the physical exercises.
Presence of ANP in internal carotid artery (ICA) with and without atherosclerotic lesions
Mandibulary Eosinophilic Granuloma: Case Series
Eosinophilic granuloma is a proliferation of Langerhans cells in the bones that most commonly involving the skeletal system. The disease generally affects children or young adults with predominance in males and it is characterized by a single or multiple skeletal lesions. This study describes a few cases of mandibular Eosinophilic Granuloma and the effects of surgical curettage of lesions
Il "giuramento di G. F. Ingrassia nella peste di Palermo del 1575 nel suo 500° genetliaco
L'autore pone una riflessione sul giuramento nell'attività medica sia dal punto di vista etico che deontologico. Richiama il solenne impegno di Ingrassia nella tutela della città di Palermo colpita dalla peste e discute i punti di attualità.
Le remaniement de la Matrice Métallo-Proteinase est impliquée dans l'Odontogènese? Etude du MMP-9
...Scienza, qualche osservazione
Expression of beta-Endorphin and Caspase-9 in orthodontically treated dental pulp
ANP presence in the parotid gland in human embryo at 13° week of development.
Expression of gelatinases (MMP-2, MMP-9) and cyclooxygenases (COX-1, COX-2) in some benign salivary gland tumors.
Salivary gland tumors, most of which are rare benign tumors, represent a histologically heterogenous group with the greatest diversity of morphological and cellular features. The aim of this study is to analyse the expression and possible interactions between gelatinases (MMP-2, MMP-9) and cyclooxygenases (COX-1, COX-2) in some benign salivary gland tumors. We investigated the expression of gelatinases and cyclooxigenases in control salivary gland, Pleomorphic adenoma and Warthin's tumor through immunohistochemistry and Reverse Transcription – Polymerase Chain Reaction (PCR). We identified the expression of both classes of enzyme in normal samples and in the two types of pathological sampl…
I tumori della mammella e/o dell’ovaio di tipo eredofamiliare: strategie di prevenzione nelle donne ad alto rischio
Numerosi fattori sono stati coinvolti nell’insorgenza del carcinoma mammario e dell’ovaio tra cui l’età, fattori ormonali e riproduttivi come l’età e il numero di gravidanze e l’utilizzo di contraccettivi orali e, in minor misura, fattori ambientali e stili di vita. Le possibilità per la gestione clinica delle pazienti considerate ad alto rischio di sviluppare carcinoma della mammella e/o dell’ovaio includono chirurgia profilattica (es. mastectomia bilaterale profilattica o ovarosalpingectomia bilaterale), chemioprevenzione (principalmente tamoxifene e raloxifene ma anche, più recentemente, inibitori dell’aromatasi nelle donne ad alto rischio in postmenopausa), cambiamenti nelle abitudini d…
Il pensiero biologico
The Bio-Molecular Dynamics of Dental Pulp in Different Clinical Scenarios
Dental pulp (DP) is a very dynamic tissue both in health and in disease. When exposed to stressors and pathological conditions. It undergoes a complex series of biological reactions whereby alterations affect the pulp tissue at tissue cellular and molecular levels. The aim of this review is to update the reader on the various bio-molecular alterations in the dental pulp under different clinical conditions: orthodontic treatment (OT), caries, pulpitis and others. The morphological changes in the composition of the DP rang from the reversible remodeling to apoptosis and sometimes necrosis. Many apoptotic factors are involved like Bcl2, Bax and the significant increase in Caspases 9 and 3, as …
MEK1 Required for Invasive Growth of mIMCD3 renal Cells in a 3D Collagen Matrix
Branching morphogenesis is an essential process during kidney development: This process controls epithelialization of the metanephric mesenchyme during the induction of branching structures and in this way determines the number of nephrons. Various grow factors, such HGF, have been implicated in branching morphogenesis in renal cells, including mIMCD3 ( murine inner medullary collecting duct) and MDCK cells. Growth factors which induce branching morphogenesis in renal cells also activate the MEK1/ERK pathway. The authors analyzed the potential role that this pathway might play in branching morphogenesis. The authors show that PD98059, a specific inhibitor of MEK1,inhibits branching of mIMCD…
S2-P114 MAXILLARY BONE NECROSIS FOLLOWING THE USE OF FORMALEHYD-CONTAINING PASTE. THREE CASE REPORTS
Carcinoma ereditario dell'ovaio (Ereditary Ovarian Cancer)
Gemma Dentale: studio immunoistochimico
The neurosecretion of oxytocin in the hypothalamic magnocellular nuclei of training rats
Preliminary observations on the immunohistochemical distribution of nNOS in the development of human foetus eye
Histochemical and ultrastructural remarks of the urinary tract in some mammals.
TWENTY YEARS' HISTORY OF IMMUNOHISTOCHEMICAL MAMMALS DENTAL PULP STUDIES
CB1 receptor expression in pancreatic islet of the obese and diabetes Zucher rats
Nadph Diaphorase Histochemical Presence in some Mammal's Penis
Intrabdominal and extrabdominal portion of penis (rat, guinea pig, hamster and rabbit) have been used for histochemical activity Nadph-diaphorase. Pharmacological and electrophysiological studies, both in vivo and in vitro, make us to belive that, in man, dog, rabbit and rat, the relaxation of vascular smooth muscle of the cavernous body of penis, that is responsible for erection, is produced by the activation of a Non-Adrenergic, Non-Cholinergic (NANC) nervous pathway whose mediator is a new neurotrasmitter: Nitric Oxide (NO). Our observations provide the morphohistochemical base for the localization of NADPH-diaphorase in the different constitutive structures of the penis in some mammalia…
TP53 and p16INK4A, but not H-KI-Ras, are involved in tumorigenesis and progression of pleomorphic adenomas.
The putative role of TP53 and p16INK4A tumor suppressor genes and Ras oncogenes in the development and progression of salivary gland neoplasias was studied in 28 cases of pleomorphic adenomas (PA), 4 cases of cystic adenocarcinomas, and 1 case of carcinoma ex-PA. Genetic and epigenetic alterations in the above genes were analyzed by Polymerase Chain Reaction/Single Strand Conformational Polymorphism (PCR/SSCP) and sequencing and by Methylation Specific-PCR (MS-PCR). Mutations in TP53 were found in 14% (4/28) of PAs and in 60% (3/5) of carcinomas. Mutations in H-Ras and K-Ras were identified in4%(1/28) and7% (2/28) of PAs, respectively. Only 20% (1/5) of carcinomas screened displayed mutatio…
Imaging ecografico del cordone ombelicale umano.
Human Umbilical Cord at Term. Immunohistochemical expression of MMP-2, MMP-9, Orphanin F/Q and others Peptides involved in the regulation of vascular tone
B-Endorphin in Human Dental Pulp During Orthodontic Treatments
Imaging ecografico del cordone ombelicale umano Ecographic imaging of the human umbilical cord
TP 53, H-K-Ras, P16INK4A gene molecular analysis in salivary gland tumors.
Presence in trigeminal ganglia in developing rat.
ANP (Atrial Natriuretic Peptide) presence in the heart of a tunicate, Ciona intestinalis.
Atrial natriuretic peptide was found in the heart of vertebrates, we studied the ANP presence in the heart of Ciona intestinalis. This is animal is very important because of the its evolutionary position between invertebrates and vertebrates. ANP presence was only revealed in myoepithelial cells of the myocardium. Results suggest the hypothesis that ANP is present not only in the vertebrates but also in the invertebrates and in Ciona heart ANP might play a similar role like in the heart of vertebrates.
Immunohistochemical and transcriptional expression of the matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa
The oral cavity is exposed to chronic or recurrent, physical and chemical trauma that could lead to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). The objective of this study is to investigate the expression and the possible changes of the two matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa samples. Normal oral mucosa samples and three different types of pathological conditions (hyperplasia, dysplasia and carcinoma) were used for this study. Immunohistochemical analysis was used to evaluate protein expression for the two enzymes, while Reverse Transcription ? Polymerase Chain Reaction (RT-PCR) was used to investigate gene expression. Image…
ANP-presence and its relationship with the oxytocin in nonlactanting breast of rabbit
"Fuori bersaglio": Semmelweis, homo tragicus
TP53, H-K-RAS, P16INK4A GENE MOLECULAR ANALYSIS IN SALIVARY GLAND TUMORS
P23-Le remaniement de la Matrice metallo-Proteinase est impliquée dans l'Odontogènese? E'tude du MMP-9
Ildegarda: del corpo, dell'anima
Expression of remodelling enzymes (MMP2-MMP9) in samples of salivary gland's tumors
Immunohistochemical localization of MMP-9 and Orphanin in some sample of dental pulp.
CORDON OMBILICAL.NOUVELLES SUR L'ACETYLCHOLINE
Human umbilical cord expresses several vasoactive peptides involved in the local regulation of vascular tone: protein and gene expression of Orphanin, Oxytocin, ANP, eNOS and iNOS.
Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton&#8217;s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. The aim of this study was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). The expression of these molecules in full-term human umbilical cord …
GIOVANNI GORGONE" THE NESTOR OF ANATOMISTS"
Orthodontic Stress BCL-2 Nodulation and Human Odontoblast's Survival
Expression of cytokeratins in human umbilical cord at term
Background. Cytokeratins are the proteins that costitute intermediate filaments. In the cytoplasm, keratin filaments extend from the nucleus to the plasma membrane. This link is important for the structural organization of the cytoplasm and for many cellular processes such as mitosis, cell movement, cell differentiation and adhesion. The pattern of expression of cytokeratins is frequently organ or tissue specific and depends mainly on the type of epithelium, the level of differentiation and the stage of development. The expression of cytokeratins in umbilical cord has been investigated by many groups, but these previous studies gave only fragmentary informations: each single study focused o…
IL GRANULOMA CENTRALE A CELLULE GIGANTI DEI MASCELLARI
Central giant cell granuloma ( CGCG ), is a fibro-osseous lesion. The majority of lesions are observed in the mandible and mainly in children and young adults, more often in females than in males. This lesion, which the pathogenesis still remains obscure, appears as radiolucency, well or ill-defined, uni or multilocular with trabeculations coursing through the lesion. Histologic study shows giant cells, fibroblastic cells, and foci of hemorrhage and osteoid tissue. On the basis of clinical, radiological and histologic features, central giant cell granulomas can be classified as "non-aggressive" or "aggressive"; the histomorphometric analysis prove a significant increase in large giant cells…
Nel serra serra dei capelli. L'amore in un manipolo di odii
Viene ricordato dopo i duecento anni della morte, il poeta Giovanni Meli. Sono messe in evidenza le anacreontiche che raccontano dell'amore, della bellezza femminile in una nota cornice arcadica.
DNA-Aneuploidy, High SPF and Specific P53 Mutations are Indipendent Prognostic Factors in Colorectal Cancer (CRC)
Expression of Gelatinases (MMP-2, MMP-9) in human articular cartilage
Osteoarthritis (OA) is a chronic degenerative joint disorder characterized by destruction of the articular cartilage, subchondral bone alterations and synovitis. Matrix metalloproteinases (MMPs) are expressed in joint tissues of patients with osteoarthritis (OA). The objective of this study was to define the steady state levels of two different MMPs to provide more insight into the role of MMPs in cartilage destruction in OA. We investigated the expression of gelatinases through immunohistochemistry Our results show that high levels of MMP-2 and MMP-9 are present in OA and suggest that once these MMPs are fully activated they may contribute to the cartilage destruction in OA.
Gorgone, l'occhio e la mano
Wharton’s Jelly Mesenchymal Stromal Cells Support the Expansion of Cord Blood–derived CD34+Cells Mimicking a Hematopoietic Niche in a Direct Cell–cell Contact Culture System
Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) have been recently exploited as a feeder layer in coculture systems to expand umbilical cord blood–hematopoietic stem/progenitor cells (UCB-HSPCs). Here, we investigated the role of WJ-MSCs in supporting ex vivo UCB-HSPC expansion either when cultured in direct contact (DC) with WJ-MSCs or separated by a transwell system or in the presence of WJ-MSC–conditioned medium. We found, in short-term culture, a greater degree of expansion of UCB-CD34+cells in a DC system (15.7 ± 4.1-fold increase) with respect to the other conditions. Moreover, in DC, we evidenced two different CD34+cell populations (one floating and one adherent to WJ-MSCs) with …
Tommaso Campailla: Materia dei sogni e ipocondria di un eclettico
Born on 7th April 1668 in Modica, Ciceronian 'Agrum Motucanum', the Modican county of Chiaramontes and Chiabreras, Tommaso Campailla, a poet and a physician, is mostly known as the author of a philosophical poem in octaves--Adam or the Created World. In his 1727 Problemi naturali [Natural Problems], his essay Del disordinato discorso dell'uomo [On Man's Disorderly Discourse] distinguishes itself by its intellectual wit on both the speculative and the experimental research aspects. He formulates concepts on the darkness which often reason has to fare through; he substantializes the body/soul syncretic dimension, and underlines how this disrupts the perfect bodily mechanism. His observations …
New insight on immunological activation pathways of Langerhans cells, possible tolerogenic role
Langerhans cells are the prototype of antigen presenting cell, their role is to work as sentinel in the epidermis. Like every APC Langerhans cells act as bridge between innate and acquired immunity recognizing antigens into the epidermis and bringing them to drying lymph node, their work is well described by the Langerhans cell paradigm. Recently many works designed a new and amazing role of Langerhans cells in fact they often showed tolerogenic capacity, while in many cases, they seems not necessary to promote activation of acquired immunity. Anyway Langerhans cells remaining an interesting target for new vaccine strategies because of their localization in the epidermis and the ability to …
ANP PRESENCE IN THE COROID PLEXUS IN THE LATERAL VENTRICLES IN THE DEVELOPING RAT
Nouvelles utilisations du collagène de type I dans la determination du potentiel métastatique des cellules tumorales: etudes morphologique et biochimique
Magnetic Resonance Spectroscopy: principles and clinical applications
Presence of ANP in the suprachiasmatic nucleus of the developing rat.
Localisation immunohistochemique du factuer natriuretique atriale du tissu muqueux mur du Cordon Ombelical humain après naissance et dans la Pulpe Dentaire.
Histochemical Geography and Ultrastructural Remarks of the Urinary Tract in Some Mammals
Our investigations oriented towards the urinary tracts, particulary the bladder and ureter, were carried out by histochemical and ultrastructural studies by SEM and TEM observation. In this segment of urinary tract, by structural, ultrastructural and chemical analysis, have evinced variations related to different animal species (guinea pig, rabbit and rat). The bladder wall was considered from us for histochemical aspects related to hydrolytic lysosomial enzymes (Ac-Phase; Na-beta-Gase) and oxidative enzymes (SDH; Iso-DH; MADH) and ultrastructural aspect with TEM. The SEM observations showed the connection between morphovolumetric variations of uroepithelial cells and respiratory enzymatic …
Twenty years' history of immunohistochemical mammals dental pulps studies.
Immunohistochemical and transcriptional expression of matrix metalloproteinases in full-term human umbilical cord and Human Umbilical Vein Endothelial Cells
Matrix metalloproteinases (MMPs) are extracellular zinc-dependent endopeptidases involved in the degradation and remodelling of extracellular matrix in physiological and pathological processes. MMPs also have a role on cell proliferation, migration, differentiation, angiogenesis and apoptosis. Umbilical cord is a special organ subjected to many changes during pre-natal life and whose cells can maintain a certain degree of plasticity also in post-natal period; for example recently they have been used as a source of stem cells. In this work we investigated the expression of MMPs in human umbilical cord and Human Umbilical Vein Endothelial Cells (HUVEC) though immunohistochemistry, RT-PCR and …
Functional Aspects of Adrenocholinergic and Peptidergic Innervation about Prostate and Seminal Vesicles in Mammals: A Review
This study was aimed to explain the functional role of mediation in the organs of the male reproductive system (prostate and seminal vesicles of rat and hamster), representing throught the visualization of adrenergic fibres (Falck Method), cholinergic fobres (Martinez-Rodriguez method) and by immunohistochemical methods to detect the P substance and the CGRP (PAP) in glandular structures. The adrenocholinergic and peptidergic mediators have a growing interest for their synergic action in the control of contractility, vasodilatation and most likely, of the secretory activity of the epithelium, probably expressed by andocrine-paracrine mechanisms.
Expression of Remodelling Enzymes (MMP-2, MMP-9) in Normal and Pathological Oral Mucosa Samples
Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 th…
Embriologia del collo e del mediastino superiore
ANP presence in the choroid plexus in the lateral ventricles in the development rat
COX- Expression in the inflamed gingival tissue:Immunohistochemistry,Cytochemistry and Biochemistry test.
Prostaglandins (PG) are chemical mediators for most physiological and pathological responses ,including cytoprotection of the gastric mucosa, blood coagulation,inflammation and pain (1,2,3). E2 Prostaglandins (PG2) play an important role of pathogenesis of periodontal diseases, since PG2 determines vasodilatation and increased vascular permeability, oedema and erythema.Therefore,it is not surprising to find high levels of PG2 in inflamed gingival tissues.Indeed ,in inflamed gingival tissue,PG2 levels of the Gingival Fluid (GCF) are known to be higher in periodontitis than in healthy gingiva.Moreover ,in different parodontitis the high PG2 level of the GCF is related to periodontal ligament …
The liver: three-dimensional geometrical models. About a new stereology.
Expression of Remodelling Enzymes MMPs in Hernia Formation
ANP Presence in trigeminal ganglia in developing rat
Cell differentiation markers in human umbilical cord; an immunohistochemical study
The full-term human umbilical cord is made of three blood vessels (two arteries and a vein) surrounded by the Wharton’s jelly, a soft connective tissue made of few mesenchymal stromal cells and an abundant extracellular matrix. Both endothelial and Wharton’s jelly cells are versatile in their differentiation potential; they have shown to be able to differentiate into several cell lineages. Since the differentiation potential of these cells is very wide, we thought it could be interesting to investigate the expression of several cell differentiation markers in the different regions of human umbilical cord. Immunohistochemistry showed the expression of E-cadherin and cytokeratins in the amnio…
Vent'anni di storia sullo studio immunoistochimico delle polpe dentali di mammiferi Twenty years'history of immunohistochemical mammals dental pulp studies
Nel corpo, nel paesaggio
Orphanin FQ and motility in seminal vesicles
Expression of matrix metalloproteinases in full-term human umbilical cord and Human Umbilical Vein Endothelial Cells.
Background. Matrix metalloproteinases (MMPs) are extracellular zinc-dependent endopeptidases involved in breakdown and remodelling of extracellular matrix in physiological and pathological processes. MMPs have also a role on cell proliferation, migration, differentiation, angiogenesis and apoptosis. Umbilical cord is a special organ subjected to many changes during pre-natal life and whose cells can maintain a certain degree of plasticity also in post-natal period; for example recently they have been used as a source of stem cells. For these reasons we believe it is interesting to investigate the expression of some remodelling enzymes in this tissue. Human Umbilical Vein Endothelial cells (…
Oxytocin and vasopressin expression in the turbinates of patients with chronic sinusitis
Many peptides are present in the nasal mucosa, but few studies have investigated the presence or absence of the oxytocin and vasopressin peptides. This immunohistochemical study on the inferior turbinates of patients affected by chronic sinusitis shows, for the first time, that these peptides are present in the epithelium of both nasal mucosa and glands. Their presence could be related to the presence of atrial natriuretic peptide (ANP), like previously demonstrated in other organs such as heart and prostate, since in some circumstances they play in antagonism.
1906: Quelle provette a Porta di Mazara
Cellule e tessuti
Motta Pietro
L'Esofago di Barrett
Prefazione alla monografia "L'Esofago di Barrett. Valutazioni personali dell'endoscopista e analisi delle più recenti acquisizioni"