6533b82afe1ef96bd128c39b

RESEARCH PRODUCT

Isoprenaline and forskolin increase evoked vasopressin release from rat pituitary

Erich MuschollMichael RothländerKurt Racké

subject

medicine.medical_specialtyPituitary glandVasopressinVasopressinsStimulationPropranololchemistry.chemical_compoundPituitary Gland PosteriorInternal medicineIsoprenalinemedicineAnimalsPharmacologyForskolinColforsinIsoproterenolRats Inbred StrainsElectric StimulationStimulation ChemicalRatsEndocrinologymedicine.anatomical_structureVasopressin secretionchemistryPituitary GlandTetrodotoxinFemaleDiterpeneshormones hormone substitutes and hormone antagonistsmedicine.drug

description

Isolated neurointermediate lobes of rat pituitaries were incubated in Krebs solution. The vasopressin release evoked by electrical stimulation (0.2 ms, 80 V, 15 Hz, 10 s trains at 10 s intervals for a total of 10 min) was completely inhibited by tetrodotoxin. Isoprenaline increased the evoked vasopressin release to a maximum of 60% (EC50 10 nM) and this effect was antagonized surmountably by propranolol. Forskolin increased the vasopressin release by 98%. These results suggest the presence within the neurohypophysis of a beta-adrenoceptor-linked adenylate cyclase facilitating vasopressin secretion.

yearjournalcountryeditionlanguage
1982-08-13European Journal of Pharmacology
https://doi.org/10.1016/0014-2999(82)90560-x