USE OF FUZZY NEURAL NETWORKS IN MODELING RELATIONSHIPS OF HPV INFECTION WITH APOPTOTIC AND PROLIFERATION MARKERS IN POTENTIALLY MALIGNANT ORAL LESIONS

6533b82bfe1ef96bd128e3b4

RESEARCH PRODUCT

USE OF FUZZY NEURAL NETWORKS IN MODELING RELATIONSHIPS OF HPV INFECTION WITH APOPTOTIC AND PROLIFERATION MARKERS IN POTENTIALLY MALIGNANT ORAL LESIONS

Giuseppina Campisi Giuseppina Capra Lucia Giovannelli Immacolata Greco Ada Maria Florena F. Calvino Olga Di Fede Lorenzo Lo Muzio

subject

Male Cancer Research Oral precancerous lesion Survivin Fuzzy neural networks Apoptosis Polymerase Chain Reaction Inhibitor of Apoptosis Proteins law.invention law Genotype Papillomaviridae Polymerase chain reaction Leukoplakia biology Smoking HPV infection virus diseases Middle Aged female genital diseases and pregnancy complications Neoplasm Proteins Cell Transformation Neoplastic Proto-Oncogene Proteins c-bcl-2 Oncology Carcinoma Squamous Cell Female Mouth Neoplasms Leukoplakia Oral Oral Surgery Microtubule-Associated Proteins Adult HPV Fuzzy Logic Proliferating Cell Nuclear Antigen Survivin Carcinoma medicine Humans Bcl-2 Aged Cell Proliferation Analysis of Variance Papillomavirus Infections Mouth Mucosa medicine.disease Proliferating cell nuclear antigen DNA Viral Immunology biology.protein Cancer research Neural Networks Computer Nested polymerase chain reaction

description

To evaluate in oral leukoplakia the relationship between HPV infection and markers of apoptosis (bcl-2, survivin) and proliferation (PCNA), also conditionally to age, gender, smoking and drinking habits of patients, by means of Fuzzy neural networks (FNN) system 21 cases of oral leukopakia, clinically and histologically diagnosed, were examined for HPV DNA presence, bcl-2, survivin and PCNA expression. HPV DNA was investigated in exfoliated oral mucosa cells by nested PCR (nPCR: MY09-MY11/GP5-GP6), and the HPV genotype determined by direct DNA sequencing. All markers were investigated by means of standardised immunohistochemistry procedure. Data were analysed by chi-square test, crude OR and the 95% Cl; in blindness, FNN was applied. HPV DNA was found in 8/21 OL (38.1%); survivin, PCNA, and tobacco smoking were associated in univariate analysis (p = 0.04) with HPV DNA status. HPV-18 was the most frequently detected genotype (6/8), followed by HPV-16 (2/8). FNN revealed that survivin and PCNA, both being expressed in all of OL HPV+ve, were associated with HPV infection. In conclusion, the FNN allowed to hypothesise a model of specific variables associated to HPV infection in OL. The relevance of survivin and PCNA suggest that they may be involved in HPV-mediated deregulation of epithelial maturation and, conversely, that HPV may have a role in the expression level of these two markers. FNN system seems to be an effective tool. in the analysis of correlates of OL and HPV infection. (c) 2005 Elsevier Ltd. All rights reserved.

year	journal	country	edition	language
2005-11-01

10.1016/j.oraloncology.2005.05.014 http://hdl.handle.net/10447/19668