Immature excitatory neurons develop during adolescence in the human amygdala.

6533b82cfe1ef96bd128f671

RESEARCH PRODUCT

Immature excitatory neurons develop during adolescence in the human amygdala.

José Manuel García-verdugo Dmitry Velmeshev Ricardo Insausti Mercedes F. Paredes Vicente Herranz-perez Vicente Herranz-pérez Edward F. Chang Simone Mayer Kadellyn Sandoval John L.r. Rubenstein Eric J. Huang Shawn F. Sorrells Arnold R. Kriegstein Arturo Alvarez-buylla

subject

0301 basic medicine Male General Physics and Astronomy Hippocampus 02 engineering and technology Adult neurogenesis Hippocampus Neural Stem Cells lcsh:Science Child Pediatric Neurons Multidisciplinary Neuronal Plasticity biology Basolateral Nuclear Complex Q Neurogenesis Middle Aged 021001 nanoscience & nanotechnology Mental Health medicine.anatomical_structure Child Preschool Excitatory postsynaptic potential Single-Cell Analysis 0210 nano-technology Sequence Analysis Adult Ganglionic eminence Adolescent Science Neurogenesis Inhibitory postsynaptic potential Amygdala Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Young Adult Fetus medicine Humans Preschool Progenitor Aged Cell Nucleus Sequence Analysis RNA Infant Newborn Neurosciences Infant General Chemistry Adolescent Development Stem Cell Research Newborn 030104 developmental biology nervous system biology.protein Neuronal development RNA lcsh:Q TBR1 Neuroscience

description

The human amygdala grows during childhood, and its abnormal development is linked to mood disorders. The primate amygdala contains a large population of immature neurons in the paralaminar nuclei (PL), suggesting protracted development and possibly neurogenesis. Here we studied human PL development from embryonic stages to adulthood. The PL develops next to the caudal ganglionic eminence, which generates inhibitory interneurons, yet most PL neurons express excitatory markers. In children, most PL cells are immature (DCX+PSA-NCAM+), and during adolescence many transition into mature (TBR1+VGLUT2+) neurons. Immature PL neurons persist into old age, yet local progenitor proliferation sharply decreases in infants. Using single nuclei RNA sequencing, we identify the transcriptional profile of immature excitatory neurons in the human amygdala between 4–15 years. We conclude that the human PL contains excitatory neurons that remain immature for decades, a possible substrate for persistent plasticity at the interface of the hippocampus and amygdala.

year	journal	country	edition	language
2019-06-01

https://escholarship.org/uc/item/3vn2k1qg