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RESEARCH PRODUCT

Development of a Rapid LC-MS/MS Method for the Determination of Emerging Fusarium mycotoxins Enniatins and Beauvericin in Human Biological Fluids

Susy PiovesanaAnna Laura CapriottiRoberto SamperiChiara CavaliereAldo LaganàSalvatore VenturaA.b. Serrano

subject

AdultMaleElectrosprayHealth Toxicology and Mutagenesislcsh:MedicineToxicologyMass spectrometryTandem mass spectrometryArticleMatrix (chemical analysis)chemistry.chemical_compoundenniatinsFusariumLiquid chromatography–mass spectrometryLimit of DetectionTandem Mass SpectrometrymycotoxinsDepsipeptidesHumansSolid phase extractionliquid chromatography-tandem mass spectrometryplasmaAgedDetection limitChromatographylcsh:RbeauvericinSolid Phase ExtractionMiddle Agedbeauvericin; enniatins; liquid chromatography-tandem mass spectrometry; mycotoxins; plasma; urineBeauvericinurinechemistryFemaleChromatography Liquid

description

A novel method for the simultaneous determination of enniatins A, A1, B and B1 and beauvericin, both in human urine and plasma samples, was developed and validated. The method consisted of a simple and easy pretreatment, specific for each matrix, followed by solid phase extraction (SPE) and detection by high performance liquid chromatography-tandem mass spectrometry with an electrospray ion source. The optimized SPE method was performed on graphitized carbon black cartridges after suitable dilution of the extracts, which allowed high mycotoxin absolute recoveries (76%–103%) and the removal of the major interferences from the matrix. The method was extensively evaluated for plasma and urine samples separately, providing satisfactory results in terms of linearity (R2 of 0.991–0.999), process efficiency (&gt

yearjournalcountryeditionlanguage
2015-09-01Toxins
10.3390/toxins7093554http://europepmc.org/articles/PMC4591648