Poly(N-isopropylacrylamide)-gated Fe3O4/SiO2 core shell nanoparticles with expanded mesoporous structures for the temperature triggered release of lysozyme

6533b82ffe1ef96bd1295289

RESEARCH PRODUCT

Poly(N-isopropylacrylamide)-gated Fe3O4/SiO2 core shell nanoparticles with expanded mesoporous structures for the temperature triggered release of lysozyme

José Ramón Murguía Ramón Martínez-máñez Pedro Amorós Pieter Stroeve Erick Yu Erick Yu María D. Marcos Irene Galiana Félix Sancenón Elena Aznar

subject

INGENIERIA DE LA CONSTRUCCION Silicon dioxide Acrylic Resins Biomedical Engineering Nanoparticle Bioengineering chemistry.chemical_compound PNIPAM QUIMICA ORGANICA Colloid and Surface Chemistry Bacillus cereus BIOQUIMICA Y BIOLOGIA MOLECULAR Nanotechnology Ferrous Compounds Physical and Theoretical Chemistry Chemical Physics Chromatography biology Protein delivery QUIMICA INORGANICA Temperature Triggered release Surfaces and Interfaces General Medicine Chemical Engineering Mesoporous silica Silicon Dioxide biology.organism_classification Anti-Bacterial Agents Micrococcus luteus chemistry Drug delivery Poly(N-isopropylacrylamide)Nanoparticles Muramidase Lysozyme Pore expansion Mesoporous material Micrococcus luteus Porosity Mesoporous silica Physical Chemistry (incl. Structural)Biotechnology Nuclear chemistry

description

Core-shell nanoparticles comprised of Fe3O4 cores and a mesoporous silica shell with an average expanded pore size of 6.07 nm and coated with a poly(N-isopropylacrylamide) (PNIPAM) layer (CS MSNs EP PNIPAM) were prepared and characterized. The nanoparticles was loaded with (Ru(bipy)3 2+) dye or an antibacterial enzyme, lysozyme, to obtain CS MSNs EP PNIPAM Ru(bipy)3 2+ and CS MSNs EP PNIPAM Lys, respectively. The lysozyme loading was determined to be 160 mg/g of nanoparticle. It was seen that Ru(bipy)3 2+ and lysozyme release was minimal at a room temperature of 25 ºC while at physiological temperature (37 º C), abrupt release was observed. The applicability of the CS MSNs EP PNIPAM Lys was further tested with two Gram-positive bacteria samples, Bacillus cereus and Micrococcus luteus. At physiological temperature, the nanoparticles were shown to reduce bacterial growth, indicating a successful release of lysozyme from the nanoparticles. This nanoparticle system shows potential as a nanocarrier for the loading of similarly sized proteins or other species as a drug delivery platform.

year	journal	country	edition	language
2015-11-01	Colloids and Surfaces B: Biointerfaces

https://doi.org/10.1016/j.colsurfb.2015.06.048