Bioanalysis of digoxin and its metabolites using direct serum injection combined with liquid chromatography and on-line immunochemical detection.

6533b830fe1ef96bd1296755

RESEARCH PRODUCT

Bioanalysis of digoxin and its metabolites using direct serum injection combined with liquid chromatography and on-line immunochemical detection.

H. Irth Klaus K. Unger U.r. Tjaden J. Van De Greef M. Beth A.j. Oosterkamp

subject

Detection limit Bioanalysis Analyte Digoxin Chromatography Digoxin medicine.diagnostic_test Metabolite Immunochemistry Fluoroimmunoassay General Chemistry Cross Reactions chemistry.chemical_compound chemistry Immunoassay medicine Digoxigenin Humans Quantitative analysis (chemistry)Digoxigenin Biomarkers Chromatography High Pressure Liquid medicine.drug

description

Abstract An automated dual-column liquid chromatographic assay for digoxin is described. Serum samples are directly injected onto a restricted-access solid-phase extraction support. After liquid chromatographic (LC) separation on a C18 analytical column, antigenic analytes are detected by means of post-column immunochemical detection (ICD) using fluorescein-labelled antibodies against digoxigenin. The detection limit of this assay is 160 pg/ml (preconcentration of 1.0 ml serum). With the present method digoxin and three of its cross-reactive metabolites were determined in serum taken from patients which were orally administered a 1-mg dose of digoxin. The results obtained with LC—ICD were compared with data provided by a batch immunoassay.

year	journal	country	edition	language
1994-02-18	Journal of chromatography. B, Biomedical applications

10.1016/0378-4347(93)e0405-f https://pubmed.ncbi.nlm.nih.gov/8012560

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