Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis.

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RESEARCH PRODUCT

Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis.

Norbert Wikonkál Balázs Mayer Marta Lange Alexey Lihachev Szabolcs Bozsányi Krisztina Becker Judit Noll Pálma Anker Márta Medvecz Kende Lőrincz Ilze Lihacova Enikő Kuroli Norbert Kiss Luca Fésűs

subject

Male Nonlinear Optical Microscopy Hyperkeratosis autofluorescence lcsh:Chemical technology Biochemistry Analytical Chemistry 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine In vivo Keratin medicine Humans lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation keratin Skin chemistry.chemical_classification Hyperkeratosis Epidermolytic integumentary system hyperkeratosis Chemistry Communication Optical Imaging LED nonlinear microscopy epidermolytic ichthyosis medicine.disease diffuse reflectance Fluorescence Nonlinear optical microscopy Atomic and Molecular Physics and Optics Autofluorescence medicine.anatomical_structure 030220 oncology & carcinogenesis Child Preschool multiphoton microscopy histopathology Keratins KRT1 Epidermis Diffuse reflection Biomedical engineering

description

Keratins are one of the main fluorophores of the skin. Keratinization disorders can lead to alterations in the optical properties of the skin. We set out to investigate a rare form of keratinopathic ichthyosis caused by KRT1 mutation with two different optical imaging methods. We used a newly developed light emitting diode (LED) based device to analyze autofluorescence signal at 405 nm excitation and diffuse reflectance at 526 nm in vivo. Mean autofluorescence intensity of the hyperkeratotic palmar skin was markedly higher in comparison to the healthy control (162.35 vs. 51.14). To further assess the skin status, we examined samples from affected skin areas ex vivo by nonlinear optical microscopy. Two-photon excited fluorescence and second-harmonic generation can visualize epidermal keratin and dermal collagen, respectively. We were able to visualize the structure of the epidermis and other skin changes caused by abnormal keratin formation. Taken together, we were able to show that such imaging modalities are useful for the diagnosis and follow-up of keratinopathic diseases.

year	journal	country	edition	language
2021-02-01	Sensors (Basel, Switzerland)

10.3390/s21041105 https://pubmed.ncbi.nlm.nih.gov/33562614