6533b853fe1ef96bd12ad787
RESEARCH PRODUCT
Anti-proliferative effect of main dietary phytosterols and β-cryptoxanthin alone or combined in human colon cancer Caco-2 cells through cytosolic Ca+2 – and oxidative stress induced apoptosis
Antonio CillaReyes BarberáM. A. LivreaAlessandro AttanzioLuisa Tesorieresubject
PopulationMedicine (miscellaneous)ApoptosisPharmacologymedicine.disease_causeβ-Cryptoxanthinchemistry.chemical_compoundSettore BIO/10 - BiochimicamedicineTX341-641educationCaco-2 cellsCaspaseeducation.field_of_studyNutrition and DieteticsbiologyNutrition. Foods and food supplyCancerPhytosterolsPhosphatidylserinemedicine.diseaseAnti-proliferation Apoptosis β-Cryptoxanthin Caco-2 cells PhytosterolsBiochemistrychemistryCaco-2ApoptosisAnti-proliferationbiology.proteinIntracellularOxidative stressFood Sciencedescription
β-cryptoxanthin (β-Cx) and phytosterols (Ps) have potential against different cancer types, including colon cancer. However, their combined action has not been reported so far. Human colon cancer Caco-2 cells were treated 24 h with β-Cx and/or main dietary Ps (β-sitosterol, campesterol and stigmasterol), alone or in combination, at concentrations compatible with physiological human serum levels. A decrease in cell viability due to apoptosis (rise in sub-G1 population and exposure of membrane phosphatidylserine) was accompanied with dephosphorylation of BAD, mitochondrial depolarization and caspase 3-dependent PARP cleavage, with intracellular Ca2+ influx and increase of RONS levels as initial triggers. Ps and β-Cx, alone or in combination showed anti-proliferative activity against human colon adenocarcinoma Caco-2 cells through the mitochondrial pathway of apoptosis. No additive or synergistic effects were observed. The importance of bioactivity-guided assays with mixtures of dietary bioactive compounds to determine their eventual interactions in the functional food context is demonstrated.
year | journal | country | edition | language |
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2015-01-01 |