Thymus essential oil extraction, characterization and incorporation in phospholipid vesicles for the antioxidant/antibacterial treatment of oral cavity diseases

6533b855fe1ef96bd12b13e5

RESEARCH PRODUCT

Thymus essential oil extraction, characterization and incorporation in phospholipid vesicles for the antioxidant/antibacterial treatment of oral cavity diseases

Guy D'hallewin Alessandra Palmieri Carla Caddeo Anna Maria Fadda Iris Usach José Esteban Peris Elvira Escribano Giacomo Luigi Petretto Roberto Pinna Maria Manconi Maria Letizia Manca Mohammad Firoznezhad Egle Milia

subject

Keratinocytes 0301 basic medicine food.ingredient Oral cavity bacteria Cell Survival Surface Properties 030106 microbiology Microbial Sensitivity Tests 02 engineering and technology Lecithin Antioxidants law.invention Thymus Plant Glycols 03 medical and health sciences chemistry.chemical_compound Colloid and Surface Chemistry food law Oils Volatile Phospholipid vesicles Glycerol Humans Particle Size Physical and Theoretical Chemistry Phospholipids Essential oil Liposome biology Chemistry Vesicle Streptococcus Thymus essential oil Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Streptococcus mutans Anti-Bacterial Agents Lactobacillus Streptococcus sanguinis Biochemistry Oral mucosa Mouth Diseases 0210 nano-technology Antibacterial activity Biotechnology

description

The aim of the work was to extract, characterize, and formulate Thymus capitatus (Tymbra capitata) essential oil in phospholipid vesicles: liposomes, glycerosomes and Penetration Enhancer-containing Vesicles (PEVs). The steam-distilled essential oil was mainly composed of carvacrol. The oil was mixed with lecithin and water to produce liposomes, or different ratios of water/glycerol or water/propylene glycol (PG) to produce glycerosomes and PG-PEVs, respectively. Cryo-TEM showed the formation of unilamellar, spherical vesicles, and light scattering disclosed that their size increased in the presence of glycerol or PG, which improved long-term stability. The formulations were highly biocompatible, and capable of counteracting oxidative stress and favouring wound repair in keratinocytes, thanks to enhanced uptake. The antibacterial activity of the oil was demonstrated against cariogenic Streptococcus mutans, Lactobacillus acidophilus, and commensal Streptococcus sanguinis. The combination of antioxidant and antibacterial activities of Thymus essential oil formulations may be useful for the treatment of oral cavity diseases.

year	journal	country	edition	language
2018-01-01	Colloids and Surfaces B: Biointerfaces

https://doi.org/10.1016/j.colsurfb.2018.07.021