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RESEARCH PRODUCT
Moringa oleifera Leaf Powder as Functional Additive in Cookies to Protect SH-SY5Y Cells
Jesús Lozano SánchezRomano LapasinDomenico NuzzoPasquale Massimo PiconePier Luigi San BiagioDonatella BuloneClelia DispenzaAlessandro GuiducciIsabel Borrás-linaresE. MuscolinoDaniela Giacomazzasubject
TechnologySH-SY5YAntioxidantQH301-705.5QC1-999Bioactive moleculesmedicine.medical_treatmentCytotoxicitymedicine.disease_causeMORINGA OLEIFERA LEAFantioxidant effectsMoringachemistry.chemical_compoundHPLC-ESI-TOF-MS identificationsAntioxidant effects Cytotoxicity HPLC-ESI-TOF-MS identifications Moringa leaf powder Rheologymedicinemoringa leaf powderGeneral Materials ScienceOxidative injuryFood scienceBiology (General)QD1-999InstrumentationFluid Flow and Transfer ProcessesTPhysicsProcess Chemistry and TechnologyGeneral Engineeringfood and beveragesmoringa leaf powder; HPLC-ESI-TOF-MS identifications; cytotoxicity; antioxidant effects; rheology; SEMEngineering (General). Civil engineering (General)Moringa leaf powderComputer Science ApplicationsChemistrychemistryAntioxidant effectsSEMcytotoxicityrheologySettore CHIM/07 - Fondamenti Chimici Delle TecnologieTA1-2040KaempferolRheologyOxidative stressdescription
The aim of this work is the evaluation of the addition of Moringa leaf powder (MLP) in cookies in terms of antioxidant properties, dough processability and sensorial properties of the cookies. The total content of biophenols and flavonoids in MLP was detected and the identification of the bioactive molecules was performed by HPLC-ESI-TOF-MS measurements, before and after oven treatment at 180 ◦C for 20 min. After a preliminary evaluation of the MLP water soluble fraction (MLPsf) cytotoxicity, its protective effect against an oxidative injury induced in the SH-SY5Y cells was assessed. Data evidence that the bioactive molecules present in MLPsf are effective in preventing ROS production and in protecting neuronal cells against oxidative stress. Prototypes of cookies containing MLP in different concentrations were then produced and evaluated by a consumer panel. Selected doughs containing MLP were analysed to determine the total content of biophenols in the cookies after baking and their enrichment in terms of valuable chemical elements. The influence of MLP on the viscoelastic behaviour and morphology of the doughs was also assessed. Finally, the potential role in counteracting the insurgence of not treatable neurodegenerative pathologies of two main MLP components, glucomoringin and kaempferol derivatives, present also after the thermal treatment, was discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-10-26 |