6533b85ffe1ef96bd12c259e
RESEARCH PRODUCT
Methylthioacetaldehyde, a possible intermediate metabolite for the production of volatile sulphur compounds from L-methionine by Lactococcus lactis
Etienne SemonPascal BonnarmeFelix AmaritaMireille YvonHenry E. SpinnlerEmilie Chambellonsubject
TransaminationMetabolitechemistry.chemical_elementMethanethiolAcetaldehydeSulfidesMicrobiologyOxygenGas Chromatography-Mass Spectrometry03 medical and health scienceschemistry.chemical_compoundMethionineCheeseGeneticsOrganic chemistrySulfhydryl CompoundsMolecular Biology[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologyLACTOCCOCUS LACTISComputingMilieux_MISCELLANEOUS030304 developmental biologychemistry.chemical_classification0303 health sciencesMethioninebiology030306 microbiologyLactococcus lactisbiology.organism_classificationSulfurLactococcus lactisL-METHIONINEMetabolic pathway[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitologychemistrydescription
Volatile sulphur compounds (VSCs) production from L-methionine was studied in Lactococcus lactis. In vitro studies with radiolabelled L-methionine and resting cells of L. lactis revealed that L-methionine was initially converted to alpha-keto-gamma-methylthiobutyrate (KMBA) by a transamination reaction. A part of KMBA was subsequently chemically converted to methylthioacetaldehyde, methanethiol and dimethylsulphides. Chemical conversion of KMBA to methylthioacetaldehyde was dependent on pH, Mn(II) and oxygen. Since methanethiol and dimethylsulphide production was highly related to that of methylthioacetaldehyde, the latter compound was proposed as being an intermediate in VSCs production by L. lactis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-07-01 |