6533b860fe1ef96bd12c2f2c
RESEARCH PRODUCT
Infrared based saliva screening test for COVID‐19
Shivani PasrichaAnthony K EdenJason A. RobertsDavid Perez-guaitaDavid Perez-guaitaDavid Perez-guaitaJitraporn VongsvivutPhilip HeraudPhilip HeraudPhilip HeraudDamian F. J. PurcellJulie L. McauleySamantha L. GrimleyMark J. TobinJulian DruceSupti RoyMike CattonBayden R. WoodBayden R. WoodDale I. GodfreyDale I. GodfreyMatthew J. BakerDale ChristensenKamila KochanDiana E. BedollaDiana E. BedollaDiana E. BedollaNatalia Salazar-quirozKeith R. BamberyAaron Lawson McleanDeborah A WilliamsonDeborah A Williamsonsubject
SalivaMaterials scienceCoronavirus disease 2019 (COVID-19)Spectrophotometry InfraredInfraredPoint-of-care testingInfrared spectroscopy010402 general chemistry01 natural sciencesProof of Concept StudySensitivity and SpecificityCatalysisSpecimen HandlingCohort Studies03 medical and health sciences0302 clinical medicineCOVID-19 TestingSpectrophotometryChlorocebus aethiopsmedicineCOVID-19 diagnostic infrared spectroscopy Raman spectroscopy saliva SARS-CoV-2 virionsAnimalsHumansQDLeast-Squares AnalysisSalivaVero CellsMass screening030304 developmental biologyDetection limit0303 health sciencessalivaChromatographymedicine.diagnostic_test010405 organic chemistrySARS-CoV-2COVID-19Discriminant AnalysisGeneral Chemistry030206 dentistryGeneral Medicine0104 chemical sciences3. Good healthPoint-of-Care TestingMonte Carlo MethodATR-FTIRResearch Articledescription
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-06-29 | Angewandte Chemie (International Ed. in English) |