6533b871fe1ef96bd12d2649
RESEARCH PRODUCT
Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules.
Vesa P. HytönenJ. Jussi ToppariVeikko LinkoJenni LeppiniemiBoxuan ShenEinari A. Niskanensubject
Bridging (networking)Sensing applicationsFOS: Physical sciencesNanotechnology02 engineering and technologyDNA-Directed DNA PolymeraseCondensed Matter - Soft Condensed Matter03 medical and health sciencesMoleculeNanotechnologyGeneral Materials SciencePhysics - Biological PhysicsElectrodesPolymerase030304 developmental biologyDNA PrimersFluorescent Dyes0303 health sciencesbiologyImmobilized DNAta114OligonucleotideChemistryta1182Molecular electronicsDNA021001 nanoscience & nanotechnologyCondensed Matter - Other Condensed MatterBiological Physics (physics.bio-ph)Electrodebiology.proteinSoft Condensed Matter (cond-mat.soft)Gold0210 nano-technologyNucleic Acid Amplification TechniquesOther Condensed Matter (cond-mat.other)description
We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-01-01 | Nanoscale |