Search results for " MS"
showing 10 items of 169 documents
Forced Retroevolution of an RNA Bacteriophage
2000
AbstractThe operator hairpin ahead of the replicase gene in RNA bacteriophage MS2 contains overlapping signals for binding the coat protein and ribosomes. Coat protein binding inhibits further translation of the gene and forms the first step in capsid formation. The hairpin sequence was partially randomized to assess the importance of this structure element for the bacteriophage and to monitor alternative solutions that would evolve on the passaging of mutant phages. The evolutionary reconstruction of the operator failed in the majority of mutants. Instead, a poor imitation developed containing only some of the recognition signals for the coat protein. Three mutants were of particular inter…
A simple method to directly retrieve reference evapotranspiration from geostationary satellite images
2013
Abstract Application of FAO-56 methodology for the assessment of reference evapotranspiration, ET 0 , is challenging in areas of the world with sparse meteorological network stations. For this reason alternative procedures using remotely observed data have been proposed in the literature. In this work, a simplified version of the Makkink approach [J. Inst. Wat. Eng. 11: 277–288, 1957] was tested in a typical Mediterranean environment (Sicily, Italy). The implemented Makkink approach (MAK) uses remotely estimated solar radiation derived from Meteosat Second Generation (MSG) satellite data and in situ observations of air temperature to assess ET 0 at daily time scale. Alternatively, taking ad…
Limits on neutral Higgs boson production in the forward region in $pp$ collisions at $\sqrt{s} = 7$ TeV
2013
Limits on the cross-section times branching fraction for neutral Higgs bosons, produced in p p collisions at root s = 7 TeV, and decaying to two tau leptons with pseudorapidities between 2.0 and 4.5, are presented. The result is based on a dataset, corresponding to an integrated luminosity of 1.0 fb(-1), collected with the LHCb detector. Candidates are identified by reconstructing final states with two muons, a muon and an electron, a muon and a hadron, or an electron and a hadron. A model independent upper limit at the 95% confidence level is set on a neutral Higgs boson cross-section times branching fraction. It varies from 8.6 pb for a Higgs boson mass of 90 GeV to 0.7 pb for a Higgs bos…
(Supplement Table S2) Results of 230Th/U-dating
2016
We compared the suitability of two skeletal materials of the Atlantic brain coral Diploria strigosa for 230Th/U-dating: the commonly used bulk material comprising all skeletal elements and the denser theca wall material. Eight fossil corals of presumably Last Interglacial age from Bonaire, southern Caribbean Sea, were investigated, and several sub-samples were dated from each coral. For four corals, both the ages and the activity ratios of the bulk material and theca wall agree within uncertainty. Three corals show significantly older ages for their bulk material than for their theca wall material as well as substantially elevated 232Th content and (230Th/238U) ratios. The bulk material sam…
Occurrence of glyphosate in beer from the Latvian market.
2018
A sensitive LC-MS/MS method for the determination of glyphosate in beer has been developed, validated, and applied to analyse 100 beer samples from 24 different producers and distributors in Latvia. The selected samples represented most beer brands and varieties sold in local supermarkets. Different procedures for sample preparation and chromatographic separation were compared. The final version of the method consisted of solid phase extraction, chromatographic separation on aminopropyl stationary phase, and detection using tandem mass spectrometry. The concentration of glyphosate in beer varied from below the LOD of 0.2 μg kg-1 up to 150 μg kg-1, higher than previously reported. Significan…
Analysis of cannabinoids by liquid chromatography–mass spectrometry in milk, liver and hemp seed to ensure food safety
2017
A method for determining cannabinoids, Δ9-tetrahidrocannabinol (THC), 11-nor-9-carboxy-Δ9-THC (THC-COOH) and 11-hidroxy-Δ9-THC (THC-OH) in milk, liver and hemp seeds based on liquid chromatography tandem mass spectrometry has been optimized and validated. Analytes were extracted with methanol and the extracts cleaned-up by solid-phase extraction using Oasis HLB (60 mg). The developed method was validated according to the Commission Decision 2002/657/EC. The decision limit (CCα) and detection capability (CCβ) ranged from 3.10–10.5 ng g−1 and 3.52–11.5 ng g−1, the recoveries were 76–118% and matrix effect ranged from −17.8% to 19.9% in the three matrices studied. The method was applied to foo…
A new multi analytical approach for the identification of synthetic and natural dyes mixtures. The case of orcein-mauveine mixture in a historical dr…
2017
In this paper, the application of a multi-analytical approach for the characterisation of synthetic and natural dyes in a historical textile is presented. The work is focused on a historical dress of a Sicilian noblewoman, dating from about 1865–1870. Firstly, SERS on fibre was performed, in order to individuate the classes of dyes employed. The SERS spectra suggested the presence of two main dyes: mauveine and orcein. In order to confirm these preliminary results, two different extraction protocols were applied. The extracts obtained were analysed by ESI-MS, MALDI-ToF and UHPCL-MS analyses, confirming the SERS results. In particular, the application of the ammonia mild extraction technique…
Skābeklis-18 bagātinātā ūdens sastāvs pēc apstarošanas, tā attīrīšana un iespējamā izmantošana atkārtoti
2017
18O bagātinātā ūdens sastāvs pēc apstarošanas, tā attīrīšana un iespējamā izmantošana atkārtoti. Andersons I., darba vadītāji Prof. Dr.h.c. Chem Andris Actiņš., zinātniskie konsultanti: SIA ’’Nukleārās medicīnas centrs’’ Ražošanas departamenta direktors Mg. Chem Jāzeps Malnačs, SIA ’’Nukleārās medicīnas centrs’’ Galvenais inženieris Dipl Ing. Linda Meistere. Maģistra darbs, 34 lappuses, 7 attēli, 6 tabula, 22 literatūras avoti, 8 pielikumi. Latviešu valodā. Darbā ir apkopota literatūra par 18O bagātinātā ūdens iegūšanu, par tā atdalīšanu no 18F izotopa izmantojot cietfāzes ekstrakciju, par bagātinātā ūdens attīrīšanas metodēm un iespējamo piemaisījumu noteikšanas metodēm. Praktisko eksperim…
NanoSr - A New Carbonate Microanalytical Reference Material for In Situ Strontium Isotope Analysis
2019
The in situ measurement of Sr isotopes in carbonates by MC‐ICP‐MS is limited by the availability of suitable microanalytical reference materials (RMs), which match the samples of interest. Whereas several well‐characterised carbonate reference materials for Sr mass fractions > 1000 µg g−1 are available, there is a lack of well‐characterised carbonate microanalytical RMs with lower Sr mass fractions. Here, we present a new synthetic carbonate nanopowder RM with a Sr mass fraction of ca. 500 µg g−1 suitable for microanalytical Sr isotope research (‘NanoSr’). NanoSr was analysed by both solution‐based and in situ techniques. Element mass fractions were determined using EPMA (Ca mass fraction),…
Degrees of freedom effect on fragmentation in tandem mass spectrometry of singly charged supramolecular aggregates of sodium sulfonates
2012
The characteristic collision energy (CCE) to obtain 50% fragmentation of positively and negatively single charged noncovalent clusters has been measured. CCE was found to increase linearly with the degrees of freedom (DoF) of the precursor ion, analogously to that observed for synthetic polymers. This suggests that fragmentation behavior (e.g. energy randomization) in covalent molecules and clusters are similar. Analysis of the slope of CCE with molecular size (DoF) indicates that activation energy of fragmentation of these clusters (loss of a monomer unit) is similar to that of the lowest energy fragmentation of protonated leucine-enkephalin. Positively and negatively charged aggregates be…