Search results for " Research Design"

showing 10 items of 95 documents

Acute Hormonal and Force Responses to Combined Strength and Endurance Loadings in Men and Women: The “Order Effect”

2013

Purpose To examine acute responses and recovery of serum hormones and muscle force following combined strength (S) and endurance (E) loading sessions in which the order of exercises is reversed (ES vs. SE). Methods This cross-over study design included recreationally endurance trained men and women (age 21–45 years, n = 12 men n = 10 women) who performed both loadings. Maximal bilateral isometric strength (MVC), isometric rate of force development (RFD) and serum concentrations of testosterone (T), cortisol (C), growth hormone (GH), insulin-like growth factor 1 (IGF-1), binding protein 3 (IGFBP3) and sex hormone binding globulin (SHBG) were measured during and after both loadings. Results B…

AdultMalemedicine.medical_specialtyendocrineAnatomy and PhysiologyStrength trainingClinical Research DesignIGFBP3lcsh:MedicineEndocrine SystemIsometric exerciseYoung AdultSex hormone-binding globulinEndocrinologyInternal medicinemedicineHumansMuscle StrengthSports and Exercise Medicinelcsh:ScienceBiologyMusculoskeletal SystemTestosteroneHydrocortisoneMultidisciplinarySurvey ResearchCross-Over StudiesbiologyEndocrine Physiologybusiness.industrylcsh:Rconcurrent strength and endurance trainingMiddle AgedCrossover studyhormonitHormonesEndocrinologyCross-Sectional Studiesbiology.proteinBody CompositionPhysical EnduranceMedicinelcsh:QFemaleneuromuscularbusinessHormonemedicine.drugResearch ArticlePLoS ONE
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Transition Care in Anorexia Nervosa Through Guidance Online from Peer and Carer Expertise (TRIANGLE): Study Protocol for a Randomised Controlled Trial

2017

TRIANGLE is a multicentre trial investigating whether the addition of a novel intervention for patients and carers (ECHOMANTRA) to treatment as usual (TAU) improves outcomes for people with anorexia nervosa (AN). ECHOMANTRA is based on the cognitive interpersonal model of AN and includes assessments, workbooks, videos, online groups and joint Skype sessions for patients and carers. People receiving intensive hospital treatment (N = 380) will be randomised to TAU or TAU plus ECHOMANTRA. Participants will be assessed over an 18-month period following randomisation. The primary outcome is patient psychological well-being at 12 months postrandomisation. Secondary outcomes include (i) patient's …

Adultanorexia nervosa carer skills guided self-help skills sharing Adult Anorexia Nervosa Caregivers Follow-Up Studies Humans Internet Peer Group Quality of Life Research Design Transitional Care Treatment OutcomeInternetanorexia nervosa; carer skills; eating disorders; guided self-help; skills sharingTransitional Careeating disorderscarer skillsPeer Groupguided self-helpanorexia nervosaHTreatment OutcomeCaregiversResearch DesignSettore M-PSI/08 - Psicologia ClinicaQuality of LifeHumansskills sharingFollow-Up Studies
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Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.

2014

[Objectives] The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes. [Design] A case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and …

Bacterial DiseasesMaleBiopsyGingivaDentistryGene ExpressionPilot ProjectsEpitheliumMonocytesGingivitisGenotypehealth care economics and organizationsPlasma cellsMultidisciplinaryGingival AbscessesbiologyQRMiddle AgedGingivitishumanitiesmedicine.anatomical_structureInfectious DiseasesCOX-2 6681231 genotype interleukin-6 periodontitisCytokinesPeriodontal AbscessesMedicineFemalemedicine.symptomPeriodontal IndexConnective tissueImmunohistochemical AnalysisResearch ArticleAdultmedicine.medical_specialtyClinical Research DesignScienceOral MedicineConnective tissueHemorrhagePolymorphism Single NucleotideInternal medicinemedicineGeneticsHumansInterleukin 6PeriodontitisBiologyAgedPeriodontitisClinical GeneticsInflammationbusiness.industryInterleukin-6Case-control studymedicine.diseaseChronic periodontitisHaplotypesCyclooxygenase 2Immune SystemCase-Control StudiesChronic Periodontitisbiology.proteinGenetic PolymorphismImmunologic TechniquesClinical ImmunologybusinessPopulation GeneticsPLoS ONE
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Assessing autophagy in archived tissue or how to capture autophagic flux from a tissue snapshot

2020

This article belongs to the Special Issue Autophagy in Cancer.

Bioquímicaautophagy:Ciências da Saúde [Ciências Médicas]Ciências Médicas::Ciências da SaúdeCellular homeostasisAutofagia610 Medicine & healthBiologyGeneral Biochemistry Genetics and Molecular Biology:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]03 medical and health sciences0302 clinical medicineHuman disease:Chemicals and Drugs::Biological Factors::Biological Markers [Medical Subject Headings]:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Autophagy [Medical Subject Headings]lcsh:QH301-705.5030304 developmental biology0303 health sciencesdiseaseBiología molecularScience & TechnologyGeneral Immunology and MicrobiologyMechanism (biology)CommunicationAutophagyautophagy; biomarkers; pathology; diseasebiomarkersPatología3. Good healthCell biology:Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Epidemiologic Research Design::Reproducibility of Results [Medical Subject Headings]BiomarcadoresTejidoslcsh:Biology (General)030220 oncology & carcinogenesis570 Life sciences; biologypathologyGeneral Agricultural and Biological SciencesFlux (metabolism)Enfermedad:Phenomena and Processes::Physiological Phenomena::Physiological Processes::Homeostasis [Medical Subject Headings]
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Oro-gustatory perception of dietary lipids and calcium signaling in taste bud cells are altered in nutritionally obesity-prone Psammomys obesus.

2013

Since the increasing prevalence of obesity is one of the major health problems of the modern era, understanding the mechanisms of oro-gustatory detection of dietary fat is critical for the prevention and treatment of obesity. We have conducted the present study on Psammomys obesus, the rodent desert gerbil which is a unique polygenic natural animal model of obesity. Our results show that obese animals exhibit a strong preference for lipid solutions in a two-bottle test. Interestingly, the expression of CD36, a lipido-receptor, in taste buds cells (TBC), isolated from circumvallate papillae, was decreased at mRNA level, but remained unaltered at protein level, in obese animals. We further st…

CD36 AntigensMaleTasteAnatomy and PhysiologyCD36BiochemistryCalcium in biologyFatschemistry.chemical_compoundMolecular Cell BiologySignaling in Cellular ProcessesMembrane Receptor Signalingchemistry.chemical_classificationMultidisciplinarybiologyQRTaste PerceptionTaste BudsLipidsSensory SystemsLipid SignalingCytochemistryThapsigarginMedicinePsammomysDisease SusceptibilityIntracellularResearch ArticleSignal Transductionmedicine.medical_specialtyThapsigarginClinical Research DesignLinoleic acidScienceLinoleic AcidFood PreferencesInternal medicinemedicineAnimalsCalcium SignalingObesityAnimal Models of DiseaseBiologyNutritionCell MembraneFatty acidProteinsbiology.organism_classificationLipid MetabolismDietary FatsGustatory SystemTransmembrane ProteinsEndocrinologyMetabolismchemistryGene Expression Regulationbiology.proteinGerbillinaeMembrane CompositionNeurosciencePLoS ONE
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Specific recommendations for PROs and HRQoL assessment in allergic rhinitis and/or asthma: a GA(2)LEN taskforce position paper

2010

The GA(2)LEN taskforce on Patient-Reported Outcomes (PROs) and Health-Related Quality of Life (HRQoL) published in 2009 a position paper concerning PROS and HRQoL assessment in clinical trials on allergy. Because of the specificity of this topic in asthma and rhinitis, specific recommendations are needed. The aim of this position paper is to define PROs and their meaning in asthma and rhinitis research, explore the available tools to provide criteria for a proper choice, identify patient-related factor which could influence PROs assessment, define specific recommendations for assessment, analysis and results spreading, underline the unexplored areas and unmet needs. PROs assessment is gaini…

Clinical Trials as TopicRhinitis Allergic PerennialHealth Planning GuidelinesRhinitis Allergic SeasonalAsthmahumanitiesOutcome Assessment (Health Care)Treatment OutcomeResearch DesignRhinitis Allergic Perennial; Humans; Rhinitis Allergic Seasonal; Health Planning Guidelines; Treatment Outcome; Clinical Trials as Topic; Quality of Life; Asthma; Outcome Assessment (Health Care); Research DesignHealth Planning GuidelineOutcome Assessment Health CareQuality of LifeHumansRinite Alérgica SazonalAsmaRhinitiQualidade de VidaHuman
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The Global Research Collaboration of Network Meta-Analysis: A Social Network Analysis

2016

et al.

Comparative Effectiveness ResearchSocial Scienceslcsh:MedicineCochrane LibraryGeographical locationsMathematical and Statistical Techniques0302 clinical medicineSociologyMedicine and Health SciencesCentrality030212 general & internal medicineHealth Systems Strengtheninglcsh:ScienceSocial network analysisMultidisciplinaryResearch Assessment3. Good healthProfessionsSystematic reviewSocial NetworksResearch Design030220 oncology & carcinogenesisMeta-analysisPhysical SciencesNetwork AnalysisStatistics (Mathematics)Research ArticleComputer and Information SciencesCanadaSystematic ReviewsClinical Research DesignMEDLINELibrary scienceResearch and Analysis Methods03 medical and health sciencesPolitical scienceStatistical MethodsChinaProductivityHealth Care Policylcsh:RHealth CareRankingPeople and PlacesNorth AmericaScientistsPopulation Groupingslcsh:QMathematicsMeta-AnalysisPLOS ONE
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The pharmacological and non-pharmacological treatment of attention deficit hyperactivity disorder in children and adolescents: A systematic review wi…

2017

Background Attention deficit hyperactivity disorder (ADHD) is one of the most commonly diagnosed psychiatric disorders in childhood. A wide variety of treatments have been used for the management of ADHD. We aimed to compare the efficacy and safety of pharmacological, psychological and complementary and alternative medicine interventions for the treatment of ADHD in children and adolescents. Methods and findings We performed a systematic review with network meta-analyses. Randomised controlled trials (≥ 3 weeks follow-up) were identified from published and unpublished sources through searches in PubMed and the Cochrane Library (up to April 7, 2016). Interventions of interest were pharmacolo…

Complementary TherapiesMaleTrastorns de l'atencióPoison controllcsh:MedicineMathematical and Statistical Techniques0302 clinical medicineBehavior TherapyMedicine and Health SciencesMedicine030212 general & internal medicineChildlcsh:ScienceRandomized Controlled Trials as TopicMultidisciplinaryPharmaceuticsMethylphenidate3. Good healthGuanfacineAntidepressant Drug TherapyNeurologyTolerabilityBehavioral PharmacologyResearch DesignPhysical SciencesFemaleStatistics (Mathematics)Research Articlemedicine.drugNeurological Drug Therapymedicine.medical_specialtyAdolescentClinical Research DesignNeuropsychiatric DisordersResearch and Analysis MethodsPlacebo03 medical and health sciencesDevelopmental NeuroscienceDrug TherapyInternal medicineMental Health and PsychiatryHumansAttention deficit hyperactivity disorderPsiquiatriaStatistical MethodsAdverse effectPsychiatryPharmacologyBehaviorbusiness.industryAtomoxetinelcsh:RCentral Nervous System DepressantsBiology and Life Sciencesmedicine.diseaseAttention Deficit Disorder with HyperactivityNeurodevelopmental DisordersCentral Nervous System StimulantsAdhdlcsh:QAdverse EventsbusinessMental Health TherapiesMathematics030217 neurology & neurosurgeryNeuroscienceMeta-AnalysisPLoS ONE
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Rationale, design and methods of CTCA-PRORECAD (Computed Tomography Coronary Angiography Prognostic Registry for Coronary Artery Disease): a multicen…

2013

PURPOSE: This study was done to assess the prognostic value of computed tomography coronary angiography (CTCA) in a large multicentre population of patients with suspected coronary artery disease (CAD) and, in particular, its incremental value compared with traditional methods for risk stratification. MATERIALS AND METHODS: This is a retrospective observational study that began in January 2003 conducted on patients with suspected CAD assessed with CTCA on the basis of symptoms (chest pain, dyspnoea) and/or abnormal or equivocal stress test and/or a high cardiovascular risk profile. The participating centres will provide data obtained with CTCA performed with 16-slice or higher equipment. Ex…

Coronary angiographyMaleContrast MediaComputed tomographyCoronary DiseaseCoronary AngiographyCoronary artery diseaseComputed tomography coronary angiographyCoronary artery diseaseComputer-AssistedRisk FactorsRegistriesTomographyNeuroradiologyeducation.field_of_studymedicine.diagnostic_testcomputed tomography coronary angiography; coronary artery disease; prevalence of disease; prognosis; registry; risk stratificationRadiographic Image InterpretationInterventional radiologyGeneral MedicineMiddle AgedPrognosisX-Ray ComputedResearch DesignRisk stratificationCardiologyPrevalence of diseaseRadiographic Image Interpretation Computer-AssistedFemaleRadiologymedicine.medical_specialtyRegistryPrognosiEndpoint DeterminationPopulationRisk AssessmentComputed tomography coronary angiography; Coronary artery disease; Prevalence of disease; Prognosis; Risk stratification; Registry; Analysis of Variance; Contrast Media; Coronary Disease; Endpoint Determination; Female; Humans; Male; Middle Aged; Prognosis; Proportional Hazards Models; Radiographic Image Interpretation Computer-Assisted; Retrospective Studies; Risk Assessment; Risk Factors; Coronary Angiography; Registries; Research Design; Tomography X-Ray ComputedInternal medicinemedicineHumansRadiology Nuclear Medicine and imagingeducationRisk stratificationProportional Hazards ModelsRetrospective StudiesAnalysis of Variancebusiness.industrymedicine.diseasebusinessTomography X-Ray ComputedLa Radiologia medica
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A Xenogeneic-Free Protocol for Isolation and Expansion of Human Adipose Stem Cells for Clinical Uses

2013

Human adipose stem cells (hASCs) play a crucial role in the fields of regenerative medicine and tissue engineering for different reasons: the abundance of adipose tissue, their easy harvesting, the ability to multipotent differentiation and the fact that they do not trigger allogeneic blood response or secrete cytokines that act as immunosuppressants. The vast majority of protocols use animal origin reagents, with the underlying risk of transmitting infections by non-human pathogens. We have designed a protocol to isolate and maintain the properties of hASCs avoiding xenogeneic reagents. These changes not only preserve hASCs morphology, but also increase cell proliferation and maintain thei…

Cèl·lules mare neuralsCellular differentiationCell- and Tissue-Based TherapyAdipose tissueCell SeparationStem cell markerRegenerative MedicineRegenerative medicine0302 clinical medicineTissue engineeringMolecular Cell BiologyAdipocytesNeurociènciesGene Regulatory Networks0303 health sciencesMultidisciplinaryStem CellsQRGene Expression Regulation DevelopmentalCell Differentiation3. Good healthCell biologyAdult Stem Cells317 Pharmacy030220 oncology & carcinogenesisMedicineStem cellCellular TypesMetabolic Networks and PathwaysResearch ArticleBiotechnologyAdultAdolescentClinical Research DesignScienceCell PotencyPrimary Cell CultureBiologyCell Growth03 medical and health sciencesYoung AdultAnimalsHumansBiology030304 developmental biologyCell ProliferationTissue EngineeringGene Expression ProfilingMesenchymal stem cellCell cultureImmunologyBiomarkersDevelopmental BiologyPLoS ONE
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