Search results for "CANDIDA"

Molecular characterization of Italian Candida parapsilosis isolates reveals the cryptic presence of the newly described species Candida orthopsilosis…

2012

The authors report the molecular characterization of Candida parapsilosis isolates recovered from the blood and venous central catheter tips of patients admitted to different care units of the Polyclinic Hospital, University of Messina, Italy. Among 97 presumed C. parapsilosis isolates examined, 94 were identified as C. parapsilosis sensu stricto and the remaining 3 isolates were found to belong to the cryptic species Candida orthopsilosis which was recovered only from blood cultures of neonates (<30 days old) born prematurely. No C. metapsilosis was found in this study. This study emphasizes the role of C. parapsilosis as an important nosocomial pathogen, and it also describes, for the fir…

Molecular evidence for ongoing complementarity and horizontal gene transfer in endosymbiotic systems of mealybugs

2014

[EN] Intracellular bacterial supply of essential amino acids is common among sap-feeding insects, thus complementing the scarcity of nitrogenous compounds in plant phloem. This is also the role of the two mealybug endosymbiotic systems whose genomes have been sequenced. In the nested endosymbiotic system from Planococcus citri (Pseudococcinae), “Candidatus Tremblaya princeps” and “Candidatus Moranella endobia” cooperate to synthesize essential amino acids, while in Phenacoccus avenae (Phenacoccinae) this function is performed by its single endosymbiont “Candidatus Tremblaya phenacola.” However, little is known regarding the evolution of essential amino acid supplementation strategies in oth…

TLR2: for or against Candida albicans?

2005

In a recent issue of Trends in Microbiology, Netea and coworkers presented their opinion that toll-like receptors (TLRs) are involved in escape from the defense mechanisms of the host [1]. In their article, the authors clearly identified three major TLR-mediated escape mechanisms that are used by microbial pathogens, such as Yersinia, Mycobacterium and Candida. Here, we wish to comment on the roll of TLR2 in Candida albicans infections. Netea's interesting hypothesis, that TLR2 expression might confer to mice an increased susceptibility to C.

Microsatellite-based genotyping of Candida parapsilosis sensu stricto isolates reveals dominance and persistence of a particular epidemiological clon…

2012

In this study, using multilocus microsatellite analysis, we report the genetic characterization of 27 Candida parapsilosis isolates recovered in two different periods of time (2007-2009 and 2011-2012) from infants hospitalized in the neonatal intensive care unit of a hospital in Messina, Italy. The results revealed the persistence and dominance of a particular infectious genotype among NICU patients and highlight the power of the used microsatellite markers in clarifying epidemiologic associations, detect micro-evolutionary variations and facilitating the recognition of outbreaks. © 2012 Elsevier B.V.

Candidemia recurrente y aislamiento de Candida auris resistente a equinocandinas en paciente portador de acceso venoso central de larga duración

2022

About the role of TLR2 and TLR4 in cytokine secretion by murine macrophages in response to Candida albicans.

2006

Dear Editor, In a recent issue of FEMS Immunology and Medical Microbiology , Blasi (2005) studied the biological role of Toll-like receptor (TLR)2 and TLR4 in the effector and secretory responses of murine macrophages to the fungal species Candida albicans . In their article, the authors conclude that the secretory response to C. albicans appears to be TLR4- but not TLR2-dependent. In our opinion this statement is misleading as the results reported do not support this conclusion and, therefore, we wish to comment on this issue. There is evidence indicating that TLR2 is the main receptor involved in triggering cytokine production by murine macrophages in response to C. albicans . Phospholipo…

Aislamiento y caracterización de ABG1, un gen esencial del hongo patógeno oportunista Candida Albicans.

2005

Mediante el inmunorrastreo de una genoteca de expresión de Candida albicans con un anticuerpo policlonal específico de la forma micelial de dicho hongo (PAb anti-gt), se ha aislado un nuevo gen de este microorganismo. La represión del gen aislado ocasionó un severo defecto en la morfogénesis de C. albicans, caracterizado por la formación de cadenas de levaduras, de tamaño decreciente en dirección al extremo apical de la cadena, por lo que el gen fue denominado ABG1 (del inglés, "altered budding growth"), en base al fenotipo de crecimiento por gemación alterado observado en las cepas mutantes para dicho gen. La secuencia de aminoácidos de Abg1p, el producto del gen ABG1, presentó los porcent…

Recurrent Mutations in the Basic Domain of TWIST2 Cause Ablepharon Macrostomia and Barber-Say Syndromes

2015

Contains fulltext : 153827.pdf (Publisher’s version ) (Open Access) Ablepharon macrostomia syndrome (AMS) and Barber-Say syndrome (BSS) are rare congenital ectodermal dysplasias characterized by similar clinical features. To establish the genetic basis of AMS and BSS, we performed extensive clinical phenotyping, whole exome and candidate gene sequencing, and functional validations. We identified a recurrent de novo mutation in TWIST2 in seven independent AMS-affected families, as well as another recurrent de novo mutation affecting the same amino acid in ten independent BSS-affected families. Moreover, a genotype-phenotype correlation was observed, because the two syndromes differed based s…

Characterization of aCandida albicansgene encoding a putative transcriptional factor required for cell wall integrity

2003

After screening a Candida albicans genome database the product of an open reading frame (ORF) (CA2880) with 49% homology to the product of Saccharomyces cerevisiae YPL133c, a putative transcriptional factor, was identified. The disruption of the C. albicans gene leads to a major sensitivity to calcofluor white and Congo red, a minor sensitivity to sodium dodecyl sulfate, a major resistance to zymolyase, and an alteration of the chemical composition of the cell wall. For these reasons we called it CaCWT1 (for C. albicans cell wall transcription factor). CaCwt1p contains a putative Zn(II) Cys(6) DNA binding domain characteristic of some transcriptional factors and a PAS domain. The CaCWT1 gen…

The 5' Untranslated Region of the

2018

Many of the virulence traits that make Candida albicans an important human fungal pathogen are regulated on a transcriptional level. Here, we report an important regulatory contribution of translation, which is exerted by the extensive 5′ untranslated regulatory sequence (5′ UTR) of the transcript for the protein Efg1, which determines growth, metabolism, and filamentation in the fungus. The presence of the 5′ UTR is required for efficient translation of Efg1, to promote filamentation. Because transcripts for many relevant regulators contain extensive 5′ UTR sequences, it appears that the virulence of C. albicans depends on the combination of transcriptional and translational regulatory mec…