Search results for "Cereal"

Optimization of Matrix Solid-Phase Dispersion method for simultaneous extraction of aflatoxins and OTA in cereals and its application to commercial s…

2010

Abstract A method based on Matrix Solid-Phase Dispersion (MSPD) has been developed for the determination of 5 mycotoxins (ochratoxin A and aflatoxins B and G) in different cereals. Several dispersants, eluents and ratios were tested during the optimization of the process in order to obtain the best results. Finally, samples were blended with C 18 and the mycotoxins were extracted with acetonitrile. Regarding to matrix effects, the results clearly demonstrated the necessity to use a matrix-matched calibration to validate the method. Analyses were performed by liquid chromatography–triple quadrupole-tandem mass spectrometry (LC–QqQ-MS/MS). The recoveries of the extraction process ranged from …

Determination of inorganic species of Sb and Te in cereals by hydride generation atomic fluorescence spectrometry

2011

A non-chromatographic fast, sensitive and easy method has been developed for the determination of Sb(III), Sb(V), Te(IV) and Te(VI) in cereal samples. The procedure is based on ultrasound assisted extraction and determination by hydride generation atomic fluorescence spectrometry (HG AFS). Preliminary studies were made in order to get the best extraction efficiency using 1 mol L-1 phosphoric acid, 1 mol L-1 nitric acid, aqua regia, 1 mol L-1 sulfuric acid and 6 mol L-1 hydrochloric acid. The extraction with aquaregia showed a clear interconversion of the species during the process, being H2SO4 the best extractant with efficiencies greater than 90% from the total content of Sb and Te quantif…

Analysis of enniatins and beauvericin by LC-MS/MS in wheat-based products

2017

Due to the matrix complexity for wheat-based products, a comparative study of different rapid extraction procedures was performed for the extraction of enniatins (ENA, ENA1, ENB, ENB1) and beauvericin in flour, pasta, breakfast cereals, and biscuits. Three different approaches were studied during the extraction and purification steps (shaker, Ultra-Turrax, and QuEChERS) for each matrix. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with electrospray source working in a positive mode was used. For the analysis of the five mycotoxins, the three methods were tested in terms of recovery, matrix effect, and sensibility, concluding that Ultra-Turrax extraction was the most …

No till soil organic carbon sequestration could be overestimated when slope effect is not considered

2020

Abstract No tillage (NT) soil management has been considered a strategy for the implementation of environmental sustainability and a possible tool of soil organic carbon (SOC) sequestration. Considering the wide range of data on SOC change after NT application in relation to conventional tillage (CT) in different studies, further researches are needed over a diverse range of soil and climate before a proper estimation of the benefits can be provided by the NT. A data set composed of cereal cropping system studies, comparing the SOC content under CT and NT was compiled from the literature using the scientific repositories “Scopus” and “Science direct”. This aims to i) discriminate and quanti…

Control de micotoxines en cereals i derivats per cromatografia liquida espectrometria de masses : avaluació de les ingestes

2012

Mycotoxins are secondary metabolites produced by few fungal species that readily colonize crops and contaminate them with toxins in the field or after harvest. Surveillance studies have showed that mycotoxin contamination is a world-wide problem, since it is estimated that 25% of the world's crop production and 20% of crop production within the European Union may be contaminated with these contaminants. Economic losses deriving from that are tremendous, including reduction of livestock production and agricultural production, health care, veterinary and regulatory costs. The methods described in this Thesis for the extraction of mycotoxins from cereal and derivates used conventional techniqu…

ASPETTI DELLA FILIERA CEREALICOLA IN SICILIA E ABITUDINI DI CONSUMO DEI PRODOTTI DERIVATI DEL GRANO DURO E DI ALTRI CEREALI IN ETÀ PREADOLESCENZIALE

2014

The Cereal Market and the Role of Fermentation in Cereal-Based Food Production in Africa

2014

Cereals represent the main crop in Africa (45% of arable land) and make an important contribution to the human diet in this continent. Cereals for humans are not consumed raw. Fermentation technology is widely used in Africa to transform cereals into edible products as well as to preserve and enhance the nutritional and safety aspects of cereals. In general, in Africa, this process is not controlled and is devoid of good manufacturing practices (GMPs). Spoilage and/or pathogenic microorganisms may compromise the final quality when the fermentation is not controlled. This article focuses on the role that the fermentation process could play in creating safe food conditions in Africa. Taking i…

Spatial Distribution of Fungal Communities in an Arable Soil.

2015

Fungi are prominent drivers of ecological processes in soils, so that fungal communities across different soil ecosystems have been well investigated. However, for arable soils taxonomically resolved fine-scale studies including vertical itemization of fungal communities are still missing. Here, we combined a cloning/Sanger sequencing approach of the ITS/LSU region as marker for general fungi and of the partial SSU region for arbuscular mycorrhizal fungi (AMF) to characterize the microbiome in different maize soil habitats. Four compartments were analyzed over two annual cycles 2009 and 2010: a) ploughed soil in 0-10 cm, b) rooted soil in 40-50 cm, c) root-free soil in 60-70 cm soil depth a…

Neural network models for prediction of trichothecene content in wheat

2008

Fusarium graminearum is a mould that causes serious diseases in cereals worldwide and that synthesises mycotoxins such as deoxynivalenol (DON), which can seriously affect human and animal health. Predicting the level of mycotoxin accumulation in food is very difficult, because of the complexity of the influencing parameters. In this work, we have studied the possibility of using artificial neural networks (NN) to predict DON level attained in F. graminearum wheat cultures taking as inputs the fungal contamination level of the cereal, the water activity as a measure of the available water for fungal growth in the cereal, the temperature and time. DON analysis was performed by gas chromatogr…

Presence of Enniatins and Beauvericin in Romanian Wheat Samples: From Raw Material to Products for Direct Human Consumption

2017

In this study, a total of 244 wheat and wheat-based products collected from Romania were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) in order to evaluate the presence of four enniatins (ENs; i.e., ENA, ENA1, ENB, and ENB1) and beauvericin (BEA). For the wheat samples, the influence of agricultural practices was assessed, whereas the results for the wheat-based products were used to calculate the estimated daily intake of emerging mycotoxins through wheat consumption for the Romanian population. ENB presented the highest incidence (41% in wheat and 32% in wheat-based products), with its maximum levels of 815 μg kg−1 and 170 μg kg−1 in wheat and wheat-based products,…