Search results for "Embryonic Structure"
showing 10 items of 624 documents
Additional file 1: of RIP-Chip analysis supports different roles for AGO2 and GW182 proteins in recruiting and processing microRNA targets
2019
Analysis of miRNA expression in AGO2 and GW182-IP samples. a) miRNA expression level in AGO2-IP samples (average value from the three performed experiments) vs the expression level in IN samples (average value from the three performed experiments). The Pearson correlation values reported on the top of the picture were computed by using all the expressed miRNA, and the top 100 or 50 expressed miRNAs. The colored points refer to miRNA that have been validated by RT-PCR data. Green points refer to hsa-miR-141-3p, hsa-miR-21-5p, hsa-let-7f-5p, hsa-miR-16-5p, hsa-miR-24-3p, hsa-miR-27a-3p, hsa-miR-23a-3p. The red point refers to hsa-miR-1260a. b) Comparison of IP/IN ratios obtained by RT-PCR dat…
Synthetic Inhibitors of Cell Adhesion: A Glycopeptide from E-Selectin Ligand 1 (ESL-1) with the Arabino Sialyl Lewisx Structure
2001
Particularly selective methods are required for the synthesis of arabino sialyl Lewisx glycopeptides owing to the acid-labile β-arabinopyranoside bond. It is important for the inhibition of cell adhesion that the arabino sialyl Lewisx glycopeptide 1, which contains the Gly 672 -Asp 681 sequence of the E-selectin Ligand 1 (ESL-1), binds ten times more strongly than sialyl Lewisx to E-selectin, although it is monovalent and does not contain L-fucose, which is considered essential.
The use of morphokinetics as a predictor of embryo implantation.
2011
Background Time-lapse observation presents an opportunity for optimizing embryo selection based on morphological grading as well as providing novel kinetic parameters, which may further improve accurate selection of viable embryos. The objective of this retrospective study was to identify the morphokinetic parameters specific to embryos that were capable of implanting. In order to compare a large number of embryos, with minimal variation in culture conditions, we have used an automatic embryo monitoring system. Methods Using a tri-gas IVF incubator with a built-in camera designed to automatically acquire images at defined time points, we have simultaneously monitored up to 72 individual emb…
Tenascin in denervated human muscle
1996
Tenascin is a large oligomeric glycoprotein of the extracellular matrix. Its location is limited in innervated muscle tissues. We investigated immunohistologically, using two monoclonal antibodies (mab) against Tenascin, biopsied denervated human muscle of children and adults. Tenascin was present in the interstitial space among denervated muscle fibres. Accumulation of Tenascin in denervated adult muscle tissue was frequent, accumulation in denervated muscle tissue of children was sparse and weak. The two antibodies reacted correspondingly. Tenascin was not only found in the vicinity of atrophic muscle fibres, but also close to normally sized fibres, suggesting an early stage of denervatio…
Interaction of Dimethyltin(IV)2+ Cation with Gly-Gly, Gly-His, and Some Related Ligands. A New Case of a Metal Ion Able To Promote Peptide Nitrogen D…
1999
Equilibrium (pH-metric) and spectroscopic (1H,13C, and 119Sn NMR and 119Sn Mossbauer) studies were performed to characterize the interaction of the dimethyltin(IV) cation with glycine, glycyl-glycine (Gly-Gly), imidazole-4-acetic acid, histamine, histidine, glycyl-histamine, glycyl-histidine (Gly-His), and β-alanyl-histidine (carnosine). For histamine and glycyl-histamine (having only nitrogen donor atoms) no complex formation was detected. The hydrolyzed species of the dimethyltin(IV) cation are always dominant over the complexes formed with the other ligands, except with Gly-Gly and Gly-His. For these two ligands, {COO-,N-,NH2} coordinated complexes are dominant in the neutral pH range wi…
Somatic embryogenesis and plant regeneration in a woody species: the European Spindle Tree (Euonymus europaeus L.).
1993
Somatic embryogenesis and subsequent plant regeneration of Euonymus europaeus L (European Spindle Tree) were obtained from square pieces of mature zygotic embryos with an intervening callus phase. Callus and somatic embryos were induced using a Murashige and Skoog's semi-solid basal medium supplemented with several combinations of auxins and cytokinins. The greatest number of somatic embryos was obtained with a continuous exposure to 22.8 μM indoleacetic acid and 0.046 μM kinetin. The frequency of somatic embryogenesis from zygotic embryos depends on the cold conservation time of seeds. The embryos frequently germinated on the same medium. Further development of somatic embryos into plantle…
Mit Donorzentren versehener korbförmiger Molekülhohlraum — Darstellung, Struktur, Eigenschaften
1991
Basket-shaped Molecular Cavity-Containing Donor Centres — Synthesis, Structure, Properties The macrocyclic basket-shaped molecule 2, composed of three 4-donor-substituted pyridine units, is synthesized by cyclisation of the chloromethyl compound 7 with sulfonamide 8. The X-ray structure analysis of 2 gives an impression of the shape of the molecular basket. Furthermore it demonstrates that the toluenesulfonamide residues interlink, creating dimeric units of 2 in the crystal.
Synthesis of RNA in isolated nuclei of sea urchin embryos.
1970
It is demonstrated that isolated nuclei of sea urchin embryos are able to incorporate radioactive nucleotides into RNA. Some properties of the incorporation system are described.
Glucose Diffusion Coefficients Determined from Concentration Profiles in Emt6 Tumor Spheroids Incubated in Radioactively Labeled L-Glucose
1994
In order to theoretically assess tissue energetic status, conditions for substance exchange need to be known. One group of parameters important in this context are diffusion coefficients of nutrients and metabolic waste products which may be assessed by incubating spheroids in a medium containing tracer amounts of the radioactively labeled substance in question, for a defined period of time. In previous studies, the overall amount of 14C-labeled substance taken up by the spheroids was measured by scintillation counters (e.g.[1]), or the concentration of 3H-labeled substance in the spheroid center was determined by autoradiography and grain counting (e.g. [4]). From a number of such measurem…
The hedgehog receptor patched is involved in cholesterol transport.
2011
International audience; BACKGROUND: Sonic hedgehog (Shh) signaling plays a crucial role in growth and patterning during embryonic development, and also in stem cell maintenance and tissue regeneration in adults. Aberrant Shh pathway activation is involved in the development of many tumors, and one of the most affected Shh signaling steps found in these tumors is the regulation of the signaling receptor Smoothened by the Shh receptor Patched. In the present work, we investigated Patched activity and the mechanism by which Patched inhibits Smoothened. METHODOLOGY/PRINCIPAL FINDINGS: Using the well-known Shh-responding cell line of mouse fibroblasts NIH 3T3, we first observed that enhancement …