Search results for "Fused"
showing 10 items of 50 documents
ALS-linked FUS mutations confer loss and gain of function in the nucleus by promoting excessive formation of dysfunctional paraspeckles
2019
Mutations in the FUS gene cause amyotrophic lateral sclerosis (ALS-FUS). Mutant FUS is known to confer cytoplasmic gain of function but its effects in the nucleus are less understood. FUS is an essential component of paraspeckles, subnuclear bodies assembled on a lncRNA NEAT1. Paraspeckles may play a protective role specifically in degenerating spinal motor neurons. However it is still unknown how endogenous levels of mutant FUS would affect NEAT1/paraspeckles. Using novel cell lines with the FUS gene modified by CRISPR/Cas9 and human patient fibroblasts, we found that endogenous levels of mutant FUS cause accumulation of NEAT1 isoforms and paraspeckles. However, despite only mild cytoplasm…
Iodolactonization of 3-Alkynylthiophene-2-Carboxylic and 3-Alkynylpicolinic Acids for the Synthesis of Fused Heterocycles
2020
The iodolactonization of 3-alkynylthiophene-2-carboxylic acids and 3-alkynylpicolinic acids has been investigated. Using I2 as the iodine source and NaHCO3 as the base in MeCN, the process took place smoothly to afford thienopyranones and pyranopyridinones, respectively, from 6-endo-dig cyclization. The method also worked nicely for the transformation of 2-(phenylethynyl)thiophene-3-carboxylic acid and 3-(phenylethynyl) isonicotinic acid into 7-iodo-6-phenyl-4H-thieno[3,2-c]- pyran-4-one and 4-iodo-3-phenyl-1H-pyrano[4,3-c]pyridin-1- one, respectively. Although with some 3-alkynylpicolinic acids the process led to a mixture of the 6-endo-dig and 5-exo-dig products, it could be still made se…
The application of porous silica layers in open tubular columns for liquid chromatography
1987
Two methods to realize a porous retentive silica layer on the inner wall of 10–25 µm fused silica capillaries for OTLC, etching and precipitation of silica from solution, have been investigated. Etching of the fused silica capillaries with 1M KOH, creates an activated surface, but the capacity of the silica layer is too small to serve as retentive layer in OTLC. Better prospects are offered by the precipitation of silica from a solution of polyethoxysiloxane, dynamically coated on the inner wall of the fused silica capillary. It appears to be possible to deposite a porous silica layer up to 0.8 µm thick (in a 25 µm capillary) by this method, which seems to be suitable for liquid-solid an dy…
A Straightforward Electroactive π-Extended Tetrathiafulvalene (exTTF) Building Block
2012
The synthesis and X-ray structure of a new and readily available exTTF derivative (6) bearing a methyltriphenylphosphonium bromide moiety as a new building block for the construction of electroactive molecules is reported. The phosphonium salt 6, which was prepared in one step from 2-hydroxymethyl-exTTF as a stable yellow solid in 84 % yield, efficiently undergoes Wittig olefination reactions with a variety of aldehydes to predominantly form the E isomer. Electronic spectra and cyclic voltammetry of the novel compounds reveal the electronic communication between the electroactive units.
Topological optimization of a structural naval component manufactured in FDM
2019
In this paper, a topological optimization procedure has been applied on a real component of the deck of a sailing multi-hull in order to find the internal shape that best save the material used in the manufacturing process without a relevant loss of structural rigidity. The multi-hull boat is a 16 feet length catamaran equipped with an asymmetric foil on both centerboards and with a symmetric foil on both rudders. The task of the analyzed object is to act as a cylindrical support for the screw that drives the rotation of the centerboard. The process adopted to manufacture this object is the Fused Deposition Modeling (FDM) technique, because of its high versatility and its relative low-cost …
Structure and Doping Determined Thermoelectric Properties of Bi2Se3Thin Films Deposited by Vapour–Solid Technique
2019
In this work, a simple catalyst-free vapour-solid deposition method was applied for controlled deposition of two types (planar and disordered) of continuous Bi 2 Se 3 nanostructured thin films on different (fused quartz/glass, mica, graphene) substrates. Characterisation of electron transport (type, concentration and mobility of the main charge carriers) and thermoelectric properties (Seebeck coefficient and power factor) showed that proposed in this work deposition method allows to obtain Bi 2 Se 3 thin films with power factor comparable and even higher than reported for the Bi 2 Se 3 thin films grown by molecular beam epitaxy technique. Power factor of the best obtained thin films can be …
Structure-determined thermoelectric properties of Bi2Se3 thin films deposited by vapour-solid technique
2018
International audience; In this work, a simple catalyst-free vapour-solid deposition method is applied for controlled obtaining of two types (planar and disordered) continuous Bi2Se3 nanostructured thin films on different (fused quartz/glass, mica, graphene) substrates. Performed for the deposited thin films transport and thermoelectric characterization (type, concentration and mobility of the main charge carriers, Seebeck coefficient and power factor) showed that proposed deposition method allows to fabricate “low-doped” Bi2Se3 thin films with power factor comparable and even higher than reported for the Bi2Se3 thin films fabricated by molecular beam epitaxy technique.
Radiation induced generation of non-bridging oxygen hole center in silica: Intrinsic and extrinsic processes
2007
Abstract The generation of non-bridging oxygen hole center ( Si–O ) was investigated in a wide variety of natural (fused quartz) and synthetic silica samples exposed to different γ- and β-irradiation doses by looking at its optical bands. We distinguish two different generation processes: intrinsic associated with the cleavage of Si–O bond and characterized by a sublinear law and extrinsic due to the conversion of OH precursor characterized by a growth curve with a saturating tendency. The interplay between the two processes and the role of H are discussed.
Growth of paramagnetic defects by gamma rays irradiation in oxygen-deficient silica
2005
Abstract We report an electron paramagnetic resonance (EPR) study of gamma rays irradiation effects on an oxygen-deficient silica obtained from fused quartz. We have found that three types of E′ centers, E α ′ , E γ ′ and E δ ′ , and a paramagnetic defect in a spin triplet state (S = 1) are induced. The EPR signals dependence on the microwave power have been investigated. The concentrations of these centers as a function of gamma ray irradiation show that for high dose a limit value is reached suggesting a generation process from precursors.
Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column
2015
A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. A multi-segmented gradient program using a fused-core column for the separation of several phenolic classes (phenolic acids and flavonoids) has been optimised. The influence of extraction conditions (sample freeze-drying, ultrasound extraction, solvent composition and extraction time) has been also optimised using response surface methodology with tomato samples as a model. Complete recoveries (76-108%) were obtained for the phenolic compounds present in tomato. The developed…