Search results for "Labeling"

showing 10 items of 372 documents

In vitro anti-biofilm activity of Boswellia spp. oleogum resin essential oils

2009

Aims:  To evaluate the anti-biofilm activity of the commercially available essential oils from two Boswellia species. Methods and Results:  The susceptibility of staphylococcal and Candida albicans biofilms was determined by methyltiazotetrazolium (MTT) staining. At concentrations ranging from 217·3 μg ml−1 (25% v/v) to 6·8 μg ml−1 (0·75% v/v), the essential oil of Boswellia papyrifera showed considerable activity against both Staphylococcus epidermidis DSM 3269 and Staphylococcus aureus ATCC 29213 biofilms. The anti-microbial efficacy of this oil against S. epidermidis RP62A biofilms was also tested using live/dead staining in combination with fluorescence microscopy, and we observed that …

Staphylococcus aureusTetrazolium SaltsMicrobial Sensitivity Testsmedicine.disease_causeApplied Microbiology and BiotechnologyBacterial Adhesionlaw.inventionMicrobiologylawStaphylococcus epidermidisCandida albicansmedicineOils VolatileStaphylococcus epidermidisBoswelliaCandida albicansBoswelliaBoswellia C albicans biofilms essential oils staphylococcal biofilmsEssential oilMicrobial ViabilitybiologyStaining and LabelingBiofilmbiochemical phenomena metabolism and nutritionbiology.organism_classificationCorpus albicansGrowth InhibitorsThiazolesStaphylococcus aureusBiofilmsBoswellia papyrifera
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The effect of detergents on the basement membrane complex of a biologic scaffold material

2013

The basement membrane complex (BMC) is a critical component of the extracellular matrix (ECM) that supports and facilitates the growth of cells. This study investigates four detergents commonly used in the process of tissue decellularization and their effect upon the BMC. The BMC of porcine urinary bladder was subjected to 3% Triton-X 100, 8 mM 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS), 4% sodium deoxycholate or 1% sodium dodecyl sulfate (SDS) for 24 h. The BMC structure for each treatment group was assessed by immunolabeling, scanning electron microscopy (SEM) and second harmonic generation (SHG) imaging of the fiber network. The composition was assessed by quantif…

Sus scrofaFluorescent Antibody TechniqueBiochemistryBasement MembraneGlycosaminoglycanExtracellular matrixImmunolabelingchemistry.chemical_compoundTissue ScaffoldChapsSodium dodecyl sulfateDecellularizationGlycosaminoglycansMicrovesselEndothelial CellDecellularizationTissue ScaffoldsIntegrin beta1Extracellular matrixGeneral Medicinemedicine.anatomical_structureCollagenHumanBiotechnologyDetergentMaterials scienceDetergentsBiomedical EngineeringArticleBiomaterialsImaging Three-DimensionalRe-endothelizationIn Situ Nick-End LabelingmedicineAnimalsHumansMolecular BiologyOrgan engineeringBasement membraneStaining and LabelingAnimalBiologic scaffoldAntigens CD29Endothelial CellsDNABiomaterialMolecular biologyKi-67 AntigenGlycosaminoglycanchemistryTissue DecellularizationMicrovesselsActa Biomaterialia
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Instant labeling of therapeutic cells for multimodality imaging

2020

Autologous therapeutic cells are typically harvested and transplanted in one single surgery. This makes it impossible to label them with imaging biomarkers through classical transfection techniques in a laboratory. To solve this problem, we developed a novel microfluidic device, which provides highly efficient labeling of therapeutic cells with imaging biomarkers through mechanoporation. Methods: Studies were performed with a new, custom-designed microfluidic device, which contains ridges, which compress adipose tissue-derived stem cells (ADSCs) during their device passage. Cell relaxation after compression leads to cell volume exchange for convective transfer of nanoparticles and nanoparti…

SwineCellMedicine (miscellaneous)Multimodal Imaging030218 nuclear medicine & medical imagingin vivo cell tracking03 medical and health scienceschemistry.chemical_compound0302 clinical medicineIn vivoFluorodeoxyglucose F18medicinemicrofluidic deviceAnimalsMagnetite NanoparticlesPharmacology Toxicology and Pharmaceutics (miscellaneous)mechanoporationCells Culturedmedicine.diagnostic_testStaining and LabelingChemistryStem Cellsiron oxide nanoparticlesMagnetic resonance imagingTransfectionMagnetic Resonance Imaging18F-FDGmedicine.anatomical_structureAdipose TissuePositron emission tomography030220 oncology & carcinogenesisPositron-Emission TomographyStem cellIron oxide nanoparticlesEx vivoBiomarkersBiomedical engineeringResearch PaperTheranostics
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Rapid and eco-friendly synthesis of graphene oxide-silica nanohybrids

2014

The increasing interest in Graphene oxide (GO) is due to many issues: the presence of both sp2-conjugated atoms and oxygen-containing functional groups provides a strong hydrophilicity and the possibility to further functionalize it with other molecules (i.e. π-π interactions covalent attachment etc.) [1]. Furthermore since the GO is biocompatible and noncytotoxic many studies have been recently focused on the development of GO-based nanodevices for bioimaging DNA detection drug delivery. Due to their low cytotoxicity and large internal surface area silica nanoparticles have been taken into account as promising material for biolabeling and drug loading/delivery. Particular consideration has recently been demonstrated for GO-silica composites because of the potentialities for electrical applications their chemical inertia and stability toward ions exposure. The possibility to combine the extraordinary properties of GO and silica offers several advantages for the realization of nanoprobes for biological applications and of biosensor [12]. The strategy for the fabrication of GO-nanosilica nanohybrids can be schematized as follows: (i) synthesis of GO by oxidizing graphite powder with the method described by Marcano et al. [3] (ii) Preparation of oxygen-loaded silica nanoparticles by thermal treatments in controlled atmosphere in order to induce high NIR emission at 1272 nm from high purity silica nanoparticles. (iii) preparation of GrO-silica nanohybrid films via rapid solvent casting in water. The nanohybrids were tested by XPS FTIR Raman analysis UV photoluminescence analysis TGA Zeta potential measurements electrical tests AFM and SEM. Several nanohybrids were prepared by combining two different typologies of GO and two different samples of silica.
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Electron Microscopic Contrast of the Cytoskeleton and Junctional Complexes of Intestinal Epithelial Cells by Ethanolic Phosphotungstic Acid

2000

After glutaraldehyde fixation and treatment with ethanolic phosphotungstic acid (E-PTA) before plastic embedding, sections of rat large intestine showed a characteristic electron contrasting pattern in epithelial cells. The axis of microvilli, terminal web, a thin band below the luminal plasma membrane, centrioles and junctional complexes (tight junctions, adherens junctions, and desmosomes) appeared highly contrasted. In addition to protein components of microfilaments and intermediate filaments, proteins from the junctional complexes could also be implicated in the contrasting reaction with E-PTA. Mitochondrial membranes, chromatin masses, and nucleoli of enterocytes showed considerable e…

Tissue FixationBiologyMicrofilamentSpecimen HandlingAdherens junctionTerminal webGlycocalyxchemistry.chemical_compoundAnimalsIntestine LargePhosphotungstic acidIntestinal MucosaRats WistarCytoskeletonIntermediate filamentCytoskeletonEthanolMicrovilliStaining and LabelingTissue EmbeddingTight junctionEpithelial CellsPhosphotungstic AcidAgricultural and Biological Sciences (miscellaneous)ChromatinMitochondriaRatsCell biologySolutionsMicroscopy ElectronIntercellular JunctionschemistrySolventsAnatomyCell NucleolusEuropean Journal of Morphology
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Dietary Glycaemic Index Labelling: A Global Perspective

2021

The glycaemic index (GI) is a food metric that ranks the acute impact of available (digestible) carbohydrates on blood glucose. At present, few countries regulate the inclusion of GI on food labels even though the information may assist consumers to manage blood glucose levels. Australia and New Zealand regulate GI claims as nutrition content claims and also recognize the GI Foundation’s certified Low GI trademark as an endorsement. The GI Foundation of South Africa endorses foods with low, medium and high GI symbols. In Asia, Singapore’s Healthier Choice Symbol has specific provisions for low GI claims. Low GI claims are also permitted on food labels in India. In China, there are no nation…

TrademarkHarmonizationReviewCertificationGlobal HealthBest interestsGlycaemic indexFood LabelingLabellingEnvironmental healthparasitic diseasesHumansmedia_common.cataloged_instanceTX341-641European unionmedia_commonNutrition and DieteticsdiabetesNutrition. Foods and food supplysocial sciencesDietfood regulationFood regulationGlycemic Indexglycaemic indexpopulation characteristicsBusinesshuman activitiesFood Analysisfood labelsgeographic locationsFood ScienceNutrients
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Automatic Extraction of Semantic Roles in Support Verb Constructions

2021

This paper deals with paraphrastic relations in Italian. In the following sentences: (a) Max strappò delle lacrime a Sara 'Max moved Sara to tears' and (b) Max fece piangere Sara 'Max made Sara cry', the verbs differ syntactically and semantically. Strappare 'tear/rip/wring' is transitive, fare ‘have/make’ is a causative, and piangere 'cry' is intransitive. Despite this, a translation of (a) as (b) is legitimate and therefore (a) is a paraphrase of (b). In theoretical linguistics this raises an issue concerning the relationship between strappare and fare/piangere in Italian, and that in English between move and make. In computational linguistics, can such paraphrases be obtained automatical…

Transitive relationSemantic role labelingParaphrase Entailments Meaning Extraction Automatic Detection of Semantic RolesTheoretical linguisticsVerbCausativeComputational linguisticsPsychologyParaphraseLinguisticsSettore L-LIN/01 - Glottologia E LinguisticaInternational Journal on Natural Language Computing
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Application of graph grammars in music composing systems

1987

Tree-adjoining grammarDevelopment environmentGraph rewritingGraph labelingRule-based machine translationComputer scienceProgramming languageClique-widthGraph (abstract data type)Context-sensitive grammarcomputer.software_genrecomputer
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Synthesis, X-ray Structure Determination, and Comprehensive Photochemical Characterization of (Trifluoromethyl)diazirine-Containing TRPML1 Ligands

2021

Potential (trifluoromethyl)diazirine-based TRPML1 ion channel ligands were designed and synthesized, and their structures were determined by single-crystal X-ray diffraction analysis. Photoactivation studies via 19F NMR spectroscopy and HPLC-MS analysis revealed distinct kinetical characteristics in selected solvents and favorable photochemical properties in an aqueous buffer. These photoactivatable TRPML activators represent useful and valuable tools for TRPML photoaffinity labeling combined with mass spectrometry.

TrifluoromethylPhotoaffinity labelingTRPML010405 organic chemistryX-RaysOrganic ChemistryX-rayPhotoaffinity LabelsLigands010402 general chemistryPhotochemistryMass spectrometry01 natural sciencesMass Spectrometry0104 chemical sciencesCharacterization (materials science)chemistry.chemical_compoundDiazomethanechemistry540 ChemistryDiazirine570 Life sciences; biologyIon channelThe Journal of Organic Chemistry
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The relation between tumor cell proliferation and vascularization in differentiated and undifferentiated colon carcinomas in the rat.

1982

Tumor cell proliferation and tumor vascularization were investigated in differentiated and undifferentiated colon carcinomas of the rat. The following results were found: 1, in both tumor types, vascularization is developed equally well; 2, both in the differentiated and in the undifferentiated carcinomas, the number of proliferating tumor cells decreases with increasing distance from the capillaries; at a distance of about 80 microns, there is no longer any noteworthy tumor cell proliferation; 3, the differentiated and undifferentiated carcinomas differ both in the level of the mitosis index and 3H-thymidine labeling index as well as in their local proliferation pattern despite equal vascu…

Tumor cell populationPathologymedicine.medical_specialtyLabeling indexTumor cellsNeoplasms ExperimentalBiologyTumor vascularizationmedicine.diseaseRatsColon carcinomaColonic NeoplasmsCarcinomamedicineMitotic IndexAnimalsAutoradiographyUndifferentiated carcinomaMitosisCell DivisionThymidineVirchows Archiv. B, Cell pathology including molecular pathology
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