Search results for "Malin"
showing 10 items of 110 documents
A new method to isolate lichen algae by using percoll® gradient centrifugation
2001
AbstractA rapid method to isolate intact functional algae from the lichens Evemia prunastri and Ramalina farinacea has been developed. This method is based on the use of Percoll® gradients after mechanical disruption of lichen thalli. Results obtained show that the algal preparations were virtually free of contamination by fungal hyphae. The purified algal cells were photosynthetically active and without symptoms of photoinhibition, which indicates their functional integrity. This method may be used for the isolation of intact algae from a broad range of lichen species.
Sources of geomaterials in the Sicani Mountains during the Early Middle Ages: A case study of Contrada Castro, central western Sicily
2022
From 2017, an unknown rural settlement in Contrada Castro at Corleone (Palermo Province, western Sicily) was investigated as part of the `Harvesting Memories Project¿. The stratigraphic sequence, supported by radiocarbon dating, has demonstrated a reoccupation of a pre-Roman site during the transition between the Byzantine and Islamic periods. In particular, the main occupation occurred in the late 8th¿9th century when pottery kilns and a probable warehouse were constructed. During the 10th¿11th century, a new structure with different orientations replaced the previous buildings that had already collapsed. Specifically focusing on a perspective of the household production and its relationsh…
Infantile intranuclear rod myopathy.
1997
This report concerns three unrelated floppy infants, two girls and one boy, each biopsied at the age of 1 month. They were hypotonic since birth and required artificial ventilation. The two girls died at the ages of 4 and 3½ months, respectively, the boy is still alive at the age of 2 years, but requires assisted ventilation. Each of the three infants showed, by muscle biopsy, abundant intranuclear rods, the boy and one girl also had sarcoplasmic rods, which were not present in the other girl's muscle. Absence of sarcoplasmic rods, but the presence of intranuclear rods could also be documented in her autopsied muscle. Using an antibody against α-actinin, immunoelectron microscopy showed re…
Fluorescence In Situ Hybridization (FISH) on Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections
2011
Fluorescence In Situ Hybridization (FISH) is a powerful technique for localizing specific DNA targets directly in the fixed tissue or cells. Bacterial artificial chromosome (BAC) as well as commercial probes, which could be supplied ready for use or concentrated and must be diluted following the manufacturers instructions, can be used. The technique requires 2 days, as an overnight incubation of the FISH probes is needed for optimal hybridization. The critical steps include deparaffinization of tissue sections, optimal pretreatment (target retrieval and protein digestion), and probe hybridization. In this chapter, the described FISH protocol provides a methodology for analyzing the cytogene…
Relationships between soil water repellency and microbial community composition under different plant species in a Mediterranean semiarid forest
2014
Abstract Soil water repellency (SWR) can influence many hydrological soil properties, including water infiltration, uneven moisture distribution or water retention. In the current study we investigated how variable SWR persistence in the field is related to the soil microbial community under different plant species (P. halepensis, Q. rotundifolia, C. albidus and R. officinalis) in a Mediterranean forest. The soil microbial community was determined through phospholipid fatty acids (PLFA). The relationships between microbiological community structure and the soil properties pH, Glomalin Related Soil Protein (GRSP) and soil organic matter (SOM) content were also studied. Different statistical …
Fasti seicenteschi nella scrittura di Gesualdo Bufalino
2009
Il saggio esamina le peculiarità del barocco dissonante di Gesualdo Bufalino. In Bufalino l’artificio dello stile sottende una tensione ideologica. L’intensità espressiva e l’eccesso verbale cercano di compensare un vuoto e costituiscono, nel sentire dello scrittore, un formidabile mezzo «per contrastare l’ossificazione del mondo». Bufalino rivendica quindi il valore strutturale della propria scelta barocca, dichiarando che la sua scrittura è sorretta da un «barocco borrominiano» in cui «l’ornato è funzione», in mancanza del quale «l’architettura cadrebbe».
‘One-Pot’ Synthesis of Raumacline from Ajmaline
1993
For the alkaloid raumacline (2), which is a biotransformation product of ajmaline (1) in Rauwolfia serpentina cell cultures, an efficient ‘one-pot’ synthesis was developed using a NaBH4/riboflavin/light-mediated transformation of 1 into 2 with a total yield of 86%.
Effects of SO2 fumigations on photosynthetic CO2 gas exchange, chlorophyll a fluorescence emission and antioxidant enzymes in the lichens Evernia pru…
1999
The effects of elevated gaseous SO2 concentrations in the lichens Evernia prunastri (L.) Ach. and Ramalina farinacea (L.) Ach. were investigated by means of gas exchange, modulated chlorophyll fluorescence analysis and antioxidant enzyme assays. The response to SO2 of the studied species differed markedly. Net photosynthetic rates were more adversely affected in E. prunastri than in R. farinacea. In addition, processes dependent on thylakoid membrane integrity such as PSII-mediated electron flow and nonphotochemical quenching were reduced to a greater extent by exposure to SO2 in E. prunastri. Moreover, the ability to reoxidize the quinone pool was lower in this species. Finally, the activi…
Lichen rehydration in heavy metal polluted environments: Pb modulates the oxidative response of both Ramalina farinacea thalli and its isolated micro…
2014
Lichens are adapted to desiccation/rehydration and accumulate heavy metals, which induce ROS especially from the photobiont photosynthetic pigments. Although their mechanisms of abiotic stress tolerance are still to be unravelled, they seem related to symbionts' reciprocal upregulation of antioxidant systems. With the aim to study the effect of Pb on oxidative status during rehydration, the kinetics of intracellular ROS, lipid peroxidation and chlorophyll autofluorescence of whole Ramalina farinacea thalli and its isolated microalgae (Trebouxia TR1 and T. TR9) was recorded. A genetic characterization of the microalgae present in the thalli used was also carried out in order to assess possib…
Characterization of the LGBTI-speaking community discourse. An approach to Hispanic queer linguistics.
2020
Toda subcultura forma una comunidad lingüística particular en la que, aunque se comparta la lengua con el resto de la sociedad, varía el habla en ciertos aspectos. Es el caso de la comunidad de habla LGTBI, que se hace servir de un léxico peculiar, así como una fraseología característica, unas estructuras gramaticales especiales y, en definitiva, unos recursos pragmáticos muy llamativos que tienen como objetivo mitigar ciertos enunciados, intensificar otros en determinados contextos y construir actos de habla afiliativos que expresen solidaridad social. Por otro lado, cumple una función muy importante en la construcción identitaria de sus usuarios. En este trabajo se propone un análisis pra…