Search results for "Mitos"
showing 10 items of 190 documents
Inhibitory effect of resveratrol on the proliferation of human and rat hepatic derived cell lines.
2000
Resveratrol is a polyphenolic compound especially produced by grapevine and consequently found in wine. Based on epidemiological studies resveratrol may act as a cancer chemopreventive compound. The ability of resveratrol to inhibit cell proliferation was studied in rat hepatoma Fao cell line and human hepatoblastoma HepG2 cell line. The results show that resveratrol strongly inhibits cell proliferation at the micromolar range in a time- and dose-dependent manner. Concentrations higher than 50 microM become toxic. Fao cells are more sensitive than HepG2 cells. Interestingly, the presence of ethanol lowers the threshold of resveratrol effect. Resveratrol appears to prevent or to delay the en…
Los medios de comunicación escritos virtuales en Internet: calidad científica de la difusión de la hipnosis en castellano durante 2011 y 2012. Estudi…
2017
ABSTRACT Título Los medios de comunicación escritos virtuales en internet: calidad científica de la difusión de la hipnosis en castellano durante 2011 y 2012. Estudio exploratorio desde una perspectiva mixta Introducción La hipnosis ha sido y es un tema polémico tanto en los ámbitos académicos como en la realidad de la calle. Este campo de estudio siempre ha estado rodeado de mitos que magnificaban sus efectos, tanto los positivos como los negativos. Las creencias y expectativas tanto de los pacientes/clientes como de los profesionales capacitados para el uso de la hipnosis influyen directa e indirectamente tanto en la decisión del uso de esta herramienta como sobre el resultado previsible.…
Sequence of lethal events in HeLa cells exposed to the G2 blocking cytolethal distending toxin
2000
The bacterial cytolethal distending toxin (CDT) was previously shown to block the cell cycle of several cell lines at stage G2 through inactivation of the cyclin-dependent kinase Cdkl and without induction of DNA strand breaks. In the present study, we have analyzed, using various methods of analytical cytometry, the progressive transformation and delayed lethal events in the tumor-derived HeLa cell line temporarily exposed to CDT. The cell proliferation arrest induced by CDT was irreversible but, starting about two days after exposure, the G2 block released partially, concomitantly with a decline in the level of Cdkl phosphorylation. This partial release resulted in endoreduplication, lead…
In vivo detection of cytokeratin filament network breakdown in cells treated with the phosphatase inhibitor okadaic acid.
2001
We have previously described vulva carcinoma-derived A-431 subclone AK13-1, which stably expresses fluorescently labeled cytokeratin filaments (CKFs). Time-lapse fluorescence microscopy of these cells permits the continuous monitoring of the dynamics of the CKF cytoskeleton in vivo. To study mechanisms and principles of CKF disassembly as it occurs, e.g., during mitosis and liver disease, we have treated cells with the phosphatase inhibitor okadaic acid (OA), which induces complete CKF network breakdown within 3–5 h without significantly affecting the organization of the actin- and tubulin-based cytofilaments. In time-lapse movies, we find that the network breakdown starts at the cell perip…
Sexism, romantic love and gender inequality. A study of Latin American adolescents living in Spain
2019
Este trabajo examina el papel de los factores sociodemográficos como determinantes de las creencias y actitudes sexistas y las percepciones distorsionadas sobre el amor romántico, y analiza la relación entre el sexismo hostil y el sexismo benevolente y las creencias en mitos del amor romántico, según el Índice de Desigualdad de Género (idg), en una muestra de 203 adolescentes latinoamericanos residentes en Valencia (España). This paper examines the role of socio-demographic factors determining both sexist beliefs and attitudes and distorted perceptions of romantic love. Moreover, the relationship between hostile and benevolent sexism, and the belief in myths about romantic love is analyzed …
The Parasitic Colonization of the Horny Layer in Tinea Versicolor
1972
Observations of vertical sections and of tape strippings of areas infested with tinea versicolor indicate that because of their dimorphic state, these parasites can colonize the horny layer. Mycelial hyphae progress downwards into the intercellular spaces of the horny cells where they feed on nonkeratinous substances and extrude proliferating yeast cells which form clusters in nest-like cavities. The yeast-mycelial conversion gives rise to new mycelial hyphae and the cycle is complete. The parasites are actually lodged in tunnels and nests hollowed out in the horny layer. A dynamic balance between the cycle of the parasites and the renewal rate of the horny layer is responsible for the long…
Growth and macromolecular content of the dimorphic fungus Aureobasidium pullulans and the effect of hydroxyurea and other inhibitors
1988
The growth kinetics and the macromolecular content of the yeast and ethanol-induced hyphal forms of Aureobasidium pullulans were studied. During the morphological transition from yeasts to hyphae, both the protein and RNA content decreased significantly, the mycelial form containing only 76% of the amount of protein in the yeasts, and 38% of the RNA. The DNA was the only component tested whose level increased during the transition. Among several compounds inhibiting macromolecular synthesis, only hydroxyurea showed a remarkable effect on the morphology of A. pullulans, inducing the mycelial morphology. The macromolecular composition of hydroxyurea-treated cultures changed with time in a way…
Transformation of the dermatophyte Trichophyton mentagrophytes to hygromycin B resistance.
1989
A transformation system for the ringworm-producing dermatophyte Trichophyton mentagrophytes has been developed. The system employs the plasmid pHIS, which contains a bacterial hygromycin B phosphotransferase gene linked to Cochliobolus heterostrophus regulatory sequences (B. G. Turgeon, R. C. Garber, and O. C. Yoder, Mol. Cell. Biol. 7:3297-3305, 1987). This plasmid confers hygromycin B resistance to T. mentagrophytes. The DNA was stably integrated into the fungal genome, and the number and sites of integrations varied among transformants. Transformant clones were capable of infecting guinea pigs. This system opens the way for the molecular genetic analysis of the interaction of T. mentagro…
Genome size and chromosomes in marine sponges [Suberites domuncula, Geodia cydonium]
1995
The genome size of the marine sponges Suberites domuncula and Geodia cydonium has been determined by flow cytofluorometric analysis using diamidino-phenylindole [DAPI]. Using human lymphocytes as reference the amount of DNA in cells from S. domuncula has been determined to be 3.7 pg and that of G. cydonium 3.3 pg. While no chromosomes could be identified in G. cydonium, the karyotype of the Suberites domuncula is 32 chromosomes in the diploid state. The size of the chromosomes was between 0.25 and 1.0 micron. No pronounced banding pattern was visible.
Synthesis and evaluation of microtubule assembly inhibition and cytotoxicity of prenylated derivatives of cyclo-l-Trp-l-Pro
2000
The synthesis of three isoprenylated derivatives of cyclo-L-Trp-L-Pro is described. These substances have been evaluated for cytotoxic activity in rat normal fibroblast 3Y1 cells and have also been evaluated in vitro for the inhibition of microtubule assembly.