Search results for "Native"

showing 10 items of 1727 documents

Intragenic G-quadruplex structure formed in the human CD133 and its biological and translational relevance.

2016

Cancer stem cells (CSCs) have been identified in several solid malignancies and are now emerging as a plausible target for drug discovery. Beside the questionable existence of CSCs specific markers, the expression of CD133 was reported to be responsible for conferring CSC aggressiveness. Here, we identified two G-rich sequences localized within the introns 3 and 7 of the CD133 gene able to form G-quadruplex (G4) structures, bound and stabilized by small molecules. We further showed that treatment of patient-derived colon CSCs with G4-interacting agents triggers alternative splicing that dramatically impairs the expression of CD133. Interestingly, this is strongly associated with a loss of C…

cancer stem cells0301 basic medicineDNA damageSettore BIO/11 - Biologia MolecolareTumor initiationBiologyG-quadruplex03 medical and health sciencesCancer stem cellAntigens CDCell Line TumorG-QuadruplexeGeneticsHumansNeoplasm InvasivenessAC133 AntigenGeneGlycoproteinsCell ProliferationSettore MED/04 - Patologia GeneraleNeoplasm InvasiveneG-quadruplexProtein BiosynthesiDrug discoveryGene regulation Chromatin and EpigeneticsAlternative splicingIntroncd133Molecular biologyG-QuadruplexesGene Expression Regulation Neoplastic030104 developmental biologyCell Transformation NeoplasticDrug Resistance NeoplasmProtein BiosynthesisPeptideNeoplastic Stem CellsCancer researchNeoplastic Stem CellSettore MED/46 - Scienze Tecniche Di Medicina Di LaboratorioGlycoproteinPeptidesHuman
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Civilization and sexual abuse: selected Indian captivity narratives and the Native American boarding-school experience

2019

This paper offers a contrastive analysis of Indian captivity narratives and the Native American boarding-school experience. Indian captivity narratives describe the ordeals of white women and men, kidnapped by Indians, who were separated from their families and subsequently lived months or even years with Indian tribes. The Native American boarding-school experience, which began in the late nineteenth century, took thousands of Indian children from their parents for the purpose of “assimilation to civilization” to be facilitated through governmental schools, thereby creating a captivity of a different sort. Through an examination of these two different types of narratives, this paper reveal…

captivity narrativesassimilationHistoryWhite (horse)EthnocentrismCivilizationnative americansLanguage and Literaturemedia_common.quotation_subjectsexual abuseCaptivityCultural assimilationPboarding schoolsSexual abuseGeneral Earth and Planetary SciencesEthnologyNarrativeGeneral Environmental ScienceContrastive analysismedia_commonCrossroads. A Journal of English Studies
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A review of the biological and pharmacological activities of saponins.

1996

Summary This paper reviews the important biological and pharmacological activities of saponins reported over the last few years. These include cancer-related activity, as well as antiphlogistic and antiallergic, immunomodulating, antihepatotoxic, antiviral, hypoglycemic, antifungal and molluscicidal activities. The action of saponins on the cardiovascular, central nervous, and endocrine systems and other miscellaneous effects are also discussed.

carbohydrates (lipids)PharmacologyAntifungalTriterpenoidComplementary and alternative medicinemedicine.drug_classDrug DiscoverymedicinePharmaceutical ScienceMolecular MedicineBiological activityBiologyPharmacologyPhytomedicine : international journal of phytotherapy and phytopharmacology
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The second component of human complement: Detection of two hemolytic forms in plasma by pH Variation

1988

The second component of human complement (C2) in pseudoglobulin prepared from normal plasma eluted as a single peak at high conductivity (30 mS) and pH 4.5 from the cationic exchangers S-Sepharose or Mono S in the Fast Protein Liquid Chromatography (FPLC) System. The C2 was stable at pH 4.5 and 0 degrees C if enzyme inhibitors were used and the pH was raised to 6.0 after elution from the columns. After rechromatography on Mono S in the FPLC System at the median isoelectric point of 5.5 or pH 6.0, the C2 eluted as two distinct hemolytic forms: the first peaked at 16 mS, the second at 30 mS. The two forms of C2 did not correlate with the allotypic variant of C2 in individual, normal human pla…

chemistry.chemical_classificationChromatographyElutionImmunologySize-exclusion chromatographyComplement C4Enzyme-Linked Immunosorbent AssayFast protein liquid chromatographyHematologyComplement C1 Inactivator ProteinsComplement C2Hydrogen-Ion ConcentrationChromatography Ion ExchangeHemolysisComplement factor Bchemistry.chemical_compoundIsoelectric pointEnzymeBiochemistrychemistryAlternative complement pathwayHumansImmunology and AllergySodium dodecyl sulfateImmunobiology
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Characterisation of Diarylheptanoid- and Flavonoid-type Phenolics in Corylus avellana L. Leaves and Bark by HPLC/DAD-ESI/MS

2013

Introduction The leaves of Corylus avellana L. (common hazel, Betulaceae), a plant with a wide distribution in Europe, have been used in folk medicine for various diseases, but phytochemical exploration of C. avellana is still incomplete. To the best of our knowledge there is no previous report concerning diarylheptanoids in C. avellana, although these compounds show a frequent occurrence among Betulaceae plants. Objective To improve existing online chromatographic methods for the investigation of the phenolic compounds in C. avellana leaves and bark, focusing on diarylheptanoid-type molecules. Methods Dried and powdered leaves and bark of C. avellana were extracted with increasing polarity…

chemistry.chemical_classificationChromatographySoxhlet extractorFlavonoidDiarylheptanoidEthyl acetatePlant ScienceGeneral MedicineBiochemistryHigh-performance liquid chromatographyAnalytical Chemistrychemistry.chemical_compoundComplementary and alternative medicinechemistryPhytochemicalvisual_artDrug Discoveryvisual_art.visual_art_mediumMolecular MedicineBarkDiarylheptanoidsFood SciencePhytochemical Analysis
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Investigations on the geographical variability of the human transferrins.

1971

On the basis of own and from the widely scattered literature compiled materials the geographical distribution of the transferrin alleles is discussed. They reveal a characteristic distribution pattern, as the slow Tf variants produced by TfD alleles are obviously more frequent in tropical than in non-tropical populations. Considering similar results in cattle and its interpretation by Ashton (1958, 1965), it is hypothesized, that the relatively high TfΓ frequencies in tropical biotops might be a selective adaptation to their special climatic conditions. In this connection an association between slowly moving transferrin proteins in man and tolerance to hotter climates — as could be found in…

chemistry.chemical_classificationGeneticsPhysiological functionTropical ClimateNative Hawaiian or Other Pacific IslanderGeographyRacial GroupsTransferrinBlack PeopleGenetic VariationBiologyWhite PeopleGenetics PopulationCharacteristic distributionchemistryAsian PeopleEvolutionary biologyTransferrinInuitGeneticsHumansAlleleAdaptationGenetics (clinical)AllelesHumangenetik
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Anodic Dehydrogenative Cyanamidation of Thioethers: Simple and Sustainable Synthesis of N‐Cyanosulfilimines

2021

Abstract A novel and very simple to perform electrochemical approach for the synthesis of several N‐cyanosulfilimines in good to excellent yields was established. This method provides access to biologically relevant sulfoximines by consecutive oxidation using electro‐generated periodate. This route can be easily scaled‐up to gram quantities. The S,N coupling is carried out at an inexpensive carbon anode by direct oxidation of sulfide. Therefore, the designed process is atom economic and represents a new “green route” for the synthesis of sulfilimines and sulfoximines.

chemistry.chemical_classificationGreen chemistry540 Chemistry and allied sciencesSulfideElectroorganic Synthesisgreen chemistryCommunicationchemistry.chemical_elementPeriodateGeneral Chemistryoxidative imidationElectrochemistryCombinatorial chemistryCatalysisCommunicationsAnodechemistry.chemical_compoundchemistryelectrochemistrySimple (abstract algebra)540 Chemiedehydrogenative couplingCarbonsulfiliminesAngewandte Chemie (International Ed. in English)
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Evaluating chemical ligation techniques for the synthesis of block copolypeptides, polypeptoids and block copolypept(o)ides: a comparative study

2015

In this work, we describe the synthesis of block copolypeptides, polypeptoids and block copolypept(o)ides by chemical ligation techniques. Polysarcosine (PSar), poly(N-e-trifluoroacetyl-L-lysine) (PLys(TFA)) and poly(γ-benzyl-L-glutamate) (PGlu(OBzl)) homopolymers of different polarities and end group functionalities but with similar average degrees of polymerization (Xn = 50 and 100) could be obtained by ring opening polymerization (ROP) of α-amino acid N-carboxyanhydrides (NCA) and postpolymerization modification reactions. In the next step, these polymers were applied to copper(I)-catalyzed azide–alkyne coupling (CuAAC), strain-promoted azide–alkyne coupling (SPAAC) and native chemical l…

chemistry.chemical_classificationPolymers and PlasticsOrganic ChemistryBioengineeringPolymerNative chemical ligationBiochemistryCombinatorial chemistryRing-opening polymerizationchemistry.chemical_compoundEnd-groupPolymerizationchemistryPeptide synthesisChemical ligationLigationPolymer Chemistry
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The Anaphylatoxic Peptide C3a of Guinea Pig Complement

1978

Abstract Highly purified guinea pig C3a was obtained after specific cleavage of isolated C3 by the alternative pathway enzyme VF-B in a one step procedure. It turned out to be a low molecular weight peptide with basic character (M.W. 9500; isoelectric point above 9.4). C3a represents an antigenetic determinant of its own in the native C3 molecule, different from the B determinant. Guinea pig C3a is resistant to 100°C for 10 minutes. Its smooth muscle contracting activity can be destroyed by trypsin and carboxypeptidase B. These findings indicate that guinea pig C3a is quite similar to human C3a.

chemistry.chemical_classificationbiologyChemistrychemical and pharmacologic phenomenaPeptideGeneral MedicineCleavage (embryo)TrypsinMolecular biologyCarboxypeptidaseGuinea pigIsoelectric pointEnzymeBiochemistryAlternative complement pathwaymedicinebiology.proteinmedicine.drugZeitschrift für Immunitätsforschung: Immunobiology
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LC-NMR applied to the characterisation of cardiac glycosides from three micropropagatedIsoplexisspecies

2002

Species of the genus Isoplexis are of particular interest with respect to the biochemical pathway leading to the cardenolides. It is important to determine whether or not 5β-configured compounds, typically produced by Digitalis species and used in medicine, are present together with their respective α-isomers in Isoplexis spp. Structure elucidation by LC-NMR of the products isolated from in vitro regenerated Isoplexis canariensis, I. chalcantha and I. isabelliana was carried out, and similarities were observed among the products of the three species, including the presence of digitoxigenin-type cardenolides in I. canariensis and xysmalogenin and canarigenin derivatives in I. chalcantha neve…

chemistry.chemical_classificationbiologyStereochemistryScrophulariaceaePregnaneGlycosideDigitalisPlant ScienceGeneral Medicinebiology.organism_classificationBiochemistryAnalytical ChemistryIsoplexis canariensisCanarigeninchemistry.chemical_compoundComplementary and alternative medicinechemistryGenusDrug DiscoveryIsoplexisBotanyMolecular MedicineFood SciencePhytochemical Analysis
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