Search results for "PHOSPHATASE"
showing 10 items of 499 documents
PON3 is upregulated in cancer tissues and protects against mitochondrial superoxide-mediated cell death
2012
To achieve malignancy, cancer cells convert numerous signaling pathways, with evasion from cell death being a characteristic hallmark. The cell death machinery represents an anti-cancer target demanding constant identification of tumor-specific signaling molecules. Control of mitochondrial radical formation, particularly superoxide interconnects cell death signals with appropriate mechanistic execution. Superoxide is potentially damaging, but also triggers mitochondrial cytochrome c release. While paraoxonase (PON) enzymes are known to protect against cardiovascular diseases, recent data revealed that PON2 attenuated mitochondrial radical formation and execution of cell death. Another famil…
Can Alzheimer disease be a form of type 3 diabetes?
2012
Alzheimer disease (AD) and metabolic syndrome are two highly prevalent pathological conditions of Western society due to incorrect diet, lifestyle, and vascular risk factors. Recent data have suggested metabolic syndrome as an independent risk factor for AD and pre-AD syndrome. Furthermore, biological plausibility for this relationship has been framed within the “metabolic cognitive syndrome” concept. Due to the increasing aging of populations, prevalence of AD in Western industrialized countries will rise in the near future. Thus, new knowledge in the area of molecular biology and epigenetics will probably help to make an early molecular diagnosis of dementia. An association between metabo…
CtsR is the master regulator of stress response gene expression in Oenococcus oeni.
2005
ABSTRACT Although many stress response genes have been characterized in Oenococcus oeni , little is known about the regulation of stress response in this malolactic bacterium. The expression of eubacterial stress genes is controlled both positively and negatively at the transcriptional level. Overall, negative regulation of heat shock genes appears to be more widespread among gram-positive bacteria. We recently identified an ortholog of the ctsR gene in O. oeni . In Bacillus subtilis , CtsR negatively regulates expression of the clp genes, which belong to the class III family of heat shock genes. The ctsR gene of O. oeni is cotranscribed with the downstream clpC gene. Sequence analysis of t…
Zum polymorphismus der alkalischen phosphatase (EC3.1.3.1.)
1976
1. Plazenta-Extrakte weisen in der horizontalen Starkegel-Elektrophorese einen Polymorphismus der alkalischen Phosphatase auf, der nach ROBSON und HARRIS vom Genotyp des Feten bestimmt wird. In 73 untersuchten Plazentaproben wurden 5 der 6 haufigen Phanotypen beobachtet, deren Frequenz sehr gute Ubereinstimmung mit den Werten der englischen Autoren aufwies. Die Typisierung war allerdings wegen undeutlicher Darstellung der Muster sehr schwierig. 2.In 66 Seren von Muttern wurde zum Geburtstermin zwar auch ein Polymorphismus der hitzestabilen alkalischen Phosphatase beobachtet, der jedoch nur 4 Typen umfaste und keine sichere Zuordnung zu den Plazentamustern gestattete. 3. Die 66 untersuchten …
Influence of partial O 2 pressure on the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells on β-tricalcium phos…
2018
Our purpose was to analyse the influence of O2 pressure on the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells (DPSC) on 3-tricalcium phosphate (3-TCP) scaffold. DPSC isolated from extracted third molars were seeded on 3-TCP and cultured under 3% or 21% O2 pressure. Cell adhesion, proliferation, and differentiation over the biomaterial were evaluated at 7, 13, 18, and 23 days of culture. Cell adhesion was determined by light microscopy, proliferation by DNA quantification, and osteogenic differentiation by alkaline phosphatase activity analysis. All DPSC adhered to 3-TCP with both O2 conditions. Cell proliferation values were higher at 3% O2 in each t…
Control of the mutagenicity of aromatic amines by protein kinases and phosphatases
1997
The role of protein kinase C and protein phosphatases was examined in the control of mutagenic metabolites of aromatic amines. Various metabolic activating systems derived from rat liver were treated with: 12-O-tetradecanoylphorbol-13-acetate (TPA), a protein kinase C modulator; okadaic acid (OA), a potent inhibitor of serine/threonine protein phosphatases (PP1 and PP2A); and ortho-vanadate (OV), an inhibitor of tyrosine phosphatases. TPA used over a wide concentration range (10−9–10−6 M) did not affect the bacterial mutagenicity of the aromatic amines and of the aromatic amide investigated, 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene (2AAF). At the molecular level, TPA did…
Self-adjuvanting C18 lipid vinil sulfone-PP2A vaccine: study of the induced immunomodulation against
2017
Despite the importance of the adjuvant in the immunization process, very few adjuvants merge with the antigens in vaccines. A synthetic self-adjuvant oleic-vinyl sulfone (OVS) linked to the catalytic region of recombinant serine/threonine phosphatase 2A from the nematode Angiostrongylus costaricensis (rPP2A) was used for intranasal immunization in mice previously infected with Trichuris muris. The animal intranasal immunization with rPP2A-OVS showed a reduction of 99.01% in the number of the nematode eggs and 97.90% in adult. The immunohistochemical analysis of the intestinal sections showed that in immunized animals with lipopeptide the mucus was significantly higher than in the other expe…
The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors
2010
Abstract ISWI is an evolutionarily conserved ATP-dependent chromatin remodeling factor playing central roles in DNA replication, RNA transcription, and chromosome organization. The variety of biological functions dependent on ISWI suggests that its activity could be highly regulated. Our group has previously isolated and characterized new cellular activities that positively regulate ISWI in Drosophila melanogaster. To identify factors that antagonize ISWI activity we developed a novel in vivo eye-based assay to screen for genetic suppressors of ISWI. Our screen revealed that ISWI interacts with an evolutionarily conserved network of cellular and nuclear factors that escaped previous genetic…
Use of sonication for measuring acid phosphatase activity in soil
2000
Extracellular enzymes in soil often occur in immobilised forms, a state that may alter their interactions with substrates in comparison with enzymes in the solution phase. Sonication was evaluated for its usefulness in studying immobilised acid phosphatase by dispersing soil aggregates. Factors affecting soil dispersion during ultrasound application were soil extraction ratio, total applied energy and power output ml−1 of sonicated soil slurry. For the clay loam soil used, optimal values for these variables were, respectively, 1:6 (w/v) and, at least, 1800 J ml−1 and 15 W ml−1. At the optimal sonication conditions for soil dispersion a substantial increase in phosphatase activity (up to 156…
Effects of a phycotoxin, okadaic acid, on oyster heart cell survival
2008
Okadaic acid (OA) is a dinoflagellate toxin which accumulates in shellfish producing diarrhetic shellfish poisoning (DSP) in humans. It was found that OA is a highly selective inhibitor of protein phosphatase types 1 (PP1) and 2A (PP2A) which produces a marked increase in phosphorylation of several proteins, including p38 mitogen-activated protein (MAP) kinase. The cytotoxicity attributed to OA and the effects on p38 MAP kinase and calcium current were examined in the oyster Crassostrea gigas in this study. Data showed that p38 MAP kinase is strongly expressed in oyster heart and that OA bioaccumulated in cultured heart cells. Hence the effects of OA was tested in vitro and in vivo on oyste…