Search results for "Polyad"

Alternative Polyadenylation Events Contribute to the Induction of NF-ATc in Effector T Cells

1999

Abstract The transcription factor NF-ATc is synthesized in three prominent isoforms. These differ in the length of their C terminal peptides and mode of synthesis. Due to a switch from the use of a 3′ polyA site to a more proximal polyA site, NF-ATc expression switches from the synthesis of the two longer isoforms in naive T cells to that of short isoform A in T effector cells. The relative low binding affinity of cleavage stimulation factor CstF-64 to the proximal polyA site seems to contribute to its neglect in naive T cells. These alternative polyadenylation events ensure the rapid accumulation of high concentrations of NF-ATc necessary to exceed critical threshold levels of NF-ATc for g…

Characterization of a Cu/Zn Superoxide dismutase-encoding gene region in Drosophila willistoni

1994

A Cu/Zn superoxide dismutase-encoding gene (Sod) from Drosophila willistoni was cloned and sequenced. The gene shows a typical structure for a fruit-fly Sod gene, with a coding region of 462 bp in two exons separated by a 417-bp intron. Comparison of the Sod sequences from D. willistoni and D. melanogaster suggests that these species are only remotely related. Downstream from the Sod gene, there is an ORF on the opposite strand that putatively encodes the last exon of an unidentified gene. The polyadenylation signals of the two genes are separated by only 61 bp in D. willistoni, conforming to the common picture of compact dipteran genomes.

High Resolution Study of the v1 + 2v2 −v2 and 2v2 + v3 − v2 ”Hot” Bands and Ro-Vibrational Re-Analysis of the v1 + v2/v2 + v3/3v3 Poliad of the SO2 M…

2010

The main goal of the present study was to analyse rotational stuctures of two excited vibrational states, (120) and (021). Because both the band, v1+2v2 and 2v2+v3 one are extremally weak, a special efforts were faired to record very weak hot bands, v1+2v2−v2 and 2v2+v3−v2.Both of those bands are located in the region of considerably more stronger combinational bands, v1 + v2 and v2 + v3. On that reason, as the first step of analysis, we made assignments of transitions belonging to these v1 + v2 and v2 + v3 bands. As the result of analysis, we were able to assign three times more transitions to the bands v1 + v2 and v2 + v3 than it was known in the before literature. After "cleaning" the ex…

Multi-subject fMRI analysis via combined independent component analysis and shift-invariant canonical polyadic decomposition

2014

Canonical polyadic decomposition (CPD) may face a local optimal problem when analyzing multi-subject fMRI data with inter-subject variability. Beckmann and Smith proposed a tensor PICA approach that incorporated an independence constraint to the spatial modality by combining CPD with ICA, and alleviated the problem of inter-subject spatial map (SM) variability.This study extends tensor PICA to incorporate additional inter-subject time course (TC) variability and to connect CPD and ICA in a new way. Assuming multiple subjects share common TCs but with different time delays, we accommodate subject-dependent TC delays into the CP model based on the idea of shift-invariant CP (SCP). We use ICA …

LPS challenge regulates gene expression and tissue localization of a Ciona intestinalis gene through an alternative polyadenylation mechanism

2013

LPS injection reprograms the expression and the 3′ UTR of a CAP gene by alternative polyadenylation and the formation of a GAIT element in Ciona inte…

2016

The diversification of cellular functions is one of the major characteristics of multicellular organisms which allow cells to modulate their gene expression, leading to the formation of transcripts and proteins with different functions and concentrations in response to different stimuli. CAP genes represent a widespread family of proteins belonging to the cysteine-rich secretory protein, antigen 5 and pathogenesis-related 1 superfamily which, it has been proposed, play key roles in the infection process and the modulation of immune responses in host animals. The ascidian Ciona intestinalis represents a group of proto-chordates with an exclusively innate immune system that has been widely st…

LPS challenge regulates gene expression and tissue localization of a Ciona intestinalis gene through an alternative polyadenylation mechanism.

2013

A subtractive hybridization strategy for the identification of differentially expressed genes was performed between LPS-challenged and naive Ciona intestinalis. This strategy allowed the characterization of two transcripts (Ci8short and Ci8long) generated by the use of two Alternative Polyadenylation sites. The Ci8long transcript contains a protein domain with relevant homology to several components of the Receptor Transporting Protein (RTP) family not present in the Ci8short mRNA. By means of Real Time PCR and Northern Blot, the Ci8short and Ci8long transcripts showed a different pattern of gene expression with the Ci8short mRNA being strongly activated after LPS injection in the pharynx. …

Symplekin, a polyadenylation factor, prevents MOZ and MLL activity on HOXA9 in hematopoietic cells

2013

International audience; MOZ and MLL encoding a histone acetyltransferase and a histone methyltransferase, respectively, are targets for recurrent chromosomal translocations found in acute myeloblastic or lymphoblastic leukemia. We have previously shown that MOZ and MLL cooperate to activate HOXA9 gene expression in hematopoietic stem/progenitors cells. To dissect the mechanism of action of this complex, we decided to identify new proteins interacting with MOZ. We found that the scaffold protein Symplekin that supports the assembly of polyadenylation machinery was identified by mass spectrometry. Symplekin interacts and co-localizes with both MOZ and MLL in immature hematopoietic cells. Its …

A high-fat diet increases l-carnitine synthesis through a differential maturation of the Bbox1 mRNAs.

2013

International audience; l-carnitine is a key molecule in both mitochondrial and peroxisomal lipid metabolisms. l-carnitine is biosynthesized from gamma-butyrobetaine by a reaction catalyzed by the gamma-butyrobetaine hydroxylase (Bbox1). The aim of this work was to identify molecular mechanisms involved in the regulation of l-carnitine biosynthesis and availability. Using 3' RACE, we identified four alternatively polyadenylated Bbox1 mRNAs in rat liver. We utilized a combination of in vitro experiments using hybrid constructs containing the Bbox1 3' UTR and in vivo experiments on rat liver mRNAs to reveal specificities in the different Bbox1 mRNA isoforms, especially in terms of polyadenyla…

Modelling polycarbonate synthesis rates on the example of bulk heteropolyaddition of diphenyl carbonate and bisphenol A diglycidyl ether

2016

This paper investigates the effects of increased reaction mixture viscosity on the kinetics of linear polymer creation in a bulk polyaddition process of diphenyl carbonate and bisphenol A diglycidyl ether. The paper presents a method for solving a system of bulk polyaddition of diphenyl carbonate and bisphenol A diglycidyl ether process balance equations, allowing the determinatiof the process kinetic parameters. Determination of polymerisation reaction kinetic parameters was also made possible by the use of the so-called partial reaction rate constant. Such an approach enabled a significant simplification of the mathematical expressions describing the heteropolyaddition process and provide…