Search results for "Vivo"

Characterization of intestinal absorption of C -glycoside flavonoid vicenin-2 from Lychnophora ericoides leafs in rats by nonlinear mixed effects mod…

2015

Vicenin-2 (apigenin-6,8-di-C-β-d-glucopyranoside) is present in hydroalcoholic extracts of the Brazilian species Lychnophora ericoides Mart., Asteraceae, leaves, and the biological effects of this compound have been demonstrated including anti-inflammatory, antioxidant and anti-tumor effects in rat models. Given the potential of this compound as a pharmacological agent, the aims of this investigation were to evaluate the extent of intestinal absorption of vicenin-2, and to determine the intestinal permeation profile using an in situ single-pass intestinal perfusion technique. A validated HPLC–UV method was applied to measure the amount of unabsorbed vicenin-2 in the gut after an oral admini…

Multispectral fluorescence detection of pigmented cutaneous tumours

2020

We investigated pigmented skin tumour lesions in vivo and ex vivo, including benign and dysplastic nevi, as well as malignant lesions, such as pigmented basal cell carcinoma (BCC) and malignant melanoma (MM) lesions, to obtain a complex view about the feasibility of different excitation sources solely and/or in combination to induce fluorescence signal useful for diagnosis of various low-fluorescent cutaneous neoplasia. A specialized multispectral analysis of the data obtained was applied by using excitation in broad spectral range, covering ultraviolet, visible and near-infrared spectral range, that contribute considerably to: (1) fundamental determination of tumour tissues’ spectral prope…

Glucose, Lactate, and Ketone Body Utilization by Human Mammary Carcinomas in Vivo

1985

Uncontrolled growth, one of the fundamental properties of malignant tumors, requires a great supply of energy. This energy can be derived from the use of a variety of substrates. Besides glucose oxidation and glucose breakdown to lactic acid, the turnover of endogeneous substrates such as amino acids, free fatty acids and ketone bodies is well documented in vitro. However, under in vivo conditions, only glucose utilization has been investigated in detail, using tumor isotransplants in rodents. For human tumors, only scarce data is available, derived mainly from clinical observations rather than from systematic studies.

Bovine lactoferrin pepsin hydrolysate exerts inhibitory effect on angiotensin I-converting enzyme-dependent vasoconstriction

2007

Abstract The inhibitory effect of a pepsin hydrolysate of bovine lactoferrin (LFH) on angiotensin I-converting enzyme (ACE) has been examined using in vitro and ex vivo functional assays. In vitro assays showed a LFH inhibitory effect on ACE activity with an IC 50 value of 0.95±0.06 mg mL −1 . Ex vivo functional assays using rabbit carotid artery segments showed a LFH inhibitory effect on ACE-dependent angiotensin I-induced contraction, but not on angiotensin II-induced contraction, suggesting that the effect of LFH is not due to antagonism of receptors for angiotensin II. LFH was shown to possess ACE inhibitory effect with potential to modulate hypertension, although the possible inhibitor…

Inverse correlated 1H-13C in vivo NMR as a probe to follow the metabolism of unlabeled vanillin by plant cell cultures

1997

Abstract Inverse correlated 2D 1H-13C NMR spectroscopy was used to follow the metabolism of vanillin (1) in vivo by plant cell suspension cultures of Nicotiana plumbaginifolia at the natural abundance of 13C. Under the non-invasive conditions four products could be detected: vanillylalcohol (2), vanillinglucoside (3) and the phenolic and benzylic glucosides of vanillylalcohol (4 and 5).

Changes in collagen cross-linking of articular cartilage are revealed by spectral reflectance imaging

2012

Excessive cross-linking of collagen during aging may contribute to degeneration of articular cartilage. Traditionally, the amount of cross-links is derived by using destructive high-performance liquid chromatography (HPLC). However, a sensitive, non-destructive method could help to evaluate tissue integrity, including cross-links. We increased collagen cross-linking in bovine articular cartilage by controlled threose incubation. During the incubation, optical spectral reflectance images of the samples were captured and related to HPLC results using regression analysis. Significant correlations with reflectance and cross-links were observed. When further developed the optical method may enab…

The role of thrombospondin-1 in biomaterial integration of porous polyethylene implants in vivo

2019

Rational Species Extrapolation of Toxic Effects

1994

One of the main problems in toxicology and pharmacology is the extrapolation from one species to another, particularly from laboratory animals to man or in ecotoxicology from laboratory animals to free living species. Many toxicological data were raised only in one or a few species of laboratory animals. In the last decades more and more experimental data were obtained from in vitro-systems. Therefore extrapolations from in vitro to in vivo and from species to species are required. Species differences in uptake, distribution, metabolism, site of action, elimination and accumulation have to be taken into account. Most foreign compounds are metabolically transformed by diverse xenobiotica met…

ChemInform Abstract: 2-Sulfonyliminodihydropyrimidines: A Novel Class of Analgesic Compounds.

2009

A series of 2-sulfonyliminodihydropyrimidine derivatives have been synthesized and evaluated in vivo for their antinociceptive and anti-inflammatory activities. The results were compared with that of acetyl salicylic acid. Compounds 6Ab-d and 6Be displayed an interesting analgesic profile in the acetic acid-induced abdominal contractions test. Based on the results of the carrageenan-hind paw edema test, compound 6Af showed potential anti-inflammatory activity.

Influence of bacteria on the metabolite pattern of phenol in zebra fish (Brachydanio rerio)

1989

Summary An investigation was performed on the potential influence of bacteria in the medium on the metabolism of phenol in zebra fish. The results obtained in vitro and in vivo show that bacteria may influence biotransformation studies of xenobiotics by fish in different manner. First, it was shown that phenol conjugates were deconjugated. Hydrolysis of phenyl glucuronide was observed both in vitro and in vivo. Deconjugation of phenyl sulphate was found to a minor extent merely under in vitro conditions. Second, the xenobiotic itself, in this case phenol, was degradated to CO2. The proportion did not lead to a significant removal of the test compound of the fish. Zusammenfassung Einflus von…