Search results for "XYLOGLUCAN"

Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing.

2021

Abstract Hydrogel wound dressings can play critical roles in wound healing protecting the wound from trauma or contamination and providing an ideal environment to support the growth of endogenous cells and promote wound closure. This work presents a self-assembling hydrogel dressing that can assist the wound repair process mimicking the hierarchical structure of skin extracellular matrix. To this aim, the co-assembly behaviour of a carboxylated variant of xyloglucan (CXG) with a peptide amphiphile (PA-H3) has been investigated to generate hierarchical constructs with tuneable molecular composition, structure, and properties. Transmission electron microscopy and circular dichroism at a low c…

A xyloglucan from persimmon fruit cell walls

1998

Persimmon (Diospyros kaki L.) fruit cell walls have been shown to contain a high proportion of xyloglucan. This hemicellulosic polysaccharide appears to play a crucial role in the softening process that accompanies fruit ripening. In the present work, xyloglucan from persimmon fruit cell walls was extracted, purified and chemically characterized from alkali-extracted hemicelluloses. Monosaccharide analysis of purified xyloglucan showed a molar ratio of Glc:Xyl:Gal:Fuc of 10.0:6.0:3.4:1.4. Linkage analysis showed a high proportion of terminal residues, indicating a low degree of polymerization of side-chains.

Data concerning the protein absorption and retention properties of xyloglucan-based hydrogel film

2018

In wound dressing applications, exudate absorption and retention are important properties. The data presented here assess the ability of the crosslinked xyloglucan-poly(vinyl alcohol) hydrogel films (XG-PVA), described in "Xyloglucan-based hydrogel films for wound dressing: Structure-property relationships" (Ajovalasit et al., 2018) [1] and "Biocompatibility, hemocompatibility and antimicrobial properties of xyloglucan-based hydrogel film for wound healing application" (Picone et al., 2019), to absorb and retain proteins. These properties were investigated by Comassie blue staining and electrophoresis of Fetal Serum Proteins. (C) 2018 The Authors. Published by Elsevier Inc. This is an open …

Multi-scale structural analysis of xyloglucan colloidal dispersions and hydro-alcoholic gels

2020

Xyloglucans are highly branched, hydroxyl rich polyglucans that for their abundance in nature, biocompatibility, film forming and gelation ability may take a prominent role in the design and fabrication of biomedical devices, including in situ forming scaffolds for tissue engineering, wound dressings and epidermal sensors. The understanding and exploitation of their self-assembly behavior is key for the device performance optimization. A multi-scale analysis, conducted combining small-angle X-ray scattering, both static and dynamic light scattering at large and small angles, and rheological measurements, provides a description of the supramolecular organization of this biopolymer, from the …

Adipose stem cell spheroids-laden hydrogels for minimally invasive bone and cartilage regeneration interventions

2021

Expression of xyloglucan endotransglucosylase/hydrolase (XTH) genes and XET activity in ethylene treated apple and tomato fruits.

2013

[EN] Xyloglucan endotransglucosylase/hydrolase (XTHs: EC 2.4.1.207 and/or EC 3.2.1.151), a xyloglucan modifying enzyme, has been proposed to have a role during tomato and apple fruit ripening by loosening the cell wall. Since the ripening of climacteric fruits is controlled by endogenous ethylene biosynthesis, we wanted to study whether XET activity was ethylene-regulated, and if so, which specific genes encoding ripening-regulated XTH genes were indeed ethylene-regulated. XET specific activity in tomato and apple fruits was significantly increased by the ethylene treatment, as compared with the control fruits, suggesting an increase in the XTH gene expression induced by ethylene. The 25 Sl…

Apple (Malus domestica) and Tomato (Lycopersicum esculentum) Fruits Cell-Wall Hemicelluloses and Xyloglucan Degradation during Penicillium expansum I…

2004

We characterized the changes in cell-wall hemicellulosic polysaccharides and the hemicellulose-degrading enzymes associated with apple and tomato fruits infected by Penicillium expansum. Our results showed a reduction in the molecular mass of hemicelluloses, with this reduction being particularly notable in the xyloglucan associated with P. expansum infection. The activation of fungal beta-glucanases was also highlighted. Fruit xyloglucan endotransglucosylase/hydrolase (XTH)-specific activity decreased drastically during the infection process in both apple and tomato fruits. We suggest that XTH reduction during the infection might be related with the fungus attack mechanism. We also suggest…

Development and characterization of xyloglucan-poly(vinyl alcohol) hydrogel membrane for Wireless Smart wound dressings

2018

Abstract Hydrogel-based smart wound dressings that combine the traditional favourable properties of hydrogels as skin care materials with sensing functions of relevant biological parameters for the remote monitoring of wound healing are under development. In particular, lightweight, ultra-high frequency radiofrequency identification (UHF RFID) sensor are adjoined to xyloglucan-poly(vinyl alcohol) hydrogel films to battery-less monitor moisture level of the bandage in contact with the skin, as well as wireless transmit the measured data to an off-body reader. This study investigates the swelling behavior of the hydrogels in contact with simulated biological fluids, and the modification of th…

Physico-chemical and mechanical characterization of in-situ forming xyloglucan gels incorporating a growth factor to promote cartilage reconstruction

2016

Abstract The development of growth factors is very promising in the field of tissue regeneration but specifically designed formulations have to be developed in order to enable such new biological entities (NBEs). In particular, the range of therapeutic concentrations is usually very low compared to other active proteins and the confinement in the target site can be of crucial importance. In-situ forming scaffolds are very promising solutions for minimally invasive intervention in cartilage reconstruction and targeting of NBEs. In this work injectable, in-situ forming gels of a temperature responsive partially degalactosylated xyloglucan (Deg-XG) incorporating the growth factor FGF-18 are fo…

Overexpression of a cell wall enzyme reduces xyloglucan depolymerization and softening of transgenic tomato fruits.

2010

Xyloglucan xyloglucosyltransferase/endohydrolase (XTHs: EC 2.4.1.207 and/or EC 3.2.1.151) has been proposed to have a dual role integrating newly secreted xyloglucan chains into an existing wall-bound xyloglucan and restructuring existing cell wall material by catalyzing transglucosylation between previously wall bound xyloglucan molecules. In this work we generated transgenic tomatoes with altered levels of an XTH gene. These transgenic fruits showed significant overexpression of the XTH proteins in comparison with the wild type. Specific XET activity was approximately 4.33 fold higher in the transgenic fruits compared with the wild type fruits, although in both cases the activity decrease…