Search results for "drosophila"
showing 10 items of 782 documents
Tirant is a new member of the gypsy family of retrotransposons in Drosophila melanogaster.
2000
In this paper, we propose a consensus sequence for a putative complete Tirant retrotransposon. Several defective copies, as well as relevant sequences available in databases have been analyzed. The putative complete Tirant element is 8533 bp long, and presents all the structural features of a retroviruslike transposable element of the gypsy family. It contains three ORFs (open reading frames) that encode putative products resembling the retroviral Gag, Pol, and Env proteins. Southern blot analyses show that complete and defective Tirant elements are widespread in Drosophila melanogaster. The different hybridization patterns observed in several natural populations of this species suggest tha…
bilbo, a non-LTR retrotransposon of Drosophila subobscura: a clue to the evolution of LINE-like elements in Drosophila
1997
We used the repetitive character of transposable elements to isolate a non-LTR retrotransposon in Drosophila subobscura. bilbo, as we have called it, has homology to TRIM and LOA elements. Sequence analysis showed a 5' untranslated region (UTR), an open reading frame (ORF) with no RNA-binding domains, a downstream ORF that had structural homology to that of the I factor, and, finally, a 3' UTR which ended in several 5-nt repeats. The results of our phylogenetic and structural analyses shed light on the evolution of Drosophila non-LTR retrotransposons and support the hypothesis that an ancestor of these elements was structurally complex.
Drosophila melanogaster histone H2B retropseudogene is inserted into a region rich in transposable elements.
1998
We have isolated and characterized the genomic sequence of a Drosophila melanogaster histone H2B pseudogene that is localized outside of the cluster of the replication-dependent histone genes and has all the properties of a retropseudogene. It is highly homologous to the transcribed region of the D. melanogaster histone H2B gene, but not to its flanking regions, and is surrounded by short direct repeats. The pseudogene contains several point mutations that preclude its translation. The sequence of the 3' region of this pseudogene is compatible with the hypothesis that the 3' terminal stem-loop structure of the histone H2B mRNA has served as a primer for the reverse transcription event from …
Effect of tin and lead chlorotriphenyl analogues on fruit fly Drosophila hydei and liposomes membrane
2011
This article presents the results of a study investigating the biological activity of triphenyltin chloride (TPhT) and two metalloorganic compounds, triphenyllead chloride (TPhL) and triphenylmethane chloride (TPhC), in their interaction with model membranes and the living organisms of fruit flies Drosophila hydei. The study of model membranes (sonicated liposomes) was conducted using the electron spin resonance (ESR) spin probe technique, whereas the experiment on fruit flies involved investigating their viability on media containing the studied compounds. The test results clearly demonstrate that TPhT affects fruit flies more actively than TPhL (complete lethality after 7 days of culture …
A Spinal Muscular Atrophy Reporter System for in vivo Drug Discovery in Drosophila melanogaster
2018
Fondo La Atrofia Muscular Espinal es un desorden neuromuscular raro y fatal causado por la pérdida o reducción en los niveles de proteína de la Neurona Motor de Supervivencia (SMN). Los individuos afectados tienen el gen SMN1 mutado y la copia SMN2 específica de humanos no afectada, que se traduce solo parcialmente en una proteína SMN funcional. La activación farmacológica de la inclusión del exón 7 de SMN2 por moléculas pequeñas o oligonucleótidos antisentido modificados es un enfoque prometedor para tratar la SMA. Obtener nuevos compuestos potencialmente terapéuticos para los ensayos clínicos es un proceso largo y costoso. El enfoque de reposicionamiento de medicamentos puede reducir el t…
Implicación de miRNAs en la toxicidad mediada por expansiones de repeticiones CTG en Distrofia Miotónica
2015
La distrofia miotónica tipo 1 (DM1) es una enfermedad neuromuscular causa por la expansión del triplete CTG en la región 3’ no codificante del gen DMPK. Las expansiones CUG en los transcritos DMPK mutantes forman una estructura en horquilla que secuestra diferentes factores nucleares provocando su falta de función parcial y la desregulación de la expresión génica a diferentes niveles. La mayoría de los defectos en la expresión génica se han reproducido en animales modelo para la enfermedad que expresan transcritos con repeticiones CUG de manera independiente a DMPK. En este trabajo nos propusimos analizar si un grupo de reguladores de la expresión génica está afectado por la toxicidad media…