Search results for "ingeniería"
showing 10 items of 112 documents
End-to-end congestion control protocols for remote programming of robots, using heterogeneous networks: A comparative analysis
2008
There are many interesting aspects of Internet Telerobotics within the network robotics context, such as variable bandwidth and time-delays. Some of these aspects have been treated in the literature from the control point of view. Moreover, only a little work is related to the way Internet protocols can help to minimize the effect of delay and bandwidth fluctuation on network robotics. In this paper, we present the capabilities of TCP, UDP, TCP Las Vegas, TEAR, and Trinomial protocols, when performing a remote experiment within a network robotics application, the UJI Industrial Telelaboratory. Comparative analysis is presented through simulations within the NS2 platform. Results show how th…
Bifunctional poly(acrylamide) hydrogels through orthogonal coupling chemistries
2019
Biomaterials for cell culture allowing simple and quantitative presentation of instructive cues enable rationalization of the interplay between cells and their surrounding microenvironment. Poly(acrylamide) (PAAm) hydrogels are popular 2D-model substrates for this purpose. However, quantitative and reproducible biofunctionalization of PAAm hydrogels with multiple ligands in a trustable, controlled, and independent fashion is not trivial. Here, we describe a method for bifunctional modification of PAAm hydrogels with thiol- and amine- containing biomolecules with controlled densities in an independent, orthogonal manner. We developed copolymer networks of AAm with 9% acrylic acid and 2% N-(4…
Microenvironments to study migration and somal translocation in cortical neurons
2018
Migrating post-mitotic neurons of the developing cerebral cortex undergo terminal somal translocation (ST) when they reach their final destination in the cortical plate. This process is crucial for proper cortical layering and its perturbation can lead to brain dysfunction. Here we present a reductionist biomaterials platform that faithfully supports and controls the distinct phases of terminal ST in vitro. We developed microenvironments with different adhesive molecules to support neuronal attachment, neurite extension, and migration in distinct manners. Efficient ST occurred when the leading process of migratory neurons crossed from low-to high-adhesive areas on a substrate, promoting spr…
In Situ, Light-Guided Axon Growth on Biomaterials via Photoactivatable Laminin Peptidomimetic IK(HANBP)VAV
2018
The ability to guide the growth of neurites is relevant for reconstructing neural networks and for nerve tissue regeneration. Here, a biofunctional hydrogel that allows light-based directional control of axon growth in situ is presented. The gel is covalently modified with a photoactivatable derivative of the short laminin peptidomimetic IKVAV. This adhesive peptide contains the photoremovable group 2-(4′-amino-4-nitro-[1,1′-biphenyl]-3-yl)propan-1-ol (HANBP) on the Lys rest that inhibits its activity. The modified peptide is highly soluble in water and can be simply conjugated to -COOH containing hydrogels via its terminal -NH 2 group. Light exposure allows presentation of the IKVAV adhesi…
Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas.
2020
Purpose: Human cornea substitutes generated by tissue engineering currently require limbal stem cells for the generation of orthotypical epithelial cell cultures. We recently reported that bioengineered corneas can be fabricated in vitro from a heterotypical source obtained from Wharton’s jelly in the human umbilical cord (HWJSC). Methods: Here, we generated a partial thickness cornea model based on plastic compression nanostructured fibrin-agarose biomaterials with cornea epithelial cells on top, as an orthotypical model (HOC), or with HWJSC, as a heterotypical model (HHC), and determined their potential in vivo usefulness by implantation in an animal model. Results: No major side effects …
Saccharomyces uvarum is responsible for the traditional fermentation of apple chicha in Patagonia
2016
Apple chicha is a fresh low alcoholic beverage elaborated by aboriginal communities of Andean Patagonia (Argentina and Chile). In the present work, we identified the yeast microbiota associated with this fermentation, and characterized genetically those belonging to the genus Saccharomyces. Both Saccharomyces cerevisiae and S. uvarum were found in the analyzed fermentations. Phylogenetic and population structure analyses based on genes sequence analysis were carried out for both S. cerevisiae and S. uvarum strains obtained in this study and a set of additional strains from diverse origins. The results demonstrate that S. cerevisiae strains from apple chicha belong to the big group of wine/E…
A comprehensive assessment of energy efficiency of wastewater treatment plants: An efficiency analysis tree approach
2023
Producción Científica
Reprogramación directa de células somáticas adultas a células germinales meióticas
2014
El desarrollo de la línea germinal comienza en la especie humana durante el desarrollo embrionario con la aparición de las células germinales primordiales (CGP) desde el epiblasto proximal en la semana 2 a 3 de gestación. Las CGP migran a las crestas gonadales y cambian su perfil de expresión genético y epigenético desde un patrón somático predefinido a un patrón germinal. Durante el proceso de maduración germinal que depende del sexo del embrión, la adquisición del patrón gamético permite que células germinales puedan entrar en meiosis, un proceso crucial por el cual los gametos reducen a la mitad la carga cromosómica para, tras la fecundación, dar lugar a un nuevo individuo diploide. Sin …
Desarrollo de un modelo de trasplante traqueal alogénico in vivo en lagomorfo
2019
El principal reto en la patología de la vía aérea, tanto benigna como maligna es la limitación en la cantidad de tejido que puede ser resecado pudiendo lograr una restitución de la misma con seguridad, suponiendo esta una limitación absoluta para el tratamiento de gran cantidad de indicaciones que en nuestros días siguen sin una terapéutica efectiva. En el presente trabajo proponemos la sustitución de segmentos afectos de tráquea por injertos descelularizados de donante cadáver. Para ello, en modelo lagomorfo, a partir de tráqueas provenientes de animales control de otros estudios, se procede a su descelularización mediante el sometimiento a ciclos de exposición a detergentes (SDS), choques…
Biocompatibilidad y Bioactividad de nuevos materiales para Odontología Regenerativa
2021
Diversas situaciones patológicas dentales pueden verse beneficiadas de un tratamiento que favorezca la regeneración tisular. Las resorciones radiculares, las perforaciones iatrogénicas, la patología infecciosa periapical y las lesiones traumáticas de los dientes se asocian a una pérdida de tejido óseo peridentario. Los tratamientos restauradores convencionales pueden eliminar las circunstancias etiológicas con materiales que no solo sean biocompatibles sino que sean bioactivos. Un aspecto especial es el de la patología que afecta al complejo dentino-pulpar, la parte vital del diente. Se trata de un tejido similar al hueso (una zona mineralizada y otra no) pero con una distribución celular d…