Search results for "label"

Deficiency of Vitamin C in Dairy Products and Supplementation in Milk by Adding the Bioactive Natural Products

2011

Since milk constitutes an important item in the human diet and since the importance of an adequate amount of vitamin C in the diet is becoming more fully appreciated, it appeared to us that any investigation that might yield further information as to how milks containing greater quantities of this dietary essential might be produced would be worth while. Sea buckthorn is a natural multi-vitamins and a major source of vitamin C, fruits containing vitamin C at a rate of two times more than rosehips and ten times more than citrus (200-1500 mg). Strengthening with Vitamin C will be considering of content of vitamin C in milk samples taken per season (winter, spring, summer, autumn). This contri…

Highly efficient transport of carboxyfluorescein diacetate succinimidyl ester into COS7 cells using human papillomavirus-like particles

2003

AbstractHuman papillomavirus virus-like particles (VLPs) have recently been used to deliver genes into mammalian cells in vitro and in vivo. Here, we investigated whether VLPs may serve as an efficient carrier of low molecular weight compounds (e.g. hormones, vitamins, peptides etc.) into cells. COS7 cells were incubated with recombinant HPV-16L1/L2 VLPs labelled with the fluorescence dye carboxyfluorescein diacetate succinimidyl ester. Using flow cytometry, we demonstrate that labelled VLPs can specifically bind to the cell surface followed by their complete internalisation. Our results indicate that VLPs are promising vehicles for highly efficient delivery of low molecular weight compound…

Denaturing for Nanoarchitectonics: Local and Periodic UV-Laser Photodeactivation of Protein Biolayers to Create Functional Patterns for Biosensing

2022

[EN] The nanostructuration of biolayers has become a paradigm for exploiting nanoscopic light-matter phenomena for biosensing, among other biomedical purposes. In this work, we present a photopatterning method to create periodic structures of biomacromolecules based on a local and periodic mild denaturation of protein biolayers mediated by UV-laser irradiation. These nanostructures are constituted by a periodic modulation of the protein activity, so they are free of topographic and compositional changes along the pattern. Herein, we introduce the approach, explore the patterning parameters, characterize the resulting structures, and assess their overall homogeneity. This UV-based patterning…

Fluorescence labelling of organic acidic compounds with 4-bromomethyl-7-methoxycoumarin (Br-Mmc)

1977

4-Bromomethyl-7-methoxycoumarin (Br-Mmc) is introduced as a fluorescence marker for aromatic and heterocyclic acids. To investigate the applicability of this method on substances of different chemical classes, screening experiments with 110 compounds were carried out using a microrefluxer. Most aromatic and heterocyclic monocarboxylic acids gave Mmc-esters which are fluorescent on thin-layer plates, like the Mmc-esters of fatty acids, which have been previously investigated. Strong acids, alcohols, amides and most amines did not react, whereas certain cyclic amines such as piperidine gave strongly fluorescent derivatives. Mmc-phenyl ethers shows only weak fluorescence. A new standard proced…

Glucosylation of isatin-3-oxime followed by 2D in situ NMR in plant cells at highest magnetic field without labelling.

2001

The glucosylation of isatin-3-oxime (1) was monitored by in situ 2D 1H-13C inverse correlated gradient assisted NMR spectroscopy in plant cell suspension cultures of Rauvolfia serpentina without labelling. The applied high magnetic field of 800 MHz allowed measurements within 20 min at concentrations of 1 of 5.76 mM. Complete glucosylation of 1 occurs inside the cells within 72 hours. During this time isatin-3-oxime-glucoside (2) accumulates without further metabolism.

A standard calculation methodology for human doubly labeled water studies.

2021

Summary The doubly labeled water (DLW) method measures total energy expenditure (TEE) in free-living subjects. Several equations are used to convert isotopic data into TEE. Using the International Atomic Energy Agency (IAEA) DLW database (5,756 measurements of adults and children), we show considerable variability is introduced by different equations. The estimated rCO2 is sensitive to the dilution space ratio (DSR) of the two isotopes. Based on performance in validation studies, we propose a new equation based on a new estimate of the mean DSR. The DSR is lower at low body masses (<10 kg). Using data for 1,021 babies and infants, we show that the DSR varies non-linearly with body mass betw…

Studies on the Biosynthesis of Paraherquamide: Synthesis and Incorporation of a Hexacyclic Indole Derivative as an Advanced Metabolite

2001

Retinal Fundus Multi-Disease Image Dataset (RFMiD): A Dataset for Multi-Disease Detection Research

2021

The world faces difficulties in terms of eye care, including treatment, quality of prevention, vision rehabilitation services, and scarcity of trained eye care experts. Early detection and diagnosis of ocular pathologies would enable forestall of visual impairment. One challenge that limits the adoption of computer-aided diagnosis tool by ophthalmologists is the number of sight-threatening rare pathologies, such as central retinal artery occlusion or anterior ischemic optic neuropathy, and others are usually ignored. In the past two decades, many publicly available datasets of color fundus images have been collected with a primary focus on diabetic retinopathy, glaucoma, age-related macular…

A homemade cytospin apparatus

2006

Part-of-speech labeling for Reuters database

2015

Even if the Vector Space Model used for document representation in information retrieval systems integrates a small quantity of knowledge it continues to be used due to its computational cost, speed execution and simplicity. We try to improve this document representation by adding some syntactic information such as the parts of speech. In this paper, we have evaluated three different tagging algorithms in order to select the most suitable tagger for using it to tag the Reuters dataset. In this work, we have evaluated the taggers using only five different parts of speech: noun, verb, adverb, adjective and others. We considered these particular tags being the most representative for describin…