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RESEARCH PRODUCT
Photobleaching effects onin vivoskin autofluorescence lifetime
Inesa FerulovaJanis SpigulisAlexey Lihachevsubject
AdultTime FactorsMaterials scienceLightPhotochemistryBiomedical EngineeringHuman skinAbsorption (skin)LipofuscinBiomaterialsNuclear magnetic resonanceFlavinsIntradermal NevusmedicineHumansNevusskin and connective tissue diseasesPhospholipidsSkinInflammationPhotobleachingbusiness.industryEquipment DesignMiddle AgedHandmedicine.diseasePhotobleachingFluorescenceAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsAutofluorescenceSpectrometry FluorescenceOxyhemoglobinsPicosecondFlavin-Adenine DinucleotideNevus IntradermalOptoelectronicsbusinessdescription
The autofluorescence lifetime of healthy human skin was measured using excitation provided by a picosecond diode laser operating at a wavelength of 405 nm and with fluorescence emission collected at 475 and 560 nm. In addition, spectral and temporal responses of healthy human skin and intradermal nevus in the spectral range 460 to 610 nm were studied before and after photobleaching. A decrease in the autofluorescences lifetimes changes was observed after photobleaching of human skin. A three-exponential model was used to fit the signals, and under this model, the most significant photoinduced changes were observed for the slowest lifetime component in healthy skin at the spectral range 520 to 610 nm and intradermal nevus at the spectral range 460 to 610 nm.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-01-30 | Journal of Biomedical Optics |