The plasma membrane ATPase of Kloeckera apiculata: purification, characterization and effect of ethanol on activity

1994

Partially (6-fold) purified plasma membrane ATPase from an ethanol-sensitive yeast, Kloeckera apiculata, had an optimum pH of 6.0, an optimum temperature of 35°C, a K m of 3.6 mM ATP and a V max of 11 μmol Pi/min.mg protein. SDS-PAGE of the semi-purified plasma membrane showed a major band of 106 kDa. No in vivo activation of the ATPase by glucose was observed. Although 4% (v/v) ethanol decreased the growth rate by 50% it did not affect the ATPase. Concentrations of ethanol ≥2% (v/v) did, however, inhibit the enzyme in vitro. The characteristics of the enzyme did not change during growth in the presence of ethanol.

Discovery of the optical counterpart to the CGRO transient GRO J1008 - 57

1994

Triphenanthro‐anellierte [18]Annulene mit Alkoxy‐Seitenketten — Eine neue Klasse discotischer Flüssigkristalle

1994

Triphenanthro-Anellated [18]Annulenes with Alkoxy Side Chains - A Novel Class of Discotic Liquid Crystals Two different types 6 and 16 of [18]annulenes condensed with phenanthrene systems were prepared by cyclocondensation reactions (Schemes 2 and 3). The central 18-membered rings do not show a macrocyclic diamagnetic ring current. Conformational analyses and X-ray analyses in the solid state reveal non-planar structures in which aromatic “islands” are connected by (E)-configurated olefinic bridges. The introduction of long alkoxy side chains yields a new class of discotic liquid crystals; either ND or hexagonal columnar phases are generated, which were characterized by DSC, microscopy with…

Low prevalence of anti-HCV antibody among Italian children

1994

The seroprevalence of anti-HCV antibody was studied among 2,749 children and teenagers (1,438 males and 1,311 females) living in Italy. Anti-HCV antibody testing was positive by both EIA and RIBA in ten (0.36%) subjects. The positivity rate increased with age, ranging from 0 among children less than 6 years of age to 0.8% among those aged 17-19 years chi 2 linear regression = 0.038). Anti-HCV prevalence ranged from 0.2% in northeastern regions and in Apulia to 0.6% in Sicily and Sardinia (p0.005), and no difference was seen between males (0.35%, C.I. 95%: 0.04-0.66) and females (0.38%, C.I. 95%: 0.04-0.66) (Fisher's exact test = 0.565). From these data it appears that in Italy HCV infection…

Nutrient intake of endurance runners with ovo-lacto-vegetarian diet and regular western diet

1994

During an endurance run (1,000 km in 20 days) it was investigated whether an ovo-lacto-vegetarian diet (OLVD) could cover the nutritional requirements of endurance athletes. A regular western diet (RWD) was used as reference. Both diets were offered with an energy content of 4,500 kcal per day and an energy percentage of carbohydrate:fat:protein of 60:30:10. The runners were divided into two dietary groups according to their usual dietary habits. The results of the 55 participants who completed the race show that runners from both groups had the same intake of energy, carbohydrate, fat and protein. Runners of the OLVD group consumed more dietary fiber and polyunsaturated fatty acids as well…

Dosage du sotolon au cours de l'élaboration des vins jaunes du Jura

1994

National audience

Recovery of human fibroblasts from attack by the pore-forming alpha-toxin of Staphylococcus aureus.

1994

When applied at low concentrations (10 micrograms/ml), staphylococcal alpha-toxin generates a small channel in keratinocyte and lymphocyte membranes that permits selective transmembrane flux of monovalent ions. Here we show that a moderate concentration (1-50 micrograms/ml) of alpha-toxin similarly produces a small pore in membranes of human fibroblasts. This process leads to rapid leakage of K+ and to a drop in cellular ATP to 10-20% of normal levels in 2 h. In the presence of medium supplemented with serum and at pH 7.4, the cells are able to recover from toxin attack, so that normal levels of K+ and ATP are reached after 6-8 h at 37 degrees C. The repair process is dependent on the prese…

Prenatal diagnosis and carrier detection in mucopolysaccharidosis type II by mutation analysis. A 47,XXY male heterozygous for a missense point mutat…

1994

Identification of iduronate-2-sulphatase (IDS) gene mutations in patients with mucopolysaccharidosis type II (MPS II, Hunter syndrome) allows fast and reliable carrier detection and prenatal diagnosis. We describe here three cases of prenatal diagnosis by direct detection of the gene mutation. In addition to two affected male fetuses from two different families, a 47,XXY fetus carrying both the normal and the mutant allele was diagnosed in a third family. The latter pregnancy was carried to term and the child is obviously not affected by MPS II.

The pion polarisability from QCD sum rules

1994

Abstract The electromagnetic polarisability of charged pions, α E , has recently attracted both theoretical and experimental attention. Unfortunately the experimental results disagree with each other. We have investigated this polarisation via a QCD sum rule approach and find α E = 5.6 ± 0.5 × 10 −4 fm 3 , which is in agreement with one experiment and disagrees with the result of chiral perturbation theory.

Experimental and numerical modelling of the effects of process parameters in the Aquadraw deep Drawing

1994

International audience

Functional Liquid Crystalline Polymers: Ferroelectric Polymers and Liquid Crystalline Elastomers and Ionomers

1994

Abstract The incorporation of different functional groups in liquid crystalline polymers opens the way to a variety of interesting new materials, which may be used for non-linear optics, as piezo-elements or as two-phasic composite materials.

Electric Polarization of Onsager Fluids. II. Birefringence. 2. Molar Kerr Constants of Binary Solutions

1994

Die Verwandlung der senecanischen Tragödie in Marc-Antoine Murets “Julius Caesar” und Jacques Grévins “César”

1994

In 1547 Marc-Antoine Muret wroteJulius Caesar, the first tragedy by a French author with an exclusively historical plot. While imitating the dramatic structure of Seneca's tragedies, he fundamentally changed the nature of the tragic conflict, transferring it from the character of the tragic hero to the clash of two basically positive principles, monarchy and Republican liberty. Muret's Caesar does not plan a revengeful crime as the Senecan heroes did; he is rather the successful statesman, ready to die after having won the victory over the entire world, and his apotheosis is soon fulfilled by the gods. On the other side are the Republican heroes Brutus and Cassius who succeed in killing the…

Entropy, transverse entropy and partitions of unity

1994

AbstractThe topological entropy of a transformation is expressed in terms of partitions of unity. The transverse entropy of a flow tangential to a foliation is defined and expresed in a similar way. The geometric entropy of a foliation of a Riemannian manifold is compared with the transverse entropy of its geodesic flow.

Operative Hysteroscopy Ten Years' Experience

1994

Correlation between the Ionicity Character and the Charge Density in Semiconductors

1994

Using the electronic charge densities obtained by the empirical pseudopotential method for tetrahedrally bonded semiconductors, an ionicity scale is established that is in good agreement with the Phillips ionicity scale.

Development of Planning in Relation to Age, Attention, Simultaneous and Successive Processing

1994

This study investigated the development of planning and its relationship with age and other cognitive processes taken from the PASS model. Ninety-nine students in grades two, four, and eleven were administered planning, simultaneous processing, successive processing, and attention tasks. MANOVA with grade level and gender as independent variables revealed that grade level had a significant effect on planning, whereas gender did not. All but one planning variable showed a significant main effect of grade in subsequent ANOVAs. Pair-wise comparisons between grade levels indicated that the developmental trajectories were not uniform in different tasks. Regression analyses indicated that the co…

Endoscopic Procedures through the Foramen Interventriculare of Monro under Stereotactical Conditions

1994

The foramen interventriculare of Monro is an anatomical narrowness for the endoscopic access to the third ventricle. The effective mechanical angle to pass the foramen interventriculare from a frontal bore hole depends on the diameter in the plane of entrance, the depth of the foramen, and the diameter of the endoscope. Under the pathological conditions of a hydrocephalus internus the foramen interventriculare is enlarged. By means of stereotactical guidance, it is possible to reach the third ventricle accurately without damaging anatomical structures around the foramen interventriculare. The endoscopic technique under stereotactical guidance using a rigid endoscope is sufficient and safe t…

Connectionist models of face processing: A survey

1994

Abstract Connectionist models of face recognition, identification, and categorization have appeared recently in several disciplines, including psychology, computer science, and engineering. We present a review of these models with the goal of complementing a recent survey by Samal and Iyengar [Pattern Recognition25, 65–77 (1992)] of nonconnectionist approaches to the problem of the automatic face recognition. We concentrate on models that use linear autoassociative networks, nonlinear autoassociative (or compression) and/or heteroassociative backpropagation networks. One advantage of these models over some nonconnectionist approaches is that analyzable features emerge naturally from image-b…

Bipolar membranes under forward and reverse bias conditions. Theory vs. experiment

1994

Abstract Bipolar membranes are layered structures composed of one cation-and one anion-exchange membrane joined together in series. The current—voltage curves of two recently developed bipolar membranes have been analysed theoretically and experimentally under both forward and reverse bias conditions. The experimental trends observed are high conductivity under forward bias conditions and high impedance first, and then electric field enhanced (EFE) water dissociation for high enough applied voltages, under reverse bias conditions. The forward bias measurements can contribute to a better knowledge of some of the transport parameters entering also in the reverse biased membrane. Comparison of…

“Excess and Irony in Transatlantic Cable Poetry”

1994

The completion of the first successful transatlantic telegraph link in August 1858 was a major technological triumph which sparked an extreme reaction among the American public. Not only was the laying of the cable one of the most ambitious and costly engineering projects of the nineteenth century, but the celebrations in the United States which ensued in the weeks following the establishment of this instantaneous communications link between Europe and America were unprecedented and may well ...

Feedback Mechanisms between ?2M and TGF?1 Reduce Extracellular Matrix Synthesis of Liver Fat-Storing Cells

1994

Wage Drift: Phillips Curve vs Bargaining Models

1994

: The purpose of this paper is to shed light on the debate on market- versus bargaining-determined total earnings by examining whether models of wage drift based on wage-bargaining considerations empirically outperform models based on simple ad hoc formulations relating wage drift to excess demand for labour. The task is carried out by investigating the empirical performance of two bargaining models and two Phillips curve models in the context of data on the Finnish metal industry. The results suggest that the former perform better than the latter, thus providing support for the hypothesis that total earnings are bargaining-determined. Furthermore, the results are in line with the view that…

Translational and rotational molecular motion in supercooled liquids studied by NMR and forced Rayleigh scattering

1994

It has been shown that translational diffusion coefficients, Dt, in the supercooled van der Waals liquids, orthoterphenyl, phenolphthaleindimethylether, and salol, have a weaker temperature dependence than the shear viscosity, η, at T ≲ 1.2Tg and can be described by Dt ∼ η−χ with χ < 1 whereas Dr ∼ η−1 applies for the mean rotational diffusion coefficients, Dr, down to the glass transition temperature, Tg. This apparent decoupling of translational and rotational motion has been discussed in relation with possible anomalous short time diffusion, spatial heterogeneity, and cooperative molecular motions close to Tg.

Female reactions to male absence after pairing in the pied flycatcher

1994

Mating with an already mated pied flycatcher (Ficedula hypoleuca) male is costly for a female. Two hypotheses explain why some females still mate with already mated males. The deception hypothesis suggests that some females mate with already mated males since it is difficult to assess perfectly the mating status of a male with separate territories (polyterritoriality). The search cost hypothesis states that females are aware of male mating status but the costs of searching for an unmated male exceed costs associated with the status of secondary female. One potential cue that could disclose a male's mating status is the existence of brief visits to the primary territory by polyterritorial ma…

Snap-together vesicles

1994

Combined procedure for glaucoma and cataract: A retrospective study

1994

Abstract To ascertain the long-term effects of a triple procedure in the case of coexisting cataract and glaucoma, we did a retrospective study of 93 eyes that had cataract extraction, IOL implantation, and trabeculectomy within a ten-year period. Mean follow-up was 32.4 ± 21.9 months. Mean postoperative intraocular pressure (IOP) was significantly lower than preoperatively, even though fewer medications were being used. One year after surgery, 60% of eyes had a best corrected visual acuity of 20/40 or better with an average improvement of 3.8 Snellen lines. Patients who had had surgery four or more years before the study had the highest percentage of eyes with an IOP exceeding 22 mm Hg. Th…

Orientation and order in thin films of a combined liquid crystalline polymer

1994

The order in thin films of a combined liquid crystalline polymer is studied by X-ray reflection. Films of thicknesses of less than 200 nm on float glass are investigated as a function of temperature. The polymer with mesogenic groups in the main and side-chains exhibits smectic and cholesteric mesophases. Measurements in the smectic phases show a Bragg peak and smectic layers are oriented parallel to the substrate. The sample is thus macroscopically ordered by the influence of substrate and free surface. The film surface is very smooth after spincoating; surface roughness is typically 0.8 nm. First annealing of samples leads to a significant roughening of the free surface; roughness increas…

Vegetation changes and human action from the Neolithic to the Bronze Age (7000?4000 B.P.) in Alicante, Spain, based on charcoal analysis

1994

Charcoal analysis reveals various palaeo-ecological phases from the Neolithic to the Bronze Age. Agriculture starts about 7000 B.P. in favourable ecological conditions. Most of the charcoal spectra from sites on the coast represent thermomediterranean holm-oak forest; those from the inland mountains represent mesome-diterranean holm-oak forest. The Neolithic I Impressed Ware people were the first to clear the forest to plant their crops. This clearance of primary woodland resulted in the development of secondary vegetation of pine woods or scrub. The scrub reached its maximum during the Bell Beaker phase and Bronze Age in the Cova de les Cendres. In the Neolithic II open air sites, the perc…

Thermodynamics of Solubilization of Pentanol in Sodium Dodecyl Sulfate-Dodecyldimethylamine Oxide Mixed Micelles

1994

Abstract Heat capacity and density measurements of pentanol (PeOH)-sodium dodecyl sulfate (NaDS)-dodecyldimethylamine oxide-water mixtures were carried out at 0.03 m PeOH as a function of the total surfactants concentration (mc) at different ratios (XNaDS). From experimental data, the apparent molar volumes (VΦ,R) and heat capacities (CΦ,R) of PeOH in the surfactants mixture solutions were calculated. As a general feature, at a given mixture composition, VΦ,R increases monotonically with mt as observed in pure surfactants. In the cases of XNaDS = 0.1 and 0.3, VΦ,R drops at about 0.1 and 0.15 mt respectively. The decreasing CΦ,R VS mt curve shows peculiarities which were ascribed to the pres…

Silica gel chemistry

1994

X-ray powder diffraction pattern for lactitol and lactitol monohydrate

1994

Diffraction patterns were recorded, and unit cell dimensions refined by the least-squares method, for lactitol and lactitol monohydrate. Refined unit cell parameters for lactitol are: a =7.622(1) Å, b = 10.764(2) Å, c = 9.375(1) Å, β= 108.25(1)° in space group P21, and those for lactitol monohydrate a =7.844(1) Å, b = 12.673(2) Å, c = 15.942(2) Å in space group P212121.

Domain shapes and monolayer structures of triple-chain phospholipids on water

1994

Two triple-chain phospholipid isomers were investigated at the air-water interface by means of fluorescence microscopy and grazing incidence X-ray diffraction (GID). The two lipids differ only in the position of the branched chain at the glycerol backbone. Fluorescence microscopy shows different domain sharp-edged domains. In the case of dendritic domains the chains are more tilted, the deviation from hexagonal symmetry is more pronounced and hence the lattice anisotropy is larger.

Molecular dynamics of discotic charge-transfer complexes, dielectric spectroscopy and2H NMR studie

1994

Abstract Using a combination of solid state 2H NMR spectroscopy on selectively deuteriated samples and dielectric spectroscopy, the molecular dynamics of discotic charge-transfer (CT) complexes were investigated. These complexes show particular thermodynamic and flow properties. Considered were mixtures of low molar mass donors and acceptors, low molar mass donors with main chain acceptor polymers and covalently linked donor-acceptor twins with different lengths of the spacer. A main result is that correlated rotational motions of discotic molecules or groups about the columnar axis are observed in all systems except for the twin with the short spacer. This type of motion seems to be a gene…

Glycogen phosphorylase from flight muscle of the hawk moth, Manduca sexta: purification and properties of three interconvertible forms and the effect…

1994

Glycogen phosphorylase (EC 2.4.1.1) of Manduca sexta flight muscle was separated into three distinct peaks of activity on diethylaminoethyl-Sephacel. The three fractions of phosphorylase activity were further purified by affinity chromatography on AMP-Sepharose and shown to have the same relative molecular mass (=178000) on polyacrylamide gradient gel electrophoresis under non-denaturating conditions and to produce subunits of molecular mass =92000 on SDS gelelectrophoresis. On the basis of their kinetic properties with respect to the activator AMP and the inhibitor caffeine, the three fractions of phosphorylase activity were assigned as follows: peak 1=phosphorylase b (unphosphorylated for…

SULFUR ANALOGUES OF POLYCHLORINATED DIBENZO-p-DIOXINS, DIBENZOFURANS AND DIPHENYL ETHERS AS INDUCERS OF CYP1A1 IN MOUSE HEPATOMA CELL CULTURE AND STR…

1994

Three sulfur-containing compounds, 2,3,7,8-tetrachlorothianthrene (TCTA), 2,3,7,8-tetrachlorodibenzothiophene (TCDT), and 3,3[prime],4,4[prime]-tetrachlorodiphenyl sulfide (TCDPS), were analyzed for their CYP1A1-inducing potencies--measured as aryl hydrocarbon hydroxylase (AHH) and 7-ethoxyresorufin O-deethylase (EROD) activities--in mouse hepatoma cell culture Hepa-1. Marked differences in the induction potencies were observed among the three compounds studied and between 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and its sulfur analogue. The estimated EC50 values for TCDD, TCTA, and TCDT were about 8 pM, 700 pM, and 7.5 nM, respectively. TCDPS did not elicit any AHH/EROD induction. Compar…